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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The protein was concentrated with an Amicon Ultra-4 (Merck Millipore, MWCO 3 K) to a concentration of 726 µM ...
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bioRxiv - Cancer Biology 2024Quote: ... 3-4-week-old mice were administered three subcutaneous injections of tamoxifen (Sigma Aldrich) in corn oil over 5 days (days 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 4×108 cells were fixed with 3% paraformaldehyde (pFA; Sigma, P6148) at the culture temperature for 10 minutes and disrupted with Mini-Beadbeater-16 (BioSpec Products ...
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bioRxiv - Developmental Biology 2020Quote: ... the nuclei were labeled with a 1:10,000 dilution of DAPI (4’,6-Diamidino-2-phenylindole; Sigma, D9542) in PBS ...
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bioRxiv - Plant Biology 2021Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich catalogue no D9542, 1:600 dilution). Figure 4 shows images of seedlings from 1 of 6 experiments ...
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bioRxiv - Cell Biology 2020Quote: ... nuclei were stained by addition of 1 µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich) containing FluoromountG (Southern Biotech) ...
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bioRxiv - Cancer Biology 2022Quote: ... the cover slides were treated with 1 mg/ml N-sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino) hexanoate (Sigma) and exposed to ultra-violet (UV ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by staining with 4’,6-diamidino-2-phenylindole (DAPI, 1:10,000 dilution with milliQ water, Sigma-Aldrich) for 10 minutes ...
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bioRxiv - Physiology 2020Quote: ... Nuclei were counter-stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 1/5000) (Sigma-Aldrich, Dorset, UK). Imaging was performed on a Leica TCS SP5 confocal microscope (Leica Microsystems ...
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bioRxiv - Immunology 2020Quote: Cell suspensions were stained for viability with either 1:3000 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) or 1:1000 Zombie Aqua (Biolegend ...
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bioRxiv - Synthetic Biology 2023Quote: ... and a titration of trimethoprim (0, 1, 2, 4, 8, 16, 24, and 32 µg/mL) (Sigma Aldrich) with 0.4 % dimethylsulfoxide ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) 1:1,000 (Sigma-Aldrich Sweden AB, Stockholm, Sweden). Invasion of the cells was measured by counting the nuclei of the cells that migrated through the filter pores towards the chemoattractant (6% FBS) ...
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bioRxiv - Neuroscience 2022Quote: ... with 1:30,000 of the fluorescent marker of nucleic acids 4’,6-diamino-2-fenilindol (DAPI; Sigma-Aldrich). We took photographs of the cells using an epifluorescence microscope (DMIL LED ...
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bioRxiv - Biochemistry 2022Quote: ... β-mercaptoethanol and HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid] were procured from Sigma (St. Louis, MO, USA). Urea and proteinase-K were bought from Amresco ...
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bioRxiv - Physiology 2022Quote: ... a 4 uL suspension of 2 μm red-fluorescent polystyrene microspheres (Sigma-Aldrich; 1:1000 dilution in PBS) was apically applied to native mucus ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were incubated with DAPI (4’,6-Diamidino-2-phenylindole dihydrochlorid) solution(D9542, Sigma-Aldrich, diluted 1:10000) for 7 minutes at RT and subsequently mounted in ProLong Gold Antifade mounting medium (cat ...
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bioRxiv - Pathology 2024Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, 1:10,000; Sigma-Aldrich; Saint Louis, MO, USA) and then ...
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bioRxiv - Bioengineering 2024Quote: ... was dispersed in 2 mL 4:1 (volume ratio) mixture solvent of cyclohexanone (Sigma-Aldrich, Product Number: 398241) and terpineol (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% (BC-1, BCBL-1) or 20% (BC-2, BC-3, BC-5, BJAB) Serum Plus-II (Sigma-Aldrich, catalog number 14009C ...
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bioRxiv - Bioengineering 2021Quote: ... 48.42 mg of N-(3-Dimethylaminopropyl)-N’-ehtylcarbodiimide hydrochloride (EDC-HCl) (Sigma-Aldrich) were added to the solution along with 27.40 mg of N-hydroxy-sulfosuccinimide (sulfo-NHS ...
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bioRxiv - Biophysics 2024Quote: ... and buffered with 2 mM N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid (TES, Sigma-Aldrich) at pH 7.4 (called Ham’s F12 observation medium).
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bioRxiv - Cell Biology 2020Quote: ... and Ypt1-7xHis were mixed in a 1:3 molar ratio and incubated overnight at 4°C with calf intestinal alkaline phosphatase (Sigma) to hydrolyze nucleotide ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) twice with 300 μL of Elution buffer (Lysis buffer A with 1 mg/mL 3 × FLAG peptide (Sigma)) ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation was performed at 4°C overnight with nutation in 1 ml chromatin aliquots with 3 ul of anti-FLAG M2 antibody (F3165, Sigma) and 20 ul of Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Physiology 2023Quote: ... Following overnight incubation at 4°C with primary antibodies against CARNS1 (rabbit polyclonal, 1:1000 in 3% milk/TBS-T, HPA038569, Sigma), membranes were washed (3 × 5 min) ...
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bioRxiv - Developmental Biology 2022Quote: ... Prior to start of imaging 6.5 ml culture medium with 1 μg/ml 17alpha,20beta-Dihydroxy-4-pregnen-3-one (DHP, diluted in 100% Ethanol, Sigma) containing drug/Lysotracker was added on top of the solidified LMP agarose ...
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bioRxiv - Biochemistry 2022Quote: ... Purified proMMP-3 and mutants were activated for 16 h in the presence of 1 mM 4-amino-phenyl mercuric acetate (Sigma) at 37 °C ...
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bioRxiv - Genetics 2023Quote: Resistance to chronic oxidative stress was measured by transferring 30 pre-fertile young adult worms to 60 mm NGM plates containing 4 mM paraquat (methyl viologen, Sigma Catalog No. 856177) and 100 μM FUdR and seeded with concentrated OP50 bacteria (see (Senchuk et al. ...
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bioRxiv - Neuroscience 2021Quote: ... a selective neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4) hydrochloride (Sigma, Dorset, UK) was used 3,4 ...
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bioRxiv - Systems Biology 2020Quote: ... for 1 – 4 hours at 4°C and subjected to IP overnight at 4C (0.3 µg H3K27me3 Millipore 07-449 ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells were treated for 30 min at 4°C in fixation buffer (4% formaldehyde (Sigma #47608)/5 mM EDTA ...
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bioRxiv - Genomics 2024Quote: ... samples were embedded in a thin polyacrylamide film by inverting them onto a GelSlick-coated microscope slide with a droplet of 4% acrylamide solution (4% v/v 20:1 acrylamide:bis-acrylamide [BioRad, 1610144] with 0.15% v/v TEMED [Sigma, T7024] and 0.30% v/v ammonium persulfate [Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µM 5-fluoro-2’-deoxyuridine (Sigma), penicillin ...
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bioRxiv - Cell Biology 2021Quote: ... alpha-tubulin (B-5-1-2, Sigma), tyrosinated tubulin (YL1/2 ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... and viral envelope (pMD2G) constructs at a 4:2:1 ratio with 1 mg/mL polyethylenimine (PEI, linear MD 25,000 Da, Sigma, Cat# 408727). The ratio of total transfected DNA to PEI was 1:3 (1 µg DNA:3 µg PEI) ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were washed for 1 h with PBX and stained with 1 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI) (SIGMA, USA) for 10 min in PBX ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 h at room temperature simultaneously with 4′,6-diamidino-2-phenylindole (DAPI: 1:2500, D9542; Sigma-Aldrich-Aldrich, USA) and Phalloidin Alexa488 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed twice at RT with 0.1% Triton 100X in PBS and then counterstaining with 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI) (1:5000, Sigma Millipore, #MBD0015) in PBS for five minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were washed twice with PBS and counterstained with 1 µg mL-1 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) to distinguish between live and dead cells ...
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bioRxiv - Developmental Biology 2024Quote: ... Worms were then washed 2x in PBSTx and counterstained via a 20-min incubation in 1:1000 4′,6-diamidino-2-phenylindole (DAPI, 1 mg/ml, Sigma Aldrich) in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... These were mounted in a drop of in 0.1 M phosphate buffer containing 1:1 of 2% methyl cellulose (Sigma-Aldrich) and 2.3 M sucrose on TEM grids with a carbon-coated Formvar film (Taab Laboratory Equipment Ltd) ...
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bioRxiv - Developmental Biology 2021Quote: ... 70nm Sections were picked up using a 1 :1 mixture of 2.3M sucrose and 2% methyl cellulose (cat# M-6385, Sigma-Aldrich) as a pick-up solution and sections were transferred to carbon and formvar coated mesh grids (cat# H100-Cu ...
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bioRxiv - Bioengineering 2022Quote: ... crosslinking using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Carl Roth GmbH + Co. KG) and N-hydroxysuccinimide (NHS) (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) was applied ...
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bioRxiv - Neuroscience 2021Quote: Brain samples were embedded in 4-5% agarose (Sigma-Aldrich: 9012-36-6) in 0.1M PB and imaged using serial two-photon tomography (Han et al 2018 ...
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bioRxiv - Microbiology 2023Quote: ... non-metabolizable 4 kDa FITC-labeled dextran (FD4; 5 mg/ml, Sigma-Aldrich) was added with bacteria (MOI ...
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bioRxiv - Bioengineering 2023Quote: ... Lyophilized PEG-OH (5 g) and 4-Dimethylaminopyridine (DMAP, 0.002 mol; Sigma-Aldrich) were dissolved in anhydrous dichloromethane (DCM ...
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bioRxiv - Microbiology 2024Quote: ... Metacyclic promastigotes were isolated from 4-5 day old cultures using Ficoll (Sigma) gradient separation for infections (23).
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bioRxiv - Immunology 2024Quote: ... 4×105 cells were incubated with 5 µg/ml of Hoechst 33342 (Sigma) at 37°C for 45 min ...
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bioRxiv - Cancer Biology 2024Quote: The siRNA cocktails containing 4-5 constructs against NAPRT were purchased from Sigma. Cells were transfected with silencing siRNA or non-targeting control siRNA for 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Tubulin (B-5-1-2, Sigma Aldrich, 1:10,000) and subsequently with HRP-tagged secondary antibodies (Jackson Immunoresearch ...