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Citations for Millipore Sigma :
9701 - 9750 of 10000+ citations for Mouse FAM117A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... antibodies were purchased from Developmental Studies Hybridoma Bank. Mouse anti β-ACTIN (cat. A5316, RRID: AB_476743; 1:1,000 for IB) was purchased from Sigma. Goat anti-Mouse IgM (Heavy chain ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000, Sigma) and anti-c-Myc rabbit antibody clone 7E18 (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse zygotes were isolated in FHM under mineral oil and cumulus cells were removed with hyaluronidase (Sigma-Aldrich; H4272). Mouse embryos were cultured in pre-equilibrated Global Media (Cooper Surgical ...
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bioRxiv - Microbiology 2023Quote: ... spleen cells from a single mouse were harvested and stimulated for 6 h with 16 nM PMA (Sigma-Aldrich) and 1 μM ionomycin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit polyclonal anti-Laminin (1:50; Santa Cruzcat # sc20142) and mouse monoclonal anti-CSPG (1:200; Sigma cat # C8035). Phalloidin Alexa Fluor 594–conjugated (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... 1:500 dilution of rabbit anti-MeCP2 (Cell signalling) and 1:10000 dilution of mouse anti-vinculin (Sigma Aldrich). After the incubation with the primary antibody ...
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bioRxiv - Molecular Biology 2023Quote: PLA experiments were performed using the DuoLink In Situ Red Starter Kit Mouse/Rabbit (Millipore/Sigma-Aldrich # DUO92101-1KT) and following manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: PLA experiments were performed using the DuoLink In Situ Red Starter Kit Mouse/Rabbit (Millipore/Sigma-Aldrich # DUO92101-1KT) and following manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by application of a Cy3-conjugated goat anti-mouse antibody (1:250 dilution, #AP124C; Merck Millipore) as the secondary antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-mouse and anti-rabbit IgG horseradish peroxidase (HRP)-linked antibodies (Amersham) and streptavidin HRP-conjugated antibodies (Sigma-Aldrich) were used for ECL detection Reagents (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 1-5 mm3 biopsies from naked mole-rat or shaved mouse back skin were incubated with either: I) dissociation buffer (1.25 mg/ml collagenase I (Sigma), 0.5 mg/ml collagenase II (Worthington) ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-human-Dia2-isoform1 (gift from Véronique Pizon) and GFP-mouse-Dia2-isoform3 (gift from Yosuke Senju) using X-tremeGENETM HP DNA Transfection Reagent (Sigma) according to the manufacturer’s instructions cultured for a total of 2 days ...
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bioRxiv - Neuroscience 2023Quote: ... They were stained with 1/400 (v/v) of primary antibody mouse anti-rhodopsin clone 4D2 (MABN15, Sigma-Aldrich) and allowed to incubate for 30 min ...
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bioRxiv - Cell Biology 2023Quote: Coverslips with either adult mouse or neonatal rat cardiomyocytes were incubated 1h with anti-α-actinin antibody (A7811, Sigma) at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubating with the primary antibody (mouse monoclonal anti-acetylated tubulin, 6-11B-1, 1:1000, Sigma T6793) and then with the secondary antibody (goat anti-mouse IgG-Alexa Fluor 488 ...
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bioRxiv - Cancer Biology 2023Quote: ... in -20°C methanol for 10 minutesCells were stained with mouse anti-γ-tubulin 1:500 (Sigma Aldrich, T5326), rabbit anti-KIF18A 1:100 (Bethyl ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies (insulin rabbit monoclonal, Cell Signaling 3014, 1:3000 and glucagon, mouse monoclonal, Sigma-Aldrich G2654, 1:1000) were applied and incubated for 60 minutes followed by secondary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... the tissues were incubated with primary mouse anti–α-actinin (sarcomeric) antibody (1:750, Sigma-Aldrich, cat. no. A7811), and vimentin (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed in PBS-T (0.05% Tween-20 in PBS) and PBS before incubation with 1:10,000 dilution of HRP-conjugated anti-mouse IgG (Sigma) for 1 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... the cells were incubated in a 1:500 dilution of 4D2 mouse anti-PLSCR1 antibody (Millipore Sigma, cat. #MABS483) in PBS-BG (1x PBS with 0.5% bovine serum albumin and 0.1% glycine ...
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bioRxiv - Developmental Biology 2024Quote: Primary antibodies used in this study include: Phospho Histone H3 Ser10 Mitosis Marker mouse 1:500 (Millipore 06-570), MMP1 catalytic domain mouse 1 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-N protein (1:1000, CiteAb #40588-T62) and mouse anti-acetylated α-tubulin (1:200, Sigma-Aldrich #T7451). The secondary antibodies used were goat anti-mouse and goat anti-rabbit conjugated to Alexa Fluor 568 or 488 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed thrice with TBS-T buffer prior to addition of horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in blocking solution (anti-mouse-HRP 1:10,000 or anti-rabbit-HRP 1:10,000 antibodies; all from Sigma Aldrich) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubating with the secondary antibodies (anti-Mouse MINUS, DU092004, Sigma-Aldrich; anti-Rabbit PLUS, DUO92002, Sigma-Aldrich) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... then incubated for 1 hour with the appropriate peroxidase-conjugated anti-mouse secondary antibody at 1/5,000 (EMD Millipore). Immunoreactive proteins were visualized using the LumiGLO chemiluminescent substrate (Cell Signaling).
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bioRxiv - Genetics 2024Quote: Mouse embryonic stem cells (ESCs) were grown on 0.1% gelatin-coated culture dishes in Glasgow Minimum Essential Medium (Sigma) supplemented with 20% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... One hundred microliters of the E16.5 mouse brain lysate were digested with 1 unit of hyaluronidase from Streptomyces hyalurolyticus (Sigma) at 37L for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... with two PBS washes after each incubation: primary antibody solution for 1 hour (Mouse Anti-phospho-Histone H2A.X Ser139 Antibody, clone JBW301, Sigma #05-636 ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 12 hr at 4°C in the presence of mouse anti-FLAG (1:1000, Sigma #F1804) and rabbit anti-CD40 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... The blocking solution was then replaced with another consisting of 2 µL mouse anti-NeuN antibody (Merck Millipore, MAB377) and 0.5 µL of rabbit anti-GFAP antibody (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... we use primary anti-acetylated tubulin antibody from mouse (monoclonal 6-11 B-1, T7451 Sigma, 1/1000 dilution) as well as primary anti-tyrosinated tubulin antibody from mouse (monoclonal TUB-1A2 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated in a solution containing mouse monoclonal anti-BrdU antibody (1:250, Sigma, Cat.no. B8434) and rabbit polyclonal anti-Doublecortin (DCX ...
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bioRxiv - Biochemistry 2024Quote: ... anti-p-Histone H2A.X Ser139 (ψH2A.X, mouse monoclonal, 05-636) and pan-ADP-ribose binding reagent (rabbit Fc tag, MABE1016) were obtained from Millipore. anti-DHFR (rabbit polyclonal ...
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bioRxiv - Biochemistry 2024Quote: His6-tagged CCT domains (mouse SPAK 459-556 and human OSR1 433-527) and mouse SPAK 62-556 T243E were expressed in Rosetta (DE3) E.coli (Novagen). Cells were grown at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The following primary antibodies were used: GFP (rabbit, in-house, 1:1,000) and Tubulin (mouse monoclonal, 1:8,000; Sigma-Aldrich). As secondary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were tested on the Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore, Cat No. MCYTOMAG-70K-PMX) according to the kit protocol as qualified ...
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bioRxiv - Cancer Biology 2024Quote: The following primary antibodies and dilutions were used: mouse-anti-γH2AXSer139 1:1,000 (#05-636, clone JBW301, Merck Millipore) and rabbit-anti-53BP1 1:1,000 (NB#100-304 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 x in TBS-Tween (0.1%) + 2% BSA before incubation in TRITC conjugated anti-Mouse secondary antibody (Sigma) at 1:1000 concentration for 1 hr in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 48 h at 4°C with either a mouse anti-α-tubulin (DM1A; Sigma-Aldrich) primary antibody at 1:5000 in PBT ...
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bioRxiv - Cell Biology 2024Quote: ... Incubation with primary mouse monoclonal anti-TFR1 antibody (clone 3B82A1, catalog# MABS1982, lot# 3519825, EMD Millipore, Burlington, MA, USA) diluted 1:250 with 0.2% BSA ...
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bioRxiv - Developmental Biology 2024Quote: ... then incubated for 1 hr with an goat anti-mouse HRP-antibody (EMD Millipore, #12-349, diluted 1:10,000). After three more washes with HRP wash ...
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bioRxiv - Cell Biology 2019Quote: ... HEK-293T cells were cultured in suitable medium and transfected by calcium phosphate method with the following plasmids: 10 μg of pLKO.1 (Sigma-Aldrich, St Louis, MO, USA – SHCLNG-NM_000259), containing the shRNA cassette ...
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bioRxiv - Plant Biology 2022Quote: ... were transfected with the purified plasmids (20–30 μg) by adding an equal volume of PEG-Ca solution (40% PEG4000 [Sigma-Aldrich], 0.2 M mannitol, 100 mM CaCl2) for 5–6 min ...
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bioRxiv - Plant Biology 2021Quote: ... We prepared InsP7 by using 100ng of Mouse IP6K1 (mIP6K1) recombinant protein along with 100 µM of InsP6 (Sigma, USA) in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were isolated from a single mouse hemisphere in 2 ml of pre-chilled EZ PREP lysis buffer (NUC-101, Sigma) using a glass Dounce tissue grinder (D8938 ...
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bioRxiv - Microbiology 2021Quote: Serum concentrations of Metabolic Panel (Ghrelin, Glucagon, Insulin and Leptin) were determined using the Mouse Metabolic Magnetic Bead Panel Milliplex MAP kit (Millipore).
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bioRxiv - Microbiology 2021Quote: ... IgE) and IgA in feces supernatant were determined using the Mouse Immunoglobulin Isotyping Magnetic Bead Panel Milliplex MAP kit (Millipore) and Mouse IgE Single Plex Magnetic Bead Milliplex MAP kit (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were stained with primary (1:1000, guinea pig anti-NeuN, Millipore #ABN90P; 1:1000, mouse anti-NeuN, Millipore #MAB377) and secondary antibodies (1:400 ...
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bioRxiv - Systems Biology 2020Quote: ... mouse and naked mole rat fibroblasts were cultured as described earlier and treated with varying concentrations of AZC (Sigma-Aldrich). After 5 days of treatment ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared by centrifugation at 8000 g for 10 min and pre-cleared by pre-incubated with 100 μl Mouse IgG−Agarose beads (A0919 Sigma) without antibody for 2 h at 4°C on wheel ...