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Citations for Millipore Sigma :
1351 - 1400 of 10000+ citations for Recombinant Mouse Icam2 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... were used in this work: The perfluorinated amine Fluorinert™ FC-40 was purchased from Sigma-Aldrich (Overijse, Belgium), while the perfluorinated ether FC-770 and the perfluorinated amine FC-3283 were obtained from Acros Organics (Geel ...
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bioRxiv - Immunology 2020Quote: ... Intestines were then incubated with 20 mL RPMI + 10% FCS +1% PS + 2.5 U/mL Collagenase VIII (Sigma-Aldrich) + 2 U/mL DNAse I (Roche) ...
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bioRxiv - Cancer Biology 2019Quote: ... cell pellets were resuspended with 10% FCS and fixed by adding an equal volume of 4% formaldehyde (Sigma-Aldrich) and mixed for 10 min at room temperature while shaking ...
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bioRxiv - Neuroscience 2020Quote: ... Plates were washed with PBST and incubated with a goat anti-human Fc−peroxidase antibody diluted 1:5,000 (Sigma) in blocking buffer for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... The plates were then washed and incubated with a 1:5,000 dilution of peroxidase-conjugated anti-human Fc antibody (Sigma) for 1 hr ...
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bioRxiv - Microbiology 2019Quote: ... washed cells were resuspended in incubation buffer (IB; Hank’s balanced salt solution, 2% dialysed FCS, 1x RPMI Amino Acids Solution (Sigma), 2 mM L-glutamine ...
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bioRxiv - Microbiology 2021Quote: ... Purified ACE2-Fc was concentrated and buffer exchanged into PBS by a 50-kDa MWCO ultra centrifugal filter (Millipore).
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bioRxiv - Immunology 2019Quote: ... Intestines were then incubated with 20 mL RPMI + 10% FCS +1% PS + 2.5 U/mL Collagenase VIII (Sigma-Aldrich) + 2 U/mL DNAse I (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... were cultured in Eagle’s Minimum Essential Medium (ATCC, Middlesex, UK) supplemented with 10% FCS and 0.8 mg/ml geneticin (Sigma-Aldrich). All cells were cultured at 37°C and 5% CO2 in humidified incubators and were free of mycoplasma contamination.
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bioRxiv - Immunology 2021Quote: ... cells were incubated in medium containing 2% FCS with or without the PAR2 negative allosteric modulator (AZ3451, Sigma Aldrich) or a NF-κβ inhibitor (Bay11-0782 ...
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bioRxiv - Microbiology 2020Quote: ... FMC Biopolymer (90) in DMEM (containing 2% FCS and antibiotics) and serial dilutions of 5-FU (F6627, Sigma-Aldrich) or Ribavirin (R9644 ...
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bioRxiv - Microbiology 2020Quote: ... The mice were boosted subcutaneously (s.c.) at day 8 with 50 μg of RBD-Fc plus Freund’s Adjuvant Complete (Sigma, USA) and at day 13 with 50 μg of RBD-Fc plus Titermax adjuvant (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... Digestion of the colon tissue was performed in RPMI/10% FCS containing 0.4mg/ml type VIII collagenase (Sigma Aldrich) and 40μg/ml DNase I (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Inocula were removed and replaced with DMEM containing 1% FCS and a semi-solid overlay consisting of 1.5% carboxymethyl cellulose (SIGMA). Cells were incubated for 72h ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed in RPMI supplemented with 10% FCS and incubated with 10 μM of MG132 (Sigma Aldrich) for 3h ...
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bioRxiv - Microbiology 2023Quote: ... Parasites containing restored S14 loci were selected by negative selection using a 5-fluorocytosine (5-FC) drug (Sigma, USA) as previously described (Srivastava & Mishra ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were frozen at 1 x 107 cells per 1 ml of freezing medium (FCS, 10 % DMSO (Sigma-Aldrich)) ...
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bioRxiv - Genetics 2023Quote: ... in supplemented RPMI1640 medium (10% heat-inactivated FCS, 2mM L-glutamine, 50IU/ml penicillin, 50μg/ml streptomycin; Sigma-Aldrich), 1×106 MLN cells/well were stimulated by 50ng/ml PMA and 1μM ionomycin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Eurobio Scientific, CVFSVF00-01) and containing 1% penicillin-streptomycin (Sigma-Aldrich, P4333). HEK-293T were grown in Hepes-modified DMEM (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... and digested in 20 mL of DMEM added with 20% FCS and 100 U/ml of collagenase (Sigma-Aldrich) for 40 min at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... at a density of ∼2.5*106 cells/mL in 5% fetal calf serum (FCS) (Bodinco BDC, 16941) DMEM (Dulbecco’s Modified Eagle’s Serum (Sigma, 05796), 1% Pen-Strep (Lonza ...
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bioRxiv - Genomics 2024Quote: ... at a density of ∼2.5*106 cells/mL in 5% fetal calf serum (FCS) (Bodinco BDC, 16941) DMEM (Dulbecco’s Modified Eagle’s Serum (Sigma, 05796), 1% Pen-Strep (Lonza ...
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bioRxiv - Cancer Biology 2024Quote: ... taken up in 40ml of FCS-free DMEM supplemented with 480 μl of 10 mg/ml Trypsin (Sigma, T9935) and 800 μl of 50 mg/ml Collagenase II (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... ELISA plates were blocked for 1 h in AD before addition of assay samples for 1 h followed by incubation with 1/20,000 dilution Goat anti-human Fc-HRP (Sigma). All steps were conducted at 37°C and separated by three washes with PBS-T (0.05% Tween-20) ...
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bioRxiv - Microbiology 2024Quote: ... precoated with fibronectin-collagen IV (10 μg.ml-1) and grown in DMEM containing 10% Fetal Calf Serum (FCS) and 1 μM dexamethasone (Sigma) for 14 days to allow proper differentiation ...
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bioRxiv - Neuroscience 2024Quote: ... Forty-eight hours after transfection the medium with soluble RGMA-Fc or APP was concentrated through a 50,000 kDa column (YM-50, Millipore). The concentrated proteins were supplemented with Dulbecco’s modified Eagle’s medium 1 g/L glucose (DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated with anti-mouse NeuN or anti-mouse parvalbumin (Millipore, MAB377 ...
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bioRxiv - Neuroscience 2020Quote: ... and 3) mouse anti-NeuN (mouse monoclonal, Millipore, cat # MAB377, 1:100); and mouse anti-S100β (Sigma S2532 ...
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bioRxiv - Microbiology 2021Quote: ... a mouse anti-FLAG primary (Sigma Mouse Anti-FLAG M2 monoclonal antibody) was used ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-mouse β-tubulin III (1:400, Millipore Sigma, catalog T2200), mouse humanized anti-GM2 (1:400 ...
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bioRxiv - Immunology 2022Quote: ... ng mouse IgG was generated using purified mouse IgG (Sigma Cat #15381); absorbance values from this standard curve were used to convert sample absorbance signals into mass equivalents for both anti-S and anti-N antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-pERK1/2 (T185/Y187) and mouse anti-Flag M2 (Sigma); and mouse anti-H3 and rabbit anti-H3K4 trimethyl (Active Motif).
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bioRxiv - Cancer Biology 2022Quote: ... For metastases delimitation cytokeratin (mouse anti-mouse cytokeratin pan-mixture, ascites, Sigma) and luciferase (rabbit anti-firefly luciferase ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Biochemistry 2019Quote: 25 µl of 100 µM recombinant purified GST Pat1-NC was bound to 30 µl GST-slurry (Sigma), and one aliquot of GST-beads was included without added Pat1-NC as negative control ...
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bioRxiv - Immunology 2019Quote: ... supplemented with cytokine cocktails aimed at proliferation (50 ng/ml murine recombinant thrombopoietin (TPO, Sigma-Aldrich SRP3236-10UG) and 100ng/ml Stem Cell Factor (SCF) ...
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bioRxiv - Immunology 2019Quote: ... NMRI mice were injected subcutaneously at two different locations with 100µg of the recombinant Thio-EmACT resuspended in 100µl Freund Incomplete adjuvant (Sigma). The double injections were repeated four weeks later to boost the mice anti-EmACT response ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant protein was then isolated from the supernatant by nickel affinity chromatography using loose Ni-NTA resin (Sigma). Protein eluted from Ni-NTA resin was filtered and then loaded onto a size exclusion column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2021Quote: ... FAK) recombinant proteins to be labeled with Alexa fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Bioengineering 2022Quote: ... Epidermal growth factor (EGF) and Recombinant Human Fibroblast Growth Factor Basic (FGF2) Progesterone were purchased from Sigma-Aldrich. OPSS-PEG-SVA (3400 Da) ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentration of total protein in CFE with recombinant Scaf19LKT was determined by Bradford reagent (Sigma-Aldrich, USA), CFE was diluted 1,000-times in 0.1 M sodium carbonate coating buffer (pH 9.0) ...
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Immunology 2020Quote: ... Transduced CD8+ T cells were expanded in the presence of 10 U/ml recombinant human IL-2 (Sigma) Human CD8+ T cells were purified from donor PBMCs (Cambridge Bioscience or NHSBT ...
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bioRxiv - Cell Biology 2020Quote: ... the cell medium was replaced with DMEM containing 0.2% FBS and 300ng/mL IGF-1 (human recombinant, Sigma) for 3 hours ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
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bioRxiv - Biochemistry 2022Quote: ... and ∼1 μg recombinant GST or GST-SMN in freshly made 25 mM bis tris propane (Sigma B6755) pH 6.8 buffer with 1 M NaCl and 0.05% NP-40 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells that were cultured in 0.5 % FBS were stimulated with hEGF (recombinant EGF) (Sigma Alrdich, Catalog No: E9644) in concentrations (10 and 30 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... except for the control group for which recombinant fusion proteins were mixed with incomplete Freund adjuvant (IFA, Sigma). Blood samples were collected from the submandibular vein at different time points for more than 6 months and sera were prepared and analysed for the presence of specific antibodies by ELISA as described below ...
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bioRxiv - Immunology 2022Quote: ... a control group received injection of recombinant fusion proteins (3.8 μM) mixed with complete Freund adjuvant (CFA, Sigma) in 50 μl of PBS ...