Labshake search
Citations for Millipore Sigma :
1751 - 1800 of 10000+ citations for 6 7 Dichloro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 5 min with 10 μg/ml 4’,6-Diamidino-2-phenylindole (DAPI; Sigma-Aldrich) to stain cell nuclei ...
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bioRxiv - Neuroscience 2022Quote: ... with 1:30,000 of the fluorescent marker of nucleic acids 4’,6-diamino-2-fenilindol (DAPI; Sigma-Aldrich). We took photographs of the cells using an epifluorescence microscope (DMIL LED ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were lastly counterstained with DAPI (4′,6-diamidino-2-phenylindole) and mounted on slides using DABCO (Sigma). Cells were then viewed using a Nikon Ti2-E/A1R-Multiphoton microscope equipped with DS-Qi2 camera (Nikon).
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bioRxiv - Microbiology 2022Quote: ... Nuclei were labeled using 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, catalog no. D9542, 100 ng/ml).
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bioRxiv - Neuroscience 2022Quote: ... Sections were then treated with 4′,6-Diamidine-2′-phenylindole dihydrochloride at 15mM (DAPI, Sigma-Aldrich, Madrid, Spain) during 15 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... washed 2 x in Gurr buffer and stained with 30% Giemsa stain solution (Sigma-Aldrich, #51811-82-6) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Nuclei were labeled using 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, catalog no. D9542, 100 ng/ml). The immunostained lymph node tissues were scanned using an Olympus VS-120 slide scanner with a Hamamatsu ORCA-R2 C10600 digital camera ...
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bioRxiv - Microbiology 2023Quote: ... NR-6226C (Terpyridine (2,2′:6′,2′′-Terpyridine, 98%)) was purchased from Sigma Aldrich (now Merck, KGaA, Darmstadt Germany). Metal solutions containing 2 µM ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated in blocking buffer containing 0.8 μg/ml DAPI (4’,6-diamidino-2-phenylindole, Sigma, cat # D9542) for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... nuclei were stained with 4ʹ,6-diamidino-2-phenylindole (DAPI) and mounted in Mowiol (Sigma-Aldrich, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The plate was then rinsed with PBS and counter-stained with 4′,6-diamidino-2-phenylindole DAPI (Sigma) for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were incubated with DAPI (4’,6-Diamidino-2-phenylindole dihydrochlorid) solution(D9542, Sigma-Aldrich, diluted 1:10000) for 7 minutes at RT and subsequently mounted in ProLong Gold Antifade mounting medium (cat ...
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bioRxiv - Genetics 2023Quote: ... were used as secondary antibodies and sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Mounting was performed using Prolong Gold Antifade Mountant (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Enriched proteins were denatured in U/T buffer (6 M urea, 2 M thiourea, 1 mM DTT (Sigma), 10 mM HEPES ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were routinely passaged in a 1:6 ratio every 2 days using 1× Trypsin–EDTA solution (Sigma) at 37°C and the reaction was stopped using 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated in the DNA fluorescent stain DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich, D9542) at a concentration of 1 µg/ml in 1x PBS for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 0.04 mg/ml catalase (EMD biosciences) and 1 mM 6-hydroxy-2,5,7,8-tetramethyl-chromane-2-carboxylic acid (Trolox) (Sigma Aldrich). After DNA was tethered on the surface ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed and the nuclei counter stained with 40-6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich) for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... were used as secondary antibodies and sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Mounting was performed using Prolong Gold Antifade Mountant (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) 1:1,000 (Sigma-Aldrich Sweden AB, Stockholm, Sweden). Invasion of the cells was measured by counting the nuclei of the cells that migrated through the filter pores towards the chemoattractant (6% FBS) ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant became the soluble fraction and the pellet was solubilized in urea buffer (30 mM Tris, 7 M Urea, 2 M Thiourea, 4% CHAPS, 1X Protease Inhibitor Cocktail (Millipore Sigma 11873580001), 0.5 mM PMSF ...
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bioRxiv - Neuroscience 2021Quote: ... during 7 days, in a medium composed of equal volumes of modified C2C12 differentiation medium (DMEM, 2% horse serum (Sigma-Aldrich, #H1270),1% antibiotic-antimycotic (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Oral microgavage of 6 dpf larval zebrafish was performed using pH indicators m-cresol purple (0.2%; Thermo-Fisher, 2303-01-7) or phenol red (2%; Sigma, 143-74-8). The fish were then imaged on a Leica MZ10F stereoscope using a Leica MC190 HD color camera ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral supernatants were generated by transfecting the plasmids into 293T cells and used to infect 8988T cells that were then selected for at least 7 days with 2 μg ml−1 puromycin (Sigma-Aldrich, P8833). The RNAi consortium clone IDs for the hairpins used were ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% galactose [Sigma-Aldrich #G0625] ...
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bioRxiv - Molecular Biology 2022Quote: ... Stimulation of Jurkat cells for 2-7 hour experiments was achieved with 50ng/ml Phorbol 12-myristate 13-acetate (PMA, Sigma-Alrich, P8139) and 500ng/ml ionomycin calcium salt from Streptomyces conglobatus (ionomycin ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 1 ml of RPMI + 2% Bovine Serum Albumin (BSA, heat shock fraction, protease free, essentially globulin free, pH 7, Sigma, Catalog #A3059) to a concentration of ∼2 x 108 CFU/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were fixed for 7 minutes in 2% formaldehyde/0.2% glutaraldehyde solution at room temperature and further incubated with X-Gal (Sigma, Cat. no. B4252) solution for one hour at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... individuals were placed in a microtube containing in 100 µl of either Schneider’s media (Experimental Replicates 1 and 2) or RPMI media (Experimental Replicates 3 and 4) to which 2% Penicillin/Streptomycin and Amphotericin B (Sigma-Aldrich, Dorset, UK) had been added ...
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bioRxiv - Zoology 2020Quote: ... 3-methyl-but-2-enyl acetate and (2E)-hex-2-enyl acetate were confirmed with analytical standards from Sigma-Aldrich (St. Louis, MO). For the analysis of CHCs ...
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bioRxiv - Microbiology 2022Quote: ... diluted in DMSO and 6-Thioguanine (6-TG) (Sigma-Aldrich) diluted in 1M NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... and glucose-6-phosphate dehydrogenase (G-6-PDH; Sigma-Aldrich). For enhanced chemiluminescence detection (ECL) ...
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bioRxiv - Cancer Biology 2023Quote: ... Dextran T-500 (Sigma-Aldrich; 6 mL, 6% in saline) was added to the cells and filled up to 50 mL with 0.9% saline ...
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bioRxiv - Microbiology 2023Quote: ... 5 mg/mL 6-animopenicillanic acid (6-APA) (Sigma-Aldrich), 5 mg/mL 7-aminocephalosporanic acid (7-ACA ...
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bioRxiv - Microbiology 2022Quote: The AHL signaling molecules used in this study are N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL, Sigma-Aldrich CAS# 168982-69-2), N-butyryl-homoserine lactone (C4-HSL ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then dehydrated in ascending concentrations of ethanol for 5 min each (2[×[35%, 50%, 70%, 80%, 90%, 3[×[100%) and washed 3 times for 5 min with propylene oxide (Sigma-Aldrich, #cat 110205-18L-C). Next ...
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bioRxiv - Developmental Biology 2019Quote: ... pH 6 (Sigma) at 95 °C for 20 minutes then room temperature for 20 minutes prior to staining ...
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bioRxiv - Plant Biology 2020Quote: ... the first aliquot was derivatized using cysteamine (0.25 M; pH 8; 1h; room temperature; Sigma-Aldrich), the second aliquot was immediately further processed as following ...
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bioRxiv - Immunology 2021Quote: ... for 1h with or without a pretreatment with 20 μM MCC950 (Millipore Sigma, Cat. no: 538120) for 1h prior to activation ...
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bioRxiv - Immunology 2021Quote: ... for 1h followed by NLRP3 activation with 20 μM nigericin (Sigma-Aldrich, Cat. no: N7143-5MG) for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... with prior 1h incubation of the biotinylated oligonucleotide with the anti-G4 antibody BG4 (Merck Millipore).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was administered 30 minutes before the stimulus with NMDA (30μM for or 1h; Sigma–Aldrich, #M3262), dexamethasone (1 μM for 1h or 24h ...
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bioRxiv - Genomics 2021Quote: ... the membranes were incubated for 1h with the secondary antibody goat anti-rabbit-HRP (Sigma, G9545) 1:10,000 in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a blocking step for 1h with 5% normal donkey serum (NDS) (D9663, Sigma Aldrich) in 0.1% PBS-Triton X-100 (PBS-T) ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.6) and incubated for 1h with primary antibodies (1:4,000 dilution, anti-flag mouse -Sigma #F1804 or anti-myc mouse - Cell Signaling #2276) ...
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bioRxiv - Microbiology 2023Quote: ... followed by a 1h-incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore). Beads were washed 3 times with GTEN buffer ...
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bioRxiv - Cell Biology 2023Quote: ... slides were incubated for 1h at RT in blocking buffer containing 10% goat serum (Sigma, G9023) with 0.03 % triton X-100 in PBS and 1:50 Affini Pure Fab Fragment Donkey Anti-Mouse IgG (Jackson Immuno Research Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... determined using quantitative 1H NMR spectroscopy using TraceCERTS® ethyl 4-(dimethylamino)benzoate from Sigma Aldrich as internal calibrant (Cushman et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... secondary antibody was incubated at room temperature for 1h followed by DAPI nuclear staining (Sigma, Germany). Immunostainings were examined with an immunofluorescence microscope (Leica) ...
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bioRxiv - Cell Biology 2020Quote: ... 7′ -dichlorofluorescin diacetate (DCFH-DA; Sigma, USA) after enzymatic digestion of minced tissue (20) ...