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Citations for Millipore Sigma :
901 - 950 of 10000+ citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... A standard stock solution containing 1% 2-methyl pentanoic acid (Sigma-Aldrich St. Louis, MO) was prepared as an internal standard control for the extractions ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin (B-5-1-2) (1:10000; Sigma-Aldrich T5168, B-5-1-2), mouse anti-GAPDH (1:25000 ...
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bioRxiv - Biochemistry 2020Quote: ... HLCs were exposed to either 5-Aminoimidazole-4-carboxamide-1-β-D-ribofuranosyl 5′-monophosphate (AICAR; 1mM; Sigma-Aldrich, A1393-50MG), O-(Carboxymethyl)hydroxylamine hemihydrochloride (AOA ...
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bioRxiv - Neuroscience 2020Quote: ... counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10000; D9542, Sigma-Aldrich, St Louis, USA), and mounted on glass slides with Mowiol 4-88 (Merck Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF) and 1x PhosSTOP phosphatase inhibitor cocktail (Sigma-Aldrich), and centrifuged at 10,000 x g for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclear DNA was stained with DAPI (4’,6-diamidino-2-phenylindole) (1 mg/ml) (Sigma-Aldrich).
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bioRxiv - Immunology 2022Quote: ... The slides were then stained with 1/1000 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) for 10 minutes at room temperature and washed twice for 5 minutes in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 min ...
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bioRxiv - Microbiology 2020Quote: ... and 0.5 µg ml-1 of 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich Chemie GmbH).
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bioRxiv - Cell Biology 2021Quote: ... paraformaldehyde (PFA) and 4-(2-hydroxyethyl)-1-piperazine-ethane-sulfonic acid (HEPES) were purchased from Sigma Chemical (St ...
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bioRxiv - Cell Biology 2022Quote: ... Cell nuclei were stained with 10.9 μM 4′,6-diamidino-2-phenylindole (DAPI, 1:1000 Sigma). Coverslips were mounted in ProlongGold (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was counterstained with 1 μg/mL DAPI (4’,6’-diamino-2-fenil-indol; Sigma-Aldrich) and mounted onto glass slides with 20 mM Tris pH8 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell nuclei were counter-stained with 4’,6-diamino-2-phenylindole (DAPI, Sigma-Aldrich, 1:1000).
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bioRxiv - Neuroscience 2023Quote: ... slides were incubated in 4′,6-diamidino-2-phenylindole (1:1000, DAPI, Sigma-Aldrich, Cat # D9542) solution for 10 mins ...
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bioRxiv - Cell Biology 2023Quote: ... for 10 min before being incubated with 1:1000 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) and 1:200 Phalloidin Alexa-647 (Stratech ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated with 4’,6-diamidino-2-phenylindole (DAPI; 1:5,000; Merck Millipore) and covered with Mowiol (Merck Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... Cell nuclei were counter-stained with 4’,6-diamino-2-phenylindole (DAPI, Sigma-Aldrich, 1:1000). For the DAPI staining ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked overnight at 4°C in 5% BSA (Sigma) then probed using pERK and tERK antibodies (Cell signalling ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were concentrated using Amicon Ultra-4 5 kDa (Millipore). Antibody concentration was determined by absorbance measurement at 280 nm using a Nanodrop and purity was determined using SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... SUPELCOSIL LC18 (30 cm x 4 mm, 5 μm, Sigma-Aldrich), or an Eclipse XDB-C18 column (150 mm x 4.6 mm ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were labeled with 5 mM 4-Thiouracil (Sigma-Aldrich) for 5 min ...
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bioRxiv - Immunology 2020Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) for CSR to IgG1 ...
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bioRxiv - Immunology 2024Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) (L-I) ...
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bioRxiv - Genomics 2023Quote: ... pombe cells were labeled with 5 mM 4-thiouracil (Sigma-Aldrich) for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP; 5 µM, SML2959, Sigma-Aldrich), and Rotenone (1 µM ...
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bioRxiv - Cancer Biology 2022Quote: ... The gelatin was subsequently cross-linked using N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC hydrochloride) (Sigma Aldrich Catalog No-03450) and N-Hydroxysuccinimide (NHS ...
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bioRxiv - Neuroscience 2021Quote: ... and then supplemented with 2% w/v of 2-Hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Sigma Aldrich 410896) as the UV initiator ...
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bioRxiv - Microbiology 2020Quote: ... ACE2 (15 μg/mL) was immobilized on AR2G biosensors activated by 1-ethyl-3-[3-dimethylaminopropy] carbodiimide hydrochloride (EDC, E1769, Sigma, Shanghai, CHN) and sulfo-N-hydroxysulfosuccinimide (s-NHS ...
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bioRxiv - Cell Biology 2024Quote: ... MICAL1#2 (5′CUCGGUGCUAAGAAGUUCU[dU][dU]3′) (75) and Rab35 (5′GCUCACGAAGAACAGUAAA[dU][dU]3′) (76) were synthetized by Sigma.
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bioRxiv - Immunology 2021Quote: Three- to 4-week-old mice were injected intraperitoneally twice with 0.25 mg 5-ethynyl-2’-deoxyuridine (EdU; Sigma-Aldrich) 2 hours apart ...
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bioRxiv - Genomics 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (5 μM) and propidium iodide (PI) (50 μg/mL) (Sigma-Aldrich, St. Louis, USA) and incubated at room temperature for 30-60 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed once with PBS prior incubation for 5 min at 37 °C with 4′,6-diamidino-2-phenylindole (DAPI; Sigma). Finally ...
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Systemic administration of Ivabradine, an HCN channel inhibitor, blocks spontaneous absence seizuresbioRxiv - Neuroscience 2021Quote: ... and ELA (N-[4-[2-(3,4-Dihydro-6,7-dimethoxy-2(1H)-isoquinolinyl)ethyl]phenyl]-9,10-dihydro-5-methoxy-9-oxo-4-acridinecarboxamide) were purchased from Sigma-Aldrich. For systemic injections ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Chromosomes were stained with 4′,6-diamidino-2-phenylindole at a concentration of 5 μg/ml (DAPI; Sigma-Aldrich, D8417).
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bioRxiv - Neuroscience 2024Quote: ... then incubated at 4°C for 1-3 days in the presence of Triton X-100 (0.3%, Sigma), 1% normal donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2020Quote: ... then rinsed 3 times in PBS (5 minutes each) and embedded in 4% low gelling temperature agarose in PBS (Sigma-Aldrich A0701). 55 μm slices of the ovaries were cut with a vibratome (Leica VT 1000 S ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed at 4°C for 3 h using 5 µg of an anti-FLAG M2 antibody (Sigma-Aldrich, F1804, mouse). For RIP-Seq ...
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bioRxiv - Molecular Biology 2020Quote: ... The bound probe was visualized using 5-bromo-4-chloro-3 indoyl phosphate (NBT/BCIP; Sigma-Aldrich Corp., St. Louis, MO, USA). Bright field images were captured on the A1+ Nikon confocal microscope fitted with a DS-Ri1-U3 color camera at 20x magnification and 4076×3116 pixel resolution ...
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bioRxiv - Plant Biology 2020Quote: ... Detection was performed by adding 200 μl of western blue substrate (SIGMAFAST™ BCIP®/NBT = 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium, Sigma) followed by incubation at room temperature in a dark and moist environment until the reagent’s color became visible ...
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bioRxiv - Neuroscience 2023Quote: ... Fixed cells were incubated with staining solution (1mg/mL of X-Gal,5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside; Sigma Aldrich #11680293001)(Lorenzo ...
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bioRxiv - Bioengineering 2023Quote: ... 5-Aminolevulinic acid hydrochloride >98% (5-ALA) (Sigma Aldrich, St. Louis, Missouri, United States) was used for cell dosing alongside standard GBM media ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: Cells were seeded on coated glass cover (Assistent) slips 2 days before fixation with 4 % paraformaldehyde (Sigma, 30525-89-4) for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... After addition of 250 µL 4× lysis buffer (4% SDS, 40 mM tris(2-carboxyethyl)phosphine (TCEP; Sigma-Aldrich, C4706), 160 mM chloroacetamide (CAM ...
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bioRxiv - Cell Biology 2024Quote: ... and then seeded at 2000 cells per well in enriched pseudoislet media supplemented with 2 μM 4-hydroxytamoxifen (4-OHT; Sigma) or vehicle control (0.1% ethanol ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded in a 96-well plate at a density of 15,000 cells per well in 50 µL of medium containing the HDAC substrate N-(4-Methyl-7-aminocoumarinyl)-Nα-(t-butoxycarbonyl)-Nω-acetyllysineamide (21 μM, MAL, Sigma-Aldrich) and incubated for 8 h at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... the acid insoluble fraction was incubated for 18 hours at 4°C with 500 μL of 6 M guanidine hydrochloride/100mM TRIS buffer (pH 7.4, Sigma-Aldrich, U.K.). The buffer was exchanged into 100 μL of 50 mM ammonium acetate (Scientific Laboratory Supplies ...
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bioRxiv - Genomics 2023Quote: ... total cellular ERAP2 protein was enriched by immunoprecipitation and incubated with L-Arginine-7-amido-4-methylcoumarin hydrochloride (R-AMC, A2027, Sigma-Aldrich). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... 1% 2-ethyl-3-methylpyrazine (Sigma W315508), 1% 2,5-dimethylpyrazine (Sigma 17542015) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1% phosphatase inhibitor cocktail 2 & 3 (Sigma) and protease inhibitor cocktail ...
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bioRxiv - Microbiology 2023Quote: ... 2’,3’,5’-Triacetyl-6-azauridine (azaribine; Sigma-Aldrich, Cat. # T340057), and brequinar sodium salt hydrate (brequinar ...