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Citations for Millipore Sigma :
1051 - 1100 of 10000+ citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... nuclei and actin were stained in PBST containing 5 µg/ml 4′,6-diamidino-2– phenylindole dihydrochloride (DAPI; D9542, Sigma-Aldrich) and Alexa Fluor 488 phalloidin (diluted in 1/100 ...
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bioRxiv - Cell Biology 2024Quote: The femurs of 10-week-old mice were analyzed by VivaCT 2 weeks before (baseline) and 4 weeks after a single intraperitoneal injection (i.p.) of 5 mg/kg doxorubicin (Sigma-Aldrich, MO) formulated in H2O at 1 mg/ml or vehicle ...
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bioRxiv - Cell Biology 2024Quote: ... fibroblasts were fixed in 4% formaldehyde in PBS for 5 min at 37°C followed by cold 2% formaldehyde in 6.25% methanol (Sigma-Aldrich, #32213) in PBS for 1 min at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... followed by 1 hour incubation with 2 mM methyl-β-cyclodextrin (MβCD) (Sigma-Aldrich/Merck, #C4555).
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bioRxiv - Biochemistry 2022Quote: ... The cysteine bonds were reduced with a final concentration of 5 mM Tris (2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma, 646547) for 60 min at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Slides were incubated in 4’,6-diamidino-2-phenylindole (DAPI) to label nuclei (1:10,000 dilution, Sigma). TUNEL assay was conducted with ApopTag Fluorescin Direct In Situ Apoptosis Detection Kit (Millipore ...
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bioRxiv - Developmental Biology 2020Quote: ... larval sections were counterstained with DAPI (4′,6diamidino-2-phenylindole, Sigma-Aldrich; 1:1000 μL PBS dilution) and incubated for 15 min in the dark at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Cell nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI, 1 µg/ml, Sigma-Aldrich). Sections were coverslipped using Vectashield Antifade Mounting Medium (Vector Labs ...
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bioRxiv - Biochemistry 2020Quote: ... for 20 min and optionally counterstained with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole, Sigma) nucleic acid stain ...
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bioRxiv - Neuroscience 2020Quote: ... and counter-stained using 4’,6-diamidino-2-phenylindole (DAPI) for nuclei (Sigma, D9542-10MG, 1:1000). Confocal images of the cortex of non-transgenic littermates and arcAβ mice were obtained using a Leica SP8 confocal microscope (Leica Microsystems GmbH ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were inserted into a 1 μg/ml solution of 4’,6-diamidino-2-phenylindole (DAPI, Sigma) for 10s and ...
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bioRxiv - Plant Biology 2022Quote: ... they were supplemented once per week with 1/4 strength Hoagland No.2 media (Sigma Aldrich H2395). For tissue harvesting ...
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bioRxiv - Cell Biology 2021Quote: ... Cell nuclei were stained with DAPI (1:1000, 4’ s-6-diamidino-2-phenylindole, Sigma Aldrich, USA) for 10 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... was previously isolated on the International Streptomyces Project 2 medium (ISP2, dextrose 4 g.L-1 [Sigma-Aldrich, St ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µL/g) of the psychedelic drug 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) (Sigma Aldrich) was delivered and HTR recorded and quantified ...
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bioRxiv - Cancer Biology 2023Quote: ... For nuclear staining 1 μg/ml of DAPI (4′,6′-diamidino-2-phenylindole) (Sigma Aldrich, Taufkirchen, Germany) was used ...
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bioRxiv - Cell Biology 2023Quote: ... stained for 10 min with 4′,6-diamidino-2-phenylindole (DAPI; Sigma, D9542, 1:1000 in PBS), and washed extensively in PBS before mounting in RapiClear® 1.52 (SUNJin Lab ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell nuclei were visualized using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, 1 µg/ml).
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bioRxiv - Cancer Biology 2023Quote: ... Cell nuclei were visualized using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, 1 µg/ml). Fluorescence imaging was performed on a Nikon Eclipse Ti-E epifluorescence microscope ...
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bioRxiv - Neuroscience 2023Quote: ... along with DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride, Millipore-Sigma # 10236276001, used at 1 μg/mL), washed twice with PBSX for 15 minutes each ...
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bioRxiv - Neuroscience 2023Quote: ... along with DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride, Millipore-Sigma # 10236276001, used at 1 μg/mL), washed twice with PBSX for 15 minutes each ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear DNA was stained with DAPI (4′,6-diamidino-2-phenylindole, 1 mg/ml, Sigma-Aldrich, 10236276001).
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bioRxiv - Bioengineering 2024Quote: ... Cell nuclei were stained with 1 μg/mL 4′,6-diamidino-2-phenylindole (DAPI, D9542, Sigma Aldrich) for 10 min at RT before imaging ...
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bioRxiv - Molecular Biology 2024Quote: ... were washed three times with 3 ml of water using Amicon Ultra-4 (3 kDa cut-off, Millipore) (centrifugation at 4°C ...
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bioRxiv - Zoology 2023Quote: ... The concentrations used were 15.62 x 10-3 mg and 31.25 x 10-3 mg of capsaicin (CAS 4004-86-4 Sigma) per gram of body mass ...
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bioRxiv - Microbiology 2020Quote: ... After 23 h, cells were fixed with 4% paraformaldehyde and stained with DAPI (4’, 6-diamidino-2-phenylindole) (Sigma-Aldrich, USA). Nine fields per well were imaged on an Operetta high-content imaging system (PerkinElmer) ...
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bioRxiv - Bioengineering 2022Quote: ... the tissues were incubated overnight at RT and in dark conditions with a 1/400 dilution of Myosin 4 Monoclonal Antibody (MF20; eBioscience) as primary antibody and 4′,6-diamindino-2-phenylindole (DAPI; Sigma-Aldrich) at 1:5000 ...
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bioRxiv - Biophysics 2020Quote: ... pooled and concentrated to 2-4 mg/mL using Amicon Ultra-4 (100 kDa molecular weight cut off) concentrator tubes (EMD Millipore), flash frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Biophysics 2020Quote: ... MEFs transfected with LIM-protein plasmids were plated in 4-well Strex chambers (STB-CH-4W, 2 cm2 × 4 chambers) coated with 50 μg/mL fibronectin (Millipore, FC010), and allowed to recover overnight ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with 4% paraformaldehyde for 10 min and treated with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) diluted to a 1/10,000 concentration from the stock solution ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indoxyl phosphate (BCIP; Sigma Aldrich, Taufkirchen, Germany) for 5-30 min.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 8-(4-chlorophenylthio) adenosine-3’,5’-cyclic monophosphate (CPT-cAMP; PKA activator) or PMA (PKC activator)) were purchased from Sigma-Aldrich (ON, Canada). Powdered CBD ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The colorimetric signal was subsequently detected using nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP, Sigma-Aldrich, MO, USA) at 37 °C for a duration of 10 minutes.
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 10 μM DAPT (N-[N-(3, 5-difluorophenacetyl)-l-alanyl]-s-phenylglycinet-butyl ester) and 4 μM TAT-cre (Millipore, cat. no. SCR508) overnight.
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bioRxiv - Microbiology 2024Quote: ... Membrane protein topology was assayed by plating the resulting reporter strains on dual-indicator plates containing LB agar supplemented with 80 μg/ml 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos) ([Sigma Aldrich], RES1364C-A101X) and 100 μg/mL 6-chloro-3-indolyl-β-d-galactoside (Red-Gal ...
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bioRxiv - Cancer Biology 2024Quote: ... oral gavage for 21 days, using a 4-day-on, 3-day-off schedule) (Selleckchem, S7694), tamoxifen (20mg/kg, subQ, 5 doses/week) (Sigma Aldrich, T5648-1G), abemaciclib (75mg/kg ...
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bioRxiv - Biochemistry 2024Quote: ... Bands were visualized using the nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (BCIP/ NBT, Sigma-Aldrich, St. Louis, MO, USA) reaction ...
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bioRxiv - Immunology 2024Quote: ... followed by 4 - 5 hours of stimulation by phorbol myristate acetate (PMA, 5 ng/ml, Sigma) and ionomycin (500 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... 4 weeks after BM transplantation animals were injected with 5-FU (5-fluorouracil F6627, Sigma-Aldrich) at a dose of 150 mg/kg in 100ul of PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The [5′-32P] cytidine 3′,5′-bisphosphate was prepared by incubating 1 mM cytidine 3′-monophosphate (Sigma) with 312.5 pmole [γ-32P] ATP (6000 Ci/mmol ...
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bioRxiv - Cell Biology 2021Quote: ... samples were treated with 5 mM Methyl-β-cyclodextrin (Sigma-Aldrich) diluted in 10 μM Lovastatin (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... were purchased from Sigma-Aldrich and solved in 5% (w/v) methyl-β-cyclodextrin (Sigma Aldrich).
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bioRxiv - Immunology 2022Quote: ... mBECs were pretreated with 5 nM methyl-ß-cyclodextrin (Sigma C4555) for 1 hr prior to incubation with CD4+ T cells ...
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bioRxiv - Cancer Biology 2022Quote: Human melanoma cells were seeded in 6-well plates with 0.1-1 mL lentivirus in a final volume of 3 mL with 4 µg/mL polybrene (Millipore). Cells were spun at 1,000 x g for 2 h at 30 ºC ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were concentrated to ∼ 3-4 mg mL-1 using a 100 kDa MWCO centrifugal concentrator (Millipore Sigma) for cryo-EM sample preparation.
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bioRxiv - Cell Biology 2022Quote: ... Tubulin [B-5-1-2] (Sigma, 1:5000), Tim23 (BD Transduction Labs ...
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bioRxiv - Cell Biology 2023Quote: ... Tubulin [B-5-1-2] (Sigma, 1:5000), HSP60 [LK1] (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... containing 5 μM DAPT and 4 μM SU5402 (both from Sigma-Aldrich) for additional 3 days ...
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bioRxiv - Neuroscience 2020Quote: ... 5 dpf larvae were anesthetized using 4%Tricaine (42 mg/L, Sigma) 30 min before injections ...
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bioRxiv - Cell Biology 2022Quote: ... and finally mounted using Mowiol 4-88 (Sigma-Aldrich, 9002-89-5) mounting medium after several PBST washes ...