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Citations for Millipore Sigma :
9951 - 10000 of 10000+ citations for Mouse FAM117A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Sera were incubated for one hour at room temperature followed by three washes with PBST before incubating with an anti-mouse IgG secondary antibody conjugated to alkaline phosphatase (Sigma) used as a 1:5000 dilution for one hour ...
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bioRxiv - Microbiology 2021Quote: Isotyping of the monoclonal antibodies and polyclonal sera responses was performed using the Mouse Monoclonal Antibody Isotyping Kit (Sigma-Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... Expression of GAPDH was used as a loading control using a mouse anti-GAPDH antibody (1:10000 dilution, MAB374, Sigma) and IRDye 680CW goat anti-mouse IgG secondary antibody (1:5000 dilution ...
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bioRxiv - Pathology 2021Quote: ... postmortem kidney was fixed in 20% formalin for 2 weeks and processed for free-?oating preparation as done for mouse tissue with one exception that anti-human CD31 antibody (CBL468, 1:20 dilution, Millipore) was used.
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bioRxiv - Systems Biology 2020Quote: ... Immunoprecipitation was carried out by incubating the lysate with the monoclonal anti-FLAG M2-mouse primary antibody (Sigma Aldrich, F1804) and protein G Dynabead (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... The following primary antibodies were used for the Western blotting: mouse antibodies against Flag (Sigma-Aldrich, St. Louis, MO, USA) and mouse antibody against β-actin (Proteintech Group ...
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bioRxiv - Cell Biology 2020Quote: ... and the mouse monoclonal and rabbit polyclonal anti-phosphorylated histone H3 (Ser10) antibodies (ref. 06570, 1:1000) were obtained from Millipore. The anti-GFP mouse monoclonal antibody mix (ref ...
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bioRxiv - Synthetic Biology 2020Quote: ... The membrane was then washed several times with Tris-Buffered Saline with 0.1% Tween 20 (TBST) before a 30 in incubation with monoclonal Mouse Anti-FLAG M2-Peroxidase (HRP) antibodies (Sigma-Aldrich) diluted 1:5000 in TBST ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for 24 hours at 4 °C in mouse anti-GAD67 (1:1000; Millipore Sigma, cat# MAB5406), rabbit anti-NeuN (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... GSTU5 was detected by the anti-GST primary antibody (Novex, 1:500) and anti-mouse secondary antibody (Sigma, 1:5000).
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bioRxiv - Immunology 2019Quote: ... 0.1% [w/v] saponin in PBS) for 25 min at RT and then stained with mouse anti-flavivirus antibody (clone D1-4G2-4-15, Millipore) diluted 1:500 in FACS wash buffer for 90 min at RT ...
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bioRxiv - Plant Biology 2019Quote: ... the blot was washed with 1×TBS-T solution (4×5min) and incubated with anti-Mouse IgG-HRP secondary antibody solution (Sigma, 1:10,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche] ...
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bioRxiv - Developmental Biology 2020Quote: HRP-conjugated secondary antibodies for Western blotting: goat anti-mouse IgG (Cat# A 4416, Sigma-Aldrich, St. Louis, MO, USA) and goat anti-rabbit IgG (Cat# GtxRb-003-EHRPX ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-HA (1:2000, Sigma); rabbit polyclonal MBP-probe (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Plates were washed twice with PBS and incubated in same conditions with horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (Sigma), diluted 1:1000 in blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The Flag-tagged receptor was detected using mouse anti-Flag M2 primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #F3165) and IRDye® 680RD-conjugated donkey anti-mouse IgG secondary antibody (1:20,000 dilution ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then treated with 5% BSA blocking solution for 1 h and the incubated with mouse monoclonal anti-HA (Sigma) antibody and rabbit anti-Flag antibody (Sigma ...
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bioRxiv - Immunology 2019Quote: ... Mouse neutrophil was isolated from the peritoneal cavity 3 hours after injection of 1 ml thioglycollate medium (Sigma-Aldrich, MO). Isolated neutrophils were incubated at 37C for 6 hours and then treated with 10 ng/ml ...
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bioRxiv - Physiology 2020Quote: ... and probed overnight (4 °C) with one of the following primary antibodies: Anti-TSG-6 mouse monoclonal (1 μg/ml; Millipore); rat monoclonal TNFα (1 μg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... and grown in standard human embryonic stem (ES) cells media supplemented with 6ng/ml basic Fibroblast Growth Factor (bFGF) on inactivated mouse embryonic fibroblast (MEFs) (Sigma) as feeder cells ...
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bioRxiv - Bioengineering 2019Quote: For Flag-tag detection membranes were incubated for two hours with the monoclonal anti-Flag M2 antibody from mouse (F3165, Sigma) in a 1:5000 dilution (in TBS-T ...
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bioRxiv - Physiology 2019Quote: Mouse islets were isolated as previously described61 using Type XI collagenase (1000 units/mL; #C7657, Sigma-Aldrich, St. Louis, MO). Following digestion and filtration ...
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... Plated were washed twice and incubated 1hr/37C/5%CO2/rocking with horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody (Sigma), diluted 1:1,000 in blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with the following primary antibodies for 1 h at room temperature in Abdil: mouse anti-human lamin A/C (1:200; Millipore), rabbit anti-lamin A/C (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... All single-cell samples were distributed at 0.5×106 cells/mL for live staining with monoclonal mouse anti-human FOLR1 IgG1 (LS Bio) in DPBS with 2% v/v FBS (Sigma). Secondary staining for target was performed using QIFIKIT® (BIOCYTEX ...
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bioRxiv - Cell Biology 2021Quote: ... and rabbit monoclonal anti-phospho-PKA substrate (clone 100G7E, CST, 1:1,000) and mouse monoclonal anti-phosphotyrosine (1 μg/ml; clone 4G10, Sigma Aldrich) and acetylated tubulin (1:20,000 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 h at 4 βC with primary antibodies (rat anti-BrdU, 1:500, OBT Serotec; mouse anti-NeuN, 1:200, MAB377, Millipore; rabbit anti-S100β ...
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bioRxiv - Developmental Biology 2021Quote: ... Antibodies used were chicken anti-GFP (1:10,000, Aves GFP-1010) and mouse anti-β-tubulin (1:5000, Sigma T4026).
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bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was incubated overnight at 4 °C with the first antibody (rabbit anti-Tc-Zen1 and anti-Tc-Zen2 as for immunohistochemistry, 1:1,000; mouse anti-Tubulin [Sigma-Aldrich #T7451 ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich) in PBST ...
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bioRxiv - Biochemistry 2021Quote: Antibodies used for Western Blot include mouse monoclonal anti β-actin (clone AC-15, Sigma, St. Louis, MO, United States) at 0.13 μg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... samples were boiled and resolved by SDS-PAGE followed by western blotting with mouse FLAG (1:2,500; F3165, Sigma-Aldrich), rabbit MYC (1:2,000 ...
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bioRxiv - Biochemistry 2021Quote: ... Inputs and eluates were separated by SDS-PAGE and transferred to nitrocellulose for Western blotting with mouse anti-FLAG (1:2,500; Sigma F3165) and rabbit anti-calnexin (1:3,000 ADI-SPA-865 ...
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bioRxiv - Immunology 2021Quote: ... Cytokines and chemokines in the homogenates were quantified using Luminex technology with a MILLIPLEX Map Mouse Cytokine/Chemokine Magnetic Bead Panel 25-plex Immunology Multiplex Assay (Cat. No. MCYTOMAG-70K-PMX, Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... standard Western blot was performed using anti-SST and anti-α-Tubulin (1:8,000, B-5-1-2, mouse monoclonal, Sigma).
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bioRxiv - Pathology 2021Quote: ... Splenocytes were isolated from wild-type mouse spleens and cultured overnight with 100ng/ml LPS and 5ug/ml PHA (Sigma). The cells were then labeled with cell trace violet (CTV ...
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bioRxiv - Cell Biology 2020Quote: ... slices from mouse brains 10 months (300 days) of age or older were stained with Thioflavine S (Thio-S, Sigma). Slices were washed with 0.1 M PB three times ...
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bioRxiv - Cell Biology 2021Quote: ... a mouse monoclonal antibody against a highly conserved epitope of chicken gizzard actin (clone C4) from EMD Millipore (Burlington, MA), and a mouse monoclonal antibody against the Ser19 phosphorylated form of the myosin II regulatory light chain (P-MyoRLC ...
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bioRxiv - Cell Biology 2020Quote: ... electroporated parasites were mixed immediately with 100 μl of reticulocyte-rich blood from a naïve mouse treated with phenylhydrazine (6 mg/ml) (Sigma-Aldrich), incubated at 37°C for 20 min and then injected intraperitoneally into another mouse ...
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bioRxiv - Cell Biology 2020Quote: ... Glycaemia was measured using a glucometer and insulin levels using the Milliplex Map Kit (Mouse Metabolic Magnetic Bead Panel. MMHMAG-44K, Millipore) Milliplex Map Kit ...
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bioRxiv - Cell Biology 2020Quote: ... were washed with 100 μl worm protein lysis buffer and incubated with monoclonal M2 mouse anti-Flag antibody (Sigma, F3165) or monoclonal rabbit anti-c-Myc antibody (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... after which bone marrow cells were collected and labeled with monoclonal anti-mouse CD3 (Sigma, catalog no. SAB4700050, 1: 1000), CD19 (Sigma ...
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bioRxiv - Genetics 2021Quote: ... were superovulated by serial Pregnant Mare Serum Gonadotropin (5 IU per mouse, Prospec Protein Specialists) and human chorionic gonadotropin (5 IU, Millipore) injections 46 h apart ...
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bioRxiv - Immunology 2020Quote: ... then binding was detected with specific alkaline phosphatase-conjugated secondary antibodies (anti-mouse total IgG and anti-monkey total IgG from Sigma and anti-mouse IgG1 and IgG2a from BD Biosciences) ...
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bioRxiv - Neuroscience 2020Quote: ... The mouse anti-GFP-20 antibody was used in GRASP and synaptotagmin experiments (1:100 in PBST-NGS; Sigma-Aldrich); the mouse nc82 antibody was used in synaptotagmin::GFP and DenMark experiments ...
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bioRxiv - Microbiology 2020Quote: ... From these fixed cells the manufacturer’s Duolink PLA Immunofluorescence protocol was followed closely using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Millipore Sigma). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... lysates were pre-cleared for 1 hour (h) with mouse immunoglobulin G (IgG) agarose or rabbit immunoglobulin G (IgG) agarose (Sigma), dependent on the species of antibody used in IP ...
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bioRxiv - Immunology 2020Quote: ... 8-µm-thick tissue sections were rehydrated and Fc-blocked with normal mouse and rat serums in the presence of 0.3% Triton X100 (Sigma-Aldrich). Tissues were stained with F4/80-APC (Clone BM8 ...
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bioRxiv - Molecular Biology 2021Quote: Total splenocytes were isolated from C57BL/6 and mFICD-/- mouse spleens and then treated for 3 days with 20 μg/ml LPS (Sigma), 100 µg/ml heparan sulfate or 2.5 μM thapsigargin (Enzo Life Sciences) ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated with mouse monoclonal Alexa Fluor-488 conjugated antibody against NeuN (1:200, MAB377X, Merck Millipore, MA, USA) or the rabbit polyclonal primary antibody against DISC1 (1:250 ...