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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for Recombinant Human CTLA4 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... mouse anti-human β-actin (Sigma, #A5441). Immunoblots were analyzed using horseradish peroxidase secondary antibodies ...
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bioRxiv - Immunology 2024Quote: ... along with 5% human AB serum (Sigma) (32–35) ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 5% human serum (Sigma-Aldrich) and 5% mouse serum (Sigma-Aldrich)] was added ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody bound DNA-protein complexes were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed and eluted ...
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bioRxiv - Bioengineering 2021Quote: Protein quantification using Micro BCA protein assay (Pierce™ BCA Protein Assay Kit, Sigma Aldrich, Poole, UK) was carried out following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane proteins were separated from cytosolic proteins by ultracentrifugation or a ProteoExtractR Transmembrane Protein Extraction Kit (Novagen). Protein concentration was measured with a bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was equally adjusted using Bradford protein assay (Sigma).
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bioRxiv - Synthetic Biology 2020Quote: ... Protein was concentrated in 10,000 MWCO Amicon protein concentrators (Sigma), protein concentration was assayed by Qubit ...
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bioRxiv - Genetics 2020Quote: ... Proteins were quantified by Bradford Protein Assay Kit (Sigma-Aldrich). Protein samples were separated by 4-12% Bis-Tris Protein Gels (Thermo Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was determined using Bradford protein assay reagent (Sigma), measuring absorbance at a wavelength of 595 nm on a UV/Visible spectrophotometer (Ultrospec 2100 pro ...
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bioRxiv - Cell Biology 2019Quote: ... Protein A and Protein G-Sepharose (Sigma-Aldrich, #P3391, #P3296), IgG sepharose 6 Fast Flow (#GE17-0969-01).
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bioRxiv - Biochemistry 2022Quote: ... and the protein stained using ReadyBlue protein stain (Sigma-Aldrich). Fluorescent DNA bands and protein bands were quantified using the Bio-Rad Image Lab software 6.0.1.
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bioRxiv - Molecular Biology 2024Quote: ... Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) were added to the mix followed by incubation at room temperature for 2 h ...
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bioRxiv - Bioengineering 2020Quote: ... Negatively selected human monocytes (Astarte Biologics) were suspended in RPMI 1640 supplemented with 2% human AB serum (Sigma-Aldrich), 1% GlutaMax (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 (human embryonic kidney) and U2OS (human bone osteosarcoma) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) supplemented with 10 % FBS ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney cells (HEK293T) and human fibroblast cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma Aldrich), supplemented with fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... Detection of IgG1 employed anti-human IgG (VH+VL) (Jackson) and of IgA2 anti-human IgA (Hc only) (Sigma). Commercial antibodies were used at dilutions recommended by manufacturer ...
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bioRxiv - Biophysics 2019Quote: ... Live CH27 cells in suspension were pelleted at 500g for 5 minutes at 4°C then incubated on ice for 10 minutes with 3.5μg/mL biotinylated transfer-rin (Sigma-Aldrich: T3915) in the live cell compatible buffer ...
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bioRxiv - Cell Biology 2021Quote: ... a biotinylated secondary antibody was used and was followed by a third incubation step with a streptavidin-linked horseradish peroxidase (Sigma), followed by washing 3 x 5 min with PBST ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation in 1:50 dilution of biotinylated anti-lucifer yellow antibody (RRID: AB_2536191) in universal antibody dilution solution (Sigma Aldrich) for 3 days at 4°C with occasional gentle agitation ...
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bioRxiv - Cell Biology 2019Quote: ... or incubations with species specific biotinylated antibodies (Vector, 1:100), then streptavidin-HRP antibody (Vector, 1:200) before development with diaminobenzidine (Sigma). Counterstaining was performed using toluidine blue ...
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bioRxiv - Neuroscience 2021Quote: ... The immunoreactivity was detected using the avidin-biotin detection method (biotinylated donkey-anti rabbit IgG (Sigma-Aldrich, Søborg, Denmark, #SAB3700966); avidin-biotin-peroxidase complex (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... and then hybridized for 1h at room temperature with biotinylated RNA probes that had been in vitro transcribed in the presence of biotin-16-UTP (Sigma) with T7 RNA polymerase (NEB ...
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bioRxiv - Physiology 2020Quote: ... Brains were then preincubated in 5% normal goat serum in PBT for 3 hours at room temperature with gentle agitation followed by incubation in 1:50 dilution of biotinylated anti-lucifer yellow antibody (RRID: AB_2536191) in universal antibody dilution solution (Sigma Aldrich) for 3 days at 4°C with occasional gentle agitation ...
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bioRxiv - Cell Biology 2020Quote: ... Biotinylated cells were lysed in 1% Triton X-100 containing lysis buffer followed by pulldown with Streptavidin-agarose beads (Sigma) overnight and western blot analysis with indicated antibodies.
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bioRxiv - Immunology 2022Quote: ... and 20ug/mL of biotinylated anti-KIR3DL3 (clone CH21; gift from Dr Ashley Moffett) and coaggregated with 20ug/mL Avidin (Sigma). Relative intracellular free calcium was measured over time using flow cytometry on a BD LSR Fortessa X-20 instrument and analyzed using FlowJo software (v10.8.1) ...
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bioRxiv - Cancer Biology 2022Quote: ... and additional wells with 20% biotinylated PLL-g-PEG and FNIII(7-10) were supplemented with 1 µM staurosporine (Merck Millipore) to serve as a positive control for apoptosis ...
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bioRxiv - Microbiology 2022Quote: ... the microarrays were overlaid with the VP1 proteins for 90 minutes as protein-antibody complexes that were prepared by preincubating VP1 with mouse monoclonal anti-polyhistidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 4:2:1 (by weight ...
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bioRxiv - Immunology 2023Quote: ... and 1µg/ml biotinylated detecting antibody (JES6-5H4) followed by labelling with 1:2000 diluted ExtrAvidin®−Peroxidase (Sigma E2886) and developed with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Scientific™) ...
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bioRxiv - Biochemistry 2023Quote: ... the microarray was overlaid with the VP1 protein for 90 minutes as a protein-antibody complex that was prepared by preincubating VP1 with mouse monoclonal anti-polyhistidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 4:2:1 (by weight ...
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bioRxiv - Cell Biology 2020Quote: Immunoprecipitation (IP) of Flag-tagged ULK1 construct was performed using EZviewTM Red ANTI-FLAG ® M2 Affinity Gel (Sigma-Aldrich, F2426) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% of pre-cleared chromatin was set aside for input and 5 µg Monoclonal ANTI-FLAG® M2 antibody (to capture FLAG-tagged dCas9) or IgG (Sigma) was added to the remaining volume and then incubated at 4°C on a nutator overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein lysates were diluted to 1 ml with CoIP Lysis Buffer and the 3xFLAG-tagged bait was immunoprecipitated by incubation with 20 μl of anti-FLAG M2 Magnetic Beads (Sigma M8823) for 2 hrs at 4 °C on a tube rotator ...
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Molecular Biology 2020Quote: Primer sequences for markers were obtained from the UCSC genome browser and standard HEX-tagged primers (Sigma-Aldrich, Supplementary Table 2) were used to assess the flanking haplotypes of MMP20 ...
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bioRxiv - Cell Biology 2022Quote: ... C-terminal FLAG-tagged OCT4 and various SOX TFs were generated by subcloning cDNAs into pFLAG-CMV5a mammalian expression vector (Sigma-Aldrich). For stable expression of mCherry and SOX15 in OEC-2 cells ...
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bioRxiv - Microbiology 2021Quote: ... The HS and CS samples were analyzed by liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) and quantified by inclusion of [13C6]aniline-tagged standard HS disaccharides (Sigma-Aldrich), as described (Lawrence et al. ...
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bioRxiv - Biochemistry 2021Quote: ... The blot was stripped and then reprobed for FLAG-tagged receptor using anti-FLAG antibody (1:2000, Sigma, Cat. no. A8592). Data were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Systems Biology 2020Quote: Mammalian expression vectors encoding Flag-epitope tagged full-length Csk and eGFP-tagged Ptpn18 WT & Y/F mutant were transfected into 293T cells using 5 ug/mL PEI (Polyethylenimine, Sigma Aldrich) in Opti-MEM (GIBCO) ...
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bioRxiv - Molecular Biology 2022Quote: Nuclear extracts from HEK 293 cells expressing either FLAG-tagged CXXC or PHD constructs were incubated with anti-FLAG M2 beads (Sigma Aldrich) in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... Degradation of the AID-tagged RAD21 was induced by the addition of 500 μM (final concentration) indole-3-acetic acid (IAA/auxin; Sigma Aldrich) during media change 24 hours prior to imaging if applicable.
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bioRxiv - Cell Biology 2020Quote: Purification of mammalian Cavin1 was performed by Transfecting GFP-tagged Cavin1 using polyethylenimine (PEI) transfection reagent (Sigma-Aldrich Cat. No. 408727) with 1:4 w/w ratio (DNA:PEI ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation of lysates of hTERT-RPE1 cells stably expressing HA-tagged WDR60 or WDR34 using anti-HA agarose beads (Sigma-Aldrich). Lysis buffer containing 50 mM Tris/HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... cell surface HS level was examined after staining with anti-HS antibody (10E4, amsbio) at 5 μg/ml and FLAG-tagged enzymes were detected by using anti-FLAG® M2 antibody (Sigma), at 2 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: HEK293 cells stably expressing FLAG-tagged D3R were grown in DMEM with 10% FBS in 6-well plates coated with 400 µl ECM (Sigma E1270) to 80-90% confluence ...
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bioRxiv - Plant Biology 2022Quote: ... resolved by SDS-PAGE and immunoblotted for the presence of the HA- tagged effector using α-HA antibody (H9658; Sigma-Aldrich) and α-HA-HRP (3F10 ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary substrate was horse radish peroxidase tagged anti-rabbit secondary antibody and anti-goat secondary antibody (1:2500, EMD Millipore). The bands were detected using a chemiluminescent substrate (SuperSignal West Pico ...
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bioRxiv - Biochemistry 2023Quote: Hexahistidine tagged hMDC1 BRCT (residues: 1891 – 2089) was cloned into pET47b vector and expressed in Rosetta™ 2(DE3) cell lines (Novagen). Cells in 1L Luria Broth (LB ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-PA-tagged antibody conjugated with a PLUS oligonucleotide was prepared using the Duolink In Situ Probemaker PLUS (DUO92009; Sigma-Aldrich). The specimens were washed twice with wash buffer A and were subsequently incubated with Duolink ligation solution for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... or BSA (as a control) were fluorescently tagged with a 568nm label using Mix-N-Stain CF 568 kit (Millipore Sigma). Unincorporated label was removed by a size filtration spin column and incorporation of the tag was checked by SDS-PAGE ...