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Citations for Millipore Sigma :
1451 - 1500 of 1800 citations for TMB Substrates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... rewashed in TBST and covered with a DAB peroxidase chromogen solution (SigmaFAST 3,3’-Diaminobenzidine peroxidase substrate, Sigma-Aldrich) until the complete appearance of the protein bands of interest ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was achieved using appropriate horseradish peroxidase-labeled secondary antibodies and Immobilon Western chemiluminescent HRP substrate (Millipore Sigma). Band intensity was determined with ImageJ 2.0.0-rc-69/1.52p software (http://imagej.nih.gov/ij ...
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bioRxiv - Neuroscience 2021Quote: ... the membrane was placed in deionized water and gently dried before adding chemiluminescent HRP substrate (EMD Millipore, WBKLS0100) for visualizing proteins ...
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bioRxiv - Neuroscience 2020Quote: ... was used as a secondary detection antibody and immunoblots were developed with Forte HRP substrate (Millipore, Dachstein, France). Anti-Actin antibody m25 (Merck ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were incubated with the appropriate secondary antibody and detected with Luminata™ Crescendo Western HRP Substrate (Millipore) according to manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... with 100µL of buffer solution (pH 4.3) containing 0.5 mM of 4-methylumbelliferyl-β-D-galactopyranoside substrate (Sigma). A kinetic analysis was performed at 37°C for 1-2 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were washed again with TBST three times and visualized with chemiluminescence using HRP substrate (Millipore, #WBKLS0500) or Pico (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... and signals were visualized by Luminata Forte chemiluminescence Western blotting substrate as per the manufacturer’s protocol (EMD Millipore). Antibodies used were rabbit anti-PAK4 #3242 (IB ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HA9 (inhibitor of the substrate binding domain of Hsp70) and brefeldin A (disrupts Golgi apparatus, Sigma-Aldrich, Merck) were added for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG antibodies and the Chemiluminescent Peroxidase Substrate (Sigma-Aldrich, 34578) were used ...
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bioRxiv - Cancer Biology 2020Quote: ... Detection was performed using horseradish peroxidase conjugated secondary mouse or rabbit antibody and chemiluminescence HRP substrate (Millipore #WBKLS0100).
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bioRxiv - Physiology 2020Quote: ... Substrate concentrations were kept constant except for the substitution of unlabeled metabolites with either 13C6 glucose (Sigma-Aldrich), 2,3-13C2 pyruvate (Cambridge Isotope ...
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bioRxiv - Pathology 2020Quote: ... Membranes were washed three times as above and then developed with BCIP/NBT liquid substrate system (Sigma-Aldrich). The protein concentration of the FIV was determined using a BCA assay (Pierce #23227 ...
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bioRxiv - Bioengineering 2020Quote: ... the substrates were coated with a 15 μg/mL solution of laminin (Sigma-Aldrich, St. Louis, MO, USA) to enhance cell adhesion ...
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bioRxiv - Microbiology 2019Quote: ... ChiA-CTDE543M at 10 mg/ml in PBS were incubated with 90 ml of substrate at 0.4 mg/ml dissolved in Sigma buffer (A4855, pH 5.0) (Sigma) at 37°C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... the signal was detected by the enhanced chemiluminescence method (ECL) using the Immobilon western chemiluminescent HRP substrate (Millipore) and recorded by a Fuji LAS-4000 image reader ...
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bioRxiv - Immunology 2021Quote: ... which was detected using a fluorometric β-galactosidase substrate 4-methyl-umbelliferyl-β-D-galactopyranoside (“MUG”; M1633, Sigma).
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bioRxiv - Immunology 2020Quote: ... Plaques were then visualised by incubating the cells with ExtrAvidin peroxidase followed by 3 amino-ethylcarbazole substrate (Sigma).
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bioRxiv - Pathology 2021Quote: ... The membrane was washed three more times in TBST before addition of Immobilon Western Chemiluminescence HRP Substrate (Millipore) and visualization via film development ...
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bioRxiv - Cancer Biology 2021Quote: ... The HRP reaction was initiated by addition of 3,3’,5,5’-tetramethylbenzidine enzyme-linked immunosorbent assay substrate solution (Sigma) and terminated after 30 min by adding H2SO4 (2M ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of supernatant was mixed with 50 μL of the MPO substrate 3,3’,5,5’-tetramethylbenzidine (Sigma-Aldrich) in a 96-well microplate ...
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bioRxiv - Molecular Biology 2022Quote: ... The immunoreactivities of the primary antibodies were visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, Billerica, MA, USA) and recorded using an Ez-Capture Analyzer (ATTO ...
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bioRxiv - Bioengineering 2022Quote: ... the substrates were further treated with laminin solution (20 μg ml−1 in NbActiv4 medium, L2020, Sigma, USA) for 30 minutes to promote cell adhesion and neurite outgrowth.60 After decanting the excess laminin solution from the substrates ...
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bioRxiv - Biochemistry 2022Quote: Oligonucleotides for preparing synthetic fluorescently labelled (Cy3 or FITC) RNA/DNA substrates were purchased from Sigma (HPLC purified) and their sequences are available in Supplementary Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... and leucine-aminopeptidase using a 7-amino-4-methylcoumarin (AMC)-substrate (L-leucine-AMC, Sigma-Aldrich, Darmstadt, Germany). Artificial substrates were added to the homogenate at a final concentration of MUF-glucosidase ...
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bioRxiv - Cancer Biology 2022Quote: ... and were either visualized with a LI-COR Odyssey scanner or using Immobilon Western Chemiluminescent HRP Substrate (Sigma) and visualized by autoradiography.
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bioRxiv - Molecular Biology 2022Quote: ... SM and trunSM (tissue lysates) and 14-3-3 was performed using Immobilon Western chemiluminescent HRP substrate (Millipore) and an ImageQuant LAS 500 imager (Cytiva Life Sciences) ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.5 mM SCFA substrate (sodium acetate, sodium butyrate, sodium propionate, or crotonic acid; all Sigma Aldrich, Germany); all powders were solubilized in ddH2O ...
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bioRxiv - Microbiology 2023Quote: ... Protein was detected using HRP-conjugated anti-mouse secondary antibody (Amersham) and Luminata Forte Western HRP substrate (Millipore).
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bioRxiv - Molecular Biology 2022Quote: ... Immunodetection was performed using a Luminata Forte Western HRP Substrate system (Merck Millipore, Burlington, USA, 61-0206-81) or a Chemi-Lumi One L system (Nacalai Tesque ...
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bioRxiv - Immunology 2023Quote: ... for 1 hour and then alkaline phosphatase substrate solution containing 4-nitro-phenyl phosphate (N2765-50TAB, Sigma-Aldrich) for approximately 30min ...
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bioRxiv - Cell Biology 2023Quote: ... The gel was then immersed in phosphate buffer supplemented with tyrosinase substrate L DOPA (Sigma Chemicals, Bangalore, India). Enzyme activity corresponded to the formation of black-color pigment.
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bioRxiv - Physiology 2023Quote: ... prior to imaging with a BioRad ChemiDoc Imager with Immobilon ECL Ultra Western HRP Substrate (WBKLS0500, Sigma-Aldrich). Images were analyzed using BioRad ImageLab software (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were washed and incubated in HRP-conjugated secondary antibody before detection using Immobilon ECL Ultra substrate (Millipore).
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactivity was detected by enhanced chemiluminescence solution (Bio-Rad Clarity Western ECL Substrate and ImmobilonTM western, Millipore, USA) and scanned by the gel documentation system (Bio-Rad chemidoc XRS plus).
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bioRxiv - Developmental Biology 2023Quote: ... Chemiluminescence was imaged on BioRad ChemiDoc gel imager with ∼800 ul Immobilon Crescendo Western HRP substrate (WBLUR0100, Millipore). Blots were treated with Restore™ Western Blot Stripping Buffer (21059 ...
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bioRxiv - Cell Biology 2022Quote: ... allowing for detection of the secondary HRP antibody detected using a chemiluminescence peroxidase substrate kit (Sigma, #CPS-1) and subsequent detection of the GAPDH loading control detected using its laser based infrared detection system ...
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bioRxiv - Microbiology 2023Quote: ... were used at a 1/10000 dilution before reaction with Immobilon Forte Western HRP substrate (WBLUF0100, Merck Millipore). Signals were acquired with Fusion FX (Vilber).
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bioRxiv - Developmental Biology 2023Quote: ... the membranes were treated in the dark with the reagent “Immobilon Forte Western HRP substrate” (Sigma Aldrich, WBLUF0100) and visualized at ChemiDoc instrument (BIO RAD).
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bioRxiv - Cell Biology 2024Quote: ... HRP-conjugated secondary antibody at room temperature for 1 h and developed using ECL substrate (Cat# WBKLS0500, Millipore).
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bioRxiv - Cancer Biology 2024Quote: ... The signal was detected by the Pro e-BLOT Touch Imager using chemiluminescent substrate (WBKLS0500; Merck Millipore, Boston), and the band intensity was measured using ImageJ software (National Institutes of Health).
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bioRxiv - Molecular Biology 2024Quote: Membranes were developed for 1-5 seconds using an enhanced chemiluminescence reagent (Luminata Forte HRP substrate; Millipore Sigma) in a gel documentation system (ChemiDoc Touch ...
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bioRxiv - Immunology 2021Quote: ... 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium (BCIP /NBT) liquid substrates for AP-enzyme (SIGMA) were added for overnight incubation at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Catalog no.115-035-174 Jackson Immuno Research Laboratory) by immunoblot analysis with Luminata Forte Western HRP substrate (Millipore).
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bioRxiv - Neuroscience 2022Quote: ... AP-Sema3A concentration was determined by AP activity assay (using AP substrate buffer para-nitro-phenyl phosphate, Sigma Millipore) (Mlechkovich et al. ...
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bioRxiv - Neuroscience 2022Quote: ... AP-Sema3A concentration was determined by AP activity assay (using AP substrate buffer para-nitro-phenyl phosphate, Sigma Millipore) (Mlechkovich et al. ...
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bioRxiv - Immunology 2019Quote: ... The binding was revealed with 0.5 mg/mL o-phenylenediamine dihydrochloride substrate (OPD) (Sigma-Aldrich, St Louis, MO, USA), and Optical Density (OD ...
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bioRxiv - Biochemistry 2019Quote: Inhibition of WT LcαE7 and the Gly137Asp LcαE7 variant was determined by a competition assay between the native-substrate analogue 4-nitrophenol butyrate (4-NPB, Sigma) and the boronic acid compounds ...
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bioRxiv - Cancer Biology 2020Quote: ... plates were washed 10 times with PBS/Tween and substrate was added (o-phenylenediamine dihydrochloride tablets) and then quenched with 0.2 N sulfuric acid (Sigma) after colour appears visible ...
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bioRxiv - Biochemistry 2020Quote: ... 7-amido-4-methyl-coumarin (NHMec) from the commercially available substrate Leucine-7-amido-4-methyl-coumarin (Sigma-Aldrich). For processivity assays ...