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Citations for Millipore Sigma :
1301 - 1350 of 1366 citations for Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The following day the membrane was washed with T-PBS (0.1% tween) (15 min x 3 times) and appropriate secondary antibody: (i) anti-rabbit antibody conjugated with peroxidase enzyme (Merck Sigma, Burlington, MA) was dissolved in 5% BSA in T-PBS (0.1% tween ...
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bioRxiv - Plant Biology 2024Quote: ... from second leaves of 2-week old wheat plants were incubated with 100 µL of 15 µg/mL Peroxidase from horseradish (Sigma-Aldrich, P6782) and 150 nM luminol L-012 for 16 h in the dark at 15 _ ...
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bioRxiv - Biochemistry 2024Quote: ... The membrane was then washed with TBS-T and incubated with 1:10000 goat anti-rabbit or rabbit anti-mouse poly-HRP secondary antibody (Thermo, 32260) and 1:5000 anti-β-actin-peroxidase antibody (Sigma, A3854) in TBS-T for 1 hr ...
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bioRxiv - Microbiology 2022Quote: ... The control Mbl protein was visualized using primary rabbit anti-Mbl antibodies (dilution 1:10,000) and the secondary goat peroxidase-coupled anti-rabbit IgG antibodies A0545 (Sigma-Aldrich; dilution 1:10,000). Three independent experiments were performed ...
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bioRxiv - Microbiology 2021Quote: ... and PilA2-Flag was detected using a horse radish peroxidase (HRP)-coupled primary anti-Flag antibody at a 1:2000 dilution (Sigma, cat. no. SAB4200119). Enhanced chemiluminescence (ECL ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Biochemistry 2020Quote: ... The anti-C-ter primary antibody and horse radish peroxidase (HRP)-conjugated secondary antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA). TRIzol™ reagent and Complete™ protease inhibitor cocktail were from Roche (Basel ...
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bioRxiv - Neuroscience 2020Quote: ... and reacted with the appropriate horseradish peroxidase (HRP)-conjugated species-specific secondary antibodies (NA934 and NA931, Sigma-Aldrich, MO, USA; AP180P, Millipore, MA, USA) for 1 hr at RT ...
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bioRxiv - Plant Biology 2020Quote: ... 20 μL of (GlcNAc)6 was added in a final concentration of 10 μM to trigger ROS production in the absence or presence of 100 μL of effector protein in a final concentration of 50 μM in measuring solution containing 100 μM L-012 substrate (FUJIFILM, Neuss, Germany) and 40 μg/mL horseradish peroxidase (Sigma-Aldrich, Missouri, USA). Chemiluminescence measurements were taken every minute over 30 min in a CLARIOstar microplate reader (BMG LABTECH ...
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bioRxiv - Microbiology 2021Quote: ... followed by horseradish peroxidase (HRP)-conjugated anti-mouse antibodies (Jackson, 715-035-150, 1:10000) or anti ß-actin-HRP (A3854, Sigma, 1:100000) antibodies ...
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bioRxiv - Microbiology 2020Quote: ... The anti-Psa antibodies (see below) were used at 1:5,000 dilution and the Anti-Rabbit IgG-Peroxidase antibody (Sigma-Aldrich, St Louis, MO) was used at 1:10,000 dilution ...
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bioRxiv - Physiology 2022Quote: ... and with peroxidase- conjugated secondary antibodies (Anti-mouse IgG, HRP conjugate, EMD Millipore, AP160P; Anti-rabbit IgG, HRP-linked, EMD Millipore, 12-348) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... followed by the incubation of cells with horseradish peroxidase (HRP)-coupled anti-FLAG M2 antibody (dilution-1:5000; Sigma-Aldrich; cat. no. A8592), prepared in 1% BSA for 90min ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed with TBST three times for 5 minutes and incubated with a 1:25,000 dilution of horseradish peroxidase-conjugated anti-mouse (Sigma, Cat no. # A9044-2ML6) or anti-rabbit (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St ...
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bioRxiv - Immunology 2022Quote: ... a developing buffer was prepared as follows: one tablet of each 3,3’-Diaminobenzidin peroxidase-substrate (0.7 mg/ml, Sigma-Aldrich, gold and silver tablets) was dissolved in 5 ml distilled water ...
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bioRxiv - Molecular Biology 2019Quote: ... Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit conjugated to peroxidase (Perkin Elmer and Sigma, Aldrich respectively). The latter were developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences).
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bioRxiv - Developmental Biology 2019Quote: Apoptosis induced DNA damage was measured using the ApopTag® Peroxidase In Situ Apoptosis Detection Kit (cat no. S7100, EMD Millipore, Temecula, CA) following the manufacturer’s protocol for paraffin-embedded tissue ...
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bioRxiv - Genomics 2019Quote: ... Approximately 25μg of proteins were analyzed on 10% SDS-PAGE followed by western blots using monoclonal anti-FLAG-M2-Peroxidase (HRP) (Sigma Aldrich, 1:2000) and anti-β actin (1:4000).
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St ...
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bioRxiv - Genetics 2020Quote: ... Protein– antibody interaction was detected with either anti-mouse or anti-rabbit peroxidase-conjugated mouse IgG antibody (Sigma-Aldrich, St Louis, MO, USA), using Amersham™ ECL Plus western blotting detection system (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... Western blots were carried out with two different antibody setups: (i) FLAG tag was incubated with monoclonal ANTI-FLAG® M2-Peroxidase (HRP) produced in mouse (Sigma-Aldrich #A8592) diluted 1:3 000 ...
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bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was then incubated for 1 h at room temperature with sheep anti-mouse horseradish peroxidase (HRP)-conjugated antibody (Catalogue no. GENA931, Millipore Sigma, MO, USA) diluted 1:10000 in Milk/PBST ...
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bioRxiv - Microbiology 2022Quote: ... TCA protein extracts and supernatants were analyzed by immunoblotting with a monoclonal ANTI-FLAG® M2-Peroxidase with the dilution of 1:5000 (Sigma, αFLAG-HRP), rabbit anti-H3 at 1:1000 dilution (D1H2 ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 mM luminol probe 8-amino-5-chloro-7-phenyl-pyrido[3,4-d] pyridazine-1,4(2H,3H) dione (L-012) (Wako Chemicals, Richmond, Virginia, USA) and 20 μg/ml horseradish peroxidase (Sigma, Saint Louis, Missouri, USA). ROS production was measured by a luminol-based assay 74 over 60 min with integration at 750 ms.
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then washed three times with TBST and incubated with peroxidase-conjugated anti-mouse IgG secondary antibody (1:4,000 dilution) (Sigma-Aldrich; catalog no. A4416) at room temperature for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... Washes were followed by the addition of 100 μL of anti-FLAG horseradish peroxidase (HRP)-linked monoclonal antibody (1:10,000 mAb, #A8592, Sigma Aldrich, St. Louis, MO) for 1 h at room temperature with rocking ...
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bioRxiv - Genetics 2023Quote: ... YGTMEPYQTETPRR-amide (Genescript, NJ, USA) as described41 and secondary horseradish peroxidase (HRP) conjugated affinity purified secondary antibody (Cat # AP187P, Millipore-Sigma Aldrich, MO, USA). Briefly ...
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bioRxiv - Immunology 2023Quote: ... The membranes were washed with 1×TBST for three times followed by incubation with a streptavidin-horseradish peroxidase (1:2000, streptavidin-HRP) or an anti-rabbit antibody conjugated with HRP (1:2000, Sigma, Cat#12-348) at room temperature for 1 hr ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and incubated 1 h at RT with peroxidase-conjugated secondary antibodies (anti-mouse, Sigma-Aldrich Cat# A4416, RRID:AB_258167; anti-rabbit, Sigma-Aldrich Cat# A6154, RRID:AB_258284) (1/2000) ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation in either sheep anti-mouse or donkey anti-rabbit IgG-conjugated with horseradish peroxidase (HRP) (Millipore Sigma, GENA931, GENA934, 1:4,000) in TBST at room temperature for two hours ...
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bioRxiv - Physiology 2019Quote: Fragmented DNA was stained by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick-end labeling (TUNEL) – method using the ApopTag® Peroxidase In Situ Apoptosis Detection Kit S7100 (Merck Millipore SAS, Molsheim, France) according to the manufactureŕs instructions (Fig 2 B) ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... The membranes were washed three times with PBS (5 min each time) and were then incubated with secondary peroxidase-conjugated antibody (1:15,000; Sigma-Aldrich, St. Louis, MO, USA). The antibody-bound proteins were detected using Pierce ECL western blotting substrate (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... This was followed by incubation with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgGs or goat anti-mouse IgGs (both Sigma-Aldrich, USA; 1:10000). Protein bands were visualized using SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... diluted anti-YFP antibody or other specific antibodies followed a 1:10,000 (v/v) diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit or a goat anti-mouse antibody (Sigma-Aldrich, St. Louis, MO, USA). The detection signal was developed using the ECL substrate kit as instructed (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and reacted with the appropriate horseradish peroxidase (HRP)-conjugated species-specific secondary antibodies (NA934 and NA931, Sigma-Aldrich, MO, USA; AP180P, Millipore, MA, USA) for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were subsequently incubated with peroxidase-conjugated secondary antibodies (anti-mouse IgG, HRP conjugate, EMD Millipore, AP160P; anti-rabbit IgG, HRP-linked, EMD Millipore, 12-348) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... transferred to a nitrocellulose membrane, and probed with the following antibodies: anti-FLAG M2-HRP (HRP, horseradish peroxidase) conjugate (Sigma, A8592; dilution 1:10,000), rabbit anti-dynein heavy chain DYNC1H1 (Bethyl Laboratories ...
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bioRxiv - Immunology 2022Quote: ... Terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling (TUNEL) assay was carried out using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Millipore Sigma, St. Louis, MO); diaminobenzidine (DAB ...
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bioRxiv - Neuroscience 2020Quote: ... Western blots were treated with antibodies against the appropriate proteins followed by a second reaction with anti-mouse or anti-rabbit IgG conjugated to horseradish peroxidase (Sigma, St. Louis, MO, USA). The SuperSignal West Pico and Femto substrate kits (Pierce ...
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bioRxiv - Microbiology 2020Quote: ... Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody (produced in goat) and Anti-Rabbit IgG (whole molecule)–Peroxidase antibody (produced in goat) were received from Sigma-Aldrich (St. Louis, USA). Rabbit anti-human IgG–HRP was procured from GeNei™ ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody (produced in goat) and Anti-Rabbit IgG (whole molecule)–Peroxidase antibody (produced in goat) was received from Sigma-Aldrich (St. Louis, USA). Rabbit anti-human IgG–HRP was procured from GeNei™ ...
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bioRxiv - Microbiology 2022Quote: ... anti-MeV nucleocapsid (Cat# LS-C144599, LsBio, Seattle, WA, USA) and anti-high affinity (HA) peroxidase (Cat#12013819001, Millipore Sigma, St. Louis, MO, USA) and developed with a KwikQuant western blot detection kit using a KwikQuant Imager (Kindle Bioscience LLC ...
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bioRxiv - Plant Biology 2022Quote: ... ddH2O was replaced by 200 μL of reaction solution containing 100 μM of luminol and 10 μg/mL of horseradish peroxidase (Sigma-Aldrich, Saint-Louis, USA) supplemented with or without 1 μM peptide ...
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bioRxiv - Bioengineering 2024Quote: ... Washes were followed by the addition of 100 μL of anti-FLAG horseradish peroxidase (HRP)-linked monoclonal antibody (mAb, #A8592, Sigma Aldrich, St. Louis, MO) for 1 h at room temperature with rocking ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibodies were detected by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Sigma-Aldrich, A9044 and A5278), followed by incubation with the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: ... YGTMEPYQTETPRR-amide (Genescript, NJ, USA) as described41 and secondary horseradish peroxidase (HRP) conjugated affinity purified secondary antibody (Cat # AP187P, Millipore-Sigma Aldrich, MO, USA). Briefly ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... primary antibody against α-tubulin (T5168) and secondary antibodies (A0545, anti-rabbit; A9044, anti-mouse IgG-peroxidase) were purchased from Sigma-Aldrich (Darmstadt, Germany).
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bioRxiv - Neuroscience 2023Quote: ... horseradish peroxidase (HRP) conjugated secondary anti-rabbit IgG (cat. A0545) and anti-mouse IgG (cat. A9044) were procured from Sigma-Aldrich (St. Louis, MO). Rabbit polyclonal antibody to Iba1 (cat ...