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Citations for Millipore Sigma :
851 - 900 of 4915 citations for Recombinant Human CTLA4 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Myc-tagged proteins were incubated with an anti-Myc primary antibody 9E10 (Millipore) at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP-tagged proteins were detected with peroxidase–anti-peroxidase– soluble complex (P1291, Sigma). α-tubulin was detected with antibody T5168 (Sigma).
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bioRxiv - Cell Biology 2020Quote: ... Myc-tagged proteins were immunoprecipitated with 4AG Myc monoclonal agarose beads (EMD Millipore), washed three times in lysis buffer and boiled in 2X Laemmli reducing buffer (Bio-Rad) ...
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bioRxiv - Physiology 2020Quote: ... or mouse anti-S-tag (for S-tagged NTD, 1:1500, Sigma, SAB2702204) and rabbit anti-Nav1.5 (1:150 ...
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bioRxiv - Immunology 2020Quote: ... Flag-tagged proteins were immunoprecipitated with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich). For the endogenous interaction assay ...
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bioRxiv - Molecular Biology 2021Quote: ... Epitope tagged PARP1 was detected by mouse anti-FLAG antibody (# F3165, Millipore-Sigma) and visualized with an anti-mouse IgG coupled with Alexa Fluor 594 dye (Thermo Fisher Scientific # A-11005) ...
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bioRxiv - Immunology 2022Quote: ... GFP-tagged SAMHD1 proteins were immunoprecipitated with anti-SAMHD1 antibody (Sigma-Aldrich, SAB1400478) bound to Protein-A/G-Agarose (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged proteins were detected by FLAG-HRP conjugated antibody (Sigma, A8592, 1:4000), mouse HA-probe antibody (Thermofisher ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged protein was isolated with anti-FLAG M2 magnetic beads (Sigma, M8823) for 2 hr at 4LJ°C and eluted with 100 μg/ml 3x FLAG peptide ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged proteins were purified using anti-FLAG M2 Affinity agarose gel (Sigma) for >1h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Flag-tagged proteins were bound to anti-FLAG M2 affinity gel (Sigma-Aldrich) for 1 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected using anti-FLAG M2 primary (Sigma-Aldrich, F1804) antibodies combined with anti-mouse-HRP secondary (Rockland ...
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bioRxiv - Plant Biology 2024Quote: ... HA-tagged proteins were detected by anti-HA Peroxidase high affinity antibody (Sigma). GFP was detected using a horseradish peroxidase-conjugated anti-GFP antibody (1:1.000 ...
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bioRxiv - Cell Biology 2024Quote: ... and FLAG tagged proteins were immunoprecipitated using anti-FLAG M2 affinity gel (Sigma). Elution was carried out with 2X Bolt LDS sample buffer and the samples were blotted onto the PVDF membrane ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged proteins were pulled down using anti-FLAG M2 magnetic beads (Sigma) and serially diluted with enzyme buffer before ATP and fluorescein-conjugated PKC substrate peptide (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
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bioRxiv - Immunology 2020Quote: NTD-Fc or RBD-Fc proteins were diluted in sterile PBS and formulated as an emulsion with TiterMax Gold adjuvant (Sigma-Aldrich, St. Louis, MO) to a final antigen concentration of 250 µg/mL ...
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bioRxiv - Immunology 2022Quote: ... For surface staining cells were incubated with mastermix prepared in Fc-block (1%FCS/PBS with 0.1% rat IgG (Sigma-Aldrich, St. Louis, MO, USA)) ...
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bioRxiv - Developmental Biology 2019Quote: ... supplemented with 10% FCS and 1% penicillin and streptomycin (Sigma). After separation from UGE ...
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bioRxiv - Genetics 2021Quote: ... supplemented with 1 g/ml glucose containing 10% FCS (Sigma), 1% penicillin (10,000 units/ml ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 10% inactivated Fetal Calf Serum (FCS) (Sigma-Aldrich), 100 ng ml−1 L-Glutamine ...
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bioRxiv - Biochemistry 2020Quote: ... the MR-Fc was purified over mannan-agarose beads (Sigma) and protein A-agarose beads ...
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bioRxiv - Genetics 2022Quote: ... containing 10% of fetal calf serum (FCS) (Sigma-Aldrich, F7524), 1% of penicillin/streptomycin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... 2% FCS and 0.5µg/ml DAPI (Sigma Aldrich, Ref: D9542) and passed through a 40µm Falcon cell strainer ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% FCS (Sigma-Aldrich, #F9665-500ML, Lot #19A124) and 1% Penicillin-Streptomycin-L-Glutamine (MED30-009-CI) ...
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bioRxiv - Microbiology 2021Quote: ... 4% (v/v) heat-inactivated foetal calf serum (FCS) (Sigma), 25 mM HEPES buffer (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... 20% FCS and 1% L-glutamine (Sigma-Aldrich, Buchs, Switzerland), as described in (41) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5% v/v heat inactivated FCS (Sigma Aldrich cat. # 12133C) and Penicillin-Streptomycin (100 units/mL) ...
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bioRxiv - Microbiology 2020Quote: ... 4% (v/v) heat-inactivated foetal calf serum (FCS) (Sigma) and 25mM HEPES buffer (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... containing 10 % fetal calf serum (FCS) (Sigma-Aldrich, Hamburg, Germany) and 1 % penicillin / streptomycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... complemented with 10 % fetal calf serum (FCS; Sigma-Aldrich F7524) and 1 % of penicillin and streptomycin mixture (Sigma-Aldrich P0781 ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10 % fetal calf serum (FCS, Sigma-Aldrich F7524) and 1 % penicillin streptomycin mixture (Sigma-Aldrich P0781) ...
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bioRxiv - Immunology 2021Quote: ... and fetal calf serum (FCS; 20% v/v; Sigma-Aldrich). Complete medium (R+ ...
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bioRxiv - Immunology 2021Quote: ... counted and cryopreserved in 90% FCS / 10% DMSO (both Sigma). PBMC aliquots were frozen in a methanol bath (to freeze slowly ~ 1C/min ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 8% (v/v) Fetal Calf Serum (FCS; Sigma), penicillin (100 U mL-1) ...
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bioRxiv - Neuroscience 2023Quote: ... These specimens were immersed in Fluorinert (FC-40, Sigma-Aldrich) and allowed to rest for four hours at room temperature before scanning ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% FCS (Biowest) and 1% Pen/Strep (Sigma).
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bioRxiv - Cancer Biology 2024Quote: ... and fetal calf serum (10%, FCS, Sigma-Aldrich-Chemie-BV)) with Geneticin G418 Sulphate (0.6 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% fetal calf serum (FCS) (Sigma Aldrich, F7524) and maintained in a humidified incubator at 37°C and 5% CO2.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma F-7524), 1000 U/mL LIF ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was additionally supplemented with 2.5% FCS (Sigma Aldrich) from DIV 9 onwards ...
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bioRxiv - Immunology 2023Quote: ... and 8% fetal calf serum (FCS, Sigma-Aldrich Chemie BV). TK-1 murine T-cell lymphoma cells (CRL-2396 ...
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bioRxiv - Immunology 2023Quote: ... complete media supplemented with 10% (vol/vol) FCS (F0804, Sigma), 1% penicillin-streptomycin (15140122 ...
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bioRxiv - Immunology 2023Quote: ... 70% fetal calf serum (FCS) (Sigma-Aldric, St. Louis, MO) and 20% RPMI 1640 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... U251 glioblastoma cells were purchased from Millipore Sigma and maintained in DMEM supplemented with 10% FCS (Sigma-Aldrich) and PenStrep (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma, F7524-500ML) and 1% Penicillin-Streptomycin (Pen/Strep ...
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bioRxiv - Biophysics 2023Quote: ... which consisted of fluorinated oil (Fluorinert FC-40, Sigma-Aldrich) and 2% w/w fluorosurfactant (RAN biotechnologies ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% Fetal Bovine Serum (FCS; Sigma-Aldrich, F7524) and 1% Penicillin-Streptomycin (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... with 0.75 U/kg of recombinant insulin (Sigma-Aldrich). Blood samples were collected from the tail vein and glucose was measured using a glucometer (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... 1000 U/mL recombinant leukemia inhibitory factor (LIF, Millipore), and 0.3% BSA (Gibco ...