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Citations for Millipore Sigma :
9801 - 9850 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 300 μl condensed lentivirus was added to 6 ml ES media with 48 μg polybrene (EMD Millipore Corp, TR-1002-G). After 6 hours of incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... each NBC population was thawed into a 6-well plate and infected with a unique barcode-containing lentivirus supernatant in medium with Polybrene (Sigma Aldrich) at a concentration range from 1:200 to 1:50 ...
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bioRxiv - Cancer Biology 2019Quote: Cells with a density of 40-60 % (2D) or 6 days after seeding onto Matrigel (3D) were treated with 40 ng/ml colcemid (Sigma-Aldrich, St ...
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bioRxiv - Cancer Biology 2019Quote: HBP inhibition in vivo was achieved by treatment of tumor bearing mice with 3 doses (10 mg/kg) of DON (6-Diazo-5-oxo-L-norleucine, Sigma-Aldrich) every 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... Finally the desired protein was eluted form the beads by incubation with 100 μl of Elution Buffer containing 6 mg/ml of GSH (Sigma, 4251) during 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Transverse sections (6 µm) were sectioned and mounted on silane-covered (TESPA, 3-aminopropyl-triethoxysilane, Sigma-Aldrich, St. Louis, Missouri, USA) glass slides ...
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bioRxiv - Microbiology 2020Quote: ... and fractions containing target protein were collected and concentrated to 6-8 mg/ml using Amicon concentrators (Millipore, Billerica, MA, USA), flash-frozen with liquid nitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Human melanocytes seeded at 1.0×105 – 2.0×105 cells/well density in 6-well plates were incubated in viral supernatant with 10µg/mL polybrene (Sigma, TR-1003) and centrifuged at 300xg for 60 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Mitochondria were incubated with 0.2 μM TMRE for 15 minutes and then measured with Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany)
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bioRxiv - Neuroscience 2020Quote: ... was used as a positive control and incubated simultaneously with MitoSOX Red indicator at a concentration of 1.25 μM and afterwards measured with Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany)
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bioRxiv - Pathology 2021Quote: PLA was performed in cryostat sections (4–6 μm) obtained from frozen samples of human parietal cortex using the Duolink In Situ Detection Reagents Brightfield system (Sigma-Aldrich). This method monitors the coincidence of specific epitopes and has been previously used for monitoring protein phosphorylation (77) ...
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bioRxiv - Molecular Biology 2021Quote: ... VICs were seeded at 1×105 cells/well into 6-well culture plates and stimulated with FeSO4 (10 μmol/L) concentrated with iron(II) sulfate heptahydrate (Sigma, USA) in PBS ...
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bioRxiv - Microbiology 2019Quote: The Nafion films on the IDAs mounted in the Teflon cell were loaded with various amounts of Ru(NH3)6 3+ by either exposing the films to a 10 mM solution of Ru(NH3)6Cl3 (Sigma Aldrich) in 0.2M Na2SO4 at time points from several minutes to several hours or by allowing Ru(NH3)6 3+ in the films to diffuse out into bulk electrolyte for several hours ...
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bioRxiv - Cancer Biology 2021Quote: ... A549 cells were then infected by culture-medium-diluted viral supernatants in the presence of 6 μg/ml polybrene (Sigma #H9268) in 3 consecutive days and selected with puromycin (1.5 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... collagen-grown cells were collected and incubated with ITGA-6 neutralizing antibody (Merck Millipore #MAB1378, clone NKI-GoH3 at 40ug/ml) for 60 mins at 37 C before plating on hydrogels ...
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bioRxiv - Molecular Biology 2022Quote: ... dissociated monolayer cells were seeded inside a 50uL Matrigel droplet on a 6-well clear polystyrene flat-bottom plate (Millipore Sigma) at a density of 250 cell/uL Matrigel in the indicated media conditions + 1uM 4OHT or an equivalent volume of DMSO at the time of seeding ...
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bioRxiv - Microbiology 2022Quote: ... For verification of the expected product size and RNA integrity, RNA gels (6 % PAA, 7 M urea) were prepared with subsequent staining using StainsAll (Sigma-Aldrich).
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bioRxiv - Bioengineering 2022Quote: ... the cells were supplemented with 500 µL antibiotic-free media and cultured for up to 6 days in RPMI 1640 media (Sigma-Aldrich). At each time point ...
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bioRxiv - Cell Biology 2022Quote: ... in medium and incubation for 6 h prior cell lysis or the supplementation of pre-warmed medium with 10 mM DTT (Sigma-Aldrich) for one hour ...
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bioRxiv - Immunology 2020Quote: ... Sorted cells were incubated with slices (5×105 cells/slice) for 6 hours in Hanks’ Balanced Salt solution (Sigma-Aldrich, USA) supplemented with 1% of penicillin/streptomycin solution (Thermo-Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... ciprofloxacin hydrochloride monohydrate (stock solutions prepared in DI water) and amoxicillin (stock solution prepared in phosphate buffer, pH 6) were purchased from Sigma-Aldrich. Solutions of antibiotics in media were prepared shortly before the experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... crystals were rapidly passed through a drop of fluorinated oil (Fomblin® Y LVAC 14/6, average MW 2,500 from Sigma Aldrich) to prevent ice formation and directly frozen on the beamline in the nitrogen stream at 100 K ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were sonicated for two times ten secs at 50% amplitude on ice and incubated for 30 min at RT in lysis buffer consisting of 6 M urea (Sigma-Aldrich), 2 M thiourea (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were passaged after reaching 80% confluency by washing with PBS (Dulbecco’s Phosphate Buffered Saline, Sigma-Aldrich, Cat. #D8537-6×500ML) and incubating in 1 X trypsin-EDTA (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were plated on polyester membrane inserts (0.4 μm pore) in 6 well culture plates (Corning Costar CLS3450-24EA, Sigma-Aldrich, UK), 2-3 slices per insert ...
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bioRxiv - Neuroscience 2022Quote: ... The permeability of the cupular matrix was measured by recording the penetration of a negatively-charged fluorophore (6-carboxyfluorescein; Sigma-Aldrich) into the cupula ...
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bioRxiv - Cell Biology 2022Quote: ... trypsinized cells were resuspended in standard growth medium without antibiotics and plated in 6-well culture plates (5 × 104 cells/well) after mixing with 10 µg/ml polybrene (EMD Millipore) and lentivirus supernatant ...
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bioRxiv - Genetics 2022Quote: 750k low-passage 293FT cells were seeded into each well of a 6-well plate coated with poly-L-ornithine (Sigma, P2533). One day after seeding ...
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bioRxiv - Neuroscience 2022Quote: ... CAS# 614-96-0), 6-methylindole (97%, CAS# 3420-02-8) and 7-methylindole (97%, CAS# 933-67-5) were acquired from Sigma-Aldrich, and 4-methylindole (99% ...
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bioRxiv - Developmental Biology 2019Quote: ... and homogenized in ATP assay Lysis buffer (6 M Guanidine-HCl, 100 mM Tris pH 8 and 4 mM EDTA, all from Sigma Aldrich, Cat# G3272 ...
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bioRxiv - Physiology 2019Quote: ... Cells were then incubated with activator Phorbol ester 12-O-tetradecanoylphorbol-13-acetate (PMA; Sigma, Schnelldorf, Germany; 0.1 μM; 6 h) with or without 1 μM PKC inhibitors Calphostin C (Tocris) ...
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bioRxiv - Cell Biology 2019Quote: Confluent HeLa cells grown in 6- or 12-well plates were treated with deferiprone (DFP; 379409, Sigma-Aldrich; working concentration, 1mM) for the time durations as indicated.
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bioRxiv - Cell Biology 2019Quote: ... were added to each well (around 6 × 105 cells per well) along with a no virus control with 8 µg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight (12-16 hours) ...
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bioRxiv - Immunology 2020Quote: ... miR-511-3p transfected cells as well as controls were stimulated on day 6 with RSG in the presence or absence of LPS (Sigma Aldrich) for 24 hours before harvesting ...
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bioRxiv - Molecular Biology 2020Quote: For lentiviral transduction 200.000-250.000 ES cells per well were seeded on gelatinized 6-well dishes in ES medium containing 8 μg/ml polybrene (Sigma, #H9268). Viral supernatant was added to the cells and plates were spun in a prewarmed centrifuge (Heraeus Multifuge 4KR ...
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bioRxiv - Immunology 2020Quote: ... Membranes were then thoroughly washed (6 × 10 min) with PBS-T before revealed with SigmaFast NBT/BCIP substrate solution (Sigma-Aldrich).
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bioRxiv - Plant Biology 2020Quote: ... 5 μl PCR product for each barcoded sample was mixed with 5 μl 6×Orange G DNA Loading Dye (Orange G, 6X, Sigma, Germany) and run on a 1 % agarose gel in TAE 1X buffer ...
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bioRxiv - Genomics 2020Quote: ... transfected in the Lonza 4D nucleofector program ‘CA-137’and seeded onto a Matrigel-covered 6-well plate in 3 ml StemFlex containing 10 μM Thiazovivin (Millipore, #S1459). The cells were subjected to a 48 hour cold shock at 32 °C to enhance homology directed repair (HDR) ...
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bioRxiv - Bioengineering 2020Quote: ... However initial mineralized collagen suspensions were created using one of three potential glycosaminoglycans: chondroitin-6-sulfate (Chondroitin sulfate sodium salt from shark cartilage, Sigma-Aldrich), chondroitin-4-sulfate (Sodium Chondroitin Sulfate A ...
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bioRxiv - Synthetic Biology 2020Quote: ... lentivirus was added to cells at a multiplicity of infection of 10–50 in the presence of 6 mg/ml of DEAE-dextran (Sigma Aldrich). After 18 hours ...
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bioRxiv - Bioengineering 2019Quote: ... HUVECs (12×106 cells/mL) and MSCs (6×105 cells/mL) were suspended in EBM-2MV supplemented with thrombin (4 U/mL) (Sigma-Aldrich). A solution of fibrinogen (8 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... the acid insoluble fraction was incubated for 18 hours at 4°C with 500 μL of 6 M guanidine hydrochloride/100mM TRIS buffer (pH 7.4, Sigma-Aldrich, U.K.). The buffer was exchanged into 100 μL of 50 mM ammonium acetate (Scientific Laboratory Supplies ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were habituated for 6 days to our animal facility before starting treatment with p-Cresol (reference W233706-SAMPLE-K, Sigma-Aldrich) dispensed in sterile drinking water at a concentration of 0.25 g/L ...
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bioRxiv - Molecular Biology 2021Quote: FSHD immortalized myoblast cells were seeded in 6-well plates pre-coated with extracellular matrix gel from Engelbreth-Holm-Swarm murine sarcoma (Sigma, UK). Following transfection with PMOs ...
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bioRxiv - Biochemistry 2021Quote: Stem sections were cut by hand from the basal third of stems of 6-week-old plants and were stained in 0.01% toluidine blue O (Sigma-Aldrich; T3260) for 2 min ...
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bioRxiv - Cancer Biology 2021Quote: ... each well of 6 well-plate were transferred to a 10cm plate with previously determined concentrations of puromycin (Sigma-Aldrich, USA), for that cell line for 3 days ...
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bioRxiv - Neuroscience 2021Quote: ... on 6 male Wistar (250–400 g; Charles Rivers) by a single intraperitoneal (IP) injection of pilocarpine (320 mg/kg; Sigma-Aldrich), one week after receiving the animals from the vendor ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was suspended in 10 ml of Histopaque 1100 (5 ml histopaque 1077; Sigma 10771 and 6 ml histopaque 1119; Sigma 11191), centrifuged for 20 minutes at 1500 rpm at 4°C and the pellet washed 2 times with QB ...
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bioRxiv - Cell Biology 2020Quote: CFs subjected to transfection with NT or anti-Col 6 siRNA were incubated for 10 min on ice in RIPA Buffer (Sigma-Aldrich). Samples were then centrifuged at 13,000xg for 15 minutes and supernatants were collected ...
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bioRxiv - Microbiology 2020Quote: ... which was added to the growth media from an Fe(III)-citrate solution prepared by mixing Fe(III)Cl3 (6 mM) and citric acid (12 mM) powders (Sigma-Aldrich) in Milli-Q water ...