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Citations for Millipore Sigma :
1001 - 1050 of 10000+ citations for 7 Chloro 1 heptene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... 2.5-month-old CON male and female (C57Bl/6J, n=7-9/sex/group) were injected with 0.5 mg/kg of raloxifene hydrochloride (SIGMA) for 6 weeks ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then labeled with the MDCC (7-Diethylamino-3-[N-(2-maleimidoethyl) carbamoyl]coumarin) from Sigma-Aldrich. Unreacted MDCC was removed by ion exchange using a Bio-Rex70 column ...
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bioRxiv - Cancer Biology 2021Quote: ... the eggs were inoculated with 1.5 x 106 MCF-7 cells resuspended in 50 ul of Matrigel (Sigma Aldrich) on the blood vessel of the CAM ...
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bioRxiv - Cancer Biology 2020Quote: ... under hypoxic conditions (7% O2) at 37°C and in mammosphere medium containing 10 nM HEPES (Sigma-Aldrich, Germany), 10 µg/ml insulin (all from PAN-Biotech ...
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bioRxiv - Cell Biology 2021Quote: Cellular ROS was measured by incubating cells with 100 µM 2′,7′-Dichlorofluorescin diacetate (DCFH-DA) (D6883, Sigma-Aldrich) in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Biochemistry 2021Quote: ... The pellet was resuspended in 7 ml of PBS in the presence of protease inhibitors (Sigma-Aldrich, Steinheim, Germany) and then sonicated for 30 min of 30 cycles (30 s run and 30 s rest ...
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bioRxiv - Cell Biology 2021Quote: Cells were FACS-sorted and cultured in AM for 7 days with 10μM of Wzb-117 or with 100 nM Rapamycin (Sigma). For glucose deprivation experiment ...
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bioRxiv - Plant Biology 2021Quote: The bacterial strains used were pre-grown for 7 d in Tryptic Soy Broth 50 % (TSB 50 %, Sigma-Aldrich), and washed with 10 mM MgCl2 (series of centrifugations and removal of supernatant ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL of bead solution was removed and replaced with 200 μL of phenol:chloroform:isoamyl alcohol (Sigma; pH 7-8) before the stool was added ...
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bioRxiv - Plant Biology 2021Quote: ... Fractions were desalted on C18 Cartridges (Empore™ SPE Cartridges C18, bed I.D. 7 mm, volume 3 ml, Sigma), and reconstituted in 0.1% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... The reagents 7-ethoxycoumarin (CAS: 31005-02-4, purity: 99.7 %) and RNAlater®was purchased from Sigma-Aldrich (Germany), TRIzolTM reagent from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: Intracellular ROS levels were measured by monitoring the oxidation of cell permeable 2′,7′-dichlorofluorescein diacetate (DCFHDA, Sigma-Aldrich) to fluoros-pectrophotomete at excitation and emission wavelengths of 480 and 535 nm ...
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bioRxiv - Cell Biology 2021Quote: ... colonies were grown for 7 to 10 days after which they were fixed and stained using Coomassie blue (50% methanol, 7% acetic acid and 0.1% coomassie blue (all Sigma)) ...
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bioRxiv - Bioengineering 2022Quote: MCF-7 cells were cultured in DMEM growth media supplemented with 10mg/L insulin (Sigma Aldrich, St. Louis, MO), 1% v/v Penicillin-Streptomycin mix (Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... buffer (7.7 mM NaN3, 0.1% bovine serum albumin [BioWest], 0.2% Triton X-100 and 0.05% Tween-20 [Sigma] in PBS) supplemented with 10% normal goat serum (NGS ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... C5b-7 labelled bacteria at OD600 = 0.05 (5×107/ml) were incubated with 10 µg/ml bovine trypsin (Sigma) for 20 minutes at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... The blocked membrane was incubated with the primary antibodies: mouse anti-HA (2,000×, Sigma-Aldrich HA-7, Massachusetts, USA), mouse anti-α-actin (20,000× ...
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bioRxiv - Microbiology 2022Quote: ... and resuspended at an OD600 of 0.4 (∼1× 108 colony forming units (CFU)/mL) in phosphate-buffered saline (PBS; pH 7) with 0.1% bovine serum albumin (BSA; Sigma). Bacteria (1.25 × 106 CFU ...
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bioRxiv - Cancer Biology 2022Quote: ... snap-frozen tumor tissue samples were placed in 5-7 ml of PBS with 0.25% sodium deoxycholate (Sigma-Aldrich), 1% octyl-β-D glucopyranoside (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2019Quote: ... column equipped with an Ascentis Express C18 150 × 2.1 mm ID (2.7 μm) column equipped with an Ascentis Express C18 5 × 2.1 mm ID (2.7 µm) guard column (Sigma-Aldrich). The mobile phase consisted of water and methanol (both added 0.1 % formic acid) ...
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bioRxiv - Cell Biology 2019Quote: ... S2 cells were settled for 20’ on polylysine coated cells and fixed for 7’ with 3.7% formaldehyde solution (Sigma) in PBS or in −20°C methanol ...
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bioRxiv - Neuroscience 2021Quote: ... adult flies upon eclosion were kept for 7-8 days before the experiment on food containing 0.5 mM all-trans retinal (Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... Each piece of sclera was rinsed with PBS and then placed into 20 mL of pH 7.5-8.0 extraction medium containing: >1000 BAEE unit/mL trypsin (9002-07-7, Sigma-Aldrich), 4 mM ethylenediaminetraacetic acid (60-00-4 ...
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bioRxiv - Bioengineering 2019Quote: ... or 48-well plates (with or without 7×7mm glass coverslips) pretreated with 50 µg/mL human fibronectin (Millipore).
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bioRxiv - Immunology 2019Quote: ... Cells were incubated for 7 minutes on ice in Red Blood Cell Lysing Buffer Hybri-Max™ (Sigma-Aldrich) for the removal of contaminating erythrocytes ...
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bioRxiv - Cell Biology 2019Quote: ... chopped thoroughly and resuspended in 10 ml digestion solution containing 7 ml Hanks’ Balanced Salt Solution (HBSS, H9269, Sigma), 0.23 g bovine serum albumin (BSA ...
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bioRxiv - Genetics 2020Quote: ... and ~7 μl of each dilution was spotted on SD-Ura or SD plates containing 5-FOA (Sigma Aldrich). Plates were incubated at indicated temperatures for 2-4 days.
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bioRxiv - Microbiology 2021Quote: ... and incubated with anti-HA antibody (3F10; Roche Diagnostics, Mannheim, Germany or HA-7; Sigma, Saint Louis, MI, USA), anti-DDX6 (A300-460A ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stimulated with 50 μL of 10−7 M phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) or N-formyl-methionyl-leucyl-phenylalanine (fMLP ...
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bioRxiv - Cell Biology 2021Quote: ... Fifteen µg of cell lysate from each hESC line was incubated in 150 µl acetate buffer (pH 4.0) containing a final concentration of 62.5 µM Ala-Ala-Phe-7-amido-4-methylcoumarin (AAF-AMC) (Sigma #A3401) for 20 h at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: TPP1 enzyme activity was measured fluorometrically based on fluorescence of the chromophore 7-amino-4-methylcoumarin (AMC) (Sigma #A9891) as described previously (Lojewski et al. ...
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bioRxiv - Cell Biology 2021Quote: 7-8 week-old age and sex-matched mice were used for treatments with arsenic (5ug/g, Sigma, #202673), pI:C (20 ug/g ...
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bioRxiv - Biochemistry 2022Quote: Three-day-old germinated seedlings were sprayed with a 0.5% pH 7 solution of phosphite (Sigma, St Louis, USA) using a hand-triggered spray bottle and untreated seedlings were sprayed with water 31–33 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transferred to a 5-7 µl drop of CZB covered with mineral oil (Sigma Aldrich, Cat# M5310). A glass cover slip was placed on top of the media drop and pressed down on the edges to spread the media to cover the entire surface of the cover slip ...
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bioRxiv - Molecular Biology 2022Quote: ... Three-mL liquid cultures were inoculated with a single colony and supplemented with 2′,7′-dichlorodihydrofluorescein diacetate (DCFHDA; Sigma) with a final concentration of 1 μM ...
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bioRxiv - Neuroscience 2023Quote: ... 1.25 mM NaH2PO4, 2.5 mM KCl, 0.5 mM CaCl2, 7 mM MgCl2, 25 mM glucose; all from Sigma-Aldrich) saturated with carbogen ...
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bioRxiv - Neuroscience 2023Quote: ... or high pH medium supplemented with 0,01 μM 7-alpha,25-dihydroxycholesterol (7α,25OHC) (Sigma Aldrich, 64907-22-8) was added to the bottom of the chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... The cortex was then dissected and dissociated using a papain solution (0.5mM of EDTA, 1mM L-Cysteine and 0.4mg/mL of papain (7 units of papain/mL) (Sigma, #76216)) at 37°C in a water bath for 25 min ...
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bioRxiv - Cell Biology 2024Quote: COS-7 (ATTC) cells were grown on #1.5 glass coverslips in DMEM containing 10% fetal bovine serum (Sigma-Aldrich), washed twice with 2 ml of 100 mM sodium phosphate and fixed for 2 h in 2 ml PLP (2% [wt/vol] paraformaldehyde in 87.5 mM lysine ...
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bioRxiv - Bioengineering 2023Quote: ... Pooled fractions (7-10) and (11-30) were then concentrated using 10 kDa cutoff filters (Amicon Ultra-15, Millipore). Each fraction was subjected to protein concentration measurement ...
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bioRxiv - Microbiology 2023Quote: ... promastigotes were incubated for 7 days to maximize infective metacyclics (i.e., those isolated through a step Percoll gradient; Sigma). Amastigotes typically were grown axenically for 5 days prior to use [17] ...
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bioRxiv - Molecular Biology 2023Quote: ... At 7 weeks of age female animals were injected IP with 200mg/kg of tamoxifen (Millipore Sigma, Burlington, MA) dissolved in corn oil for two consecutive days.
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bioRxiv - Immunology 2023Quote: ... Mice were sacrificed at day 7 (X31) and day 4 (RSV) post infection (p.i.) and lung lobes were stored in RNA-later (Sigma). Lung tissue was homogenized using a TissueLyser LT (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ROS production in BMDMs was measured using the membrane-permeable fluorescent probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFHDA) (Sigma, #D6883). BMDMs were plated in 96-well plates at 1×105 cells/well ...
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bioRxiv - Microbiology 2023Quote: ... indole and 7-cyanoindole solutions were prepared at 5 mg/ml in vehicle (10% DMSO and Millipore-filtered water), aliquoted and stored at −20 °C ...
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bioRxiv - Biophysics 2023Quote: ... Glyceryl trioleate was either >99% purity (T-235, NuChek Prep) or 97% purity (CAS: 122-32-7, Sigma Aldrich). When dissolving PL within the TAG prior to creating the caps ...
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bioRxiv - Genetics 2023Quote: ... 30 hours post-fertilization old embryos were exposed to 20 μM 2′,7′-dichlorofluorescein diacetate (DCFH-DA, Sigma-Aldrich) for 1 hour at 28.5 °C in the dark followed by washing with embryo water a minimum of three times 31 ...
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bioRxiv - Immunology 2023Quote: ... At 5-7 days post infection mice were orally gavaged with 200 µl 2mg/ml loperamide hydrochloride (Sigma Aldrich) to minimize peristaltic movement for imaging ...
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bioRxiv - Cancer Biology 2023Quote: RNA fractionation of MDA-T32 and MCF-7 cells was performed using the Nuclei EZ lysis buffer (Sigma-Aldrich) as previously described.48 RNA was isolated from the nuclear and cytoplasmic cell fractions using RNA Extracol and GeneMATRIX Universal RNA Purification Kit (EURx) ...
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bioRxiv - Biochemistry 2023Quote: ... Muse® cell analyzer kits for measurement of caspase3/7 (MCH100108) and cell cycle (MCH100106) were from EMD Millipore. Unless otherwise noted ...