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Citations for Millipore Sigma :
1551 - 1600 of 2320 citations for 8 IODOMETHYL QUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... cells were washed with ice-cold PBS and then lysed with freshly prepared lysis buffer composed of 8 M urea (Sigma), 0.1 M HEPES (pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... For immunofluorescence stainings slices were incubated for 1 h at room temperature (RT) in blocking buffer (2% gelatine, 2% BSA (bovine serum albumin, Cat#a2153, CAS Number: 9048-46-8, Sigma), 0.1% Triton X-100 (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... The harvested lentiviral supernatants were filtered using 0.45 μm syringe filter (Fisher) and used to infect target A549 cells (polybrene was added at a final concentration of 8 μg/ml, Sigma). To infect A549 cells for CRISPRi ...
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bioRxiv - Cell Biology 2020Quote: ... Equal protein concentrations were resolved via 8% SDS–PAGE and transferred to Immobilon-P polyvinylidene difluoride membrane (Sigma Aldrich; IPVH00010). Antibodies used ...
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bioRxiv - Neuroscience 2021Quote: ... and plated at a density of 1,000 cells per mm2 into 8-μwell plates (BioValley) coated with 50 μg/ml poly-D-lysine (Sigma Aldrich). Cultures were kept at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... HEC-1-B cells and HEK293T cells at the confluency of 70%∼90% were infected with the virus in the presence of 8 μg/ml polybrene (Sigma). Puromycin (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: Retroviral infection was conducted in 6-well plates using either 1 mL of unconcentrated virus or 200 μL of concentrated virus in 20 mM HEPES pH 7.4 and 8 μg/mL polybrene (Sigma-Aldrich). If selection was desired ...
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bioRxiv - Cell Biology 2022Quote: ... Arteries were then stimulated with increasing concentrations of serotonin (5-HT, from 10 -8 to 3· 10 -5 M, Sigma), endothelium-dependent vasodilator acetylcholine (ACh ...
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bioRxiv - Biophysics 2022Quote: ... of polyethylene glycol (PEG) of a range (0.4, 2, 4, 8, 20 or 35 kDa) of molecular weights (Sigma-Aldrich). GC376 (GC376 Sodium ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... mTEC were cultured from ALI day -3 to ALI day 8 with an added gamma-secretase inhibitor (DAPT, 50 μM, Sigma) or vehicle control (DMSO ...
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bioRxiv - Immunology 2022Quote: Monocytic THP-1 cells were infected at a density of 2×105 cells/ml in 48 well plates in the presence of polybrene (8 μg/ml, Sigma). Infection levels were assessed at 48 h post-infection through enumeration of GFP positive cells by flow cytometry ...
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bioRxiv - Genomics 2020Quote: ... cells were cross-linked with 1% fresh formaldehyde for 15 minutes and treated with lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.3% IGEPAL CA-630 (Sigma-Aldrich), 1X complete protease inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... 4×106 ESCs were allowed to attach to gelatinised 15cm dishes for 6-8 hours and treated with 1μM all-trans retinoic acid (Sigma-Aldrich) in EC-10 medium (DMEM supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2019Quote: ... pH 8) containing 3 mg/ml lysozyme (Chicken egg white; Fluka) and 1 mg/ml lipase (from Candida cylindracea; Sigma) in an Eppendorf tube ...
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bioRxiv - Biophysics 2019Quote: ... A pre-reaction mixture was prepared in MTEN buffer (5 mM MES (Sigma Aldrich, cat# 69889, CAS: 145224-94-8), 25 mM ethanolamine (Sigma Aldrich ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with antibodies (5-8 μg) overnight at 4°C followed by incubation for 3 hours with protein G-sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Cell Biology 2019Quote: ... cells that were split a day before were infected with the viral supernatants obtained from the above transfected cells and supplemented with 8 µg of polybrene/ml (Sigma) for at least 6 hours ...
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bioRxiv - Biochemistry 2019Quote: ... The virus-containing media was passed through a 0.45 μm filter and added to U937 cells in media containing 8 μg/mL polybrene (Sigma Aldrich). The cells were spun at 1000 x g for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: Cre expression and activity was induced in 6-8 week old Krt19-CreERT and Rosa26-CreERT mice by intraperitoneal injection of tamoxifen (Sigma) dissolved in corn oil (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... Supernatant was collected at 48 and 72 hours and applied to target cells with 8 μg/mL polybrene (EMD Millipore) for transduction ...
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bioRxiv - Immunology 2019Quote: ... Infection of J774A.1 cells with lentiviruses was performed at 50% confluency in a 10 cm-dish with 8 μg polybrene (Sigma) in the absence of FBS ...
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bioRxiv - Microbiology 2019Quote: ... equal amounts of newly harvested microconidia grown on V8 agar plates for 8 days were cultivated in 0.2x potato dextrose broth (PDB) (Sigma-Aldrich) for 5.5 h and 6.5 h with gentle shaking ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated by loading whole cell lysates onto an 8%-10% SDS-PAGE gel for electrophoresis and then transferred to a PVDF membrane (Immobilon-P, Millipore). Membranes were incubated with primary antibodies in TBST (TBS ...
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bioRxiv - Neuroscience 2019Quote: ... in 0.1M PBS and then developed with a 8 min incubation in DAB-Nickel solution: 10mg 3,3′-diaminobenzidine (DAB) tablet (Sigma, DF905), 0.5 ml of a 2% aqueous nickel sulfate solution ...
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bioRxiv - Cell Biology 2019Quote: ... The pellet was lysed in lysis buffer (50 mM Tris-HCl, pH 8, 125 mM NaCl, 1% NP-40, 2 mM EDTA, 1 mM PMSF [Sigma, 93482-50ML-F] ...
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bioRxiv - Cancer Biology 2019Quote: ... the viral supernatant was collected and added to cells in six-well plates with medium containing 8 μg/ml polybrene (Sigma). After 2 days’ infection ...
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bioRxiv - Immunology 2019Quote: ... They were further diluted 1 in 8 in HBSPE running buffer containing 1% (w/v) carboxymethyl-dextran sodium salt (Sigma) and analysed in the Biacore 3000 instrument at a temperature of 25°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Supernatant containing lentiviral particles was used to infect cancer cells in the presence of 8 μg/ml polybrene (Sigma-Aldrich) overnight ...
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bioRxiv - Cell Biology 2019Quote: ... The virus-containing medium was then used to infect RAWs in the presence of 8 μg/mL polybrene (Sigma-Aldrich) for 24 h ...
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bioRxiv - Immunology 2021Quote: ... the semi-dry protein pellet was dissolved in 25µL of 8 M urea in 20mM EPPS (pH 8.5) (Sigma; Cat#E9502) and was then diluted with EPPS buffer to reduce urea concentration to 4 M ...
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bioRxiv - Neuroscience 2020Quote: ... we prepared solutions of 8 mg/mL antibody in borate buffer (100 mM borate, 2 mM EDTA, pH 8.0; all Sigma-Aldrich). The antibody concentrations were determined from the absorbance at 280 nm (NanoDrop 2000c spectrophotometer ...
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bioRxiv - Microbiology 2021Quote: ... Virus (genome equivalent of a MOI of 1) was added in the presence of 8 μg/ml polybrene (Sigma Aldrich) and the cells were incubated on ice for 1⍰ h to allow virus binding ...
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bioRxiv - Molecular Biology 2021Quote: Type 1 diabetes was induced in male Sprague Dawley rats at 8 weeks of age via a single 55mg/kg tail vein injection of streptozotocin (STZ; Sigma), and monitored for 8 weeks ...
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bioRxiv - Biochemistry 2021Quote: A431 cell pellets were dispersed in lysis buffer (Tris 50 mM pH = 8, 150 mM NaCl, 1% IGEPAL + protease inhibitor cocktail P8340 from Sigma) and incubated on ice with occasional vortexing for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were transduced with the appropriate lentivirus stock for 48h in the presence of 8 μg/mL of polybrene (Millipore) prior to selection with puromycin.
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bioRxiv - Cell Biology 2021Quote: ... Cells were seeded into 6-well plates to reach 50-60% confluency on the day of infection and were transduced 2-3 consecutive days with the viral supernatant in the presence of 8 μg/mL polybrene (Sigma). The viral supernatant was replaced with fresh culture medium ...
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bioRxiv - Cell Biology 2021Quote: ... were equilibrated in 500 μl of assay buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in 500 μl of lysis buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Cell Biology 2021Quote: ... the HEK293T cell lysates transiently expressing mScarlet-I-tagged spartin truncation mutants were lysed in 500 μl of lysis buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1 mM EDTA, 1% TritonX-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Cell Biology 2021Quote: ... 8-chamber coverglass slides (155411, ThermoScientific) were coated with 1 mg/ml of lectin from glycine max soybean (L1395, Sigma) and left for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... The immunoprecipitates were applied to 8 % SDS-PAGE and the gel was stained by ProteoSilver™ stain kit (Sigma-Aldrich) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... Viruses were titrated by infecting THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 μg/ml, Sigma) and incubating for 48 h ...
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bioRxiv - Microbiology 2020Quote: Cell lysate was prepared using lysis buffer (50mM Tris, 150mM NaCl, 1% Triton, pH=8) supplemented with Phosphatase Inhibitor Cocktail (Sigma) at a concentration of 4×106 cells in 100 μL of buffer ...
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bioRxiv - Systems Biology 2020Quote: ... were used for binding assays with a constant amount of fluorescein isothiocyanate (FITC)-conjugated peptides (resuspended in 17 mM Tris-HCl pH 8, 100 mM NaCl and 0.5% Brij L23 (Millipore Sigma), peptides final concentration of 10 nM) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293FT-rKSHV cells were generated by infecting HEK293FT cells with concentrated rKSHV.219 virus (43) in the presence of 8 µg/mL polybrene (Sigma) and by spinoculation (800 g for 30 min at room temperature) ...
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bioRxiv - Microbiology 2021Quote: GFP-LC3 HeLa cells or HeLa-Difluo hLC3 cells were seeded onto Nunc Lab-Tek Chamber Slide system 8 wells (Sigma). The day of the experiment ...
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bioRxiv - Neuroscience 2020Quote: ... Synaptic varicosities were labeled with primary antibody for vesicular glutamate transporter 1 (vGluT1; 1:2000, 8 days incubation, Guinea Pig, MilliPore Biosciece Research Reagent ...
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bioRxiv - Microbiology 2021Quote: ... were transduced with the sgRNA library lentivirus at 0.3 infectious units/cell diluted in 8 μg/ml polybrene (EMD Millipore) as previously described [36] ...
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bioRxiv - Microbiology 2019Quote: ... or PMA-treated THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 µg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD ...
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bioRxiv - Cancer Biology 2021Quote: ... Kelly human NB cells were transduced with the viral supernatant in the presence of 8 μg/ml polybrene (Sigma-Aldrich) and 24 h later selected using puromycin (1 µg/ml ...