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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Proximity ligation assay (PLA (53)) was performed using the In Situ Red Starter Kit Mouse/Rabbit (Duolink DUO92101, Sigma Aldrich). The conditions specified by the manufacturer’s instructions were optimized by incubating sections with primary antibodies overnight at 4℃ and increasing incubation time with probes and amplification reagents to 2 hours as previously described (54) ...
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bioRxiv - Immunology 2022Quote: ... an aliquot of the sample was taken for measuring the total protein concentration using a BCA assay kit (Merck Millipore). The remaining sample was heat-treated (70 °C ...
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bioRxiv - Immunology 2022Quote: ... Adenosine 5′-triphosphate (ATP) was assessed in the cell-free TA supernatants using bioluminescent assay kit following the manufacturer’s instructions (Sigma-Aldrich). The levels of soluble immune mediators were measured in TA samples using a high-throughput microbeads array (Bio-Plex Pro™ Human Cytokine 27-plex Assay ...
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bioRxiv - Microbiology 2022Quote: ... The effect of GpsB on the GTPase activity of FtsZ and FtsZΔC6 was determined by measuring the free phosphate released using the malachite green phosphate assay kit (Sigma Aldrich). Briefly ...
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bioRxiv - Physiology 2023Quote: ... a colorimetric-based enzyme assay was used to measure alanine aminotransferase (ALT) levels in peripheral plasma collected from the submandibular vein monthly using a Mouse ALT Activity Assay Kit (SIGMA). Serum LBP level was measured by a Mouse Lipopolysaccharide Binding Protein ELISA Kit (HyCult Biotechnology ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were further treated in a 96-well plate following the “Coenzyme A Assay Kit” protocol from Sigma-Aldrich. The absorbance of the colorimetric product (570 nm ...
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bioRxiv - Bioengineering 2023Quote: ... The phosphate concentration was then measured according to the manufacturer’s instructions (Malachite Green Phosphate Assay Kit, Sigma-Aldrich, The Netherlands). Briefly ...
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bioRxiv - Cell Biology 2023Quote: The proximal ligation assay (PLA) was performed using the Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92103; Sigma-Aldrich), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The total ATP production was measure in nm/µg of protein by the ATP colorimetric assay kit (Sigma, MAK190-1KT) and experiment was performed as per provided manufacturer’s procedure ...
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bioRxiv - Neuroscience 2024Quote: ... Levels of individual cytokines were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) and involving the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
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bioRxiv - Cell Biology 2024Quote: ... The amount of sialic acid in the liposomes was determined using a Sialic Acid Assay Kit (MAK314-1KT, Sigma Aldrich), and the concentration of liposomes containing a ganglioside was adjusted to the same level for use in microscopic observation.
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bioRxiv - Molecular Biology 2024Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed with paraffin embedded sections using a TUNEL assay kit following the manufacturer’s instructions (Millipore, S7110). For DHE staining ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Protein activity assays were conducted using 10 μL of sample (3x replication) on a Chitinase Activity Kit (Sigma-Aldrich #CS0980) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of hydrogen peroxide in the supernatants was determined using the Fluorimetric Hydrogen Peroxide Assay Kit (Sigma-Aldrich, MAK166) following the supplier’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... in primary neuronal lysate after Aβ or gAβ treatment from mouse or human brain were quantified by the Glycerol-3-Phosphate Assay Kit (Sigma), High Sensitivity DHAP Fluorometric Assay Kit (Sigma) ...
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bioRxiv - Microbiology 2024Quote: The effect of GpsB on the inorganic phosphate released by FtsZ and truncated FtsZ was examined using malachite green phosphate assay kit (Sigma). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein content of the supernatant was estimated with QuantiPro™ BCA Assay kit (Sigma Aldrich, Saint-Quentin-Fallavier, France) before adding 5X Laemmli + 5 % β- mercaptoethanol and heating at 95 °C for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... A standard curve for the MDA Assay was generated and MDA was measured using a kit in accordance with the manufacturer’s instructions (SIGMA-ALDRICH Lipid Peroxidation malondialdehyde (MDA ...
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bioRxiv - Biophysics 2024Quote: ATPase activities for the designed proteins were measured by the ATPase/GTPase Activity Assay Kit (Sigma-Aldrich Co. LLC, MAK113). Protein samples at concentrations of 25 μM (for reactions at 45 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration of D-2HG in the cell lysates was determined using the D-2-Hydroxyglutarate (D-2HG) Assay Kit (Ref MAK320, Sigma) and LC/MS for validation ...
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bioRxiv - Bioengineering 2024Quote: Cell viability on the mouse hindlimb culture was assessed on days 1 and 7 using the XTT cell viability assay kit (Sigma Millipore ...
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bioRxiv - Cell Biology 2024Quote: Enzyme activity of glutamic-oxaloacetic transaminase (GOT) and pyruvate dehydrogenase (PDH) was measured in BAT tissue homogenates by the GOT and PDH Activity Assay kits (Sigma).
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bioRxiv - Cell Biology 2024Quote: Intracellular ATP/ADP ratio was measured using a luminescence detection using ADP/ATP ratio assay kit (Sigma, Cat. No. MAK135), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The remaining homogenate was frozen and kept for subsequent protein content analysis using a QuantiPro BCA Assay Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: Duolink proximity ligation assay (Sigma) was carried out largely in congruence with manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: The EdU Click assay (Sigma) was used to detect proliferating cells and carry out lineage tracing in vivo ...
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bioRxiv - Cell Biology 2022Quote: BCA protein assay (Sigma-Aldrich) was performed to measure protein concentrations ...
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bioRxiv - Cancer Biology 2021Quote: Radioimmunoprecipitation assay (RIPA) buffers (Millipore) containing protease (Roche ...
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bioRxiv - Pathology 2021Quote: ... according to Bradford assay (Sigma) to 80 ng/μl with LDS sample buffer (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... The PLA assay (Sigma Aldrich) was then performed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Radioimmunoprecipitation assay (RIPA) buffer (Millipore) supplemented with protease inhibitor cocktail (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... The PicoProbeTM assay (Sigma Millipore) and analysis were performed according to the manufacturer’s protocols using the picomole standard curve (n = 1) ...
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bioRxiv - Biochemistry 2024Quote: ... The PicoProbeTM assay (Sigma Millipore) and analysis were performed according to the manufacturer’s protocols using the picomole standard curve (n = 1) ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... For MTT assays (Millipore; Merck), MTT reagent was added for 4 h and absorbance measured as 570 nm (cell absorbance ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... For CCK8 assays (Sigma Aldrich), CCK8 reagent was added for 2 h and absorbance measured at 450 nm on a Spectramax® 190 Microplate Reader (Molecular Devices ...
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bioRxiv - Microbiology 2023Quote: Phenylalanine assay (Sigma-Aldrich MAK005) utilizes a coupled enzyme assay that results in the deamination of phenylalanine and the production of NADH which reacts with the probe resulting in a fluorescent (λex = 535 nm/λem = 587 nm ...
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bioRxiv - Immunology 2024Quote: ... we used Bradford assay (Sigma).
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bioRxiv - Physiology 2023Quote: ... Hydroxyproline assay (Sigma Aldrich, MAK008) was used to quantify total collagen content in liver and adipose tissues according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The Duolink Proximity Assay (Sigma) was executed as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... DuoLink® assay (Sigma-Aldrich) was performed according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Immunology 2024Quote: ChIP assay (Millipore, 17-295) was performed according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: α-synuclein was fixed to membranes by boiling for 10 minutes in PBS before membranes were washed in 0.05 % Tween 20 (Sigma) containing Tris-buffered saline (TBST ...
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bioRxiv - Bioengineering 2021Quote: Membranes used for the microporous membrane scaffold method were 0.45 µM pore size and 47 mm diameter (Sigma-Aldrich). Prior to sampling ...
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bioRxiv - Cell Biology 2021Quote: ... by mixing ~1×107 log-phase cells of each strain on a filter membrane (0.8 μm MCE Membrane Filter, MF-Millipore) on top of a YPD plate for 5 hours at 30 °C ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were diluted to a final concentration of 1 × 106 CFU/mL in sterile 1X PBS and were stained in the dark for membrane potentials using 30 μM membrane potential indicator dye DIO2(3) (3,3=-diethyloxacarbocyanine iodide) (320684; Sigma) for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1 h at 50 V followed by 3.5 h at 100 V and blotted on PVDF-membranes (Immobilon-PSQ Membrane, Merck Millipore). Incorporation of [35S]-methionine into newly synthesized proteins enabled the detection of translation products by phosphor imaging (exposure time of 1 day).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... for 72h on porous membranes (Whatman ® Nucleopore™ Track-Etched Membranes WHA150446, Sigma Aldrich, St. Quentin Fallavier, France) in 4-well plates [21] ...
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bioRxiv - Plant Biology 2022Quote: ... Either total membrane or immunoprecipitated proteins were separated on a SDS-PAGE gel and transferred to PDVF membrane (Millipore) for immunoblot analysis using monoclonal anti-GFP (33-2600 ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... followed by transfer onto methanol-activated polyvinylidine fluoride (PVDF) membrane (Immobilon-FL membrane, pore size 0.45 μm; Millipore, IPFL00010). Membrane was blocked ...
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bioRxiv - Neuroscience 2024Quote: ... before being cut into 250μm parasagittal slices using a McIlwain tissue chopper and placed on Millicell membrane (3 to 4 slices each per animal, 0.4 μm membranes, Merck Millipore) in 50% BME (41010026 ...