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Citations for Millipore Sigma :
1651 - 1700 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The Acetyl CoA content was then determined using the Acetyl coenzyme A assay kit as per the instructions of the manufacturer (Sigma-Aldrich). An Acetyl-CoA standard curve was plotted in the range of 0–100 pmol ...
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bioRxiv - Neuroscience 2023Quote: ... The ATP level for each sample was normalized to the protein content of each sample using the BCA protein assay kit (Sigma-Aldrich). Results are expressed as fold change ± S.E.M ...
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bioRxiv - Molecular Biology 2024Quote: The proximity ligation assay (PLA) was conducted to determine potential protein–protein interactions using the Duolink in situ detection kit (Sigma-Aldrich) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Thiol concentration of culture supernatants was measured using a fluorometric thiol assay kit according to the manufacturer’s instructions (Sigma-Aldrich, MAK151). Fluorescence was measured with a BioTek Synergy H1 microplate reader (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded in 96-well plates (2 × 103 cells/well) and analyzed with the MTT Cell Growth Assay Kit II (Sigma-Aldrich) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay in imaginal wing discs was performed using the ApopTag red in situ apoptosis detection Kit (#S7165, Sigma-Aldrich), following the manufacturer’s procedure ...
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bioRxiv - Cancer Biology 2024Quote: The interaction between CAMKV and CREB was assessed in SK-N-AS cells using an in situ PLA assay (Duolink in situ red starter kit, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Routine genotype analysis of mice was performed by PCR assay on DNA purified from tail biopsies (Extract N-Amp Tissue PCR kit, Sigma Aldrich). Wild-type and KO mutant alleles were distinguished using primers available on request ...
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bioRxiv - Pathology 2023Quote: Serum iron and TIBC were determined using the Total Iron-Binding Capacity and Serum Iron Assay Kit (MAK394, Sigma Aldrich, USA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and Aβ 1-42 using the Milliplex® MAP Human Amyloid Beta and Tau Multiplex Assay kit (Millipore Sigma HNABTMAG-68K). All kits were read on a MAGPIX® system (Luminex ...
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bioRxiv - Physiology 2023Quote: ... Peroxidase-Glucose oxidase (PGO) enzyme plus color reagent was added to each well (Glucose Assay Kit: Sigma GAG020, PGO Sigma P7119), the plate was incubated at 37°C and the optic density was read at 450 nm using a Tecan Spark Microplate Reader ...
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bioRxiv - Physiology 2023Quote: ... Peroxidase-Glucose oxidase (PGO) enzyme plus color reagent was added to each well (Glucose Assay Kit: Sigma GAG020, PGO Sigma P7119), the plate was incubated at 37°C and the optic density was read at 450 nm using a Tecan Spark Microplate Reader ...
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bioRxiv - Immunology 2023Quote: Inflammation-associated with neutrophils and monocytes tissue infiltration was indirectly determined by measuring the MPO activity in 2 cm length fragments of distal colon using an MPO colorimetric activity kit assay (Sigma-Aldrich), following the manufactures instructions.
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bioRxiv - Biochemistry 2023Quote: ... One half was used for protein extraction and the other half used for UA quantification using a UA Assay Kit (MAK077, Sigma-Aldrich). Cells were disrupted mechanically ...
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bioRxiv - Biochemistry 2023Quote: Duolink proximity ligation assays were performed using a Duolink™ In Situ PLA Kit Goat/Rabbit (Olink Biosciences) purchased from Sigma. Briefly ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... 4-octanol used in the GC analysis and amylase activity assay kit were purchased from Sigma-Aldrich (Castle Hill NSW, Australia). Micro bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2023Quote: ... samples were aspirated from each well and kept on ice for analysis with a colorimetric glutamate assay kit (Sigma-Aldrich; MAK004), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: In situ proximity ligation assays (PLAs) were performed using Duolink in situ red starter kit according to the manufacturer’s instructions (Millipore Sigma, DUO92101). Briefly ...
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bioRxiv - Biophysics 2023Quote: The ATP hydrolysis activity of ChlI was assessed by quantification of free orthophosphate using the malachite green phosphate assay kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Serum samples were collected from surviving mice and 3 samples from each group were used for estimation of Creatine Kinase activity Assay Kit (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2023Quote: TUNEL assays were performed after secondary antibody staining by using the In Situ Cell Death Detection Kit TMR red (Millipore #12156792910). Embryos were incubated for 4 hours at room temperature (RT ...
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bioRxiv - Cancer Biology 2023Quote: ... To evaluate SDH activity cells were stimulated as described above and SDH activity was analyzed with Succinate dehydrogenase Activity Colorimetric Assay Kit (Sigma-Aldrich) according to the manufacturers′ instructions.
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bioRxiv - Biochemistry 2023Quote: Free and encapsulated DyP peroxidase (KpDyP and KpDyP_Enc) were purified and diluted to equimolar heme concentrations as determined via heme assay kit (Sigma-Aldrich, USA) per manufacturer protocol to analyze enzyme activity via ABTS assays ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein quantification was performed using the BCA protein quantification assay (QPro BCA kit, Cyanagen) against a bovine serum albumin (BSA) calibration curve (Sigma Aldrich).
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bioRxiv - Cancer Biology 2023Quote: ... the lyophilized powder was reconstituted with deionized (DI) water and processed using the Sigma Glutamate Assay Kit (catalog no: MAK330, Sigma Aldrich) while the protein content was measured using the Pierce™ BCA Protein Assay Kit (catalog no ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue triglyceride assays were performed as previously described using a Triglyceride Quantification Colorimetric/Fluorimetric Kit (Cat. no. MAK266 Sigma-Aldrich, Inc.) (23).
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bioRxiv - Cancer Biology 2023Quote: ... The p-nitrocathecol standard was prepared according to the manufacturer’s protocol for the sulfatase activity assay kit (Sigma, Cat# MAK276-1KT). Absorbance readings at 515 nm were obtained from a Bioteck Synergy HT plate reader ...
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bioRxiv - Bioengineering 2023Quote: ... the samples were first homogenized and hydrolyzed in concentrated hydrochloric acid (HCl) at 120°C for 3 hours before being quantified using hydroxyproline assay kit (Sigma-Aldrich). A microplate reader (Promega GloMax ...
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bioRxiv - Cell Biology 2024Quote: The interaction between PRDX6 and SEPHS2 in SK-N-DZ WT and SK-N-DZ PRDX6 KO cells was observed using Duolink in situ proximity ligation assay (PLA) kit (DUO92101, Sigma- Aldrich) in the presence or absence of 600nm Selenocysteine ...
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bioRxiv - Plant Biology 2024Quote: ... To quantify the concentration of purified protein in the samples a Bicinchoninic acid (BCA) assay was conducted using the BCA kit following the manufacturer recommendations (Sigma Aldrich).
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded in VitroGel at 50% confluence on chamber slides for PLA (Proximity Ligation Assay) using the Duolink in situ Detection Reagents Red kit (DUO92008, Sigma-Aldrich), following the given protocol ...
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bioRxiv - Microbiology 2024Quote: Cytotoxicity was studied according to the manufacturer’s instruction either using cytotoxicity LDH assay kit-WST (Dojindo) or by measuring transepithelial electrical resistance (TEER) (Millicell® ERS-2, Millipore) as previously described (25) ...
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bioRxiv - Genetics 2024Quote: ... The ADP/ATP ratio was quantitatively measured from lysis supernatant using the Sigma ADP/ATP Assay Kit (Sigma-Aldrich, MAK135-1KT), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Luminex assays detecting soluble ICAM-1 (sICAM1) were conducted using the MILLIPLEX Human Sepsis Magnetic Bead Panel 1 Kit (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... the level of ketone bodies (β-hydroxybutyric acid) was determined using a Sigma-Aldrich Ketone Body Assay Kit (Sigma-Aldrich, MAK134). To indicate possible insulin-resistance among obese mice the level of non-esterified fatty acids was measured using an ELISA kit (Sigma-Aldrich ...
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bioRxiv - Pathology 2024Quote: NAD and NADH concentrations in H9C2 cells were quantified using the NAD/NADH Assay Kit according to the manufacturer’s protocol (Sigma-Aldrich, MAK468). Optical density was measured using a BMG Labtech Spectrostar NanoDosage.
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bioRxiv - Pathology 2024Quote: Succinate concentrations in H9C2 cells were quantified using the Succinate Colorimetric Assay Kit according to the manufacturer’s protocol (Sigma-Aldrich, MAK184). The colorimetric measurements were taken using a TECAN 500 microplate reader.
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bioRxiv - Cancer Biology 2024Quote: ... the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in serum samples using Alanine Aminotransferase Activity Assay Kit (MAK052, Sigma-Aldrich) and Aspartate Aminotransferase Activity Assay Kit (MAK055 ...
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bioRxiv - Cell Biology 2024Quote: The quantification of hydroxyproline content in mouse lungs was conducted using a Hydroxyproline Assay Kit (MAK008, Sigma, St. Louis, MO, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: Total protein extract from the different plants was separated on 10% SDS-PAGE gel and transferred to a PVDF membrane (Immobilon-P Transfer membrane, Millipore) by semi-dry blotting in 25 mM Tris–HCl ...
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bioRxiv - Cell Biology 2022Quote: ... Nonspecific binding sites on the membrane were blocked by incubating the membrane in 5% milk powder in PBS containing 1X Tween-20 (Sigma). The membrane was then incubated with the primary antibody of interest at 40C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... and the electrophoresed proteins were then transferred onto a polyvinylidene difluoride membrane (Immobilon®-PSQ membrane, Millipore GmbH, Eschborn, Germany) for immunoblotting analysis ...
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bioRxiv - Neuroscience 2022Quote: ... dissected hippocampi were cut into 400 μm pieces with a tissue chopper and placed on a porous membrane (2 slices per membrane, Millicell CM, Millipore) in six-well plates ...
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bioRxiv - Neuroscience 2022Quote: ... from CNO and saline treated mice were first boiled at 95°C for 5 min and then separated on 10% polyacrylamide gel and transferred on PVDF membranes (Immobilon-P transfer membrane; Merck Millipore). Membranes were then blocked with 5% non-fat powdered milk in TBS + 0.01% Tween-20 (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... and filtrated using membrane filters with a pore size of 0.45 μm (Millex® HV membrane filter units, Merck Millipore). The clarified lysate was supplemented with Ni-NTA agarose (1.3 ml resin/ 1 l insect cell pellet ...
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bioRxiv - Developmental Biology 2022Quote: ... separated on 15% sodium dodecyl sulfate-polyacrylamide electrophoresis gels and transferred to a polyvinylidene difluoride membrane (Immobilon-P Transfer membrane, Millipore) by wet blotting in transfer buffer (25 mM Tris– HCl ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μg of protein per sample was loaded and separated on 8-12% SDS–PAGE gels and electro blotted onto polyvinylidenedifluoride (PVDF) membranes (Immobilon-P PVDF membrane 0.45μm; Merck-Millipore, Burlington, USA). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... and subsequently filtrated using membrane filters with a pore size of 0.45 μm (Millex® HV membrane filter units, Merck Millipore). The clarified lysate was supplemented with Glutathione Sepharose 4B (Cytiva ...
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bioRxiv - Cancer Biology 2024Quote: ... Total cellular lysates were separated on SDS/PAGE gels and transferred to PVDF membrane (Immobilon®-PSQ Transfer membrane, Millipore). The membranes were blocked at RT with 10% milk in TBS with 0.5% tween-20 for 2 hours followed by overnight incubation with primary antibodies at 4°C with gentle agitation.
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bioRxiv - Biochemistry 2023Quote: ... N-glycomics of BLCA cell lines was performed similarly after immobilization of membrane protein extracts in a hydrophobic PVDF membrane (MAIPS4510; Millipore). All glycans were permethylated and analysed by reverse phase nanoLC- ESI-MS/MS as previously described by us69 ...