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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... RPMI 1640 complemented with indicated inhibitors was replaced daily for 3 days before overnight fixation with formalin at 4°C (Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... 5 % CO2 at 37 °C were seeded on Seahorse microplates pre-coated with 3 μg/mL collagen I (C9791, Sigma Aldrich) in 0.1 M acetic acid and allowed to adhere for at least 12 h ...
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bioRxiv - Evolutionary Biology 2021Quote: An overnight culture of Bacillus thuringiensis Berliner (ATCC 55177) was prepared from a glycerol stock and grown at 30°C in Nutrient Broth #3 (NB, Sigma Aldrich). An aliquot of overnight culture was brought to an OD of 0.5 in fresh NB ...
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bioRxiv - Biochemistry 2022Quote: ... for 24 hours and serum starved with OptiMEM for 48 hours prior to fixation with 3% PFA/PBS for 3 minutes in room temperature followed by a 5 min incubation at −20 °C with pre-cooled methanol (Sigma Aldrich). Fixed cells were blocked with 3% BSA (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... VTA brain sections were incubated for 1 hour at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... VTA brain sections were incubated for 1 hour at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Cell Biology 2023Quote: ... The AAV was concentrated by centrifugation at 2000g for 3-5min at 4°C using an 100k Amicon Ultra Centrifugal Filter Unit (Millipore #UFC810024). After centrifugation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for 3 hours and then incubated overnight at 4°C in block with primary antibodies: mouse anti-acetylated tubulin (Sigma, T6793) at 1:250 or rabbit anti-phosphorylated Smad1/5/8 (Cell Signaling ...
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bioRxiv - Physiology 2023Quote: ... and then incubated overnight at 4°C in primary antibodies diluted in 50% blocking buffer / 50% Tris buffered saline (bioWORLD #42020056–3) + 0.1% TWEEN (Sigma-Aldrich #P9416) (TBS-T) ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.74 mmol of 3 or 4 azidoacids were stirred for 10 minutes at 40°C in minimal dimethylformamide (Sigma-Aldrich, 319937). The solution was then added with 0.74 mmol of DCC (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... solution for 3 hours at 4°C under gentle agitation and then washed it three times in TBS/Tween20 (TBST) buffer (Sigma Aldrich). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... cleared biopsies were blocked for 3–4 h at room temperature (RT) or overnight at 4°C in blocking buffer (PBS (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... single-cell suspensions of colon and spleen were stimulated for 3 h at 37°C with 50 ng/ml PMA (Sigma-Aldrich) and 1 μM ionomycin (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 3-4 h at 4 °C with 15-20 μL of ANTI-FLAG® M2 Affinity Gel (Sigma) and washed 3-4 times with Co-IP wash buffer containing 0.1% Triton-100 but without Phosphatase Inhibitor Cocktails and Protease Inhibitor Cocktail ...
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bioRxiv - Genetics 2023Quote: ... at 4°C for 1 h followed by overnight incubation with 3 μL of the following antibodies: H3K4me2 (Millipore 07-030), H3K9me3 (Active Motif 39161) ...
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bioRxiv - Immunology 2023Quote: Cells obtained by crawl-out were incubated for 24h at 37°C with the phosphatidylinositol-3-kinase inhibitor wortmannin or the carnitine palmitoyltransferase-1 inhibitor etomoxir (both from Sigma-Aldrich), at 10 and 200μM respectively.
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bioRxiv - Bioengineering 2023Quote: ... the samples were first homogenized and hydrolyzed in concentrated hydrochloric acid (HCl) at 120°C for 3 hours before being quantified using hydroxyproline assay kit (Sigma-Aldrich). A microplate reader (Promega GloMax ...
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bioRxiv - Cancer Biology 2021Quote: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma-Aldrich (L’Isle-d’Abeau-Chesnes, France). Antibodies against phospho-IκBα Ser32 (#2859) ...
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bioRxiv - Neuroscience 2021Quote: Prior to training rats were randomly assigned to receive either sham surgery or intracranial bilateral lesions to the region of interest under 2-3% isoflurane anaesthesia with local application of bupivacaine (2mg/kg s.c. at 0.8ml/kg; Sigma) at the incision site ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in aCSF) or with 2µL Trpv4 antagonist (HC-067047, Sigma 2 µg diluted in aCSF-DMSO ∼3 %). The treatment was counterbalanced between trials ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in aCSF) or with 2µL Trpv4 antagonist (HC-067047, Sigma 2 µg diluted in aCSF-DMSO ∼3 %).
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bioRxiv - Immunology 2019Quote: ... 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) was purchased from Sigma-Aldrich (Saint Louis, MO, USA) and GeneJuice® Transfection Reagent (Novagen ...
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bioRxiv - Neuroscience 2020Quote: ... SYSY), vesicular glutamate transporter 2 (VGLUT2; 1:1,000, SYSY) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, 1:50,000, Millipore) at 4 °C for 16 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Metabolic activity was measured by adding 10 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 10 mg/ml, Sigma). After four hours of incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.1% SDS with cOMPLETE™ Mini protease inhibitor cocktail (#11836153001) and phosphatase inhibitor cocktail 2 and 3 (Sigma)) ...
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bioRxiv - Neuroscience 2020Quote: ... 6–8-week-old C57BL/6N mice were anesthetized by inhalation of isoflurane (3–4%) or intraperitoneal injection of 2% Avertin solution (2,2,2-tribromoethyl alcohol dissolved in Tert-amylalcohol (Sigma)) dissolved in saline ...
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bioRxiv - Cell Biology 2020Quote: ... or erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA; 100μM, E114-25MG, Sigma-Aldrich, Saint Louis, MI, USA) prior to fixation with 2% PFA (28906 ...
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bioRxiv - Plant Biology 2021Quote: ... The worms were washed 2–3 times with PBS-T and then stained with 0.125% thioflavin-S (Sigma) in 50% ethanol for 2 min and then de-stained sequentially in ethanol (50% ...
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bioRxiv - Microbiology 2021Quote: 5-aza-2’-deoxycytidine (5-AzadC, A3656) and epigallocatechin-3-gallate (EGCG, E4143) were purchased from Sigma Aldrich. Antibodies against CREB (sc-186) ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were neutralized with 3% ammonium hydroxide and extracted with 2 ml of water-saturated diethyl ether (Sigma), vortexed and centrifuged at 2,000 x g for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... This plasmid was transformed to Novagen’s Rosetta™ 2(DE3)pLysS competent cells (Millipore Sigma, Cat#71403-3) for rhFGF10 expression ...
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bioRxiv - Cancer Biology 2022Quote: ... 25mM Tris) supplemented with protease and phosphatase inhibitors (Protease inhibitor cocktail, Phosphatase inhibitor cocktail 2/3, Sigma Aldrich) for 5 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... 3 the cell culture media was replaced 1-2 hours before imaging with RPMI media (Sigma-Aldric R8755) supplemented with B27 ...
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bioRxiv - Microbiology 2022Quote: ... polymyxin B sulfate and 2-Heptyl-3-hydroxy-4(1H)-quinolone (PQS) (Sigma-Aldrich Corp., St. Louis, MO) were dissolved in water ...
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bioRxiv - Bioengineering 2022Quote: ... The activated membranes were immediately submerged in a solution of 2% bis[3-(trimethoxysilyl)propyl]amine (Sigma-Aldrich) in 1% water and isopropyl alcohol ...
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bioRxiv - Bioengineering 2022Quote: ... slides were stained in Phosphomolybdic Acid / Phosphotungstic Acid mix (Sigma-Aldrich, product number HT15-3 and HT15-2) for 7 minutes and in Aniline Blue Solution (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... Silicon chips were functionalized with a 2% (3-aminopropyl) triethoxysilane (APTES, 98%, Sigma-Aldrich, St. Louis, MO, USA) solution to attach complexes to the surface by interactions with free amine groups ...
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bioRxiv - Plant Biology 2021Quote: ... The full length coding sequence of AdACS1/2/3 and AdACO3/5 were inserted into pET-32a (Novagen) vector and then transferred into Escherichia coli strain BL21 (DE3) ...
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bioRxiv - Neuroscience 2021Quote: ... and at 3 days in-vitro (DIV) glial proliferation was inhibited with 2 μM cytosine arabinoside (Sigma, C6645).
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bioRxiv - Immunology 2021Quote: ... Subsequently cells were washed 3 times with PBS before being treated with 2% saponin (250 µL/well) (Sigma) in distilled water and incubated for 15 min at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately1-2 μL of endotoxin-free DNA (1-3 mg/ml) diluted in PBS/0.025% Fast Green (SIGMA) was injected into the lateral ventricles of the forebrain using heat-pulled glass micropipettes (Drummond) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cell viability was evaluated by using the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) cell proliferation assay (Sigma). Non-treated cells were used as control ...
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bioRxiv - Biophysics 2019Quote: ... cells were incubated with 0.5 mg/ml 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT, Sigma-Aldrich) for 2 hours at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The recording chamber was constantly perfused (2-3 ml/min) with aCSF and 100 μM picrotoxin (Sigma Aldrich) was added to block inhibitory synaptic transmission ...
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bioRxiv - Neuroscience 2019Quote: ... except that subjects received counterbalanced ICV infusions (3 uL) of either vehicle [2% DMSO (Sigma-Aldrich, D8418-50ML) + 10% 2-hydroxypropyl-β-cyclodextrin (Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... Coverslips were rinsed with distilled water and then incubated in 0.5% 3-Aminopropyltriethoxysilane (APTES) (Sigma, 919-30-2) in 95% ethanol ...
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bioRxiv - Immunology 2019Quote: ... 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) was obtained from Sigma-Aldrich (Saint Louis, MO, USA). FcR blocking reagent (BR ...
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bioRxiv - Genetics 2020Quote: ... cells were UV-irradiated (60 J m-2) through isopore polycarbonate membranes containing 3-μm-diameter pores (Millipore). For CPD immunodetection ...
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bioRxiv - Cell Biology 2020Quote: ... Both the CS and cantilevers were washed with acetone and functionalized using 2% (v/v) 3-aminopropyltriethoxysilane (Sigma) dissolved in acetone ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...