Labshake search
Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for 7 Chloro 1 heptene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and incubated for 30 min with 10 µM 6-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) and 1mg/L Hoechst 33342 dye (Sigma Aldrich, United States) diluted in media ...
-
bioRxiv - Bioengineering 2021Quote: ... the hydrogel was aliquoted into 125 µL aliquots and each aliquot was mixed with 25 μL of thrombin (7 U/mL, Sigma Aldrich, Cat# T6884). Immediately after mixing ...
-
bioRxiv - Biochemistry 2021Quote: ... lysates of African green monkey COS-7 cells (Cell Lines Service, Eppelheim, Germany) and human HEK293T cells (ECACC via Sigma-Aldrich, Poznan, Poland) were analyzed by western blotting ...
-
bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
-
bioRxiv - Microbiology 2021Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at 1:400 dilutions ...
-
bioRxiv - Cell Biology 2021Quote: ... 2.7 mM KCl [Sigma-Aldrich P9541], 19 mM Tris-HCl pH 7.5, and 0.1% v/v Tween 20 [Sigma-Aldrich P7949] in water) for 1 hr at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... was used to determine the peak γTuRC fraction (usually Fraction 7; γ-tubulin, GTU-88 mouse antibody, cat. # T6557, Sigma, St. Louis, MO). A 5 μL sample of the peak fraction was then added to glow discharged copper CF400-CU EM grids (cat ...
-
bioRxiv - Cell Biology 2022Quote: ... DCs were treated on day 7 of culture for 24 h with either 10−6M 1,25 dihydroxy-cholecalciferol (Sigma-Aldrich, St. Louis, MO), 10−6M 1,25 dihydroxy-cholecalciferol and 100 ng/ml LPS (Escherichia coli ...
-
bioRxiv - Microbiology 2020Quote: ... 1,8-Diazabicyclo[5.4.0]undéc-7-ène (DBU), Controlled pore glass (120-200 Mesh, CPG-3000 Å) were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France). Fluorescent rhodamine B silica NPs (Si-NPs ...
-
bioRxiv - Neuroscience 2021Quote: ... The oxidation current signal value was measured initially (i0) and after 300 seconds (i300) of the addition of 5.0 × 10−7 M DA (Sigma, St. Louis, MO, USA). The decrease in the oxidation current signal was used to calculate DA pmols taken up by DAT ...
-
bioRxiv - Cancer Biology 2022Quote: Evaluation of in vitro release of drugs from NIRB-DOX-FA was performed under sink conditions in 50 mg/mL of bovine serum albumin (BSA, heat shock fraction, pH 7, ≥98.0%, Sigma-Aldrich, Oakville, ON, Canada) over five days at 37°C ...
-
bioRxiv - Microbiology 2019Quote: ... pylori strains was assessed by the agar dilution method against a panel of 7 antibiotics purchased from Sigma-Aldrich (St. Louis, MO, USA), including metronidazole (MNZ) ...
-
bioRxiv - Microbiology 2019Quote: ... cells were washed three times with PBS and further cultured at 37°C for 4 days in FBS-free DMEM supplemented with 0.5 μg/mL trypsin (Sigma Aldrich, 9002-07-7) until a clear cytopathic effect was visible ...
-
bioRxiv - Developmental Biology 2019Quote: Fasting plasma samples collected from offspring at PN30 and during the GTT at 6-7 months of age were analysed for insulin levels using a rat insulin radioimmunoassay kit (SRI-13K, Millipore Australia, Kilsyth, VIC). Insulin samples were run in duplicate at 1:5 ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were washed three times with PBS and further cultured at 37°C for 4 days in FBS-free DMEM supplemented with 0.5 μg/mL trypsin (Sigma Aldrich, 9002-07-7) until a clear cytopathic effect was visible ...
-
bioRxiv - Biochemistry 2021Quote: ... Dried samples were suspended in 40 µL of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for 1 hour at 37 °C in a sand bath ...
-
bioRxiv - Biochemistry 2022Quote: ... coated with human plasma fibronectin + 0.1% gelatin in DMEM low glucose + 7% (vol/vol) FBS + P/S + 10 μM cytosine-β-d-arabinoside (AraC, Sigma Aldrich, C1768-500MG). The mitotic inhibitor ...
-
bioRxiv - Plant Biology 2022Quote: ... Corrective flat field images for 405 nm were acquired by using 7-Diethylamino-4-methylcoumarin (Sigma D87759-5G, 50 mg/mL in DMSO). Relative signal was calculated by dividing mean gray values of the R-GECO1 channel by the mean gray values of the mTurquoise channel ...
-
bioRxiv - Physiology 2022Quote: ... Dried samples were suspended in 40 μL of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for one hour at 37 °C in a sand bath ...
-
bioRxiv - Neuroscience 2022Quote: ... recordings were performed with borosilicate glass pipettes (7-15 MΩ) and filled with normal rat Ringer’s solution with 2% biocytin (Sigma, cat # 576-19-2). The pipette was positioned with a micromanipulator (Luigs & Neumann ...
-
bioRxiv - Neuroscience 2023Quote: The following commercially available antibodies were used for the immunostaining analyses: mouse anti-HA (clone HA-7, Sigma-Aldrich, St. Louis, MO, USA), mouse anti-PSD95 (clone K28/43 ...
-
bioRxiv - Microbiology 2023Quote: ... overnight cultures of bacteria in MMA were diluted to an OD600nm of 0.0055 in fresh medium adjusted to pH 7 or 5.5 with 10 mM MES buffer (Sigma-Aldrich, Saint Louis, MO, USA). The different sensitivity assays were then incubated at 37°C ...
-
bioRxiv - Biophysics 2023Quote: Photodynamic effect of MB-loaded nanoMOFs and other formulations was measured using 2’,7’-dichlorofluorescin diacetate (H2DCF-DA) (Sigma-Aldrich, St Louis, MO), which converts upon ROS generation into fluorescent oxidation product ...
-
bioRxiv - Genetics 2024Quote: ... bulk embryos were isolated by vigorous mixing in hypochlorite solution (40 ml dH2O, 7 ml sodium hypochlorite Sigma #425044, 3 ml 1M KOH) for 6-7 minutes ...
-
bioRxiv - Molecular Biology 2024Quote: ... for MCF-7/MDA-MB-231/ARPE-19/HEPG2/MCF10A cells and 10% (v/v) bovine calf serum (Sigma-Aldrich, Cat. No. 12133C) for NIH3T3 cells ...
-
bioRxiv - Microbiology 2024Quote: ... 15 µg of total protein in a volume of 47 µl 1x sample buffer was treated with 7 U of benzonase (#70746; Merck Millipore, Burlington, MA, USA) in dilution buffer (20 mM HEPES pH 8.0 ...
-
bioRxiv - Neuroscience 2024Quote: ... Washed pellets were resuspended in a 7:3 ratio of chemotaxis buffer (see above) and Optiprep (60% iodixanol in sterile ddH2O w/v, Sigma-Aldrich, D-1556), resulting in a net dilution to 18% iodixanol w/v ...
-
bioRxiv - Cancer Biology 2019Quote: ... The Fab and Fc were buffer exchanged with PBS and concentrated using Amicon Ultra centrifugal filter (7 kDa mwco, Millipore Sigma, St. Louis, MO).
-
bioRxiv - Neuroscience 2021Quote: ... CAS # 7646-85-7 and ZnC4H6O4; CAS #557-34-6) and arsenic (NaAsO2; CAS #7784-46-5) (all from Sigma-Aldrich, St Louis, MO). The metallic compounds were either dissolved in 30% (w/v ...
-
bioRxiv - Microbiology 2019Quote: ... C57BL/6 and Trem-2 KO males with 7-8 weeks of age received PBS or 20%v/v carbon tetrachloride (CCl4, Sigma, St. Louis, MO, USA) in olive oil ...
-
bioRxiv - Cancer Biology 2020Quote: ... High-titer virus was collected at 36 and 60 hours following transfection and used to infect cells in presence of 7 μg/ml polybrene (Sigma-Aldrich, Cat. H9268-5G) for 12 hours ...
-
bioRxiv - Immunology 2022Quote: ... the cells were transferred to a single well containing a monolayer of mitomycin C (Cat no. 50-07-7, Millipore Sigma, Merck, Darmstadt, Germany)-treated OP9/ OP9-DL1 cells or were kept unaltered ...
-
bioRxiv - Neuroscience 2020Quote: For calcium imaging experiments the highest purity available was chosen for all chemicals used in this study: L-Alanine (Sigma-Aldrich, 56-41-7), L-Glutamine (Sigma-Aldrich ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 6–7 weeks post-infection) were obtained from infected female mice by hepatic portal vein perfusion with 37°C DMEM (Sigma-Aldrich, St. Louis, MO) plus 10% Serum (either Fetal Calf Serum or Horse Serum ...
-
bioRxiv - Neuroscience 2020Quote: ... olfactory bulbs of postnatal (PN) day 0-7 mice were collected and placed in an enzyme mix: 300μg/ml hyaluronidase (Sigma, Cat# H3631, St. Louis, MO), 30U/ml dispase I (Sigma ...
-
TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... and, 7 days after germination, were transferred to plates with fresh ½ MS medium containing 10 µM NPA (Naphthylphthalamic acid, (Sigma-Aldrich, St. Louis, MO) for 24h before shift to 10 µM IAA.
-
bioRxiv - Plant Biology 2022Quote: ... Membranes were washed with TBST buffer (three times, 7 minutes each) and incubated with anti-mouse-HRP or anti-rabbit-HRP (Sigma-Aldrich; 20,000 times dilution) for 1 hour ...
-
bioRxiv - Microbiology 2023Quote: ... The rest of the pre-cleared lysates was then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at a 1:400 dilution ...
-
bioRxiv - Microbiology 2023Quote: ... All well plates and culture flasks were coated prior to seeding with 7 µg/mL rat tail tendon collagen I (Sigma-Aldrich, St. Louis, MO) and rinsed with PBS 3 times ...
-
bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...
-
bioRxiv - Zoology 2023Quote: ... was coated with 100 μl of either 10 or 100 mM caffeine (CAS: 58-08-2, Biopack, Buenos Aires, AR) or quinine (CAS: 6119-47-7, Sigma-Aldrich, St Louis, US) dissolved in distilled water ...
-
bioRxiv - Neuroscience 2023Quote: ... and 3 μg/mL + 7 μg/mL FRD+U (5-Fluoro-2′-deoxyuridine, Sigma-Aldrich, Cat# F0503 and Uridine, Sigma-Aldrich, Cat# U3003-5G). 120 µL of cell suspension was carefully plated onto each coverslip and allowed to adhere for 2 hours at 37°C and 5% CO2 ...
-
bioRxiv - Neuroscience 2023Quote: ... 35)The CGCs were prepared from postnatal ICR mice aged 5-7 days by tissue dissociation utilizing 0.05% trypsin (Sigma Aldrich, St. Louis, MO, USA) and DNase I (Roche Applied Science ...
-
bioRxiv - Biochemistry 2020Quote: ... Iowa City, IA), dynein heavy chain (P1H4, gift from T. Hays, University of Minnesota, Minneapolis, MN) and anti-HA antibody (Sigma, H 6533 (clone HA-7).
-
bioRxiv - Cell Biology 2020Quote: ... 3-Isobutyl-1-methylxanthine (IBMX, ≥ 99%), N-acetyl-L-cysteine (NAC, ≥ 99%) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA, ≥ 97%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pregnant mare serum gonadotropin (PMSG ...
-
bioRxiv - Neuroscience 2020Quote: (+)-MK-801 hydrogen maleate (CAS Number: 77086-22-7) and D-amphetamine hemisulfate salt (CAS Number: 51-63-8) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All drugs were dissolved in water at the same conditions as the home tanks ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... ground and homogenized western corn rootworm larval tissue (3-7 larvae per biological replicate, n=8-11) using the GenElute Universal Total RNA Purification Kit (Sigma-Aldrich, St. Louis, MO, USA). A NanoDrop spectrophotometer (ND-1000 ...
-
bioRxiv - Cell Biology 2021Quote: ... pH 7) gradient (20%, 40%, 60%, 80%, 100%), incubated 3 times (1h, o/n, 2h) in 100% dichloromethane (DCM) (Sigma-Aldrich, St. Louis, MO, USA) to remove hydrophobic lipids ...
-
bioRxiv - Plant Biology 2021Quote: ... leaf epidermal strips were peeled from tobacco leaves and floated on a solution of 50 μM 2’-7’dichlorofluorescein diacetate (DCFH-DA; Sigma Chemicals, St Louis, MO, USA). The leaf stripe samples were collected after salt stress treatment for the indicated time ...
-
bioRxiv - Microbiology 2020Quote: ... 48 and 72 hours) of exposure to simulated solar radiation was performed by using 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) (Sigma Aldrich-Merck KGaA, Darmstadtm Germany) solubilized in ethanol ...