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Citations for Millipore Sigma :
1001 - 1050 of 4915 citations for Recombinant Human CTLA4 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... recombinant protein was induced with 1 mM IPTG (Sigma – Aldrich) and the culture was transferred to 25°C and 250 rpm for 5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... active recombinant proteins were purchased from Sigma (SRP0364, P0066, respectively). Recombinant purified human MASTL protein was used as a substrate and was from Abnova (H00084930) ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant ubiquitin was purchased from Sigma Aldrich (79586-22-4).
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant CDK5/p25 (60 ng) (EMD Millipore, Burlington, MA, USA) was combined with purified ACSS2 (2ug ...
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bioRxiv - Genetics 2022Quote: ... and 10 ug/L recombinant leukemia inhibitory factor (LIF) (Millipore). Mouse embryo stem cells was cultured at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... 25 ng/ml recombinant basic fibroblastic growth factor (FGF, Millipore), and 100 U/mL of penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant proteins were expressed in either BL21 or Rosetta (Novagen) cells ...
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bioRxiv - Molecular Biology 2021Quote: Recombinant proteins were expressed in Escherichia coli RosettaTM (DE3) (Millipore) by induction overnight with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich), and 0.5 μg/ml anti-CD180 (RP/14 ...
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bioRxiv - Immunology 2020Quote: ... then stimulated with 100U/mL recombinant murine IFNβ (Sigma, I9032) or equivalent volume of water for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... 10 µl recombinant RNase inhibitor (1 U/µl, Sigma-Aldrich) was added to 390 µl internal solution to increase RNA yield ...
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bioRxiv - Synthetic Biology 2023Quote: ... 103 units/mL ESGRO Recombinant Mouse LIF Protein (Millipore #ESG1107), 3.0 µM CHIR99021 (GSK-3 inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: ... LPA and recombinant Src protein were purchased from Sigma-Aldrich, while recombinant human IDO1 protein was obtained by Giotto Biotech ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/ml mouse recombinant IL-4 (Sigma-Aldrich) to allow B cell activation and class switch recombination to IgG1 ...
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bioRxiv - Bioengineering 2023Quote: ... recombinant insulin-like growth factor (R3-IGF) (Sigma Aldrich, #85580C), heparin (Sigma Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... All recombinant plasmids were transformed into E.coli Rosetta (DE3) (Novagen) cells and grown in LB broth at 37°C for 3 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 1000 U/ml ESGRO recombinant mouse LIF protein (Sigma ESG1106), 2 μM PD0325901 (Selleckchem S1036 ...
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bioRxiv - Cell Biology 2024Quote: ... and recombinant protein production was induced via IPTG induction (Sigma). Cells were lysed with BugBuster HT (Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant AMPK (α1, β1, and γ1, 0.5 ng/µL, Sigma), purified truncated ACC (250 ng/µL) ...
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bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
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bioRxiv - Plant Biology 2019Quote: ... Immuno-blotting was performed using anti-ubiquitin antibody and anti-His (Sigma, USA).
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bioRxiv - Biochemistry 2020Quote: 25 μl of the monoclonal α-His antibody (Catalog-ID H1029, Sigma-Aldrich) were mixed with 100 μl buffer comprising 50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2021Quote: Ezrin constructs in pET-28a(+) (N-terminal hepta-His tag, Novagen, Madison, WI): hEzrin wildtype (His-Ezrin/WT ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... SC –leu-trp plates and SC –leu-trp-his + 10mM 3-aminotriazole (Sigma) plates ...
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bioRxiv - Neuroscience 2020Quote: ... and HIS dihydrochloride (Sigma-Aldrich; 10 μg/10 μl; 9 mM; 90 nmol), as well as a combination of 2 substances (ALA 90 μg with BAM8-22 1 μg in 10 μl ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... which coeluted with His-TrxA or monoclonal anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich) for the detection of recombinant His-tagged TrxA ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with 15 ml HIS-Select Nickel Affinity Gel (Sigma) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Immunology 2024Quote: ... 10X hi ovomucoid inhibitor stock solution contained 300 mg BSA (Sigma-Aldrich, #A8806), 300 mg ovomucoid trypsin inhibitor (Worthington ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
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bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Genetics 2024Quote: ... supplemented with 10% Heat Inactivated Fetal Bovine Serum (HI-FBS) (Sigma, REF-F4135), 1% L Glutamine 200 mM (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... The HA-tagged PGBD1 was eluted by 500 μl 100 μg/ml HA-peptide (Sigma) in 100 mMNaCl ...
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bioRxiv - Cell Biology 2019Quote: Immunoprecipitations of Flag-tagged proteins were performed using α-Flag M2 affinity beads (Sigma-Aldrich). For each immunoprecipitation reaction ...
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bioRxiv - Cell Biology 2019Quote: ... Washed cells were treated with TRITC tagged Concanavalin A (20 µg ml-1) (Sigma, USA) for 1 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). All immunoprecipitations were performed on three independent biological replicates.
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bioRxiv - Cancer Biology 2021Quote: ... All FLAG-tagged proteins were purified with anti-FLAG® M2 Affinity Gel (Sigma-Aldrich) in a native condition using the FLAG peptide elution ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunoprecipitation of Flag-tagged UNC-3 was performed using Flag antibody coated beads (Sigma, #A2220). For the IP ...
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bioRxiv - Microbiology 2020Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously (49).