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Citations for Millipore Sigma :
1201 - 1250 of 1323 citations for Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... anti-p-PKC-δ-T505 (AP0776) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was submerged in secondary antibody solution (TBS-T + 5% BSA + 1:3000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody (Sigma-Aldrich) and incubated for two hours at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... This was followed by a 1 h incubation at room temperature with anti-mouse or anti-rabbit secondary antibodies conjugated with horseradish peroxidase (HRP; Sigma, A8924). Blots were developed using a Konica SRX101A X-Ray Film Processor.
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bioRxiv - Developmental Biology 2023Quote: ... Cell proliferation was determined with the iClick EdU Imaging Kit (ABP Biosciences) and apoptosis was measured using the ApopTag Peroxidase in Situ Apoptosis Detection Kit (Millipore Sigma), following the manufacturers’ protocols ...
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bioRxiv - Plant Biology 2023Quote: ... Anti-rabbit secondary antibody conjugated with horse radish peroxidase enzyme was used in the ratio of 1:10000 (Sigma Aldrich, USA) and blots were visualized using chemiluminescent substrate as described above.
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with TBS-T and incubated with 1:10000 goat anti-rabbit poly-HRP secondary antibody (Thermo, 32260) and 1:10,000 anti-β-actin-peroxidase antibody (Sigma, A3854) in 5% bovine serum album/TBS-T for 1 hr ...
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bioRxiv - Immunology 2023Quote: ... The wells were rinsed three times with PBS containing 0.05 % Tween 20 and horseradish-peroxidase-labeled rabbit anti-human or anti mouse IgG (100 μL/well; Sigma-Aldrich, Germany) was added to each well and incubated for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Bound NS5A antibody was detected after two washes with PBS-T by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse antibody (A4416, Sigma-Aldrich) diluted 1:300 in PBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein loading was determined using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000) from Sigma-Aldrich (A3854). Quantitation of western blot band intensity was done using Image J software.
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bioRxiv - Developmental Biology 2023Quote: ... the membrane was incubated for 1h at room temperature with the secondary antibody anti-mouse IgG peroxidase-conjugated (1/500; A5906, Sigma-Aldrich). The revelation was performed using Pierce™ ECL Plus Western Blotting Substrate (32312 ...
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bioRxiv - Biochemistry 2023Quote: Free and encapsulated DyP peroxidase (KpDyP and KpDyP_Enc) were purified and diluted to equimolar heme concentrations as determined via heme assay kit (Sigma-Aldrich, USA) per manufacturer protocol to analyze enzyme activity via ABTS assays ...
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bioRxiv - Genetics 2023Quote: ... The next morning membranes were washed once with blocking buffer for 5-10min and incubated with horseradish peroxidase-conjugated secondary antibodies (Sigma-Aldrich) diluted 1:10 000 in blocking buffer ...
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bioRxiv - Plant Biology 2024Quote: ... and incubated overnight in the same solution at 4°C with a 6000 X dilution of anti-HA-peroxidase antibodies (Sigma-Aldrich) in 0.5 % milk in TBS-Tween 0.1 % to reveal HA-tagged NIN proteins following chemiluminescence revelation using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... or incubations with species-specific biotinylated antibodies (Table S6; Vector) and then streptavidin–horseradish peroxidase (HRP) antibody (Vector; 1:200) before development with diaminobenzidine (Sigma-Aldrich). Counterstaining was performed using toluidine blue ...
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bioRxiv - Biochemistry 2024Quote: ... After incubation with horse-radish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit-HRP and goat anti-mouse-HRP, Sigma-Aldrich) at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were removed using a cotton swab and bone slices were incubated with 20 μg/ml peroxidase-conjugated wheat germ agglutinin in PBS (Sigma, 61767) for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma) in washing solution for 1h ...
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bioRxiv - Microbiology 2024Quote: ... α-PilT (66) (dilution 1:2000) and α-PilB (66) were used together with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (Sigma) as a secondary antibody (dilution 1:10000) ...
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bioRxiv - Cancer Biology 2024Quote: ... Beads were then resuspended in Laemmli buffer and boiled at 95 °C for 5 min prior to SDS-PAGE and immunoblot analysis using FLAG antibody covalently conjugated to horse radish peroxidase for detection of precipitated L22 (A8592, Sigma-Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
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bioRxiv - Developmental Biology 2021Quote: ... membranes were washed 3 times with TBS-T for 5 min each and incubated for 1 h with peroxidase-conjugated anti-rabbit secondary antibody (Sigma, cat # A0545) diluted 1:10,000 in 1 % BSA solution in TBS-T ...
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bioRxiv - Biochemistry 2019Quote: ... Blots were probed for 1 hour at room temperature with a horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (Sigma-Aldrich; A0545) diluted 1:10000 in the same buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Blots were probed for 1 hour at room temperature with a horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (Sigma-Aldrich; A0545) treated with enhanced chemiluminescence reagent (Thermo Fisher ...
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Multiple steps mediate ventricular layer attrition to form the adult mouse spinal cord central canalbioRxiv - Developmental Biology 2019Quote: ... TUNEL assay was performed on 20 μm thick cryosections using ApopTag® Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore, Cat. # S7100) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... prior to addition of conjugate diluent (PBS, 1% BSA) containing a 1:500 concentration of peanut lectin conjugated to horseradish peroxidase (PNA-HRPO, Sigma Aldrich, UK). Plates were then incubated for 2h in the dark ...
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bioRxiv - Cancer Biology 2019Quote: ... TUNEL staining was performed on deparaffinized sections to detect late-stage apoptotic cells using the ApopTag peroxidase in situ apoptosis detection kit (Millipore, Burlington, MA), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed using an ApopTag® In Situ Peroxidase Detection Kit (S7100, Merck Millipore, USA) according to the manufacturer’s recommendations and as previously described (36) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed a further three times in TBS-T prior to antibody detection using enhanced chemiluminescence horseradish peroxidase substrate detection kit (Millipore, Hertfordshire, UK). Imaging was undertaken using a G:BOX Chemi-XR5 (Syngene ...
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bioRxiv - Plant Biology 2020Quote: ... Detection was carried out by chemiluminescence assays after incubation with an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (Sigma or Santa Cruz).
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bioRxiv - Microbiology 2020Quote: ... which was un-exposed European pooled serum.23 Secondary detection was with Human IgG (H&L) Antibody Peroxidase Conjugated (Sigma – Aldrich, Germany), developed with Pierce™ TMB Substrate Kit (Thermo Fischer ...
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bioRxiv - Molecular Biology 2021Quote: ... The development was performed in an ImageQuant™ LAS 500 Ge Life Sciences apparatus (Piscataway, NJ, USA) using the appropriate substrate of peroxidase (Luminata™Forte, Merck Millipore). The reaction was conducted in the dark ...
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bioRxiv - Cancer Biology 2021Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed using the ApopTag® Peroxidase in situ Apoptosis Detection Kit (EMD Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Expression levels of Ib30 were accessed by western blotting with anti-HA-peroxidase conjugate (dilution-1:1000, Sigma-Aldrich; cat. no. 12013819001). Anti-β-actin antibody (dilution-1:2000 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The ratio of protein level or phosphorylation levele against control β-actin protein level (detected with a mouse monoclonal Anti-β-Actin-Peroxidase antibody (A3854 Sigma)) was normalized to untreated wt sample mean.
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bioRxiv - Plant Biology 2021Quote: ... Immunodetection was performed by incubating the immunoblot for 2 h with anti-mouse IgG conjugated with horseradish peroxidase (1:10,000 dilution; Millipore; Catalog # 12-349). Detection was performed using the SuperSignal West Dura Detection Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three more times in TBS-T before antibody detection using enhanced chemiluminescence horseradish peroxidase substrate detection kit (Millipore, Hertfordshire, UK). Imaging was conducted using a G:Box Chemi-XR5 (Syngene ...
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bioRxiv - Molecular Biology 2019Quote: Recombinant rcCBP2 was run on SDS-PAGE and subjected to western blotting using anti-hexa histidine HRP (horse radish peroxidase) conjugated antibodies (Sigma; 1:2000) to confirm identity of the purified protein.
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bioRxiv - Pathology 2019Quote: ... membranes were incubated for 30 min at room temperature in PBS-T containing 5% milk powder and secondary antibody: peroxidase-conjugated anti-mouse IgG (1:5000) (Sigma-Aldrich, A2304). The membranes were then washed in PBS-T ...
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bioRxiv - Microbiology 2019Quote: ... plates were incubated with horseradish peroxidase-labelled goat anti-mouse IgG antibody (Sigma-Aldrich, cat # AP124P, diluted 1:3000 in PBS-T) for 1 h ...
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bioRxiv - Developmental Biology 2019Quote: ... the membranes were washed 3x in TBS-T for 5 min each and incubated for 1 h with peroxidase-conjugated anti-rabbit secondary antibody (Sigma, cat # A0545) diluted 1:10,000 in TBS-T + 1% BSA solution ...
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bioRxiv - Microbiology 2020Quote: ... The primary antibody used for Western blot was a horseradish peroxidase conjugated goat anti-mouse IgG (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Physiology 2020Quote: ... TUNEL staining was performed using a protocol modified from the ApopTag peroxidase in situ apoptosis detection kit (Merck-Millipore, Cat no. S7100). Paraffin sections of adipose tissues were prepared as above ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed again 5 times with the washing buffer to discard unbound serum and 100 μl of horseradish peroxidase-conjugated goat anti-mouse IgG (Sigma-Aldrich, USA) diluted at 1:2000 in 1% BSA in washing buffer was added to each well followed by 1 hour incubation at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... conjugated anti-histidine was added and color developed by addition of and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St. Louis, MO, USA). Plates were read at an OD of 450 nm with a SpectraMax Plus plate reader (Molecular Devices ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were incubated for 30 minutes with a horse radish peroxidase (HRP)-conjugated sheep anti-DIG primary antibody (Sigma, catalog # 11207733910) diluted at 1/500 in Tris-NaCl blocking buffer (TNB ...
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bioRxiv - Microbiology 2022Quote: ... terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining of paraffin-embedded sagittal sections was performed using Apoptag Peroxidase In Situ Apoptosis Detection Kit (EMD Millipore, S7100), per the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Then membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase conjugated secondary antibodies (1:3000, Sigma Aldrich, St. Louis, MO) for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... conjugated with horseradish peroxidase (HRP), (KPL, MD, USA) was used as secondary antibody and detected by chemiluminescence substrate (Immobilon western, Millipore, CA, USA) then exposed to an X-ray film ...
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bioRxiv - Physiology 2022Quote: HaCaT and HaCaT DUOX1 knockout confluent cells on coverslips were washed with H-medium and treated with 1 μM thapsigargin in the presence or absence of 0.2 U/ml horse radish peroxidase and 27,5 μM biotinyl tyramide (Sigma Aldrich, Burlington, MA, USA) for 5 minutes at 37 °C ...