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Citations for Millipore Sigma :
9951 - 10000 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The whole slide was then warmed for 1 min at 37°C and immediately fixed for 10 min at room temperature (RT) in a 2% formaldehyde solution (1:20 37% formaldehyde acquired from Sigma-Aldrich, Missouri, USA in 1x PBS pH 7.4). The sections were dried with isopropanol and stained with hematoxylin and eosin (H&E) ...
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bioRxiv - Neuroscience 2020Quote: Genetic recombination in GLAST-CreERT2;R26R-EYFP animals was induced by a daily intraperitoneal injection of 2 mg of tamoxifen (Sigma, 20 mg/ml in 1:9 ethanol:corn oil) for five consecutive days ...
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bioRxiv - Zoology 2023Quote: ... Each egg-sperm bundle was placed in a 2 mL microcentrifuge tube and allowed to break up in 0.1 mL of seawater and for the eggs to hydrate for 2 hrs before preserved in zinc fixative (1:4 Z-fix, Sigma-Aldrich Inc. to 0.2 µm filtered seawater FSW). Preserved eggs from each bundle were photographed using an Olympus SZX7 dissecting microscope equipped with an Olympus America camera (SN ...
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bioRxiv - Microbiology 2022Quote: ... Glucose kit: Glucose (HK) Assay Kit (Sigma, product Code GAHK-20). Citric acid kit ...
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bioRxiv - Cell Biology 2020Quote: ... and adapted to 2i condition by growing them in gelatin-coated flasks in N2B27 medium (50% neurobasal medium [Gibco], 50% DMEM/F12 [Gibco], 2 mM L-glutamine [Gibco], 0.1 mM β-mercaptoethanol, NDiff Neuro-2 supplement [Millipore], B27 serum-free supplement [Gibco]) supplemented with 1,000 units/mL LIF ...
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bioRxiv - Neuroscience 2019Quote: ... Mice were anesthetized with an intraperitoneal injection of 250 mg/kg Avertin (mixture of 2,2,2-tribromoethyl alcohol 2%, Sigma-Aldrich, and tert-amyl-alcohol 2%, Merck), except for the Mbpneo/neo line ...
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bioRxiv - Pathology 2020Quote: Ela-Cre AT-1fl/+ and Ela-Cre AT-1fl/fl mice at 2-3 months of age were treated with tamoxifen (MP Biomedicals, 3mg/40g BW dissolved in 98% corn oil [Sigma] and 2% ethanol [Sigma]) by oral gavage once daily for three days to induce Cre recombinase activity ...
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bioRxiv - Microbiology 2019Quote: ... A 200 μL aliquot of a solution containing 100 μg/μL of XTT (2, 3-(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) (Sigma-Aldrich, St Louis, MO, USA) and 10 μg/μL of PMS (phenazine methosulfate ...
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bioRxiv - Immunology 2020Quote: ... and primary antibodies in the presence of Fc block in flow buffer (DPBS [Gibco] supplemented with 2% FCS, 2 mM EDTA [both Sigma] and 0.01% sodium azide [Morphisto]). After staining with secondary biotin-coupled antibodies in flow buffer ...
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bioRxiv - Immunology 2020Quote: ... TZM-bl cells infected with JR-FL without Env (No Env virus) were treated with 100 mM 2-deoxy-glucose (2-DG) (Sigma-Aldrich, St. Louis, MO, USA) 2 h prior to image acquisition.
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bioRxiv - Neuroscience 2022Quote: ... subcutaneous injection) then demyelinating lesions were induced by stereotaxically injecting 2 μl of 2 μg lysolecithin (LPC; egg yolk, Sigma Aldrich Cat. No. L4129-100MG) diluted in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... inhibitor MG-132 (474790), 2-deoxy-D-glucose (2-DG, D6134), and palmitic acid (C16:0, P9767) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... The 1.5 ml resuspended RBCs were cultured in 50 ml HPS containing 2 mM CaCl2 and 2 µM calcium ionophore-4-bromo-A23187 (C7522, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) in T75 flasks in a 5% CO2 incubator at 37°C for 16 h ...
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bioRxiv - Neuroscience 2023Quote: ... The pocket was filled with conductive gel (SuperVisc, EasyCap GmBH, Germany) and a 2 by 2 mm platinum stimulation electrode (#349356-600MG, Sigma-Aldrich, Inc., St. Louis, MO) was inserted inside ...
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bioRxiv - Bioengineering 2020Quote: ... 8 μL of 10% methacrylated-chondroitin-sulfate-A-glycosaminoglycan; 7.58 μL neurobasal media; 4 μL of 1% photocrosslinker Irgacure-2959, Sigma-Aldrich; 1.21 μL of FGF-2; 1.21 μL of BDNF), loaded in a 50 μL Hamilton Syringe (fitted blunted 21 Gage tight lock stainless steel needle ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 μL of each lysate (concentrations adjusted to 2.5 μg/μL) was treated with 6 μL of premixed click chemistry mixture (final concentrations of 100 μM biotin-N3 (Sigma-Aldrich, 762024), 1 mM CuSO4 ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
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bioRxiv - Developmental Biology 2021Quote: Recombination of the conditional allele was induced in Atxn10flox/flox;CAGG-creERT2 mice at 6 and 8 weeks old by a single intraperitoneal (IP) injection of 9mg tamoxifen (Millipore Sigma, T5648) per 40g (body weight ...
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bioRxiv - Molecular Biology 2021Quote: ... A BioSpec bead-beater was used to lyse cell pellets corresponding to 3-6 OD600 units in the presence of 100 μL of acid washed beads and 300 μL of 20 % TCA (Sigma-Aldrich T6399). 2 x 3-minute pulses were used ...
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bioRxiv - Biochemistry 2019Quote: apoTaLPMO9A was prepared by dialyzing the purified TaLPMO9A extensively using D-tube Dialyzer Maxi (6-8 kDa) from Millipore (Billerica, MA, USA). Dialysis was performed in 20 mM phosphate buffer (pH 7 ...
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bioRxiv - Immunology 2019Quote: ... erythrocytes were depleted by sequentially layering 10 ml PBS followed by 8 ml of 6% w/v dextran (high molecular weight, Sigma-Aldrich, in PBS) and gently inverting ...
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bioRxiv - Neuroscience 2020Quote: ... and MT+SNL mice from tissues collected on day 6 (post behavioral testing) using Tri Reagent (T9424, Sigma Aldrich, St. Louis, MO) and RNeasy Micro kit (74106 ...
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bioRxiv - Neuroscience 2021Quote: ... iPSC-derived neuron progenitors were expanded using 6-well plates coated with 15 μg/ml Poly-L-Ornithine (Millipore Sigma Cat# P4957) and 10 μg/ml laminin (Invitrogen Cat# 230171015 ...
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bioRxiv - Neuroscience 2020Quote: Primary hippocampal neurons were isolated from P1 C57BL/6 mice by dissociating with 0.25% trypsin and plated onto poly-L-lysine (Sigma-Aldrich, MO, USA)-coated culture dish ...
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bioRxiv - Neuroscience 2020Quote: ... The heads were placed in a 15 ml centrifuge tube filled with perfluoropolyether (Fomblin Y, LVAC 16/6, average molecular weight 2700, Sigma-Aldrich, U.S.A.). Data was acquired on a small animal MRI system (BioSpec 94/30 animal MRI system ...
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bioRxiv - Immunology 2021Quote: ... Fab samples were concentrated to ~6-8 mg/ml and buffer exchanged to TBS using Amicon ultrafiltration units with a 10 kDa cutoff (EMD Millipore Sigma). Final Fab yields were ~1 mg ...
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bioRxiv - Neuroscience 2020Quote: Different cellular and mitochondrial parameters of the glutamate- or erastin-induced cell death pathways were analyzed using the Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany) upon harvesting adherent HT22 cells and following addition of different fluorescent dyes.
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bioRxiv - Neuroscience 2020Quote: Focal white matter demyelinating lesions were induced in the cerebral striatum of 12-week-old male C57BL/6 mice by stereotaxic injection of 4 μL of 0.01% (v/v) ethidium bromide (Sigma-Aldrich, Shanghai, China) using a Hamilton syringe ...
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bioRxiv - Cancer Biology 2022Quote: HEK293T cells at 80-90% confluency in a 12-well plate were transfected using 6 μL GeneJuice Transfection Reagent (Sigma-Aldrich, 70967) and 100 μL RPMI 1640 medium (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... single cell suspensions were derived from bone marrows of 4-6-week-old C57BL/6 WT and Igh VH locus inversion mice and incubated in Red Blood Cell Lysing Buffer (Sigma-Aldrich, #R7757) to deplete the erythrocytes ...
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bioRxiv - Biochemistry 2021Quote: ... Female C57BL/6 mice wild type mice (7-9 weeks) were injected intraperitoneally with etomoxir (100 µg, Sigma Aldrich, Cat. No E1905) and/or LPS (1 µg ...
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bioRxiv - Molecular Biology 2020Quote: Confluent monolayers of HAEC grown on 6-well plate were exposed to unidirectional PSS (6, 12 and 24 hours) using a modified flow device.10 Neutralized MCDB-131 medium (Sigma Aldrich, MO) containing 7.5% sodium bicarbonate solution ...
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bioRxiv - Neuroscience 2021Quote: ... 6; MgCl2, 4; CaCl2, 5; sucrose, 160; glucose, 25; Hepes, 10; pH 6.7, 500 mOsmol; all chemicals from Sigma-Aldrich, Lyon, France), to avoid desiccation of the brain surface ...
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bioRxiv - Neuroscience 2022Quote: ... mice aged 5 – 6 weeks and harboring the Fezf2-CreER allele were treated with 50 mg/kg body weight tamoxifen (Sigma-Aldrich,T5648) by oral gavage for 5 consecutive days ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded in 6-well plates the previous day and treated either with 200ug/ml Cycloheximide (CHX) (Sigma-Aldrich # C-7698) in fresh growth media for 2 ...
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bioRxiv - Immunology 2022Quote: ... RPMI-1640 media containing 10% FBS was supplemented with antibiotics (6 mg/l Nitrofurantoin, Sigma Aldrich, cat. no. N7878; 16 mg/l Imipenem monohydrate, Sigma Aldrich, cat ...
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bioRxiv - Zoology 2019Quote: The midgut tissue dissected out from continuous toxin-exposed susceptible and toxin exposed insects for 6 generations (n=5) were processed for RNA isolation using TRI reagent TM (Sigma Aldrich, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Then ten half seeds with involved embryos were placed in a Petri dish containing 6 ml of distilled water with or without (±) 10 μM of ABA (Sigma Chemical Co.). The Petri dishes were then incubated in the dark at 20°C ...
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bioRxiv - Pathology 2021Quote: ... Infected tissues were disrupted and digested overnight (12 hours) using Fast Prep® Lysing Matrix tubes containing 0.2 mg (6 U) Proteinase K (Sigma-Aldrich®) diluted 1/40 in Lifton’s Buffer (100 mM EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... were pooled together (6 mL) and concentrated/buffer exchanged using an Amicon® Ultra-4 Centrifugal Filter Units (3 kDa, Millipore, Ireland). The final volume of the purified bacteriocin protein fraction was reduced to 200 μL ...
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bioRxiv - Neuroscience 2019Quote: ... filled with a thin layer of 2.5% agarose containing either pure agarose (Sigma Aldrich, cat. no.: A5093, CAS no.: 9012-36-6) or agarose plus 1.5 M sodium chloride (Sigma Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... The heads were then placed in a 15 ml centrifuge tube filled with perfluoropolyether (Fomblin Y, LVAC 16/6, average molecular weight 2700, Sigma-Aldrich, U.S.A.). Samples were measured at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... isolated of at least 6 animals per preparation were mixed with collagen type I (PureCol™ EZ Gel solution, # 5074, Sigma-Aldrich, Vienna ...
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bioRxiv - Neuroscience 2021Quote: ... Y1eGFP mice at 6-8 weeks of age were transcardially perfused with chilled phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (Sigma-Aldrich, USA) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were seeded in 6-well plates and 24 h later CIGB-300-F and Hoechst 33342 (Sigma, St. Louis, MO, USA) were added at a final concentration of 30 μM and 4 μg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... Lentiviral-containing supernatant was collected at 72 hours and used to transduce SAF-A-AID-mCherry cells in the presence of RPMI media supplemented with 10% FBS and 6 μg/mL polybrene (Sigma, H-9268). After 48 hours ...
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bioRxiv - Developmental Biology 2021Quote: The proteins in the sample were precipitated using methanol/chloroform and were resuspended in 6 M Guanidine HCl (Sigma cat# G3272-1KG), buffered using 50 mM EPPS (Alfa Aesar cat# A13714) ...
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bioRxiv - Biophysics 2021Quote: ... 3C cleavage was performed in 30 mins and the protease was separated from HsPepT2 by gel filtration using a Superose® 6 Increase 10/300 (Sigma-Aldrich). The top fraction was concentrated to 10 mg/mL using a 100 kDa cutoff concentrator (Corning Spin-X UF concentrators ...
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bioRxiv - Molecular Biology 2021Quote: ... then they were brought back to room temperature and then they were alkylated using 6 µmoles of iodoacetamide (BioUltra; Sigma-Aldrich, USA) in the dark at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: Water samples were filtered within 6 hours of sampling under gentle vacuum (∼380 mm Hg) on 0.45-µm polycarbonate filters (Millipore Isopore, Billerica, MA). Filters were immediately placed into CTAB buffer (100 mM Tris-HCl (pH 8) ...