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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for 2' Methyl 3 phenylpropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Dissected tumors were washed in PBS and homogenized at room temperature in urea lysis buffer (8 M urea, 40 mM Tris pH 7.6, 5% SDS supplemented with phosphatase inhibitor cocktails 2, 3 (Sigma P5726, P0044)) ...
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bioRxiv - Bioengineering 2022Quote: ... HA-TBA was dissolved in anhydrous DMSO (2 wt%) with a 3:1 M ratio of 5-norbornene-2-carboxylic acid (mixture of endo and exo isomers; Millipore-Sigma) to HA-TBA repeat units ...
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bioRxiv - Microbiology 2022Quote: ... Cleavage of the tag was performed in 2-3 ml with 20 μl 3C protease (1 mg/ml, Sigma-Aldrich, SAE0045) added and incubated over-night at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT, 0.5 mg/ml in PBS, Sigma-Aldrich, USA) with 1 μM of menadione (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed 3 times in cold phosphate buffer and postfixed with a solution of 2% osmium tetroxide (#75632 Sigma-Aldrich) and 1.5 % potassium ferrocyanide (P3289 Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% (BC-1, BCBL-1) or 20% (BC-2, BC-3, BC-5, BJAB) Serum Plus-II (Sigma-Aldrich, catalog number 14009C ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue sample was trimmed to 2×3×0.2 mm3 and scanned with a multi-beam serial-section scanning electron microscope (Sigma, Carl Zeiss) in 4×4 nm2 pixels and 33 nm slice thickness ...
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bioRxiv - Microbiology 2024Quote: ... respectively) by dilution in a vehicle solution of 10% w/v 2-hydroxypropyl-!3-cyclodextrin (Sigma-Aldrich, St-Louis, MO, USA) in PBS ...
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bioRxiv - Cancer Biology 2024Quote: Tumours and control tissues (comparable anatomical region) were harvested and dissociated using a hand-held tissue homogenizer (Omni International Inc.) in 2-3 ml RNAzol (Sigma, 4533). Total RNA was isolated using RNAzol ...
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bioRxiv - Cancer Biology 2024Quote: ... were washed two times with PBS and lysed by scraping into 500 μl per plate of lysis buffer (40 mM Tris/HCl pH 7.6, 8 M urea, EDTA-free protease inhibitor complete mini [Roche], and phosphatase inhibitor cocktails 1, 2, and 3 [Sigma-Aldrich] at 1× final concentration according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... were adjusted to 5 x 106 bacteria per 50 μL in PBS then treated with indicated OspC MAbs (1, 2, 3, 10, 20, 30 μg/mL) in the presence or absence of 20% human complement (Sigma-Aldrich) for 16 or 24 h ...
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bioRxiv - Microbiology 2024Quote: ... The remaining RBC pellet was suspended in 2 mL of incomplete media and then gently layered on to a 3 mL aliquot of percoll (Sigma Aldrich) diluted with 1X RPMI (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were then incubated for 2-3 hours at 4°C with either 5 μl of anti-FLAG-M2 affinity resin (Sigma-Aldrich), Streptactin sepharose (IBA Lifesciences) ...
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bioRxiv - Biochemistry 2024Quote: ... 1% Triton X-100] and incubated for 2-3 hours at 4°C with either 5 μl of anti-FLAG-M2 affinity resin (Sigma-Aldrich), Streptactin sepharose (IBA Lifesciences) ...
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bioRxiv - Microbiology 2024Quote: ... Larvae of indeterminate sex were infected by injection of 10nL into the caudal vein at 3 days post fertilization (dpf) using a capillary needle containing bacteria diluted in PBS + 2% phenol red (Sigma #P3532), as previously described [104] ...
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bioRxiv - Immunology 2024Quote: ... a subset of the mice (n = 3-4 per group) received intraperitoneal (i.p.) injections of 2 mg/kg WFA (Millipore-Sigma, #681535), or equivalent volume of the vehicle (VC ...
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bioRxiv - Synthetic Biology 2024Quote: ... All the reagents were prepared in enough amounts for all the experiments for the 2 systems of colicin M and E1 except for the 3-PGA (Sigma Aldrich) was used for colicin M while creatine phosphate (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with TBS-T (TBS + 0.05% Tween20) (3 times x 2 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Plain media was added overnight and on day 3 cells were passaged into selection media containing 2 µg/uL puromycin (Sigma P8833) and 150 µg/mL hygromycin B (Thermo 10687010 ...
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bioRxiv - Cancer Biology 2024Quote: ... livers were washed 3 times with PBS and incubated 1 hour at room temperature with blocking solution (PBS containing 2 % BSA, Sigma Aldrich) followed by incubation at 4 °C overnight with primary antibody staining (blocking solution containing 1:100 primary antibody) ...
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bioRxiv - Cancer Biology 2024Quote: Spheroids grown in collagen for 3/6 days underwent collagen digestion by incubation in DMEM without serum supplemented with 2 mg/ml collagenase (Sigma, C2139) for 45 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Osteogenic differentiation was performed in osteogenic medium for 2 weeks (medium was changed every 3 days) and quantified by alizarin red S staining (Sigma, A5533). The osteogenic differentiation medium contained DMEM supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... After 72 hrs we performed 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays using the Roche MTT protocol (Sigma Aldrich Cat#11465007001). After 24 hours light absorption at 570 nm was measured using the MTT plate reader (Wallac EnVision 1.12) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were allowed to grow for 96 h in drug containing medium (methyl methanesulfonate, Acros Organics 156890250, mitomycin C, Sigma M4287 ...
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bioRxiv - Systems Biology 2020Quote: ... and ddH2O and stained for cell volume with 12.5 μg/ml bis(2,2’-bipyridine)-4’-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophosphate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate ...
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bioRxiv - Microbiology 2021Quote: ... the samples were dissolved in 50 μL of 20 mg/ml methoxyamine hydrochloride in pyridine for 1.5 h at 33°C followed by derivatization with N-Methyl-N-(trymethylsolyl)trifluoroacetamide (MSTFA, Sigma Aldrich) for 2 h at 35°C.
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bioRxiv - Neuroscience 2020Quote: ... The lesions were made by injecting N-Methyl-D-aspartic acid (10 μg/μL NMDA in 0.9% saline; Sigma, Germany) into the dorsoventral and mediolateral hippocampus at 8 sites bilaterally at a flow rate of 0.15 μL/min (see (Table 1 for coordinates) ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... and incubated at 37° C for 90 minutes followed by addition of 20μL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert–Butyldimethylchlorosilane (Sigma 375934) and incubated at 60°C for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated at 37°C for 90 minutes followed by addition of 20µL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert– Butyldimethylchlorosilane (Sigma 375934) and incubated at 60°C for 1 hour ...
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bioRxiv - Plant Biology 2021Quote: ... residual trypsin activity was measured by monitoring the change in absorbance at 247 nm in presence of increasing concentration of recombinant purified Kunitz Trypsin inhibitor (KTI) when incubated with p-toluene-sulfonyl-L-Arg methyl ester (Sigma). To study the inhibitory action of P235 on KTI action ...
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bioRxiv - Biochemistry 2022Quote: ... Blocking Buffer (100 mM HEPES, pH 7.5 [Gibco], 1 mM EDTA [Sigma-Aldrich], 2.5% SDS [Sigma-Aldrich], 0.1% S-methyl methanethiosulfonate [Sigma-Aldrich]), Binding Buffer (100 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... The sample was then derivatized by adding 60 μL N-methyl-N-(trimethylsilyl)trifluoro acetamide (MSTFA) (Cat# 69479; Sigma-Aldrich) and incubating for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with 12.5 μg/ml Bis(2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophos-phate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate (Sigma Aldrich ...
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bioRxiv - Physiology 2021Quote: ... from the “AngII + L-NAME” group (n=8) were given Nω-nitro-L-arginine methyl ester (L-NAME, Sigma-Aldrich) in drinking water (40 mg/kg/d ...
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bioRxiv - Neuroscience 2021Quote: ... Fatty acids were converted to fatty acid methyl esters (FAME) by heating with 14% boron trifluoride (BF3) in methanol (Sigma) and separated on capillary columns in an HP 5890 Series II GLC equipped with a flame ionization detector ...
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bioRxiv - Neuroscience 2021Quote: ... and 5mM of lysosomal damaging compound and LRRK2 activator (24) L-leucyl-L-leucine methyl ester (LLOMe, Sigma Aldrich #L7393) dissolved in DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... Partial depletion of cholesterol was achieved by incubating the cells in 4 mM methyl-β-cyclodextrin (MβCD, Sigma Aldrich: 332615) at 37°C for 30 min or in 60 μg/ml saponin (Nacalai Tesque ...
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bioRxiv - Genetics 2020Quote: Serial ten-fold dilutions of logarithmic yeast cells were spotted on fresh Synthetic Dextrose (SD)-complete (or SD lacking a specific amino acid to preserve the plasmid) plates with or without different concentrations of Methyl methane sulfonate (MMS)(Sigma) and incubated at 30°C for three days ...
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bioRxiv - Neuroscience 2023Quote: ... the bath application of 30 μM 5-HT together with 50 μM N-methyl-DL-aspartic acid (NMA, Sigma-Aldrich) was applied to the isolated brains ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were then further incubated with 20 µL of N-(tert-butyldimethylsilyl)-N-methyl-trifluoroacetamide with 1% tert-Butyldimethylchlorosilane (TBDMS) (Sigma) at 60 °C for 60 min ...
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: Visualization of the accessible pool of cholesterol was performed after 30 min treatment with 10 mM methyl-beta-cyclodextrin (MβCB) in 1% lipoprotein-deficient serum (LPDS, Sigma #S5519) or 1% LPDS control ...
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bioRxiv - Immunology 2023Quote: ... HPLC grade methyl tert-butyl ether (MTBE), chloroform, ammonium fluoride (NH4F) (ACS reagent, ≥ 98%) and ammonium formate from Sigma-Aldrich or VWR ...
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bioRxiv - Cancer Biology 2024Quote: ... TRACER scaffolds were manufactured by infiltrating cellulose paper strips (0.5 x 12 cm, Miniminit Products) with poly(methyl methacrylate) (PMMA, 120 kDa) dissolved in acetone (Sigma-Aldrich) at 0.175 g/mL with an Allevi Beta 1 3D printer ...
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bioRxiv - Neuroscience 2024Quote: ... the bound protein was visualized using 100 µl of Tetra-methyl-benzidine liquid substrate (TMB slow kinetic form, Sigma-Aldrich) at RT for 20 min ...
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bioRxiv - Genetics 2024Quote: Serial 10-fold dilutions of logarithmic yeast cells were spotted on fresh synthetic dextrose (SD)-complete (or SD lacking a specific amino acid to preserve the plasmid) plates with or without different concentrations of methyl methanesulfonate (MMS) (Sigma) and incubated at 30°C for 3 days ...
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bioRxiv - Plant Biology 2024Quote: ... The tissue was blotted on tissue paper before weighing to remove excess water and then was transferred into a 1.5mL Eppendorf tube containing 1 mL of di-methyl sulfoxide (DMSO) (#D8418, Sigma Aldrich) and incubated in the dark at 65C with 300 rpm shaking for 45 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were treated with 1 ml pre-cooled (-20°C) mixture of methyl tert-butyl ether (MTBE, Sigma 306975), Optima LC-MS grade methanol (A456-212 ...
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bioRxiv - Biophysics 2024Quote: ... methyl viologen (MV) (catalog no. 856177-1G) and sodium hydroxide (catalog no. 30620-1KG-M) were obtained from Sigma-Aldrich. Methanol (catalog no ...
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bioRxiv - Molecular Biology 2023Quote: 2 x 106 hVSMCs were seeded in to tissue culture flasks and incubated with 15 mL of serum free complete medium (Cat. #311-500) for 24 hours under control or cholesterol treated (methyl-β-cyclodextrin-cholesterol mixture (5µg/ml, Sigma)) conditions using an established protocol35 ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were treated with 1 ml pre-cooled (-20°C) mixture of methyl tert-butyl ether (MTBE, Sigma 306975), Optima LC-MS grade methanol (A456-212 ...