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Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 10 μM 1-[2-[[(Diphenylmethylene)imino]oxy]ethyl]-1,2,5,6-tetrahydro-3-pyridinecarboxylic acid hydrochloride hydrochloride (NO711) (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... media were aspirated and cells viability was determined by addition of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma) at a final concentration of 0.5 mg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was resuspended and incubated for 30 min at 4 °C in buffer B (10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 0.5% IGEPAL CA-630, 10% Glycerol, 1 mM DTT, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail) ...
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bioRxiv - Molecular Biology 2022Quote: Cell growth was monitored by cell counting and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using MTT cell growth assay kit (#CT02, Millipore). FL or ΔN-Drosha cells (1×105 ...
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bioRxiv - Biochemistry 2022Quote: ... D-glucose N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich, USA ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS and 10% 5mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent (SIGMA) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... we delivered the Iκ-kinase inhibitor [5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide (TPCA-1; Sigma-Aldrich CAS 507475-17-4 ...
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bioRxiv - Neuroscience 2022Quote: ... or MG132 using a MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-based in vitro toxicology kit (TOX1-1KT, Sigma). 10 µl MTT solution was applied to cells at the end point of the experiment and incubated for 4 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were incubated overnight from 4 dpf in 3 ml 10 mM 5-Bromo-2′-deoxyuridine (B5002, Sigma) with 1% DMSO for 17 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... The MTT [3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyl-tetrazolium Bromide] dye (M2128) was obtained from Sigma Aldrich. Triple distilled water (Millipore ...
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bioRxiv - Developmental Biology 2023Quote: ... 2-3 synthetic gRNAs per gene targeting exonic regions were designed using CCTop54 and ordered from Sigma-Aldrich (spyCas9 sgRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were counterstained after 3 washes of PBS with 0.3 μg/mL 4,6-diamidino-2-phenylindole (Sigma-Aldrich). Images including Z-stacks were acquired on a Leica SP8 gSTED with an objective with x63 magnification ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined after 72 hours using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (#M5655, Sigma) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 3 PBS washes were performed and blocking was done with 2% Bovine Serum Albumin (Sigma Aldrich,#A7906-100G) and 5% goat serum (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The MTT [3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyl-tetrazolium Bromide] dye (M2128) was obtained from Sigma Aldrich. Triple distilled water (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... After 24 hrs media/inhibitor was aspirated and replaced with 20 μl of 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) (Sigma) and incubated at 37 °C in 5% CO2 for 3 hrs ...
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bioRxiv - Neuroscience 2023Quote: ... slides were cleared in Xylene (2 × 3 min) and coverslipped with DPX mounting medium (Cat# 44581, Sigma-Aldrich). Sections were photographed with an epifluorescence microscope (Model BX51 ...
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bioRxiv - Microbiology 2023Quote: ... and the cells were washed 3 times in PBS before addition of 2% Triton X– 100 (Sigma-Aldrich) PBS solution to lyse the cells for enumeration of the intracellular bacteria ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Biochemistry 2023Quote: ... and phosphatase inhibitors (prepared in-house according to Phosphatase inhibitor cocktail 1, 2 and 3 from Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2024Quote: ... spheroids were incubated with 0.5 mg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich) for 2-3 h until fully stained ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 mg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, St Louis, MO, USA) was added to selected wells and cells were incubated at 37°C for 4h ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μL MTT (5 mg/ml tetrazolium salt 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, Sigma-Aldrich) was added to each well and kept in a dark for 4 hours at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The db/db mice were treated with Ang-(1-7) by subcutaneous infusion of Ang-(1-7) (Sigma-Aldrich, St. Louis, MO) (100 ng/kg/min ...
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bioRxiv - Neuroscience 2021Quote: Cortical synaptosomes were incubated at 35 °C with 450 rpm in PBS with 2 mM ATP (Sigma-Aldrich), 125 mM HEPES ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cultured overnight at 37 °C in 5% CO2 in neurobasal medium supplemented with 2% B27 (Sigma-Aldrich) and containing 50 ng/mL GDNF (PeproTech ...
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bioRxiv - Cell Biology 2019Quote: ... control and DnajB6 silenced cells were incubated for 2 hours at 37°C with 2μM STLC (Sigma, 164739), fixed in −20°C methanol and processed for IF using the DM1A or anti-tubulin-β antibodies.
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bioRxiv - Microbiology 2020Quote: ... HIV-GFP produced with p8.91 or p8.2 used in Figure 1 were DNase treated for 2 hours at 37°C (DNaseI, Sigma) prior to ultracentrifugation ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with 0.1 mL serially diluted supernatant containing SARS-CoV-2 for 30 minutes at 37°C and 5% CO2 before being left in 1.5mL overlay (DMEM, 2% FBS, 1.2% cellulose microcrystalline (Sigma)) at 37°C and 5% CO2 for 72 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... minced and digested for 90 minutes at 37°C in 2 mg/ml collagenase type I (Sigma – SCR103) in HBSS containing 2mM CaCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... All embryos were maintained in Ringer’s solution at 28.5°C and treated with 0.003% 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich) from 8 hpf.
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bioRxiv - Cancer Biology 2022Quote: ... and subsequently heated at 100°C for 10 min followed by the addition of 2-mercaptoethanol (Sigma-Aldrich). Proteins were separated by acrylamide gel electrophoresis and blotted according to standard protocols ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was incubated for 1.5-2 h at 4 °C with Anti-FLAG M2 affinity gel (Sigma) while gently mixing ...
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bioRxiv - Microbiology 2019Quote: ... at 4°C and resuspended in 2 ml of NP40 lysis buffer (1× CellLytic B (Sigma, MO, USA), 50 U DNAse I (Fermentas ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were incubated 2 minutes at 4°C with 0.1 mg/ml propidium iodide (Cat. P4170, Sigma Aldrich) and fluorescence pulses were detected using 585/40 nm collection filter ...
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bioRxiv - Immunology 2021Quote: ... DN3/4-enriched thymocytes were transduced with fresh viral supernatant via spinfection for 2 hours at 2300 rpm at 30°C with 6.7µg/mL polybrene (Millipore). Following spinfection cells were transferred to culture on OP9-DL1 stromal cells for overnight culture ...
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bioRxiv - Microbiology 2020Quote: ... lysogens were grown to 0.1 – 0.2 OD600 and 2 μg ml-1 mitomycin C (Sigma, from Streptomyces caespitosus) added to induce resident prophages ...
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bioRxiv - Molecular Biology 2023Quote: ... Rie1-3V5 was IPed at 4°C 2 h using 20 µL of anti-V5-agarose slurry (Sigma). After incubation ...
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bioRxiv - Immunology 2023Quote: ... Surface staining was performed for 30 min at 4 °C in PBS supplemented with 2% FCS (Sigma-Aldrich) and 0.01% azide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... for 2 hours at 4 °C and then incubated with anti-Flag M2 affinity gel (Millipore Sigma # A2220) for 1.5 hours at 4 °C under continuous rotation ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated for 2 hrs at 4 °C with Protein A/G Mix Magnetic Beads (Cat# LSKMAGAG10, Millipore). After washing with dilution buffer ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... 5-7 IU of PMSG (Millipore #367222/BioVendor #RP1782721000) was injected (IP ...
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bioRxiv - Developmental Biology 2021Quote: ... 7-AAD (Sigma, 10 µg ml−1, 1:100) or Zombie Aqua (Biolegend ...
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bioRxiv - Synthetic Biology 2022Quote: ... 7 or 10 mM of lysine (Sigma Aldrich, L5501) or glutamine (Serva ...
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bioRxiv - Biochemistry 2021Quote: ... 7 μl of 500x Sypro Orange (Merk Sigma-Aldrich,) and 63 μl of ultrapure H2O were prepared ...
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bioRxiv - Plant Biology 2019Quote: ... 7-day old seedlings were treated with MG132 (Sigma) for 16 hrs in a liquid medium containing ½ MS salt.
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bioRxiv - Bioengineering 2021Quote: ... and 0.5 g of MgSO4 ·7 H2O (Sigma-Aldrich) in milli-Q water ...
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bioRxiv - Cancer Biology 2019Quote: ... Huh-7 12 (HUH7) were purchased from Sigma-Aldrich. Both cell lines were grown in RPMI 1640 supplemented with 10% FBS and antibiotics at 37°C in an atmosphere of 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Y27632 was purchased from Abcam (ab120129) and ML-7 was purchased from Sigma (475880).