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Citations for Millipore Sigma :
1401 - 1450 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... and then stained for 2 minutes at room temperature using filtered 0.5% Solvent Black 3 (CAS Number 4197-25-5; Sigma 199664) dissolved in 75% ethanol ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 µl of a solution of 5 mg/mL MTT [3-(4,5)-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma Chemical Co.) was added ...
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bioRxiv - Immunology 2019Quote: ... Fluorescein isothiocyanate (FITC) [2-(6-hydroxy-3-oxo-3h-xanthen-9-yl)-5-isothiocyanatobenzoic acid] was purchased from Sigma-Aldrich Korea ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were exposed to 5 μM CHIR99021 (Axon) between days 2 and 3 and then supplemented with 100 nM RA (Sigma) until day 5 ...
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bioRxiv - Cell Biology 2019Quote: ... 5’-GGTCAGCCAACTCGTCACAGTC-3’) and by alizarin red S staining with a 2% (w/v) alizarin red S solution (Sigma-Aldrich). Slides were imaged in an Olympus BX61 microscope ...
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bioRxiv - Cell Biology 2021Quote: Confluent ESCs monolayers were decidualized in DMEM/F12 containing 2 % FBS supplemented with 0.3 mM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (cAMP) (Sigma-Aldrich, USA), 10 nM β-Estradiol (E2 ...
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bioRxiv - Genetics 2024Quote: ... differentiation of iPSCs was induced through the Wnt modulation method with 7 μM glycogen synthase kinase 3 b (GSK3b) inhibitor CHIR99021 (Selleckchem) and 5 μM inhibitor of WNT production 2 (IWP2) (Sigma). Basal Media was composed of RPMI media supplemented with B27 (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... 3 µL of 2 M CaCl2 and 5 µL of 10 mg/mL ε-aminocaproic acid (ε-ACA) (Sigma-Aldrich), and 1 µL of 1U/µL Thrombin (MP Biomedicals ...
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bioRxiv - Neuroscience 2023Quote: ... Then the brains were dissected to 2 mm slices and incubated for 10 minutes in freshly prepared 0.5% TTC solution (2, 3, 5-Triphenyltetrazolium chloride, Sigma-Aldrich). Afterward ...
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bioRxiv - Cancer Biology 2023Quote: ... two to four-month-old mice were gavaged 3 times over 5 days with 2 mg tamoxifen (TX; Sigma #T5648) in corn oil (Sigma #C8267) ...
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bioRxiv - Cell Biology 2020Quote: ... and 7 μL of 100 mg/mL glyceraldehyde-3-phosphate (Cayman Scientific or Sigma Aldrich) from frozen stocks ...
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bioRxiv - Molecular Biology 2019Quote: ... 3) Deferoxamine mesylate salt (DFOA, Sigma Aldrich, Saint Louis, MI, USA, CAS: 138-14-7) at 1μM concentration 4 ...
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bioRxiv - Bioengineering 2019Quote: ... and the pellets were resuspended with room temperature HPLC-grade 3:7 methanol:acetonitrile (Sigma Aldrich) and centrifuged at 13,000 rpm for 5 minutes ...
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bioRxiv - Immunology 2023Quote: The following small compound inhibitors and reagents were used: caspase-3/7 inhibitor I (Sigma), CRID3 (MCC-950 ...
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bioRxiv - Cell Biology 2020Quote: ... then 85°C for 10 minutes and transfected in a 3:1 polyethylenimine (PEI, Sigma Aldrich, 408747):DNA ratio into 70% confluent HEK 293A cells (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... DNA probes (5’-GTTATGAGCCCGACGAGCTACCAGGCTGCT-3’) with a 5’-ethylcarbamate amino linker (Sigma-Aldrich) were covalently immobilized on NHS-activated Sepharose 4 Fast Flow (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... Seedlings at the one leaf stage were treated in 1/2 Hogland solution (pH 6.0) containing 1.5% NaCl (Sigma-Aldrich) or 20% polyethylene glycol (PEG ...
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bioRxiv - Neuroscience 2023Quote: ... mice were presented with i) two bottle of water ii) one bottle of water and one bottle of sucrose (5%, Sigma Aldrich) iii ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... or α/β-tubulin (1:1 mixture) (1:100 in PBS; 2 hr at 37°C) (Sigma; T9026, T4026, USA). FITC-conjugated anti-mouse IgG (Jackson ImmunoResearch ...
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bioRxiv - Bioengineering 2020Quote: ... 7-hydroxycoumarin (7-HC, Sigma-Aldrich), generated from coumarin was quantified via fluorescence detection (excitation/emission ...
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bioRxiv - Neuroscience 2024Quote: Acute PHP was induced by incubating semi-intact preparations in 20 µM Philanthotoxin-433 (Fig. 4: PhTx; Santa Cruz, sc-255421, Lot B1417 and Fig. 7: PhTx; Sigma Aldrich, P207-2, Lot MKCK7405) diluted in HL3 containing 0.4 mM Ca2+ for 10 minutes at room temperature (Frank et al ...
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bioRxiv - Immunology 2020Quote: ... two STAT-3 inhibitors (STAT-3 inhibitor III (WP-1066) and STAT-3 inhibitor XIII (C-188-9) were tested (Merck, Sigma) and WP-1066 was used for infection studies at a final concentration of 12 μM ...
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bioRxiv - Developmental Biology 2022Quote: Samples were incubated with primary antibody overnight at 4°C in PBT (PBS with 3% Triton X-100 (Sigma-Aldrich X100-100 mL)) with 5% NGS (Capralogics GS0250) ...
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bioRxiv - Plant Biology 2019Quote: ... surface sterilized and stored at 4 °C for 3 days before growing on MS agar (1.1% w/v) containing 100 µg/mL kanamycin (Sigma-Aldrich, St. Louis, MO). The proportion of WT to dgk4-1 seeds were scored after 7 days of growth.
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bioRxiv - Developmental Biology 2021Quote: ... Sections were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) (1:3000; Sigma-Aldrich) and coverslips were mounted with FluoroGel (Electron Microscopy Services ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1:10000; Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2021Quote: DOI or 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane was obtained from Sigma-Aldrich. Vehicle refers to saline (0.9%) ...
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bioRxiv - Physiology 2019Quote: ... Nuclei were stained with DAPI (1:1000; 4’,6-diamidino-2-phenylindole dihydrochloride; Sigma).
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bioRxiv - Cell Biology 2020Quote: ... 4′,6-diamidino-2-phenylindole (DAPI, Sigma #D9542, 1:1000 for 10min at RT) Mice were deeply anesthetized with isoflurane and then transcardially perfused with ice-cold heparinized PBS (25U/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... slices were incubated with 4′,6-diamidino-2-phenylindole (DAPI; 1:500, Sigma-Aldrich) to counterstain cell nuclei ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) (1:3000; Sigma-Aldrich) and coverslips were mounted with FluoroGel (Electron Microscopy Services ...
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bioRxiv - Neuroscience 2022Quote: ... After staining nuclei with DAPI (1:1000, 4’, 6-diamidino-2-phenylindole dihydrochloride; Sigma) and several washes with PBST ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) (1:3000; Sigma-Aldrich), and coverslips were mounted with FluoroGel (Electron Microscopy Services ...
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bioRxiv - Bioengineering 2021Quote: ... and (3) 4- methylmorpholine (4.5 equiv:ELP amine; Sigma, M56557). The reagents are dissolved at room temperature (RT ...
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bioRxiv - Developmental Biology 2022Quote: ... CHIR99021 [3 µM] and 4.5x10-4 M Monothioglycerol (Sigma). On day 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 and 3 kDa (Amicon Ultra-4, Merck Millipore) to fractionate the proteins according to their size ...
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bioRxiv - Bioengineering 2021Quote: ... stored for 30 min at 4°C and fixed with 4% paraformaldehyde (Sigma Aldrich) for 10 min at RT ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were fixed overnight (∼16 hours) at 4°C in 4% paraformaldehyde (Sigma 158127) + 20 mM EPPS (Sigma E1894) ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 200 ml of chilled (4°C) 4% fresh paraformaldehyde (PFA, Sigma, P6148) at 20 ml/min ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 200 ml of chilled (4°C) 4% fresh paraformaldehyde (PFA, Sigma, P6148) at 20 ml/min ...
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bioRxiv - Microbiology 2021Quote: Adult female worms were incubated with 5 μM of 5’ cy3-labeled Bma-lad-2 siRNA 1 (Sigma Aldrich) for 24 hrs to evaluate uptake of siRNA into intestinal tract epithelial cells ...
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bioRxiv - Neuroscience 2020Quote: ... Bacterial pellets of 20 OD600 units were lysed in 0.5 ml cold lysis buffer (50 mM Sodium-phosphate pH 8.0, 300 mM NaCl, 5% glycerol, 5 mM 2-Mercaptoethanol, 1 mM PMSF [Sigma]), RNase [0.01 mg/ml] ...
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bioRxiv - Biochemistry 2021Quote: ... and PsnB variants (single concentrations at 0.4-2 μM) were co-incubated at 37°C in a buffer containing ATP (5 mM; Sigma-Aldrich), NADH (400 μM ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 30 minutes at 37°C prior to lysis with 2% SDS in PBS containing 5 U/μl benzonase (Sigma). Cell lysates were heated for 10 minutes at 95°C and centrifuged at 21000 x g for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... cells were grown on coverslips and incubated at 37°C for 2 hrs with either actinomycin D (5 μg/ml, Sigma) or DRB (50 μg/ml ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were maintained at 37°C in 5% CO2 atmosphere and treated with 2 µg/ml tunicamycin (Sigma-Aldrich), 10 nM-1µM thapsigargin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were incubated for 1 – 1.5 h at 37°C followed by addition of warm DMEM with 5% fetal bovine serum and 2% carboxymethyl cellulose (CMC; Sigma) to achieve a 1% CMC overlay ...
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bioRxiv - Neuroscience 2022Quote: ... and 1% each of Phosphatase Inhibitor Cocktails 2 and 3 (Millipore Sigma #P5726 and #P0044). Cells were scraped ...
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bioRxiv - Cell Biology 2023Quote: ... was used at 1 µM and ARP 2/3 complex inhibitor - CK666 (182515; Sigma-Aldrich) was used at 100 µM concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1% each of phosphatase inhibitor cocktails 2 and 3 (Sigma, Saint Louis, MO, USA). Particulate was removed by centrifugation of lysates at 13,000 rpm for 10 minutes at 4°C and filtration through 0.45 µm syringe filters and lysates were stored at -80°C until preparation for MIB-MS ...