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Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for Recombinant Mouse Icam2 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Sorted single cells were collected in ADF plus 10% FCS and 9 µM Rock inhibitor (Y-27632, Sigma) to prevent anoikis ...
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bioRxiv - Immunology 2019Quote: ... A subset of mice was treated with IP with recombinant DNase I (20 mg/kg, Sigma-Aldrich) 2 hours after LPS injection24 ...
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bioRxiv - Microbiology 2021Quote: ... enzymatic activity of recombinant CynD was measured at pH 8.0 using the Ammonia Assay Kit (Sigma-Aldrich). A concentration of 500 nM of CynD was used in all reactions with the following cyanide concentrations ...
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bioRxiv - Microbiology 2021Quote: ... Digestion of extended E34TSP was done according to the recombinant Enterokinase user protocol TB150 Rev.C 0107(Novagen). The extent of proteolytic activity on our TSP was calibrated via SDS PAGE analysis.
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bioRxiv - Biochemistry 2022Quote: ... Recombinant MejAgo protein expression and Ni-affinity chromatography purification procedures were performed according to user manual(Novagen). In perticular ...
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bioRxiv - Microbiology 2022Quote: ... and the recombinant protein expression was induced with 0.5 mM isopropyl-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich). The bacteria were incubated at 18°C for additional 18 hours ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with HKSdaFREE and HKGE (AvantBio) and 5 ng/mL human recombinant animal-free KGF (Sigma-Aldrich), 1.64 mM CaCl2 and 50 ug/mL ascorbic acid ...
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bioRxiv - Microbiology 2021Quote: ... Viral titers were calculated from a standard curve generated using recombinant reverse transcriptase (Millipore catalog no. 382129).
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Bioengineering 2019Quote: ... Recombinant human BMP-2 (Pfizer) was reconstituted in a 0.1% solution of rat serum albumin (Sigma-Aldrich) and 4 mM HCl and mixed with alginate ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Sf9 cells were transfected with recombinant bacmid DNA using Escort™ IV Transfection Reagent (Sigma, Cat # L3287) in 6 well plates ...
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bioRxiv - Plant Biology 2021Quote: GST and Recombinant Virp1-GST proteins were expressed in Escherichia coli Rosetta strain (EMD Millipore, Burlington, MA). Cells were grown overnight at 37°C in LB media supplied with ampicillin (0.1 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Eluted recombinant proteins were concentrated in Amicon Ultra-4 centrifugal filter units with 30-kDa cutoff (Millipore), concentrations were determined using the DC BCA protein assay (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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bioRxiv - Systems Biology 2023Quote: ... CD8+ T-cell cultures were supplemented with 120 IU/ml human recombinant IL-2 (Sigma Aldrich, USA). T-cells were aliquoted into five samples with 1.5 x 106 cells in each condition to obtain cells at five different time points (6 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant protein was then eluted in lysis buffer containing 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich) for 1 hr with rotation at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 400 µl of solution of recombinant TnC (Supplementary Materials and Methods) or purified human TnC (Merck Millipore) (15 µg/ml in 0.01% PBS-Tween ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Neuroscience 2024Quote: The inhibitory activities of 5–7 and all the reference compounds were determined using modified Ellman’s method [25,42,43] against human recombinant AChE (hAChE, E.C. 3.1.1.7, purchased from Sigma-Aldrich, Prague, Czech Republic) and human plasmatic BChE (hBChE ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human/mouse SMA-Cy3 (1:200, #C6198, Sigma Aldrich), rat anti-mouse CD35 (1:100 ...
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bioRxiv - Biochemistry 2020Quote: Mouse anti-FLAG M1 antibody and mouse anti-Sulfotyrosine antibody (Sigma) were used to detect the purified wild type C5aR and C5aRΔTyr with Y11F and F14F mutations respectively in the western blotting assays.
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bioRxiv - Neuroscience 2020Quote: ... and mouse anti-β-actin (Sigma, mouse monoclonal antibody, 1:5000). After incubation with HRP-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... monoclonal mouse anti-mouse GFAP (1:2000; Sigma G-A-5), and monoclonal rat anti-mouse IL-6 (1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... and mouse anti-β-actin (Sigma, mouse monoclonal antibody, 1:5000). After incubation with HRP-conjugated secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... Mouse Monoclonal anti-mouse Glutamin Synthetase (GS-6, 1:250, Millipore), Rabbit Polyclonal anti-SDS (PA5-58704) ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-NeuN (mouse monoclonal, 1:400, Millipore; catalog #MAB377, RRID:AB_2298772). This step was followed by incubation with secondary antibodies for 2h at RT to visualize primary antibodies ...
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bioRxiv - Genomics 2020Quote: ... and ESC lines stably expressing 3xHA-tagged open reading frames were cultured in the presence of 1 μg/mL puromycin (Sigma-Aldrich, Cat# P8833).
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bioRxiv - Neuroscience 2019Quote: Mouse cortical or hippocampal neurons expressing extracellular HA-tagged Nrxn1α for 30 hr were either treated with 20 μM Dynasore (Sigma-Aldrich, Cat #D7693) or DMSO ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were induced to express the indicated BioID2-tagged proteins by the addition of 0.2 μg/ml doxycycline (Sigma-Aldrich, St. Louis, MO) for 16 hours ...
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bioRxiv - Microbiology 2022Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection and filtered through 0.22μm PES membrane filters (Nalgene) ...
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bioRxiv - Biochemistry 2020Quote: ... RPS2-Flag tagged 40S ribosomes were immuno-precipitated by addition of 50 µL washed anti-Flag M2 affinity agarose gel (Sigma Aldrich, A2220-5mL) and 5 µL RNasin Plus (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... The overexpressed 3X FLAG-tagged cyclin-Cdk1 complexes were then purified by immunoaffinity chromatography using ANTI-FLAG M2 affinity agarose beads (Sigma-Aldrich Cat #A2220) and eluted with 0.2 mg/mL 3X FLAG peptide (Sigma-Aldrich Cat #F4799) ...
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bioRxiv - Pathology 2020Quote: ... Expression of human COMP in RCS cells was validated by Western Blot with anti-Flag antibody to recognize the tagged COMP (1:5000 F7425 rabbit polyclonal from Sigma, Saint Louis, MO). DOX was used for 4 days to induce COMP expression in RCS cells ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were blocked and incubated with specific antibodies: HA-tagged spike protein was detected with anti-HA antibody (H9658, Sigma-Aldrich, 1:10,000), hACE2 was detected with anti-ACE2 antibody (sc-390851 ...
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bioRxiv - Microbiology 2021Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection and filtered through 0.22 μm PES membrane filters (Nalgene) ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated with primary antibody Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit for detection of TAP-tagged proteins (Sigma-Aldrich, P1291-500UL) 1:1000 dilution in 5 % skimmed milk/PBST overnight at 4 °C followed by washing in 0.1%Tween/TBS ...
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bioRxiv - Biochemistry 2024Quote: ... adhered cells were washed with PBS and treated with cMyc-tagged CK2α or ZHER2-CK2α (1 μM) in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). Cells were incubated at 37 °C for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... cells were cultured for at least six days in RPMI 1640 Medium supplemented with 10% dialyzed FCS (Sigma Aldrich), containing either “heavy” lysine (40 g/l ...
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bioRxiv - Biophysics 2020Quote: ... Cytochalasin D (from Zygosporium masonii) as well as fetal calf serum (FCS) were from Sigma Aldrich (Merck KGaA, Germany). DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) ...
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bioRxiv - Molecular Biology 2021Quote: ... The HE-Fc containing fractions were dialyzed in PBS and concentrated using 30kD Amicon Ultra-15 filters (EMD Millipore). Purified proteins were observed by SDS gel migration as well as anti-human IgG Fc probing by Western ...
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bioRxiv - Developmental Biology 2019Quote: ... media was replaced with DMEM/F12 containing 100ng/ml Activin A and 0.2% foetal calf serum (FCS, Sigma-Aldrich) for a further 24 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were grown in DMEM (gibco) with 1 g/mL glucose (MCEC) or 4.5 g/mL (HUVEC, HeLa, MCF-7) containing 10% FCS (Sigma), 1% penicillin (10000 Units/ml ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were seeded at 1E6 cells/ml in MEM-Alpha (Biological Industries) supplemented with 10% Heat Inactivated FCS (Sigma) and 50IU rhIL-2 (Peprotech).
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bioRxiv - Cancer Biology 2020Quote: ... MDA-MB-231 cells were cultured in DMEM medium (Pan-Biotech, Germany) supplemented with 10% FCS (Sigma-Aldrich, Germany), 2 mM L-glutamine (Pan-Biotech ...
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bioRxiv - Biophysics 2020Quote: All used BMPs and extracellular domains (ECD) of the BMPR-FC chimeras were bought from Sigma Aldrich (Missouri, USA) and R&D systems (Minnesota ...
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bioRxiv - Cell Biology 2020Quote: ... the colon pieces were digested in RPMI-1640 medium containing 20% FCS and 100CU/mL collagenase IV (Sigma-Aldrich). Single cells were separated from the remaining tissue by filtration through 40 µm cell strainers and washed with RPMI-1640 thereafter ...