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Citations for Millipore Sigma :
1201 - 1250 of 10000+ citations for 7 Chloro 1 heptene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: LPS- and OGD-treated cells were washed with PBS and stained with 5 μM 2′,7′-dichlorofluorescin diacetate (DCFH-DA; Sigma-Aldrich) for 20 min at 37°C to detect cell reactive oxygen species (ROS) ...
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bioRxiv - Synthetic Biology 2023Quote: ... beginning on day 7 post-partum dams were administered twice-daily a 200ug dose of Bromocriptine (2mg/mL bromocriptine mesylate (Sigma; 1076501) and 2mg/mL tartaric acid (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell suspensions were mixed with 350 µl of freshly prepared lysis buffer (2M NaOH, 7% β-mercaptoethanol) and 350µl of 50% TCA (Sigma, T9159). After spin ...
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bioRxiv - Cancer Biology 2023Quote: ... Myelin and debris removal was performed by a gradient centrifugation of the cell suspension in a 7:3 mix of PBS:Percoll (Sigma-Aldrich, #P4937), with an additional PBS wash ...
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bioRxiv - Microbiology 2024Quote: ... Primary human PBMC were differentiated into macrophages (hMDMs) after culturing for 7 days with RPMI supplemented with 20% heat-inactivated human serum (Sigma-H4522).
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bioRxiv - Synthetic Biology 2024Quote: ... 50 nM readout probes in 10% EC Buffer (1 mL of ethylene carbonate, 7 mL Ultrapure RNase/DNase free water, 2 mL 20x SSC, and 1g Low MW dextran sulfate Sigma #D4911) was added to the cells and incubated for 20 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... containing a sgRNA sequence (5’-TCATCGCTCACAACCAAGTG-3’) targeting exon 7 of ERBB2 (cloned by Twin Helix) and selected with puromycin (Sigma-Aldrich) for 4 days (1 μg/ml puromycin for 72 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 7 x 105 cells/well were plated onto each well of 10 µg/mL Poly-D-lysine (PDL, Sigma-Aldrich, P6407-5MG ...
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bioRxiv - Neuroscience 2024Quote: ... Otr-Cre male mice (aged 5–7 weeks) were anesthetized using a combination of 65 mg/kg ketamine (Daiichi-Sankyo) and 13 mg/kg xylazine (Sigma-Aldrich) via intraperitoneal injection ...
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bioRxiv - Microbiology 2024Quote: ... approximately 7×105 cells growing on 6-well plates were washed with PBS and harvested with Tri Reagent (Sigma Cat#T9424). Total RNA was extracted ...
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bioRxiv - Cell Biology 2024Quote: ... were carefully dissected 7 to 16 days after induction of GAL4 expression at 29°C in Shield and Sang M3 medium (Sigma S3652), keeping the crop ...
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bioRxiv - Cancer Biology 2024Quote: ... Two sets of four EV-rich fractions (7–12 and 16-22) were pooled and concentrated using Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore, USA). Alternatively ...
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bioRxiv - Plant Biology 2024Quote: Flowers at position 6 were gently incubated in staining solution (25 µM (2’,7’-dichlorodihydrofluorescein diacetate) (H2DCF-DA) (Sigma-Aldrich, D6883), 50 mM KCL ...
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bioRxiv - Neuroscience 2024Quote: ... Cre-mediated recombination was induced in mice 3-7 months of age by intraperitoneal injection of 2 mg/day tamoxifen (Sigma-Aldrich) diluted with 300 μl corn oil (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... mice were transferred to a new cage without food at 7:30 am and injected intraperitoneally with vehicle or CNO (Sigma-Aldrich #C08325) at 8:30 am ...
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bioRxiv - Genomics 2021Quote: ... A1413301)-coated 12-well plates at a density in the range of 3-7×105 cells per well in iPS-Brew + 2μM Thiazovivin (Sigma, cat. SML1045-5MG). Alternatively ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared for NMR by combining 16 µL standard with 50 µL serum and placing in a 3 mm NMR tubes (Wilmad®, 7 inch; Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... subsequently lysed and postnuclear supernatants were incubated o/n with monoclonal mouse anti-HA-antibody coupled to agarose beads (clone HA-7, Sigma-Aldich, USA) at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... used for prochloraz quantification was obtained from Rathburn (Walkerburn, Scotland), acetic acid (CAS; 64-19-7, LC-MS grade) from Sigma-Aldrich (Germany), 25:24:1 phenol:chloroform:isoamyl alcohol from Carl Roth GmbH & Co ...
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bioRxiv - Cell Biology 2020Quote: ... using N2B27 2i + Lif medium at 37°C and 7% CO2 in culture flasks coated with 0.1% gelatine (Sigma-Aldrich, #G1890 SIGMA) diluted in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant became the soluble fraction and the pellet was solubilized in urea buffer (30 mM Tris, 7 M Urea, 2 M Thiourea, 4% CHAPS, 1X Protease Inhibitor Cocktail (Millipore Sigma 11873580001), 0.5 mM PMSF ...
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bioRxiv - Biochemistry 2021Quote: ... Female C57BL/6 mice wild type mice (7-9 weeks) were injected intraperitoneally with etomoxir (100 µg, Sigma Aldrich, Cat. No E1905) and/or LPS (1 µg ...
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bioRxiv - Neuroscience 2021Quote: ... during 7 days, in a medium composed of equal volumes of modified C2C12 differentiation medium (DMEM, 2% horse serum (Sigma-Aldrich, #H1270),1% antibiotic-antimycotic (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 12 μg peptides were adjusted to pH 7-8 by adding one volume of 50 mM ammonium bicarbonate (ABC, Sigma-Aldrich, A6141). Offline fractionation by high-pH reversed-phase chromatography was carried out on an Ultimate 3000 HPLC (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Oral microgavage of 6 dpf larval zebrafish was performed using pH indicators m-cresol purple (0.2%; Thermo-Fisher, 2303-01-7) or phenol red (2%; Sigma, 143-74-8). The fish were then imaged on a Leica MZ10F stereoscope using a Leica MC190 HD color camera ...
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bioRxiv - Biochemistry 2021Quote: ... a biotinylated DNA oligonucleotide complementary to the target tRNA (Supplementary Table 7) was immobilized on Novagen Streptavidin Agarose resin (Merck-Millipore, Burlington, MA) in a 0.22 μm Ultrafree-MC filter cup (Merck-Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... the protocol described by Choi et al.65 was applied using home-made wash buffer (50% formamide (Merck Millipore, 75-12-7), 5XSSC (Molecular Biology-P ...
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bioRxiv - Cell Biology 2021Quote: DNA extraction from mouse tail clips obtained at 7-10 days of age was performed using REDExtract-N-Amp™ Tissue PCR Kit (Cat#R4775, Sigma Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: Recombination of the Bbs5flox/flox allele was induced in juvenile CAGG-creERT2 (Jax Mice stock# 004682) positive mice at postnatal day 7 by a single intraperitoneal (IP) injection of 9 mg tamoxifen (Millipore Sigma, T5648) per 40 g body weight ...
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bioRxiv - Immunology 2020Quote: ... Human breast adenocarcinoma cell line (MCF-7) were obtained (ATCC, Manassas, VA, USA) and maintained in DMEM-F12 (Sigma-Aldrich, MO, USA) with 10% fetal bovine serum ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Methyl methanesulphonate (MMS) (#129925) (CAS registry number 66-27-3) and carbendazim (#378674) (CAS no. 10605-21-7) were purchased from Sigma-Aldrich (Merck), UK.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 3 and 7 post-drug or vehicle administration were analyzed to quantify urea levels with a urea assay kit (Sigma-Aldrich, MAK006) according to vendor-provided protocol ...
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bioRxiv - Cell Biology 2022Quote: ... refer to the publication from Susnik et al.32 Similar synthesis protocol was adapted for 59 nm SiO2-RhoB NP using a RhoB isothiocyanate (RBITC) dye (CAS 36877-69-7, Sigma-Aldrich, USA). For 119 nm SiO2-Cy5 NP synthesis ...
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bioRxiv - Immunology 2022Quote: ... BMDMs were generated by culturing marrow cells in poly-L-lysine coated culture plate for 7 days with 50 ng/ml M-CSF in RPMI 1640 (Sigma-Aldrich, R8758) supplemented with 20% FBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% galactose [Sigma-Aldrich #G0625] ...
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bioRxiv - Microbiology 2022Quote: A CRISPR array containing 6 identical spacers targeting the tetR gene flanked by 7 repeats was cloned into pCDF-Duet (Novagen, Merck Millipore) by ligation after NcoI and SalI digestion ...
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bioRxiv - Molecular Biology 2022Quote: ... Stimulation of Jurkat cells for 2-7 hour experiments was achieved with 50ng/ml Phorbol 12-myristate 13-acetate (PMA, Sigma-Alrich, P8139) and 500ng/ml ionomycin calcium salt from Streptomyces conglobatus (ionomycin ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µL of 10 mM of D-Val-Leu-Lys 7-amido-4-methylcoumarin (D-VLK-AMC) (in 10 % DMSO/H2O) (Sigma-Aldrich, #V3138) as plasmin substrate (25 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Protoplasts of Exiguobacterium Strain-Molly (EM-P) were generated by growing the cells in 7%DSS-TSB with Lysozyme and Penicillin G (Sigma Aldrich, Germany). Transformation to protoplast was confirmed by a change in cell morphology (Carl Zeiss ...
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bioRxiv - Plant Biology 2023Quote: ... 600 μl aliquots of diluted chromatin were incubated with 7 μl of the rabbit anti-trimethyl-Histone H3 (Lys27) antibody (Millipore, 07-449) in a rotator at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were fixed for 7 minutes in 2% formaldehyde/0.2% glutaraldehyde solution at room temperature and further incubated with X-Gal (Sigma, Cat. no. B4252) solution for one hour at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... Neural crest intermediates were defined with 7 days of 12 ng/mL FGF2 and 10 μM of the activin–nodal inhibitor SB431542 (Sigma, cat# S4317) in CDM ...
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bioRxiv - Cancer Biology 2023Quote: ... The single-cell suspensions were then seeded in 6-well plates and cultured for 7 to 14 days in the presence of 60 μM 6-thioguanine (Sigma-Aldrich, A4882) [23] ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: Spirodela was grown on half-strength Schenk and Hildebrandt (½ SH, Duchefa Biochemie, #S0225) liquid medium in MagentaTM GA-7 containers (Sigma Aldrich, #V8505), culture dishes (Greiner Bio-One #664160) ...
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bioRxiv - Neuroscience 2024Quote: ... a group of animals was orchidectomized using ketamine (65 mg/kg) and xylazine (7 mg/kg) (Sigma-Aldrich, St. Louis, MO, USA) injected intraperitoneally as anesthetics ...
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bioRxiv - Neuroscience 2024Quote: ... embryos from all genotypes (N = 7 per genotype) were collected at 40 hpf and fixed with 4% paraformaldehyde (PFA; Sigma, Gillingham, UK) in phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS for 30□min at room temperature and subsequently fixed in 4% paraformaldehyde (PFA, Sigma-Aldrich, St. Louis, MO, USA; 15,812-7) in PBS at 4□°C for 1 hour ...
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bioRxiv - Immunology 2022Quote: Bone marrow cells isolated from femur and tibia of C57BL/6J mice were differentiated into BMDMs for 7 – 8 days by a standard procedure (65) in DMEM (Sigma, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2019Quote: ... was followed by monitoring the conversion of the fluorogenic substrate Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (Ac-DEVD-AMC, Sigma-Aldrich, Sintra, Portugal) to 7-amino-4-methylcoumarin (AMC ...
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bioRxiv - Molecular Biology 2019Quote: ... Plates were fixed after up to 7 days of IAA treatment upon initial plating and stained for alkaline phosphatase (AP) activity (Sigma, cat. 86R-1KT) according to the manufacturer’s protocol.