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Citations for Millipore Sigma :
801 - 850 of 4638 citations for Recombinant Human CTLA4 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... EGFP-tagged TPX2 expressing hESCs with minimal dose of doxycycline (Sigma-Aldrich, #D9891) were selected using a cell sorter (BD FACSAria II) ...
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bioRxiv - Microbiology 2022Quote: ... GST-tagged proteins were purified under native conditions on gluthatione-agarose resin (Sigma). The recombinant proteins were then washed 3 times with Buffer A (100mM KCl ...
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bioRxiv - Biochemistry 2022Quote: ... and MBP-tagged and associated proteins were eluted in 60 mM maltose (Sigma) for 30 minutes on ice.
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bioRxiv - Cell Biology 2020Quote: The 6His-tagged-She1Cter expression plasmid for was constructed in pet28 vector (Novagen). The She1 C-terminal part coding for amino-acid 194 to 338 was cloned between the NdeI and XhoI site of the vector downstream of 6His and thrombin site.
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bioRxiv - Molecular Biology 2019Quote: ... Myc-tagged proteins were incubated with an anti-Myc primary antibody 9E10 (Millipore) at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP-tagged proteins were detected with peroxidase–anti-peroxidase– soluble complex (P1291, Sigma). α-tubulin was detected with antibody T5168 (Sigma).
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bioRxiv - Cell Biology 2020Quote: ... Myc-tagged proteins were immunoprecipitated with 4AG Myc monoclonal agarose beads (EMD Millipore), washed three times in lysis buffer and boiled in 2X Laemmli reducing buffer (Bio-Rad) ...
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bioRxiv - Physiology 2020Quote: ... or mouse anti-S-tag (for S-tagged NTD, 1:1500, Sigma, SAB2702204) and rabbit anti-Nav1.5 (1:150 ...
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bioRxiv - Immunology 2020Quote: ... Flag-tagged proteins were immunoprecipitated with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich). For the endogenous interaction assay ...
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bioRxiv - Molecular Biology 2021Quote: ... Epitope tagged PARP1 was detected by mouse anti-FLAG antibody (# F3165, Millipore-Sigma) and visualized with an anti-mouse IgG coupled with Alexa Fluor 594 dye (Thermo Fisher Scientific # A-11005) ...
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bioRxiv - Immunology 2022Quote: ... GFP-tagged SAMHD1 proteins were immunoprecipitated with anti-SAMHD1 antibody (Sigma-Aldrich, SAB1400478) bound to Protein-A/G-Agarose (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Flag-tagged proteins were bound to anti-FLAG M2 affinity gel (Sigma-Aldrich) for 1 h at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged proteins were purified using anti-FLAG M2 Affinity agarose gel (Sigma) for >1h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected using anti-FLAG M2 primary (Sigma-Aldrich, F1804) antibodies combined with anti-mouse-HRP secondary (Rockland ...
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bioRxiv - Plant Biology 2024Quote: ... HA-tagged proteins were detected by anti-HA Peroxidase high affinity antibody (Sigma). GFP was detected using a horseradish peroxidase-conjugated anti-GFP antibody (1:1.000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged proteins were detected by FLAG-HRP conjugated antibody (Sigma, A8592, 1:4000), mouse HA-probe antibody (Thermofisher ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged protein was isolated with anti-FLAG M2 magnetic beads (Sigma, M8823) for 2 hr at 4LJ°C and eluted with 100 μg/ml 3x FLAG peptide ...
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bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
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bioRxiv - Immunology 2020Quote: NTD-Fc or RBD-Fc proteins were diluted in sterile PBS and formulated as an emulsion with TiterMax Gold adjuvant (Sigma-Aldrich, St. Louis, MO) to a final antigen concentration of 250 µg/mL ...
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bioRxiv - Immunology 2022Quote: ... For surface staining cells were incubated with mastermix prepared in Fc-block (1%FCS/PBS with 0.1% rat IgG (Sigma-Aldrich, St. Louis, MO, USA)) ...
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bioRxiv - Physiology 2021Quote: ... with 0.75 U/kg of recombinant insulin (Sigma-Aldrich). Blood samples were collected from the tail vein and glucose was measured using a glucometer (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... 1000 U/mL recombinant leukemia inhibitory factor (LIF, Millipore), and 0.3% BSA (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... active recombinant GSK3α was purchased (Millipore, Cat# 14-492). 0.8 µg Dyn2WT or Dyn2S848A was incubated with 10 ng GSK3α in kinase buffer (50 mM HEPES (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... active recombinant GSK3α was purchased (Millipore, Cat# 14-492). 0.8 µg Dyn2WT or Dyn2S848A was incubated with 10 ng GSK3α in kinase buffer (50 mM HEPES (pH 7.4) ...
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bioRxiv - Neuroscience 2020Quote: ... brefeldin A and recombinant insulin were purchased from Sigma. NSC23766 was purchased from Santa Cruz ...
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bioRxiv - Bioengineering 2022Quote: ... Plates were coated with either recombinant GFP protein (Millipore) at 1 μg/mL to measure GFP-specific responses ...
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bioRxiv - Microbiology 2022Quote: ... recombinant IL-2 (100 U/mL; Sigma Millipore #11011456001), and anti-CD28 + anti-CD49d (1.5ug/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 μg/ml of recombinant TNC (Merck Millipore) added to the medium ...
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bioRxiv - Plant Biology 2021Quote: ... which yields recombinant proteins fused to hexahistidine tail (Novagen). The correct cloning was confirmed by sequencing.
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of recombinant CDK1:CyclinB1 active complex (Millipore) in the presence or absence of 3 mM ATP ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of recombinant CDK1:CyclinB1 active complex (Millipore) in the presence or absence of 3 mM ATP ...
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bioRxiv - Microbiology 2022Quote: ... recombinant IL-2 (100 U/mL; Sigma Millipore #11011456001), and anti-CD28 + anti-CD49d (1.5ug/ml ...
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bioRxiv - Biochemistry 2022Quote: ... 1,000 U/mL recombinant leukemia inhibitory factor (LIF, Millipore), and 0.3% BSA (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant mouse EGF (0·025 µg/ml, Sigma-Aldrich), and bovine pituitary extract (30 µg/ml ...
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bioRxiv - Immunology 2024Quote: ... 20 μg of Ovalbumin recombinant protein (Sigma-Aldrich, A5503) (1:1 mixed with Alhydrogel ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... supplemented with 75 mIU/ml of recombinant FSH (Millipore), 100 mIU/ml recombinant hCG (Sigma) ...
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bioRxiv - Developmental Biology 2019Quote: ... supplemented with 10% FCS and 1% penicillin and streptomycin (Sigma). After separation from UGE ...
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bioRxiv - Genetics 2021Quote: ... supplemented with 1 g/ml glucose containing 10% FCS (Sigma), 1% penicillin (10,000 units/ml ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 10% inactivated Fetal Calf Serum (FCS) (Sigma-Aldrich), 100 ng ml−1 L-Glutamine ...
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bioRxiv - Biochemistry 2020Quote: ... the MR-Fc was purified over mannan-agarose beads (Sigma) and protein A-agarose beads ...
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bioRxiv - Genetics 2022Quote: ... containing 10% of fetal calf serum (FCS) (Sigma-Aldrich, F7524), 1% of penicillin/streptomycin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... 2% FCS and 0.5µg/ml DAPI (Sigma Aldrich, Ref: D9542) and passed through a 40µm Falcon cell strainer ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% FCS (Sigma-Aldrich, #F9665-500ML, Lot #19A124) and 1% Penicillin-Streptomycin-L-Glutamine (MED30-009-CI) ...
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bioRxiv - Microbiology 2021Quote: ... 4% (v/v) heat-inactivated foetal calf serum (FCS) (Sigma), 25 mM HEPES buffer (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... 20% FCS and 1% L-glutamine (Sigma-Aldrich, Buchs, Switzerland), as described in (41) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5% v/v heat inactivated FCS (Sigma Aldrich cat. # 12133C) and Penicillin-Streptomycin (100 units/mL) ...
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bioRxiv - Microbiology 2020Quote: ... 4% (v/v) heat-inactivated foetal calf serum (FCS) (Sigma) and 25mM HEPES buffer (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... containing 10 % fetal calf serum (FCS) (Sigma-Aldrich, Hamburg, Germany) and 1 % penicillin / streptomycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... complemented with 10 % fetal calf serum (FCS; Sigma-Aldrich F7524) and 1 % of penicillin and streptomycin mixture (Sigma-Aldrich P0781 ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10 % fetal calf serum (FCS, Sigma-Aldrich F7524) and 1 % penicillin streptomycin mixture (Sigma-Aldrich P0781) ...