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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... before being cut into 250μm parasagittal slices using a McIlwain tissue chopper and placed on Millicell membrane (3 to 4 slices each per animal, 0.4 μm membranes, Merck Millipore) in 50% BME (41010026 ...
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bioRxiv - Biophysics 2024Quote: ... spread on a conjugation membrane (mixed cellulose ester membrane from Millipore, 47 mm diameter, and 0.45 μm pore size) over an LB+agarose+DAP+Ara Petri dish and incubated 3 hours for conjugation and recombination to take place ...
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bioRxiv - Molecular Biology 2024Quote: SDS-PAGE (10%) was followed by a semidry blotting method using PVDF membrane (Millipore Immobilon-P membrane, 0.45 µm). For blocking 5 (m/v ...
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bioRxiv - Biophysics 2024Quote: ... The resulting product was then dialysed for 3h by using a membrane filter (MF-Millipore 0.025 µm membrane, Merck) and concentrated 10X using a speed vacuum concentrator ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were then transferred onto methanol-activated polyvinylidene fluoride (PVDF) membrane (Immobilon-FL membrane, pore size 0.45μm; Millipore, IPFL00010). Membrane was blocked ...
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bioRxiv - Cell Biology 2024Quote: ... and to Geltrex-coated filter membrane inserts (pore size 1.0 μm PET membrane for 6-well plate Millicell PLRP06H48, Millipore) for isolation of RNA or protein from subcellular compartments or imaging on filters ...
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bioRxiv - Evolutionary Biology 2021Quote: ... triglyceride assays (Pointe Scientific T7532-120, T7531-STD) and soluble protein assays (Sigma B9643-1L) were then carried out in triplicate according to the kit instructions ...
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bioRxiv - Cancer Biology 2022Quote: Proximity ligation assay was performed with Duolink® Proximity Ligation Assay (Sigma Aldrich-Merck, DUO92102) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... donor cells were labelled with CellVue Claret Far Red Fluorescent Cell Linker Mini Kit for General Membrane Labelling (Sigma Aldrich, Cat#MINCLARET-1KT) following manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... ROS release was monitored for three hours with measurements every two minutes using a fluorometric hydrogen peroxide assay kit (Sigma-Aldrich) and a Glomax microplate reader (Promega ...
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bioRxiv - Immunology 2021Quote: ... Liver triglyceride and total cholesterol concentrations were measured using commercial kits (all from Instruchemie) and expressed as nanomoles per milligram of total protein content using the Bradford protein assay kit (Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... were obtained using terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay (82) (based on in Situ Cell Death Detection Kit, 11684795910, red fluorescence; Sigma-Aldrich) on sections derived from rd1 and C3H retinal explant cultures ...
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bioRxiv - Cell Biology 2021Quote: ... proximity ligation assays were performed on isolated HSCs with the Duolink in Situ Red Starter Kit Mouse/Rabbit (DUO92101, Millipore Sigma), adapting the vendor’s protocol for HSCs ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and cortex was determined following the standard Ellman reaction principle using a commercially available AChE activity assay kit (Catalog no. MAK119, Sigma-Millipore). The Ellman assay [42] was used to measure ChE activity in each brain region using 5,5’-dithio-bis (2-nitrobenzoic acid ...
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bioRxiv - Plant Biology 2021Quote: The inhibitory effect of recombinant P235 on recombinant glycosyl hydrolase was assayed using the β-Glucosidase Activity Assay Kit (MAK129, Sigma). Enzymatic reactions were carried out in K-Phosphate buffer (100 mM ...
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bioRxiv - Bioengineering 2021Quote: ... 250 μL of recovered solution was transferred into a 96-well plate and the recovered glucose concentration was measured using a glucose (GO) assay kit purchased from Sigma (GAGO20). Glucose recovery rate was defined by the following formula.
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bioRxiv - Cell Biology 2020Quote: ... 30 μL of samples and standards were added to a 96-well plate and glucose was measured using the Glucose Oxidase (GO) Assay kits (Sigma, GAGO20). Total circulating sugars was measured from the trehalase-treated sample ...
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bioRxiv - Cell Biology 2021Quote: ... The TUNEL assay was performed on the testis sections (5 μm) with ApopTag plus peroxidase in situ apoptosis detection kit (Millipore, S7101).
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed with 2% paraformaldehyde in PBS and proximity ligation assays (PLA) were performed with the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2021Quote: CoA levels in the protein solution after proteinase K digestion were quantified using a commercial CoA assay kit according to manufacturer’s protocol (Sigma-Aldrich; MAK034). CoA concentration is determined by an enzymatic assay ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained for proximity ligation assay (PLA) using a Duolink® kit according to the manufacturer’s protocol (DUO92101, Millipore Sigma). Briefly ...
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bioRxiv - Physiology 2022Quote: Determination of cellular total iron levels in magnetically-sorted RPMs was carried out using the Iron assay Kit (Sigma-Aldrich, MAK025) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The measurement of total glutathione (tGSH) was determined (in pellets and supernatants) using the kinetic glutathione assay kit (Sigma-Aldrich, Spain) in which catalytic amounts (nmoles ...
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bioRxiv - Cell Biology 2022Quote: To quantify extracellular levels of lactate and pyruvate from iHep supernatants aliquots were taken at day 22 of differentiation and analyzed using Lactate and Pyruvate Assay Kits (Sigma-Aldrich) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: RAS and RAC activity were measured using a RAF-1 RBD and PAK-1 PDB pull-down assay kits respectively (Cat#17218 and Cat#14325, Millipore Sigma) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Levels of nitrate and nitrite in plasma obtained from human and mice were estimated by a Griess colorimetric assay kit (Sigma chemicals) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The precipitated proteins were used for determination of protein content using a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich, Taufkirchen, Germany).
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bioRxiv - Microbiology 2021Quote: Cell viability and cytotoxicity of antibiotics and peptides were determined against human corneal epithelial cells (HCE-2, CRL-11135, ATCC, Manassas, Virginia, USA) using cell-counting-kit-8 (CCK-8) assay (Sigma Aldrich, Merck Life Science UK Limited ...
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bioRxiv - Microbiology 2020Quote: Protein concentration was determined by the bicinchoninic acid protein assay kit (BCA-Pierce, USA) using bovine serum albumin (BSA, Sigma-Aldrich) as standard.
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bioRxiv - Microbiology 2021Quote: ... samples were centrifuged at 10,000g for 5 min and the Acetyl-CoA measurement was performed in a 96-well plate using the Acetyl-Coenzyme A Assay Kit (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Lactate dehydrogenase (LDH)-based cytotoxicity levels were determined by evaluation of LDH release into the cell culture supernatants using Lactate Dehydrogenase Activity Assay kit (MAK066, Sigma-Aldrich). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... catalysis (ChE activity) were determined either as previously described (Hagstrom et al. 2017) or using an Acetylcholinesterase Activity Assay Kit (Sigma-Aldrich). Absorbance was read at 412 nm every minute for 10 minutes using a VersaMax (Molecular Devices ...
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bioRxiv - Genetics 2022Quote: ... The external ammonium concentration was estimated as a function of time for 3 hours using the below-mentioned principle (Ammonia Assay Kit - Sigma-Aldrich). At each time point ...
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bioRxiv - Molecular Biology 2022Quote: ... Secretion of the following cytokines was quantified with the MILLIPLEX® Human CD8+ T Cell Magnetic Bead Panel Premixed 17 Plex - Immunology Multiplex Assay kit (EMD-Millipore): IL-6 ...
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bioRxiv - Pathology 2020Quote: ... 120 or 180 min after the blood collection) was addressed spectrophotometrically using Lactate Dehydrogenase Activity Assay Kit (MAK066, Sigma Aldrich, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: Viability of the slices was assessed by measuring the ATP content per milligram tissue using a bioluminescence assay kit (Sigma-Aldrich) as previously reported by Hadi et al ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was collected and used immediately in a commercial urease activity assay kit (Sigma-Aldrich MAK120, St. Louis, MO, USA).
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bioRxiv - Microbiology 2021Quote: In situ proximity ligation assay (PLA) was performed using the Duolink™ In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Physiology 2021Quote: The BHB concentration was quantified using the Beta-Hydroxybutyrate Assay Kit (MAK041) according to the technical bulletin (Sigma-Aldrich, United States). The concentration of BHB was expressed in ng/μg protein.
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bioRxiv - Microbiology 2021Quote: Proximity ligation assay (PLA) was performed using the Duolink In Situ Red Started Kit Mouse/Rabbit (Millipore Sigma, Cat# DUO92101-1KT) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: GlgE activity was monitored quantitatively in the elongation direction by the release of orthophosphate using the Malachite Green Assay Kit (Sigma-Aldrich) following the manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: Phosphatase and kinase activities of SiaA and/or SiaB on SiaC and/or SiaCp were determined using the Malachite green phosphate assay kit (Sigma Aldrich) and ADP-Glo™ assay kit (Promega ...
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Necrosis reduction efficacy of subdermal biomaterial mediated oxygen delivery in ischemic skin flapsbioRxiv - Bioengineering 2022Quote: ... sections of the flap were frozen at - 80°C and processed [24] to measure the lactate content of the tissues using a Lactate assay kit (Sigma-Aldrich).
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bioRxiv - Biochemistry 2022Quote: Activity assays to see the inhibitory effect of Halo Library fragment binders on RT52A activity were performed using the Roche colorimetric assay kit purchased from Sigma Aldrich, using the absorbance measurement of 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid ...
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bioRxiv - Cell Biology 2022Quote: Fresh-frozen sections of young and aged bladders were tested for acid phosphatase activity using an acid phosphatase assay kit (CS0740-1KT, Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: The activity of the MtPAPP was determined by the production of orthophosphate which was quantified using the Malachite Green Phosphate Assay Kit (Sigma-Aldrich) by forming a green complex ...
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bioRxiv - Microbiology 2022Quote: ... 12 and 16 hours of bacterium growth for 24 hours at 37°C with shaking (180 rpm) using the Iron Assay Kit (Sigma-Aldrich). TECAN infinite M200PRO microplate reader was used for measuring Colorimetric readings.
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bioRxiv - Plant Biology 2022Quote: ATP levels in media from hydroponic infection experiments of hydroponically-grown plants were analyzed using the ATP Colorimetric/Fluorometric Assay Kit (Sigma, USA) and an Infinite M1000 plate reader (Tecan ...
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bioRxiv - Molecular Biology 2023Quote: ... Following primary antibody incubation coverslips were washed 2× in PBS and then the proximity ligation assay was carried out (Duolink In Situ Red Starter kit (Sigma Aldrich) according to the manufacturer’s instructions) ...
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bioRxiv - Molecular Biology 2022Quote: CTCF Chromatin immunoprecipitation (ChIP) was performed on 1 x 107 Ter119+ erythroid cells using a ChIP Assay Kit (Millipore: 17-295) according to the manufacturer’s instructions ...