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Citations for Millipore Sigma :
1151 - 1200 of 1323 citations for Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with TBS-T (3 times for 10 min) and incubated with horseradish peroxidase-conjugated secondary antibodies anti-mouse (Millipore, #AP308P), or anti-rabbit (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed 3 times with washing buffer then 100 μL of diluted goat anti-mouse IgG (Fc specific)-Peroxidase antibody (#A0168; Sigma-aldrich) were added ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The following day plates were washed three times with TBS-T and incubated for 2 hours at room temperature with secondary antibody horseradish peroxidase-conjugated rabbit anti-horse IgG (Sigma A6917), diluted to 1 in 1,000 in PBS ...
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bioRxiv - Immunology 2022Quote: ... IgM or IgA secondary antibody conjugated to horseradish peroxidase (HRP) (Jackson ImmunoResearch 109-036-088, 109-035-129, and Sigma A0295) in blocking buffer at a 1:5,000 dilution (IgM and IgG ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were immunoblotted with HRP-conjugated Flag antibodies (Monoclonal ANTI-FLAG® M2-Peroxidase HRP antibody produced in mouse, A5892; Merck-Millipore), HRP-conjugated HA antibodies (12013819001 ...
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bioRxiv - Cell Biology 2020Quote: ... Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit antibody conjugated to peroxidase (Sigma Aldrich). For tagged proteins detection ...
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bioRxiv - Cell Biology 2020Quote: ... Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795, Sigma-Aldrich). The membranes were then developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... or anti-gL (rabbit polyclonal) antibodies and detected using anti-rabbit or anti-mouse secondary antibodies conjugated to horseradish peroxidase (Sigma-Aldrich) and Pierce ECL-Western blotting substrate (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... the immune peroxidase was detected using a Vectastain ELITE ABC kit (Vector) and visualized with a solution of diaminobenzidine (Sigma Aldrich) in the presence of 0.01% H2O2 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were then washed with TBST buffer (5 x 1 min) and probed with secondary goat anti-rabbit antibodies conjugated with peroxidase (1:1000, Sigma #A6154). Blots were washed as described above and detection was carried out using Amersham ECL Western Blotting Select Detection Reagent (GE Lifescences #RPN2235 ...
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bioRxiv - Microbiology 2021Quote: ... water was replaced by 100 μl of fresh Milli-Q water containing 20 μg/ml horseradish peroxidase (Sigma-Aldrich, Taufkirchen, Germany) and 20 μM L-012 (Wako Chemicals ...
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bioRxiv - Plant Biology 2021Quote: ... The PVDF membrane was then incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (diluted 1:10,000; Sigma-Aldrich, Stockholm, Sweden) for 1 h at room temperature followed by three washes with TBST and detection using a Agrisera ECL kit (catalogue no ...
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bioRxiv - Immunology 2021Quote: Detection of IgA spots was achieved by using anti-IgA-conjugated horseradish peroxidase and developed with 3-amino-9ethylcarbazole (Sigma-Aldrich). Color development was stopped by washing several times with water ...
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bioRxiv - Immunology 2020Quote: ... The IHC protocol mirrors the MIBI-TOF protocol with the addition of blocking endogenous peroxidase activity with 3% H2O2 (Sigma Aldrich) in ddH2O after epitope retrieval ...
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bioRxiv - Microbiology 2019Quote: ... Detection of FLAG-tagged proteins transferred onto a PVDF membrane was performed with monoclonal anti-FLAG M2-peroxidase (HRP) antibodies (Sigma-Aldrich) and Lumi-Light Western Blotting Substrate Kit (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: ... H-3300) for 30 min in a steamer and inactivation of endogenous hydrogen peroxidase activity with 10% H2O2 solution (Sigma, H1009), followed by blocking using 10% Normal goat serum (Jackson Immuno Research ...
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bioRxiv - Microbiology 2019Quote: ... alkaline phosphatase-conjugated (1:10,000) or goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated (1:10,000) secondary antibody (Sigma, St. Louis, MO). Immunoreactive bands were detected by the addition of NBT/BCIP (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Blotted proteins were detected with a monoclonal antibody that recognizes SARS-CoV-2 S1 (SinoBiological) and a secondary anti-rabbit horse radish peroxidase (HRP)-conjugated antibody (Sigma Aldrich). Blots were developed with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were resolved by tricine-SDS-PAGE and visualized by Western-blots probed by Ub-antibody Extravidin®-Peroxidase (Sigma #E2886). All experiments were conducted in triplicate (N=3).
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bioRxiv - Immunology 2021Quote: ... IgM or IgA secondary antibody conjugated to horseradish peroxidase (HRP) (Jackson Immuno Research 109-036-088 109-035-129 and Sigma A0295) in blocking buffer at a 1:5,000 dilution (IgM and IgG ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 90 min with either horse-radish peroxidase (HRP) conjugated goat anti-rabbit IgG (1:10000; EMD Millipore) or HRP conjugated goat anti-mouse IgG (1:10000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were subsequently incubated with the indicated primary antibodies followed by the appropriate horseradish peroxidase-labelled antibodies (1/10000, Sigma-Aldrich or GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: ... Plates were washed thrice with PBS-T followed by the addition of 20 μL per well of Streptavidin-Peroxidase polymer (Sigma #S2438) diluted in dilution buffer to 1.25 ng/μL ...
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bioRxiv - Microbiology 2020Quote: ... Peroxidase-conjugated secondary antibodies (Antgene, 1: 5000) were applied accordingly followed by image development with Chemiluminescent HRP Substrate Kit (Millipore Corporation).
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bioRxiv - Immunology 2021Quote: ... was used at a 1:5000 dilution (T84) or horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (A0545, Sigma-Aldrich) at 1:2000 dilution ...
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bioRxiv - Immunology 2020Quote: ... Mouse antibodies were detected by incubation with polyclonal goat anti-mouse IgG-horseradish peroxidase conjugate (Sigma-Aldrich, St. Louis, MO, USA). The plate was developed with TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... the wells were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) added to the wells ...
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bioRxiv - Immunology 2020Quote: ... The membrane was initially blocked with 5% skin milk with PBS buffer for 2 hours and after few PBS rinse the membrane was incubated with monoclonal Anti-polyHistidine−Peroxidase antibody produced in mouse (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... Wells were incubated with serial dilutions of the serum samples and the bound total IgG specific antibodies were detected by Peroxidase-conjugated Goat Anti-Mouse IgG (Sigma-Aldrich). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed in 1xTBS and then in BS before probing with anti-rabbit antibody conjugated to Horse Radish Peroxidase (HRP) (1:10,000 in BS, Sigma, goat, A9169) for 60 minutes at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were incubated at room temperature for 1 hour with horseradish peroxidase (HRP) conjugated goat anti-mouse (1:10:000 Sigma 4416) or donkey anti-rabbit (1:10,000 ...
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bioRxiv - Biochemistry 2022Quote: ... The following secondary antibodies diluted to 1:6000 in 3% milk and applied for two hours at room temperature: goat anti-Rabbit IgG peroxidase (Sigma #A0545) and goat anti-mouse IgG peroxidase (Sigma #A0168) ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then washed with PBS-T and incubated with anti-rabbit or anti-mouse peroxidase-labeled antibodies (1:10000, Sigma-Aldrich). After extensive washing with PBS-T ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (Jackson Immuno Research 109-036-088 109-035-129 and Sigma A0295) in blocking buffer at a 1:5,000 dilution ...
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bioRxiv - Plant Biology 2022Quote: ... W was replaced by 100 μL of reaction solution including 50 μM luminol and 10 μg/mL horseradish peroxidase (Sigma-Aldrich) and 100 nM flg22 or elf 18 ...
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bioRxiv - Microbiology 2022Quote: ... α-PilC 20 and α-RomY antibodies were used together with goat anti-rabbit immunoglobulin G conjugated with horseradish peroxidase (Sigma) as secondary antibody ...
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bioRxiv - Plant Biology 2022Quote: ... ultra-pure water was replaced with 100 μL reaction solution containing 50 μM luminol and 10 μg/mL horseradish peroxidase (Sigma, USA) together with or without 1 μM flg22 or 0.5μg/μl poly(I:C) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 3 times with 0.05% Tween 20 in PBS and 3 times with PBS before the addition of o-phenylenediamine dihydrochloride peroxidase substrate (Sigma-Aldrich). Reactions were stopped by the addition of 1 M hydrochloric acid ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary substrate was horse radish peroxidase tagged anti-rabbit secondary antibody and anti-goat secondary antibody (1:2500, EMD Millipore). The bands were detected using a chemiluminescent substrate (SuperSignal West Pico ...
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bioRxiv - Molecular Biology 2023Quote: ... The ApopTag Peroxidase In Situ Apoptosis Detection Kit was used for Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (Sigma Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... lysates corresponding to 1 OD600 of cells were analyzed by SDS-PAGE and immunoblotted with either anti-c-Myc (9E10, Covance Research) for Myc-tagged proteins or anti-peroxidase soluble complex antibody (Sigma, P2416) for TAP-tagged proteins ...
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bioRxiv - Microbiology 2023Quote: ... wells were incubated with anti-mouse IgG peroxidase-conjugated (1:1000 in PBS + 1% BSA, Sigma Aldrich, St. Louis, MO, USA) at RT for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation for 1 hour at room temperature with a horseradish peroxidase conjugated anti-mouse secondary antibody (Cat# NA931-1ML, Millipore Sigma) diluted 1:1000 in 3% milk ...
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bioRxiv - Physiology 2023Quote: ... Peroxidase-Glucose oxidase (PGO) enzyme plus color reagent was added to each well (Glucose Assay Kit: Sigma GAG020, PGO Sigma P7119), the plate was incubated at 37°C and the optic density was read at 450 nm using a Tecan Spark Microplate Reader ...
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bioRxiv - Genomics 2023Quote: ... Revelation of the primary antibodies was performed by incubation with goat anti-rabbit Horseradish peroxidase-conjugated secondary antibody (Ref#A0545, Sigma-Aldrick) at 1/10000 ratio in blocking solution for 1 hour at room temperature ...
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bioRxiv - Physiology 2023Quote: ... Peroxidase-Glucose oxidase (PGO) enzyme plus color reagent was added to each well (Glucose Assay Kit: Sigma GAG020, PGO Sigma P7119), the plate was incubated at 37°C and the optic density was read at 450 nm using a Tecan Spark Microplate Reader ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked with 5% BSA at room temperature for 1 hour and incubated with anti-FLAG M2-peroxidase antibody (Sigma-Aldrich) at room temperature for another hour ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was the anti-mouse IgG (whole molecule)–peroxidase antibody produced in rabbit (Sigma; cat# A9044; 1:10000 dilution).
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST) using the ECL System (Merck Millipore; WBKLS0500). Immunoblot analysis was performed using the LI-COR Odyssey Imaging System.
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...