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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for Recombinant Mouse Icam2 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... V5-tagged protein was precipitated with 20 μl anti-V5 beads (Anti-V5 Agarose Affinity Gel, Cat.- No. A7345, Sigma-Aldrich) rotating on a wheel for 2 h at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... resolved by SDS-PAGE and immunoblotted for the presence of the HA- tagged effector using α-HA antibody (H9658; Sigma-Aldrich) and α-HA-HRP (3F10 ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary substrate was horse radish peroxidase tagged anti-rabbit secondary antibody and anti-goat secondary antibody (1:2500, EMD Millipore). The bands were detected using a chemiluminescent substrate (SuperSignal West Pico ...
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bioRxiv - Cell Biology 2024Quote: ... or BSA (as a control) were fluorescently tagged with a 568nm label using Mix-N-Stain CF 568 kit (Millipore Sigma). Unincorporated label was removed by a size filtration spin column and incorporation of the tag was checked by SDS-PAGE ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CHO cells were stably transfected with a SNAP-tagged human D2SR (short isoform) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 2 mM glutamine (Sigma-Aldrich, Poole, UK) and supplemented with 10% fetal calf serum (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... lysates corresponding to 1 OD600 of cells were analyzed by SDS-PAGE and immunoblotted with either anti-c-Myc (9E10, Covance Research) for Myc-tagged proteins or anti-peroxidase soluble complex antibody (Sigma, P2416) for TAP-tagged proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitates were washed three times with RIPA buffer and Flag-tagged proteins were eluted by two consecutive elution steps with 400 µg/ml Flag-peptide (Sigma-Aldrich) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... MBP- and GST-tagged proteins were purified by gel filtration on a Superdex 200 column (Sigma-Aldrich Cat# GE17-5175-01) and concentrated using a 30K Amicon filter.
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bioRxiv - Biochemistry 2023Quote: Hexahistidine tagged hMDC1 BRCT (residues: 1891 – 2089) was cloned into pET47b vector and expressed in Rosetta™ 2(DE3) cell lines (Novagen). Cells in 1L Luria Broth (LB ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-PA-tagged antibody conjugated with a PLUS oligonucleotide was prepared using the Duolink In Situ Probemaker PLUS (DUO92009; Sigma-Aldrich). The specimens were washed twice with wash buffer A and were subsequently incubated with Duolink ligation solution for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged Af1521 macrodomain beads were produced in-house using BL21(DE3) bacteria and coupled to glutathione Sepharose 4B beads (Sigma-Aldrich), essentially as previously described ...
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bioRxiv - Biochemistry 2024Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma, UFC501024) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated with HRP-tagged secondary antibodies and protein bands were detected using ECL Prime western blotting system (Catalog No. GERPN2232, Millipore Sigma). Protein band density was measured using ImageJ 1.52a ...
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bioRxiv - Molecular Biology 2024Quote: pDest17 plasmids containing an N-terminal 6x-His tag and Flag-tagged ARH3 wildtype or H182R were transformed into Rosetta (DE3)pLysS Competent Cells (Sigma, #70956). An initial 5 mL culture was grown overnight in Lennox LB broth supplemented with 100 μg/mL of ampicillin and 25 μg/mL of chloramphenicol at 37 °C while shaking at 200 rpm ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with 1/200 diluted Goat Anti human IgG Fc-FITC antibody (Cat: F9512, Sigma) for 45 min and analyzed using flow cytometry (CytoFLEX ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS and human fibroblast growth factor-2 (5 ng/mL, Sigma-Aldrich, St Louis, MO, USA) at 37 °C ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 10% FCS + 1% P/S + 0.1 μM phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich) + 1μM Ionomycin (Sigma Alrich ...
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bioRxiv - Cell Biology 2019Quote: ... Permeabilization and blocking of samples was performed in blocking solution (10% FCS, 0.3% Saponin (47036, Sigma-Aldrich) in PBS 1x ...
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bioRxiv - Immunology 2019Quote: ... in FACS buffer (PBS supplemented with 2% FCS and 2 mM (Ethylenedinitrilo)tetraacetic acid (EDTA) (Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were then washed 8 times and avidin-peroxidase (2.5 μg/mL in PBS / 10 % FCS. Sigma) was added for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... a 15-minute chase was performed with normal medium (0.5% FCS) supplemented with 10 µM thymidine (Sigma) to remove unincorporated EdU and cells were fixed and permeabilized with 3.7% FA and 0.5% Triton X-100 for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded in FluroDishes (World Precision) and imaged in DMEM supplemented with 10% FCS (Sigma-Aldrich), 100U/ml penicillin and 100μg/ml puromycin (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... the spinal cord was placed in a plexiglass tube and immersed in fluorinert (FC-40 Sigma Aldrich) to reduce background signal ...
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bioRxiv - Microbiology 2021Quote: ... Procyclic forms (PCF; strain 427) were cultured in modified SDM-79 with 10 % heat-inactivated FCS (Sigma) at 27°C ...
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bioRxiv - Bioengineering 2021Quote: ... and binding was detected with an anti-human IgG (Fc specific) (1:5000 dilution, Sigma-Aldrich, A0170) or anti-FLAG (1:1000 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... A trapped bead was manually manipulated by a motorized XY stage (BIOS-225T and FC-101G, Sigma), and contact of the bead with the cilium during oscillation for 1.5 h was monitored by ciliary GCaMP6 or mCherry fluorescence imaging or bright-field imaging of the bead ...
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bioRxiv - Cancer Biology 2019Quote: ... one cells have adhered remove culture medium and replace with diluted lentiviral media (500µl lentiviral media, 1.5ml DMEM, 10% FCS, 5% PenStrep, 5-10µg Polybrene (Sigma)) ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and resuspended in RPMI supplemented with 10% FCS and 1% penicillin streptomycin (Sigma Aldrich) (complete media ...
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bioRxiv - Pathology 2020Quote: ... stimulated for 24 hours in 10% FCS DMEM with LPS (1 µg/ml, Salmonella Minnesota; Sigma-Aldrich), BLP (Pam3Cys-Ser-(Lys)4 ...
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bioRxiv - Systems Biology 2019Quote: ... Subsequently, cells were washed two times with a washing solution (5% FCS, 0.05% BSA (Sigma-Aldrich; A2058), 0.02% NaN3(Carl Roth GmbH & Co ...
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bioRxiv - Developmental Biology 2021Quote: ... the embryos were harvested in sterile medium (10% FCS (Hyclone) and 1% pen/strep in PBS (Sigma)) ...
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bioRxiv - Immunology 2021Quote: ... Medium was replaced every 3 days thereafter with fresh R+ containing FCS (10% v/v; Sigma-Aldrich). Antigen-specific CD8+ T cells were characterized via flow cytometry on day 10.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Coding region of RBD-Fc was subcloned into a modified pCGS3 (Merck/formally known as Sigma-Aldrich) for glutamine selection in CHOZN GS-/- cells ...
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bioRxiv - Immunology 2022Quote: ... 1*105 cells in 200μl/well complete T-cell medium (RPMI1640 supplemented with 10% FCS (Sigma/Biowest), antibiotics [PenStrep] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... including Phosphate Buffered Saline (PBS) and Fetal Calf Serum (FCS) were purchased from Sigma Aldrich (Gillingham, UK), except for blastocidin which was obtained from Gibco™ (MA ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM glutamine and 10% dialyzed FCS after addition of 25 mM of either 12C-glucose (Sigma) or U-13C-glucose (Cambridge Isotopes ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% (v/v) heat-inactived fetal calf serum (FCS, Sigma-Aldrich, Saint Louis, MO, USA), 400 µg/ml G418 (Carl Roth ...
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bioRxiv - Immunology 2023Quote: ... density centrifugation and cryopreserved in heat-inactivated foetal calf serum (FCS) supplemented with 10% DMSO (Sigma-Aldrich) in liquid nitrogen ...
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bioRxiv - Immunology 2022Quote: ... 7 – 13×106 cells were washed in PBS with 2% FCS before incubation in 50nM dasatinib (Sigma) at 37°C for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Antibodies and dyes used in this study were: Anti-Human IgG Fc specific (Millipore Sigma I2136-1ML), Anti-6X His tag (Abcam ab9108) ...
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bioRxiv - Molecular Biology 2021Quote: ... PHM005 wild type and recombinant strains were grown in Marine Broth (MB) Difco 2216 (Sigma-Aldrich) or Marine Agar (MA ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1000U/ml ESGRO® recombinant mice leukemia inhibitory factor (LIF, Millipore, ESG1107, stock 107U/ml).
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bioRxiv - Cancer Biology 2019Quote: The uncharged polystyrene 96-well plate were coated with different recombinant proteins-Collagen IV (Merck Millipore), P-Selectin ...
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bioRxiv - Cell Biology 2020Quote: ... Solubilized recombinant FL-CatSper1 and ND-CatSper1 were pulled-down with anti-HA agarose (EMD Millipore) for 1 hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... Immunoassay plates were coated with 2.5 μg/ml recombinant angiotensin converting enzyme-2 (ACE2, Sigma Aldrich). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35 days ...
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bioRxiv - Molecular Biology 2021Quote: ... or recombinant leptin (1 mg/kg/d dissolved in dimethyl-sulfoxide) (L 3772 Sigma Chemical Co.), or mdivi-1 (50 mg/kg/d dissolved in dimethyl-sulfoxide ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant PRDX6 was purified by gel filtration chromatography on a Superdex 200 16/600 column (Sigma) equilibrated with 20mM HEPES at pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... were treated with or without 25-100 U/mL of human recombinant IFN-α (Sigma-Aldrich) for 4-12 hours after which total RNA was isolated and subjected to qRT-PCR in order to measure expression of ISGs.
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bioRxiv - Neuroscience 2022Quote: Fixation was performed following 24 hrs of recombinant protein treatment using 4 % PFA / 4% Sucrose (Sigma) in 1x PBS at RT for 15 min ...