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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... mouse IgG1 anti Golgin 97 (CST) or mouse anti IgG1 PDIA3 (Sigma-Aldrich) and mouse IgG2a anti-dsRNA J2 (Scicons ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-mouse α-smooth muscle actin (αSMA) conjugated to Cy3 fluorophore (Sigma), and mouse anti-rat neural-glial antigen 2 (NG2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Mouse anti-myc antibody (1 μg) or mouse IgG (1 μg) (EMD Millipore) were incubated with protein A/G Magna beads (20 μl ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse Vinculin (V9131) and mouse Flag (F3165) antibody were purchased from Sigma-Aldrich. Antibodies against METTL14 (26158-1-AP) ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-FLAG (M2) and mouse anti-α-tubulin (clone DM1A) (Sigma-Aldrich); mouse anti-clathrin heavy chain (BD Biosciences) ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-HA Mouse monoclonal antibody (Clone HA-7, mouse IgG1 Isotype; Sigma-Aldrich). The membrane was washed thrice in PBST and incubated with HRP-conjugated secondary antibody (anti-rabbit and anti-mouse ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells were routinely passaged in Dulbecco’s modifies Eagle medium (DMEM+) culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... were inserted between the NdeI and XhoI sites of the bacterial expression vector pET-24a containing a HIS-tag (Novagen, Madison, USA). The ligated products were transformed into TOP10 E ...
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Microbiology 2023Quote: ... then concentrated using 15 ml Amicon 100 kDa centrifuge filter columns and purified by nickel column affinity chromatography (Novagen His-Bind Resin) as per the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C in primary antibody diluted in blocking solution (mouse anti-CamKII from Millipore 05-532 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then labelled with different sets of primary and secondary antibodies: mouse anti-HA antibody (1:1000, Sigma), mouse anti-FLAG (1:1000) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with rabbit anti-mouse IgG-FITC polyclonal antibody at 1:500 (F9137, Sigma, Milan, Italy) in blocking solution at RT for 2h and counterstained with 0.5μg/mL PI at RT for 5 min ...
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bioRxiv - Genomics 2021Quote: ... S9.6-treated or anti-FLAG treated cells were incubated with 1:100 dilution of anti-mouse antibody (Sigma, M7023) in 200µl of dig-wash buffer at room temperature for 1 hour by rotating.
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bioRxiv - Genomics 2021Quote: ... Cells were pelleted again and resuspended in 50 µL of permeabilization buffer with 2% mouse serum (Millipore Sigma, M5905) to block for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse myoblast C2C12 cells (gift from Edgar R. Gomis, iMM, Portugal) were grown in DMEM High Glucose (Sigma Aldrich) supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated with 381.7 ng / ml mouse α-HAdV hexon protein antibody (Mab8052, Sigma-Aldrich, St. Louis, USA) and subsequently stained using 2 μg / ml goat α-mouse-AlexaFluor594 (A21203 or A32742 ...
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bioRxiv - Cell Biology 2022Quote: E14 mouse ES cells (male, RRID: CVCL_C320) were cultured in the serum/LIF medium containing Glasgow Minimum Essential Media (Sigma), 10% heat-inactivated FBS (HyClone) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1987)) and Rad54-/- mouse ES cells (Essers et al., 1997) were cultured on gelatinized plates (0.1% porcine gelatin (Sigma)) ...
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bioRxiv - Neuroscience 2021Quote: Rat and mouse primary cortical neurons and BE(2)-M17 cells were fixed using 4% paraformaldehyde (PFA, Sigma-Aldrich) in PBS pH 7.4 for 20 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were incubated with monoclonal mouse ANTI-FLAG® M2 antibody (Sigma-Aldrich, F1804-50UG, dilution 1:100) overnight at 4°C and then with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse embryos were cultured until the 8-cell stage in KSOM medium supplemented with 0.4% bovine serum albumin (Millipore), after which the medium was changed to Cook Blasto ® (Cook Ireland Ltd ...
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bioRxiv - Developmental Biology 2022Quote: Mouse primary pancreatic cells were obtained by digesting the whole pancreas with 1 mg/mL Collagenase P (Sigma, 11213865001) supplemented with 2 U/mL Dispase II (Life Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... The mouse E14TG2a male ESC line originated from the European Collection of Authenticated Cell Cultures (Sigma 08021401, Lot #15H010). The E14TG2a ESCs were cultured in gelatin-coated (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with primary antibody in blocking solution (1:500 mouse anti-gamma tubulin (Sigma Aldrich, GTU-88), 1:3000 mouse anti-ARL13B (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: Human 293T and mouse P19 cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained for intracellular markers with anti-α-SMA (mouse IgG2a, Sigma Aldrich, St. Louis, MO, 1/250), anti-desmin (rabbit polyclonal IgG ...
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bioRxiv - Developmental Biology 2021Quote: ... 293T or AL1 cells were incubated for 3 hours with anti-Myc mouse monoclonal antibody (Sigma-Aldrich; 1:500), and secondary staining was performed using anti-mouse AlexaFluor568 antibodies (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: E14 mouse embryonic stem cells (ESCs) were grown in two different media: ‘serum+LIF’ consisted in Glasgow medium (Sigma), 15% FBS (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... while mouse primary astrocytes (~3.5 × 105 cells) were seeded on poly-L-lysine coated transwell filters (3450; Sigma-Aldrich) and maintained in astrocyte-media ...
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bioRxiv - Cell Biology 2022Quote: C2C12 mouse myoblasts were obtained from RIKEN BRC Cell Bank and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich) supplemented with 10 % (v/v ...
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bioRxiv - Immunology 2022Quote: ... the cells were immunostained with a mouse monoclonal antibody against SARS-CoV-1/2 nucleoprotein [clone 1C7C7 (Sigma-Aldrich)] ...
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bioRxiv - Genetics 2023Quote: Mouse embryonic stem cells (ESCs) were grown on 0.1% gelatin-coated culture dishes in Glasgow Minimum Essential Medium (Sigma) supplemented with 20% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... NIH/3T3 embryonic mouse fibroblast cells (ATCC CRL-1658) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM high glucose; Sigma D6329) supplemented with 10% fetal bovine serum (Sigma) ...
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bioRxiv - Genomics 2023Quote: E14 mouse embryonic stem cells (E14Tg2a.4) (RRID:MMRRC_015890-UCD) from Mutant Mouse Regional Resource Centers were maintained on 0.1% gelatin (Sigma-Aldrich G1393) coated 6-well plates under the serum/LIF condition containing 15% ES-grade FBS (Gibco 16141061) ...
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bioRxiv - Biophysics 2023Quote: ... The cells were then incubated in a 1:250 Anti-β-Tubulin mouse monoclonal antibody (Millipore-Sigma, T5293-.2ML)-NGS-PBS solution for 2 hours ...
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bioRxiv - Biophysics 2023Quote: ... The cells were then incubated in a 1:250 Anti-β-Tubulin mouse monoclonal antibody (Millipore-Sigma, T5293-.2ML)-NGS-PBS solution for 2 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse zygotes were isolated in FHM under mineral oil and cumulus cells were removed with hyaluronidase (Sigma-Aldrich; H4272). Mouse embryos were cultured in pre-equilibrated Global Media (Cooper Surgical ...
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bioRxiv - Microbiology 2023Quote: ... spleen cells from a single mouse were harvested and stimulated for 6 h with 16 nM PMA (Sigma-Aldrich) and 1 μM ionomycin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 1:500 dilution of rabbit anti-MeCP2 (Cell signalling) and 1:10000 dilution of mouse anti-vinculin (Sigma Aldrich). After the incubation with the primary antibody ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies (insulin rabbit monoclonal, Cell Signaling 3014, 1:3000 and glucagon, mouse monoclonal, Sigma-Aldrich G2654, 1:1000) were applied and incubated for 60 minutes followed by secondary antibodies ...
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bioRxiv - Immunology 2024Quote: ... the cells were incubated in a 1:500 dilution of 4D2 mouse anti-PLSCR1 antibody (Millipore Sigma, cat. #MABS483) in PBS-BG (1x PBS with 0.5% bovine serum albumin and 0.1% glycine ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated for 1 hr at 37°C with mouse anti-beta-Tubulin (#05-661; Millipore; 1:1500) primary antibody diluted in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... or NK92 cells with antibodies against acetylated (Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse, Sigma-Aldrich, Cat# T6793) and detyrosinated (Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker ...