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Citations for Millipore Sigma :
1501 - 1550 of 10000+ citations for 2' Methyl 3 phenylpropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... nalidixic acid (NA; N8878, BCBW6556), and 4′,6-diamidino-2-phenylindole (DAPI; D9542, 28718-90-3) were purchased from Sigma Aldrich, USA ...
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bioRxiv - Neuroscience 2020Quote: ... flies were collected 2-5 days post eclosion and grown for another 3-5 days on 1mM all-trans retinal (R2500; Sigma-Aldrich) supplemented food in complete darkness before experimental testing was performed ...
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bioRxiv - Cancer Biology 2020Quote: ... protein was extracted using RIPA buffer with added cOMPLETE™ Mini protease inhibitor cocktail (#11836153001) and phosphatase inhibitor cocktail 2 and 3 (Sigma). For 2D cell culture ...
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bioRxiv - Neuroscience 2020Quote: ... blocked and permeabilized for 1 h in 10% donkey serum/PBS-T/0.3% triton-X-100 pH 7.4 and incubated for 2 h (RT) in mouse anti-puromycin 12D10 antibody (Sigma Aldrich, MABE343), washed extensively ...
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bioRxiv - Plant Biology 2021Quote: ... The tip was broken to achieve a 2-3 µm diameter and was front-filled with potassium ionophore I (Sigma, Switzerland), a liquid ion exchanger to achieve high resistance (4–6 GΩ) ...
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bioRxiv - Plant Biology 2021Quote: SDS washed and de-starched dry onion peels were weighed and added to a 2 mL screw – top microcentrifuge tube and 1 mL of 3 M HCl in methanol (Sigma, USA) was added to the walls and incubate for 16 h at 80°C in heating block in a fume hood as per De Ruiter et al ...
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bioRxiv - Microbiology 2021Quote: ... the cultures were harvested by centrifugation (20,000 × g for 3 min at RT) and the pellet was resuspended in a volume of 2× Laemmli buffer (Sigma-Aldrich) that adjusts for the total number of bacteria according to the OD600 of the initial cultures followed by 15 min incubation at 95 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Seeds were surface sterilized and kept for stratification in dark at 4 °C for 2-3 days on half strength MS-agar (Millipore Sigma) plates ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (16) ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was concentrated to 2-3 mg ml-1 using an Amicon ultracel-100 centrifugal filter unit (EMD Millipore) for amphipol exchange or nanodisc reconstitution ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were then washed 3X with EGM-2 medium and re-suspended in PBS containing 3 mg/ml of fibrinogen (Sigma, #F8630) and 0.15 U/ml of aprotinin (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
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bioRxiv - Immunology 2022Quote: ... and mesenteric) (n=3) were pooled in digestion medium consisting of RPMI 1640 with 2% fetal bovine serum (FBS) (Sigma-Aldrich), deoxyribonuclease (DNase ...
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bioRxiv - Neuroscience 2022Quote: ... was placed above the platform to support 2 to 3 drops of high concentrated glucose on the CS+ numerical set stimuli (1.5M, Sigma Aldrich G8270) and 2 to 3 drops of EVIAN water on the CS-numerical set condition ...
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bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
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bioRxiv - Cell Biology 2022Quote: The neuroprotective effect of CA against 6-OHDA was evaluated by MTT reagent (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) (Sigma Aldrich, UK, M5655) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2020Quote: Fluorescent immunostaining for nuclear NFκB and cytoplasmic NFκB was quantified in immortalized C8D30 astrocytes following 2-hr treatment with 1μg/ml LPS or a cocktail of 3 cytokines: Il-1α (3ng/ml, Sigma, Cat# I3901), TNFα (30ng/ml ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then treated for 15 min up to 4 h at 37°C with 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, #M5655-1G) in 5 mM PBS ...
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bioRxiv - Cell Biology 2020Quote: Fully differentiated C2C12 MS2-CAS myotubes were starved in serum-free DMEM for 2 hrs before a 3-hr incubation in glucose-free or low-glucose DMEM/fatty acid-free BSA (Sigma-Aldrich) 0.2% supplemented with a mixture of cold and radiolabeled palmitate (Palmitic Acid ...
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bioRxiv - Microbiology 2021Quote: ... Samples were stained for DAPI diluted in water for 2 min and washed 3 times with water before drying cells on poly-L-lysine (Sigma, P8920) coated coverslips at room temperature protected from light ...
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bioRxiv - Cancer Biology 2022Quote: ... Mice were induced to form tumors at 2-3 months of age by administration of tamoxifen (Sigma-Aldrich, St. Louis, MO) using 100 mg/kg (in corn oil ...
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bioRxiv - Cell Biology 2022Quote: ... Cells from each donor animal were plated as separate cultures in serum-free growth medium42 (SFGM, Supplementary Table 3) on fibronectin-coated (4 μg cm−2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels for 72 h prior to FACS ...
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bioRxiv - Neuroscience 2020Quote: The viability of microglial cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich, Steinheim, Germany) assay as described previously [36] ...
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bioRxiv - Systems Biology 2020Quote: ... 120μL of 1:1 DTNB:GR solution (DTNB: 2mg 5,5’-dithiobis(2-nitrobenzoic acid) (Sigma-Aldrich; Cat#D8130; CAS: 69-78-3) in 3mL KPE ...
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bioRxiv - Microbiology 2020Quote: ... were passaged 2-3 times weekly and grown at 37° C with 5% CO2 in DMEM containing 10 mM L-glutamine (Sigma, M8537), supplemented with 10% heat-inactivated FBS (HyClone) ...
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bioRxiv - Microbiology 2021Quote: ... or the same isosmotic solution with 100 µM of the general anion channel inhibitor 5-Nitro-2-(3-phenylpropylamino) benzoic acid (NPPB, Sigma-Aldrich), which inhibits malarial new permeation pathways (59) ...
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bioRxiv - Cancer Biology 2021Quote: ... targeting EZH2 (shEZH2#3 [TRCN0000040076] and shEZH2#4 [TRCN0000010475]) and FAK (shFAK#2 [TRCN0000121211], shFAK#3 [TRCN0000121318], and shFAK#21 [TRCN0000121321]) were purchased from Sigma-Aldrich. pRK5-TGFβRI-FLAG plasmid (#14833 ...
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bioRxiv - Developmental Biology 2021Quote: ... stage embryos were isolated from oviducts in M2 media (pre-warmed at 37°C for at least 2-3 hours) and thereafter cultured in KSOM (Sigma-Aldrich, EmbryoMax KSOM Mouse Embryo Media ...
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bioRxiv - Neuroscience 2021Quote: ... a mouse monoclonal anti-chondroitin sulfate antibody (clone CS-56, 1:500 for Experiment 1 and 3 or 1:1000 for Experiment 2, C8035, Sigma Aldrich) specific for the glycosaminoglycan portion of the chondroitin sulfate proteoglycans that are the main components of the PNN and a polyclonal rabbit anti-parvalbumin antibody (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Allicin was synthesized by oxidation of 3-[(Prop-2-en-1-yl)disulfanyl]prop-1-ene (diallyl disulfide, Sigma-Aldrich, Germany) with peracetic-acid (glacial acetic acid/H2O2 ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were then washed 3X with EGM-2 medium and re-suspended in PBS containing 3 mg/ml of fibrinogen (Sigma, #F8630) and 0.15 U/ml of aprotinin (Sigma ...
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bioRxiv - Microbiology 2022Quote: Seventy-two hour post-fertilised larvae were first anaesthetized for 2 - 3 min in embryo medium E3 containing 0.2 mg/mL Tricaine (Sigma-Aldrich, USA). After which ...
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bioRxiv - Microbiology 2023Quote: Cross-linking experiments were performed by incubation of 2 mM and 5 mM of suberic-bis-acid ester (3-sulfo-N-hydroxyuccinimy (BS3, Sigma-Aldrich) with the purified recombinant protein in 25 mM sodium phosphate pH 7.4 for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... culturing in liquid media used 10 mL of a mixture of a 2:3 ratio of Todd-Hewitt broth (Sigma-Aldrich) with 0.5% yeast extract (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... Glass coverslips were plasma activated and incubated with a solution of 2% (v/v) 3-(trimethoxysilyl)propyl methacrylate (440159-500ML, Sigma Aldrich) and 1% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... samples were treated for 2 h at room temperature with 3% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA), and 0.3% PBS-T as a blocking agent ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained with freshly made Oil Red O solution (2 parts Milli-Q and 3 parts Oil Red O, Sigma Aldrich). Osteocytes were fixed with ice-cold 70% ethanol at 4°C and stained with freshly prepared supernatant of saturated Alizarin Red S (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were next incubated in 3 mL DME/F-12 medium with 2 units/mL DNase I (Sigma-Aldrich, D4263) at room temperature for 3 min and centrifuged at 450 g for 10 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each well was then topped with 120 µL of PB(2×)M9 (various glucose concentration) and 10 µL of fluorescein (FITC)dextran (3 kDa, anionic, Sigma-Aldrich) solution in PBS to a total volume of 240 µL ...
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bioRxiv - Cell Biology 2023Quote: ... twice with wash buffer 3 (20 mM Tris-HCl pH 7.5, 2 M NaCl and 0.5% IGEPAL CA-630 [Sigma-Aldrich, cat#I8896]), and twice with wash buffer 4 (50 mM Tris-HCl pH 7.5) ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysates were sonicated on ice with a microtip sonicator (120 W, 8 s on, 2 s off, 3 pulses) and supplemented with 200 U of benzonase (Sigma; E8263). The lysates were cleared by centrifugation (18000 g ...
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bioRxiv - Immunology 2022Quote: ... After washed with PBS for 3 times and stained with DAPI (4’,6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... expressing HUVECs (GFP, Olaf Pharmaceuticals, Worcester, MA, USA) were expanded in EGM-2 with 3% extra FBS (FBS, BCBV7611, Sigma-Aldrich) to passage 8 ...
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bioRxiv - Neuroscience 2024Quote: ... Cre-mediated recombination was induced in mice 3-7 months of age by intraperitoneal injection of 2 mg/day tamoxifen (Sigma-Aldrich) diluted with 300 μl corn oil (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were harvested and enzymatically dissociated into a single-cell suspension in PBS supplemented with 3% fetal bovine serum (#FB-1101, Biosera) and 2 mM EDTA (#ED2SS, Sigma=:Aldrich). After 15 minutes of incubation with 5 nM SYTOX™ Red Dead Cell Stain (#S34859 ...
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bioRxiv - Cell Biology 2024Quote: We assessed mitochondrial activity using the 3-(4,5-dimethylthiazol-2-yl-)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (Sigma-Aldrich, Cat# M6494). As a tetrazolium dye ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% TritonX100 in 2 × SSC), anti-bleaching buffer (50 mM Tris-HCl pH 8, 300 mM NaCl, 3 mM Trolox (Sigma 238813), 0.8% D-glucose (Nacalai Tesque 168060-25) ...
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bioRxiv - Plant Biology 2023Quote: ... Flash-frozen plant samples (0.2 g) were ground and extracted using 2 mL of 3 % 5-sulfosalicylic acid (Sigma, ON, Canada). The extract was centrifuged for 10 min at 8050 x g at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by the addition of 1 mg/mL MTT reagent (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma Aldrich, Darmstadt, Germany). After the incubation period of 4 h ...
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bioRxiv - Molecular Biology 2023Quote: ... An anti-bleaching buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 2×SSC, 3 mM trolox (Sigma-Aldrich, 238813), 0.8% D-(+)-Glucose (Nacalai Tesque ...