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Citations for Millipore Sigma :
9551 - 9600 of 10000+ citations for Mouse FAM117A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... and eluates were separated by SDS-PAGE and analyzed by Western blotting using mouse α-FLAG M2 (Sigma #F1804).
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bioRxiv - Neuroscience 2020Quote: Whole-cell lysates were prepared from whole mouse brains using CST buffer (Cell Signal Technology) containing protease inhibitor (Sigma), and were rotated at 4°C for 30 minutes before DNA was pelleted ...
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bioRxiv - Immunology 2019Quote: ... Horseradish peroxidase (HRP)-linked goat anti-rabbit and goat anti-mouse IgG secondary antibodies were purchased from Sigma-Aldrich.
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bioRxiv - Immunology 2019Quote: ... resuspended in RPMI-10% FBS containing 40ng/mL CTLA4-Ig (CD152/Fc chimera, non-cytolytic, from mouse, Sigma C4358), plated in 24-well culture plates at a density of 2 x 106 cells/well and cultured for an additional 24 hours (BMDCCTLA4-Ig) ...
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bioRxiv - Biochemistry 2020Quote: ... to detect the nanobodies and secondary antibody anti-mouse coupled to horse radish peroxidase (HRP) (0.5 μg/ml – Millipore). 50 μl of 1-Step UltraTMB-ELISA (ThermoScientific ...
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bioRxiv - Microbiology 2019Quote: ... overnight at 4°C at a 1:1000 at dilution and α-mouse IgG (whole molecule)-peroxidase (Sigma #9044) for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... After incubation at room temperate for 2 hr with a 1:5,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Sigma), foci were detected by addition of TrueBlue substrate (KPL) ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times for 5 min and incubated with anti-mouse-HRP antibody (0.2 μg/ml – Millipore) for 1 h in TBST ...
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bioRxiv - Immunology 2019Quote: ... mouse peritoneal neutrophils were harvested 4 h after intraperitoneal injection of 2 ml of 4% thioglycollate broth (Sigma-Aldrich). The collected peritoneal neutrophils were cultured in RPMI 1640 (Welgene ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with primary antibodies diluted in 1% NDS-PBST (1:2,000 for mouse anti-GFAP, Sigma; 1:1,000 for chicken anti-GFAP ...
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bioRxiv - Microbiology 2019Quote: ... injection with 200 μL per mouse of a 1:1 (v/v) mixture of Freund’s complete adjuvant (Sigma-Aldrich) and a solution containing 250 μg of purified rTMPT2A ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated overnight at 4°C in mouse anti-NeuN primary antibody (#MAB377, RRID:AB_2298772, Millipore Sigma, Billerica, MA) at a 1:1000 concentration in diluted blocking solution (10% fetal plus ...
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bioRxiv - Evolutionary Biology 2021Quote: Pulldown experiments assayed by western blotting were visualized using a commercially available mixture of Mouse ⍺-GFP clones 7.1 and 13.1 (Sigma-Aldrich) at a dilution of 1:103 for the primary antibody incubation followed by secondary incubation with goat ⍺-mouse conjugated to horseradish peroxidase (1:104 dilution ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extraction from skelatal muscle tissue or C2C12 mouse myoblasts was done using TRI Reagent (Millipore-Sigma #T9424). Muscle tissue samples were flash-frozen in liquid nitrogen until further processing ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extraction from skelatal muscle tissue or C2C12 mouse myoblasts was done using TRI Reagent (Millipore-Sigma #T9424). Muscle tissue samples were flash-frozen in liquid nitrogen until further processing ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5 μl blocking buffer (10 mg/ml mouse IgG with 5 mM Azide) and 0.5 μl 0.5% Phenol red (Sigma P0290) were added for blocking the unconjugated Anti-Mouse-IgG-Atto647N and vortexed thoroughly ...
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bioRxiv - Molecular Biology 2020Quote: ... blocked with 5% non-fat dry milk in TBS-0.1% Tween-20 for 1 h and incubated overnight with mouse anti-ataxin-3 (1:1000; Millipore), mouse anti-HA (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... the slides were incubated in primary antibody (Mouse anti-TH antibody, 1:1000, product #MAB5280, Millipore, Billerica, MA, USA) for 1 hour at room temperature and 36 hours at 4° C ...
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bioRxiv - Neuroscience 2020Quote: ... or AAVretro-EF1a-Cre primary antiserum (1:100 mouse-anti-Cre Recombinase antibody, clone 2D8, Millipore Sigma Cat# MAB3120) mixed with donkey serum ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... The following primary antibodies were used for immunoblotting at the indicated dilutions: mouse monoclonal anti-FLAG (Sigma-Aldrich, F1804), 1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained for intracellular markers with anti-α-SMA (mouse IgG2a, Sigma Aldrich, St. Louis, MO, 1/250), anti-desmin (rabbit polyclonal IgG ...
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bioRxiv - Microbiology 2022Quote: ... Immunized mice sera followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Antibody complexes were detected using the ImmobilonTM Western systems (Millipore).
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibody staining for Dlg1 (1:50; mouse, Developmental Studies Hybridoma Bank) and GFP (1:500; rabbit, EMD Millipore) was done in PBT (PBS/0.1% BSA/0.1% Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed 3 times in PBS and incubated at room temperature for 30 min with a goat anti-mouse Ig polyclonal secondary antibody conjugated with alkaline phosphatase (0.5 µg/mL, Sigma). After 3 washes with PBS ...
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bioRxiv - Immunology 2020Quote: ... for 1 hour at RT and detection of anti-mouse IgG by the addition of pNPP substrate (Sigma-Aldrich). An arbitrary number of ELISA units were assigned to the reference pool and OD values of each dilution were fitted to a 4-parameter logistic curve using SOFTmax PRO software ...
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bioRxiv - Developmental Biology 2021Quote: ... 293T or AL1 cells were incubated for 3 hours with anti-Myc mouse monoclonal antibody (Sigma-Aldrich; 1:500), and secondary staining was performed using anti-mouse AlexaFluor568 antibodies (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... followed by the incubation with the primary antibodies: mouse anti-GAD 67 primary antibody (Millipore, Mab5406, 1:1000 dilution), and rabbit anti-GFP primary antibody (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: E14 mouse embryonic stem cells (ESCs) were grown in two different media: ‘serum+LIF’ consisted in Glasgow medium (Sigma), 15% FBS (Gibco) ...
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bioRxiv - Cell Biology 2019Quote: Mouse anti-α-tubulin (DM1A; 1:1000 for indirect immunofluorescence staining [IF]; Cat#T9026, Sigma-Aldrich, St. Louis, MO), rat monoclonal anti-α-tubulin (YL1/2 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with 3% milk in PBS + 0.05% Tween-80 (PBST) and then incubated overnight with primary antibodies (monoclonal mouse anti-FLAG [to detect MurJ; Sigma] ...
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bioRxiv - Neuroscience 2021Quote: ... was injected at P7 and/or P8 for the GdnfCreER line at 0.1 mg/g mouse using 10 mg/mL Tamoxifen dissolved in sunflower oil (Sigma) with 10% ethanol ...
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bioRxiv - Immunology 2021Quote: ... The bound antibodies were revealed using a HRP-conjugated goat antibody to mouse Ig absorbed with human Ig (Sigma) (1:4,000 dilution) ...
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bioRxiv - Microbiology 2021Quote: ... After incubation at room temperature for 2 hr with a 1:5,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Sigma), foci were detected by addition of TrueBlue substrate (KPL) ...
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bioRxiv - Immunology 2020Quote: ... The plates were incubated sequentially with 500 ng/mL previously described mouse anti-ZIKV mAb ZV-165 and horseradish-peroxidase (HRP)-conjugated goat anti-mouse IgG in PBS supplemented with 0.1% (w/v) saponin (Sigma) and 0.1% bovine serum albumin (BSA) ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-mouse IgG HRP antibody (AP192P, Merck Millipore). Protein detection was performed using SuperSignal West Pico PLUS (34579 ...
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... spirochetes were incubated for 60 min with goat anti-mouse gold-labeled antibody at 1:30 dilution (G7652; Sigma). The grid was further washed ...
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bioRxiv - Cell Biology 2020Quote: ... Subsequently the PLA protocol was followed according to the Duolink In Situ Red Starter Kit Mouse/Rabbit (Millipore Sigma) instructions ...
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bioRxiv - Cell Biology 2021Quote: ... rat anti-mouse RIPK3 (WEHI clone 8G7; produced in-house7 and soon available from Millipore as MABC1595; 1:2000); rabbit anti-phospho-T231/S232 mouse RIPK3 (Genentech ...
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bioRxiv - Immunology 2021Quote: ... Anti-rabbit IgG-HRP (Cat no.- A0545) and Anti-mouse IgG-HRP (Cat no.- A9044) were purchased from Sigma. Anti-mouse IL-10 ELISA set (Cat no ...
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bioRxiv - Cell Biology 2021Quote: ... the following primary antibodies were used at the indicated dilutions: mouse anti-γ-tubulin 1:500 (T5326; Sigma Aldrich), rabbit anti-mCherry 1:500 (ab167453 ...
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bioRxiv - Genetics 2019Quote: ... The following primary antibodies were used: mouse anti-acetylated tubulin (clone 6-11-B1, Sigma-Aldrich, T6793; 1:1,000), mouse-anti beta-actin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... while mouse primary astrocytes (~3.5 × 105 cells) were seeded on poly-L-lysine coated transwell filters (3450; Sigma-Aldrich) and maintained in astrocyte-media ...
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bioRxiv - Cell Biology 2021Quote: ... the oocytes were washed three times and incubated with FITC-labelled mouse anti-α-tubulin (1:200, Sigma-Aldrich) for 1 h at 37°C.
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bioRxiv - Genetics 2020Quote: ... Proteins were detected using either secondary anti-mouse or anti-rabbit IgG coupled to peroxidase (Millipore, 1:5000 dilution), western bright ECL substrate (Advansta ...
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bioRxiv - Genetics 2020Quote: ... blots were incubated with the following antibodies: monoclonal mouse anti-GFP (Sigma, MO; clone 7.1 and 13.1, 1:1000), mouse anti-Tubulin (DHSB ...
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bioRxiv - Microbiology 2022Quote: Plastic tissue culture coverslips were coated with normal mouse serum (50% diluted in DPBS with CaCl2 and MgCl2) (Sigma) for 1 hr at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... On selected sections, immunoreactivities were tested: SATB1 (mouse, 1:500, SantaCruz) and GluR 2/3 (rabbit, 1:500, Millipore) were diluted in 0.1 M PB and incubated overnight at room temperature ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Binding of cGAS and dsDNA in human and mouse brain sections was determined by PLA kit (DUO92101, Sigma-Aldrich) accordingly to the manufacture’s instruction ...