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Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for 5 Chloro 1 vinyl 2 pyridone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... 1% phosphatase inhibitor cocktails #2 (Sigma P5726-1ML) and #3 (Sigma P0044-1ML) ...
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bioRxiv - Genetics 2024Quote: ... 1:2000 (04-902 DW-2, Sigma-Aldrich) Goat anti-rabbit HRP and Goat anti-mouse HRP secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... and Dox (2 µg ml−1, from Sigma). Culture medium was replenished every other day during reprogramming to maintain the activity of the supplements ...
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bioRxiv - Biophysics 2024Quote: ... 1% phosphatase inhibitor cocktails #2 (Sigma P5726-1ML)/#3 (Sigma P0044-1ML) ...
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bioRxiv - Cancer Biology 2024Quote: ... and phosphatase inhibitor cocktails 1-2 (Sigma Aldrich). Protein concentration was measured by BCA protein assay (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... 6-diamino-2-phenylindole (DAPI, 1:1000, Sigma) as well as secondary antibodies (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... and doxycycline hyclate (2 µg.mL–1, Sigma, D9891). From day 4 to day 5 ...
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bioRxiv - Immunology 2024Quote: ... solution with 1% 2-β-mercaptoethanol (Sigma-Aldrich). To enrich for Lgr5+-ISC population ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 mM 2-mercaptoethanol (Sigma, Cat #M6250). Samples were run on a 4-20% MINI-PROTEAN TGX Stain-Free gels (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... and 2 mg L-1 glycine) (Sigma-Aldrich). Plant growth regulators ...
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bioRxiv - Neuroscience 2024Quote: ... 1% phosphatase inhibitor cocktail 2 (Sigma Aldrich, #P5726); and 1% phosphatase inhibitor cocktail 3 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... and dispase II (2 U·mL−1; Sigma-Aldrich), filtered through a 100-µm cell strainer ...
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bioRxiv - Developmental Biology 2024Quote: ... 50× 1-phenyl-2-thiourea (PTU; Sigma-Aldrich) was added to 0.3× Danieau Buffer at a final concentration of 1 × PTU to inhibit the formation of pigmen ...
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bioRxiv - Developmental Biology 2024Quote: ... rat anti-Laminin-2 (1:500; Sigma; L0663), and rat anti-Ki67 (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... 2 IU ml−1 heparin (Sigma-Aldrich, H3149), 5% human solvent detergent pooled plasma AB (Gemini ...
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bioRxiv - Developmental Biology 2024Quote: ... 1× phosphatase inhibitor cocktail 2 (Sigma, P5726-5ML), pH 8.2] on ice for 10 min.
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bioRxiv - Cell Biology 2024Quote: ... Phosphatase inhibitors (Cocktail 2, 1:100, Sigma-Aldrich) and protease inhibitors (P8340 ...
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bioRxiv - Immunology 2024Quote: ... the allosteric FFA2R modulator Cmp58 ((S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide) and adenosine 5′-thriphosphate disodium salt hydrate (ATP) were from Sigma-Aldrich (Merck, Burlington, MA, USA). The FFA2R antagonist CATPB ((S)-3-(2-(3-Chlorophenyl)acetamido)-4-(4-(trifluoromethyl ...
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bioRxiv - Biochemistry 2021Quote: ... 1:5 or 1:10 (v/v) ratio with sinapinic acid matrix (Sigma; 10 mg/mL in water/acetonitrile/trifluoroacetic acid ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mL of freshly prepared 5 mg mL−1 Clostridium histolyticum collagenase (Sigma) in Tris calcium buffer with ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor (Roche ...
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bioRxiv - Synthetic Biology 2022Quote: ... 100 μL 1×PBS 1% saponin (Sigma-Aldrich, Cat # 8047-15-2) were added ...
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bioRxiv - Cell Biology 2021Quote: ... CCCP 2 µM and Antimycin A 1 µM + 1 µM Rotenone (Sigma). Measurements were taken over 2-min intervals ...
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bioRxiv - Microbiology 2020Quote: ... 2 and 3 emulsified 1:1 in Freund’s incomplete adjuvant (Sigma-Aldrich) on days 0 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal against alpha-tubulin (1:1000; b5-1-2, Sigma, T6074). Actin was stained using 100 nM of phalloidin-iFluor488 for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... is added 1:1 together with 2 µg/mL doxycycline (Sigma-Aldrich) to induce TetO gene expression ...
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bioRxiv - Microbiology 2024Quote: ... + 2% HI FBS + 1% Anti-anti + 1% (w/v) carboxymethylcellulose (Sigma-Aldrich)) was added to the plate (180 µL/well) ...
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bioRxiv - Microbiology 2024Quote: ... + 2% HI FBS + 1% Anti-anti + 1% (w/v) carboxymethylcellulose (Sigma-Aldrich)) was added to cells ...
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bioRxiv - Plant Biology 2022Quote: ... 20 mM Tris–HCl [pH 8.0], 5 mM imidazole, 5% glycerol, 1 mM PMSF, 1/10 protein inhibitor cocktail: Sigma, St. Louis, MO, USA). After centrifugation for 40 min at 21,000 xg ...
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bioRxiv - Neuroscience 2022Quote: ... 2 DIV differentiated cells were treated with 1 µM CDK1/2 inhibitor III (Millipore, 217714) in control medium supplemented with 2% FBS for 24 h ...
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bioRxiv - Neuroscience 2022Quote: ... and stained with microtubule-associated protein 2 (MAP 2) antibody (1:500, Sigma aldrich M9942), ALK (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were resuspended in pre-chilled 1mL of 2:2:1 acetonitrile (34851, Millipore Sigma) + methanol (439193 ...
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bioRxiv - Genomics 2023Quote: ... undifferentiated iPSC colonies were treated with 5 μM of the Y27632 compound and dissociated to single cells with Accutase (Millipore, 1:2 dilution in PBS 1X). Four million dissociated cells were seeded in each well of a 6-well plate and cultured in mTeRS1 with 10 μM Y27632 compound on an orbital shaker at a speed of 95 rpm ...
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bioRxiv - Biochemistry 2022Quote: ... they were cultivated 72 hours in the presence of 2 μM DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol (PPMP) (Sigma-Aldrich) to inhibit the synthesis of glucosylceramide-based GSLs 75 and incubated with fluorescent SaroL-1 as previously described ...
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bioRxiv - Neuroscience 2022Quote: ... For the blockade of cholinergic receptors in CA1 200nl of atropine (2 mM) and mecamylamine (2 mM) (1:1) (Sigma Aldrich) were microinjected at 1.2mm DV (IM-9B microinjector ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with anti-Sulf-1/2 primary antibody (anti-Sulf-1: rabbit polyclonal, Sigma, SAB1410410, anti-Sulf-2: rabbit monoclonal clone 2B4, Sigma, MABC584) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 50 hpf zebrafish embryos using the lysis buffer from the calpain 1/2 activity kit (InnoZyme™ Calpain 1/2 Activity Assay Kit CBA054 purchased from Merck Millipore). Protein concentration was adjusted to 2 mg/mL and calpain enzymatic activity was measured in microtubes following the manufacturer instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 100 μL of 2 mg/mL IG[pS]TENLK was mixed 1:1 with 250 mM Ba(OH)2 (Sigma-Aldrich) in a total volume of 200 μL and incubated at 37°C for 90 min ...
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bioRxiv - Bioengineering 2024Quote: The alkaline comet assay was performed by mixing 1.5 × 105 cells 1:1 with 2 % low melting point agarose (2-Hydroxyethylagarose, Type VII, Sigma-Aldrich, A4018) at 37°C ...
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bioRxiv - Physiology 2022Quote: ... Membranes were blocked for 1 hour at room temperature in 5% milk or 5% BSA (Millipore Sigma) in 1X Tris-buffered saline + 0.1% Tween 20 (TBST) ...
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bioRxiv - Microbiology 2021Quote: ... were plated on synthetic complete (SC) plates containing 1□mg/ml 5-FOA (5-fluorotic acid; Sigma) and on non-selective SC plates/ ...
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bioRxiv - Biochemistry 2022Quote: ... The [5′-32P] cytidine 3′,5′-bisphosphate was prepared by incubating 1 mM cytidine 3′-monophosphate (Sigma) with 312.5 pmole [γ-32P] ATP (6000 Ci/mmol ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% Glycerol) supplemented with 1 mM Phenylmethylsulfonyl fluoride (Carl Roth) and 5 mM β-Mercaptoethanol (Sigma Aldrich). The resuspended cells were disrupted by sonication with a Branson Digital Sonifier (Model 102C ...
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bioRxiv - Cancer Biology 2023Quote: ... The 5 µl samples were injected onto a ZIC-pHILIC column (150 mm × 2.1 mm; i.d. 5 µm) with a guard column (20 mm × 2.1 mm; i.d. 5 µm) (Millipore) for LC separation ...
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bioRxiv - Neuroscience 2024Quote: ... were injected onto a ZIC-pHILIC column (150 mm × 2.1 mm; i.d. 5 µm) with a guard column (20 mm × 2.1 mm; i.d. 5 µm) (Millipore) for LC separation ...
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bioRxiv - Cancer Biology 2020Quote: ... and Hs 766T were plated at a density of 2×104 cells/cm2 and were treated for six days with 100 μM 5-aza-2’deoxycytidine (Sigma Aldrich, St. Louis, MO) or for 24 hours with 50 nM Trichostatin A (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... strains were streaked from cryostocks on LB agar (10 g L-1 tryptone, CRITERION™, 5 g L-1 yeast extract, Sigma-Aldrich and 5 g L-1 NaCl) and incubated for 2 days at 30 °C ...
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bioRxiv - Microbiology 2021Quote: ... aeruginosa strains were routinely refreshed from frozen stocks at -80°C and maintained on YPD (1% Bacto yeast extract, 2% Bacto peptone, 2% dextrose, 2% Bacto agar) plates or Pseudomonas isolation agar (Sigma-Aldrich) for in vitro experiments and LB agar (10 g/liter Bacto tryptone ...
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bioRxiv - Molecular Biology 2024Quote: ... Three different replicas of the irradiated cell lines and the negative control were incubated at 37°C in a 5% CO2 atmosphere for 48 h with the thymine analog 5-bromo-2’-deoxyuridine (BrdU, Sigma-Aldrich, St. Louis, MI, USA). Colcemid was added 24 h before the extraction ...
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bioRxiv - Cancer Biology 2021Quote: ... a sterile β-mercaptoethanol solution was prepared (5 μl of 2-mercaptoethanol (Sigma-Aldrich, Munich, Germany)) in 8.45 ml of PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Brdu-ChIP was performed after addition of 20 μM BrdU (5-bromo-2′-deoxyuridine, Sigma Aldrich) directly to HeLa culture medium for 1 h ...