Labshake search
Citations for Millipore Sigma :
1451 - 1500 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... plates were treated with 3-amino-propyltrimethoxysilane (diluted 1:2 with PBS; Sigma-Aldrich/Merck) for 15min at RT ...
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Biophysics 2021Quote: ... was performed using a pre-annealed DNA-RNA template (DNA45: 5’-TTCTTT ATCTCTTATTTCCTTCTATTTTCCACGCGCCTTCTATTT-3’; RNA13: 5’-[6FAM]-GGCGCGUGGAAAA-3’, Sigma Aldrich). The reaction buffer comprised 25 mM HEPES pH 7.2 ...
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bioRxiv - Neuroscience 2019Quote: ... KO2 along with Cas9 donor plasmid were used as template DNA and amplified with F 5’-GAAGAAGAATGGCCTGTTCG-3’ and R 5’-GCCTTATCCAGTTCGCTCAG-3’ with KOD Xtreme (Novagen, #71975). Cycling conditions were as follows ...
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bioRxiv - Cancer Biology 2022Quote: ... 53 from the RNAi Consortium (clones TRCN0000087290 and TRCN0000087292 with target sequence 5’-GCGGGAGTATAGTGAGTTTAA-3’ and 5’-CGGACAGTTTGGCACAATCAA-3’, respectively, distributed by Sigma-Aldrich, Merck ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial DNA was used to amplify the entire 16S region by PCR [5′-AGAGTTTGATCCTGGCTCAG-3′ (forward) and 5′-AAGGAGGTGATCCAGCCGCA-3′ (reverse)] using Expand High-Fidelity Polymerase (Sigma-Aldrich). The PCR products were purified using the QIAquick PCR Purification kit (Qiagen) ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EDTA with freshly added 1 mM DTT), then concentrated to 500 μl using Amicon Ultra-4 (4 mL, 3 kDa cut-off) (Millipore, Darmstadt, Germany, UFC800324) and loaded onto a Superdex75 10/300 GL size exclusion column (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... to which a 5 % of fluoro-surfactant was added (1H,1H,2H,2H-Perfluoro-1-octanol, CAS Nr 647-42-7, Sigma-Aldrich).
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bioRxiv - Biochemistry 2023Quote: Female mice (n=5-7 per group) were fasted overnight and then injected with poloxamer 407 intraperitoneally (1 g/kg BW) (Sigma, 16758). Plasma TG concentrations were measured at 0 ...
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bioRxiv - Biochemistry 2024Quote: Reaction mixes were prepared with the desired ⍺β-tubulin concentrations (7 μM or 10 μM) containing 5% Alexa 568-tubulin plus 1 mg/ml BSA (Sigma) in assay buffer (BRB80 with 30 mM KCl ...
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bioRxiv - Immunology 2021Quote: ... 2 μL Betaine (5 M Sigma), 0.5 μL DTT (100 mM) ...
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bioRxiv - Microbiology 2019Quote: ... with 5% 2-mercaptoethanol (Sigma-Aldrich). Samples were boiled for 5 minutes at 95°C before separation on a gradient 4-20% SDS-PAGE gel (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... with 5% 2-mercaptoethanol (Sigma M6250), and boiled for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: 5-aza-2’-deoxycytidine (Sigma A3656) was dissolved to 10 mM in sterile water and frozen in one-time-use aliquots at −80°C ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-Bromo-2’-deoxyuridine (Sigma, B5002), RNase A 19101 (17,500 U ...
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bioRxiv - Microbiology 2021Quote: ... DiSC3(5) (2 µM; Sigma Aldrich), Sytox Green (50 ng/ml ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μL Betaine (5 M Sigma), 0.5 μL DTT (100 mM ...
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bioRxiv - Neuroscience 2019Quote: ... containing 5% 2-mercaptoethanol (Sigma M6250), and fractionated by SDS-PAGE using the Mini-PROTEAN Tetra System and pre-cast TGX™ Gels (Bio-Rad #456-1096) ...
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bioRxiv - Developmental Biology 2020Quote: 5-Bromo-2′-deoxyuridine (BrdU, Sigma) was dissolved in drinking water at a final concentration of 0.8 mg/ml and given to P28.5 mice for 7 consecutive days ...
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bioRxiv - Neuroscience 2021Quote: ... 1µM 5-fluoro-2’-deoxyuridine (Sigma), penicillin and streptomycin ...
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bioRxiv - Immunology 2022Quote: ... and 2-5 mM ATP (Sigma). When indicated ...
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bioRxiv - Microbiology 2022Quote: ... with 5% 2- mercaptoethanol (Sigma-Aldrich). Samples were boiled for 5 min at 95°C before separation on a gradient 4 to 20% sodium dodecyl sulfate (SDS)-polyacrylamide gel (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... 5-fluoro-2’-deoxyuridine (Sigma-Aldrich) was added at a final concentration of 7.15 μg/ml to inhibit glial growth (Vossel et al ...
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bioRxiv - Immunology 2022Quote: ... and 2-5 mM ATP (Sigma).
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bioRxiv - Neuroscience 2024Quote: ... containing 5% 2-mercaptoethanol (Sigma-Aldrich) and loaded directly onto 4-20% Tris-Glycine mini gels (Thermo Fisher XP04205BOX ...
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bioRxiv - Microbiology 2024Quote: ... with 5% 2-mercaptoethanol (Sigma Aldrich). Samples were boiled for 10 minutes at 95°C before separation on a gradient of 4-20% SDS-PAGE gels (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... 5-Bromo-2’-Deoxyuridine (Sigma Aldrich), talazoparib (Selleckchem) ...
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bioRxiv - Cancer Biology 2023Quote: ... 5-Aza-2’-deoxycytidine (Sigma, A3656), GSK126 (MedChemExpress ...
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bioRxiv - Neuroscience 2023Quote: ... 5-bromo-2’-deoxyuridine (BrdU, Sigma) was prepared fresh daily by dissolving BrdU at 20 mg/mL in 1X PBS at 37°C and then sterile filtered via 0.22µm syringe filter ...
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bioRxiv - Neuroscience 2024Quote: ... 1mM 5-fluoro-2’-deoxyuridine (Sigma), 1mM uridine (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... 5-Fluoro-2’-deoxyuridine (Sigma-Aldrich) was typically added at a final concentration of 16 uM at DIV 7–9 to limit non-neuronal cell growth ...
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bioRxiv - Bioengineering 2020Quote: ... nalidixic acid (NA; N8878, BCBW6556), and 4′,6-diamidino-2-phenylindole (DAPI; D9542, 28718-90-3) were purchased from Sigma Aldrich, USA ...
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bioRxiv - Cell Biology 2022Quote: ... Cells from each donor animal were plated as separate cultures in serum-free growth medium42 (SFGM, Supplementary Table 3) on fibronectin-coated (4 μg cm−2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels for 72 h prior to FACS ...
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bioRxiv - Cancer Biology 2021Quote: ... targeting EZH2 (shEZH2#3 [TRCN0000040076] and shEZH2#4 [TRCN0000010475]) and FAK (shFAK#2 [TRCN0000121211], shFAK#3 [TRCN0000121318], and shFAK#21 [TRCN0000121321]) were purchased from Sigma-Aldrich. pRK5-TGFβRI-FLAG plasmid (#14833 ...
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bioRxiv - Immunology 2022Quote: ... After washed with PBS for 3 times and stained with DAPI (4’,6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... for one hour and followed with incubation overnight with following primary antibodies in 5% bovine serum albumin (Puromycin 1:2000 (Millipore), GAPDH 1:1000 (Cell Signaling Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... at 60°C for one hour and then alkylated with 50 mM iodoacetamide (Sigma) at 45°C for a few minutes in the dark ...
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bioRxiv - Immunology 2023Quote: ... and 2 x 10-5 M 2-mercaptoethanol (Sigma-Aldrich) and used directly in functional experiments.
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bioRxiv - Biochemistry 2022Quote: ... 3-[2-(2-aminoethylamino)-ethylamino] propyl-trimethoxysilane (DETA) (Sigma-Aldrich, Hamburg, Germany) for microtubule immobilization ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were blocked with goat serum diluted 1:200 in PBS for one hour followed by an overnight incubation at 4°C with the primary antibody (HIF-1α, 1:200, described in32; BACH-1, 1:200, Sigma Aldrich HPA034949) Next ...
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bioRxiv - Immunology 2022Quote: ... or the ACE2 product Ang (1-7) (Sigma, #A9202; 1 μM) in the same culture conditions described above.
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bioRxiv - Biochemistry 2022Quote: ... and 0.5 μM (with apoptosome at a molar ratio of 1:5:10 caspase-9 : Apaf-1 : cytochrome C (SIGMA)) in the following buffer conditions ...
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bioRxiv - Cell Biology 2019Quote: ... After washing 3–4 X with 1× PBS-T the strips were incubated with 1:5000 mouse anti-GST antibodies (Sigma). The secondary antibodies (anti-rabbit-HRP or anti-mouse-HRP ...
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bioRxiv - Immunology 2023Quote: ... Th17 cells were split (usually 1:3) and transduced by adding 1:80-diluted retroviral supernatant and 4 µg/ml polybrene (Sigma) in addition to 10 ng/ml IL-23 and 10 µg/ml anti-IFNγ antibody (all diluted in pre-warmed T cell medium and calculated for culture volume) ...
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bioRxiv - Pathology 2022Quote: ... samples in TRIzol were separated using bromo-chloro-propane (Sigma, St. Louis, MO). The aqueous phase was collected into a new tube and glycogen was added as a carrier ...
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bioRxiv - Cancer Biology 2021Quote: Sub-confluent cells were labelled with 25 µM chloro-deoxyuridine (CldU) (Sigma Aldrich) before media was removed and replaced with fresh media containing 250 µM iodo-deoxyuridine (IdU ...
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bioRxiv - Cancer Biology 2023Quote: ... The reaction was stopped using 10% Tri Chloro Acetic Acid (Sigma; Cat# T6399) and kept at 4°C for 30 min ...
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bioRxiv - Immunology 2024Quote: ... chloro-acetamidomethylated polysulfone was prepared by amidomethylation of polysulfone (Mn ∼22,000; Sigma-Aldrich) with N-hydroxymethyl-2-chloroacetamide ...
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bioRxiv - Evolutionary Biology 2022Quote: ... consisting of 4% (v/v) of 19:1 acrylamide/bis-acrylamide (Sigma-Aldrich, A9926-5), 60 mM Tris·HCl pH 8 ...
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bioRxiv - Neuroscience 2021Quote: ... nonspecific staining was blocked with blocking solution (TBST and 4% normal donkey or normal goat serum) and incubated overnight at 4°C with SOX9 antibody (1:2000, Millipore, AB5535, RRID: AB_2239761). Sections were washed and incubated with Alexa 568-conjugated secondary antibody (1:5000 ...