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Citations for Millipore Sigma :
9851 - 9900 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... a single organoid per well was transferred on 6 well plates on day 77 and 120 in Diff2 medium with 20 ng/ml LPS serotype O111:B4 (Sigma # L2630) and incubated for 24 h ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... cells were cultured for 6 hrs either in medium alone or in the presence of retinoic acid (100 nM, Fluka, Sigma-Aldrich, Zwijndrecht ...
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bioRxiv - Cell Biology 2021Quote: ... each piece of frozen tissue was placed into one well of a 6-well plate with salt-Tris buffer containing 146 mM NaCl (Cat#S6546-1L, Sigma-Aldrich), 1 mM CaCl2 (Cat#97062-820 ...
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bioRxiv - Biochemistry 2022Quote: ... Sample was then concentrated 6 times by ultrafiltration using a 3 kDa molecular weight cut off Centricon centrifugal filter units (EMD Millipore) and applied onto a Superdex 200 10/300 increase (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: ... as previously described.23 The isolated tissues were incubated in cell dissociation solution comprising of 6 mg papain (P4762, Sigma, USA) in 3 ml of Hibernate E-Ca (HE-Ca ...
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bioRxiv - Cancer Biology 2022Quote: ... TILs and CAR-TILs were co-cultured with cancer cells for 4-6 hours in RPMI supplemented with human AB serum (Sigma-Aldrich) and CD107a antibody (clone H4A3 ...
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bioRxiv - Biochemistry 2022Quote: ... and a final SEC polishing step was performed on Superose® 6 10/300 Increase (Millipore Sigma, catalog # GE17-5172-01) pre-equilibrated in 10 mM HEPES pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was immobilized on a nitrocellulose membrane and then probed with antibodies against (6-4)PPs (Cosmo Bio NM-DND-002) or ssDNA (Millipore MAB3034). Chemiluminescence was detected as for protein immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... Anti alpha tubulin-FITC mouse mAb (#F2168) and 4’,6-diamidine-20-phenylindole dihydrochloride (DAPI, #10236276001) were purchased from Sigma Aldrich, Germany ...
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bioRxiv - Cell Biology 2022Quote: With single hand restrain mice starved for 6 hrs were administered intra-peritoneally with 50mg/kg body weight of streptozotocin (Sigma Aldrich) for 6 consecutive days ...
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bioRxiv - Genomics 2022Quote: ... a single thymidine block was used to first arrest cells in S phase: cells were plated at 4×10^6 cells per 15 cm plate in medium containing 2mM Thymidine (Sigma, T1895), and were grown for 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... cells seeded in 12-well plates were pre-treated with 500 mU/mL α2-3/6/8 sialidase from Clostridium perfringens (Sigma-Aldrich) for 3 hours at 37°C before infection ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated in 24-,12- or 6-well plates pre-coated with 10 μg/ml of poly-L-lysine (Sigma-Aldrich) and maintained at 37°C in a humidified atmosphere containing 5% CO2.
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bioRxiv - Neuroscience 2022Quote: HEK293 cells stably expressing FLAG-tagged D3R were grown in DMEM with 10% FBS in 6-well plates coated with 400 µl ECM (Sigma E1270) to 80-90% confluence ...
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bioRxiv - Neuroscience 2022Quote: Embryos and larvae obtained from TL crosses were raised to the desired ages (30 hpf, 48 hpf, 6 dpf) and stored in RNAlater (Sigma, #MKCL5657). Total RNA was extracted from 10 dissected eyes and 10 dissected heads without eyes at each age using a phenol chloroform extraction method with TRI-Reagent (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... 6; MgCl2, 4; CaCl2, 5; sucrose, 160; glucose, 25; Hepes, 10; pH 6.7, 500 mOsmol; all chemicals from Sigma-Aldrich, France), to avoid desiccation of the brain surface ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plates was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plate was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Breeding pairs of all mice strains (6 weeks old) were fed with a chow diet containing 0.012% TCS (Sigma-Aldrich, 72779) dissolved in vehicle (2ml DMSO + 10ml water ...
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bioRxiv - Molecular Biology 2022Quote: ... dechorionated embryos were crosslinked in a mixture of 2 ml fixation buffer (PBS, 0.5% Triton X-100) and 6 ml heptane supplemented with 100 μl of 37% formaldehyde (Sigma-Aldrich, F8775) for 15 min at room temperature with rotation ...
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bioRxiv - Neuroscience 2022Quote: ... or the same volume of vehicle containing ghrelin receptor antagonist (GRA; [D-Lys3]-GHRP-6; 3.3mg/kg BW; Sigma-Aldrich G4535). This GRA dose was based on our previous report demonstrating its ability to restore cholecystokinin-induced NTS neuronal activation in food deprived rats [20] ...
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bioRxiv - Bioengineering 2022Quote: ... cell-laden microgels were demulsified and resuspended by an excessive amount of LB media spiked with 10−6 mol/L 3-Oxo-C12-HSL (Sigma-Aldrich) before incubation at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... After the addition of the appropriate amount of the 6× Laemmli Sample Buffer (Bioland scientific LLC, sab03-02) with 5% ß-mercaptoethanol (Sigma) protein samples (10-30 μg ...
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bioRxiv - Cell Biology 2022Quote: ... human very-low-density and low-density lipoproteins (catalog no. 437647-5MG and 6 mg/ml, LP2-2MG, Merck Millipore, respectively) were purchased and stored according to the manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Cochlear explants were cultured for 6 days in DMEM with 10% fetal bovine serum and 10 µg/ml ciprofloxacin (Sigma, 17850). Explants were then fixed in 4% PFA for 15 min at room temperature before being processed for immunolabeling.
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bioRxiv - Cell Biology 2024Quote: ... virus was collected and used to infect NIH3T3 cells with 6 µg/ml polybrene (Sigma, St Louis, MO, USA; #TR-1003) for another 12 h ...
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bioRxiv - Biochemistry 2024Quote: ... mice were fasted for 6 hours (for GTT) or 5 hours (for ITT) and received an intraperitoneal injection of glucose (1g/kg, Sigma Aldrich) or insulin (0.75 units/kg ...
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bioRxiv - Molecular Biology 2024Quote: ... for differentiation induction 100.000 cells were plated in a 6-well plate and maintained in complete media supplemented with 20μM retinoic acid (Sigma-Aldrich, R2625) in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... GV oocytes were collected from the ovary of 3-4 weeks old C57BL/6 females 48 h after PMSG injection and then were incubated in M2 medium (Sigma, #M7167) supplemented with 10 μM Cilostamide (TargetMol ...
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bioRxiv - Immunology 2024Quote: Bone marrow-derived macrophages were prepared from C57BL/6 and Rel-/-mice as described.68 Macrophages were activated on day 6 with lipid A for the time indicated in each experiment (Sigma-Aldrich). Nascent transcript RNA-seq was performed and the data were analyzed as described.68,69
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bioRxiv - Neuroscience 2024Quote: ... Slides containing the sections were immersed for 40 min in 10 mM citrate buffer pH 6 (sodium citrate, Sigma Aldrich, Germany) at 96°C in a water bath ...
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bioRxiv - Microbiology 2024Quote: ... approximately 7×105 cells growing on 6-well plates were washed with PBS and harvested with Tri Reagent (Sigma Cat#T9424). Total RNA was extracted ...
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bioRxiv - Neuroscience 2024Quote: Mice 4-6 weeks old were given three doses 150 mg/kg or 200 mg/kg (i.p) tamoxifen (TAM) (Sigma-Aldrich T5648) dissolved in peanut oil (20 mg/ml ...
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bioRxiv - Cell Biology 2024Quote: MSCs were seeded overnight on 24 or 6 well plates in normal α-MEM supplemented with 10% fetal bovine serum (FBS, Sigma), 2 mM glutamine ...
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bioRxiv - Microbiology 2024Quote: ... with the same combination of primary and secondary antibodies: mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and rabbit anti-HA tag antibody (Cell Signalling Technology ...
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bioRxiv - Microbiology 2024Quote: ... The following mixture of primary antibodies was used: a mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and a rabbit anti-HA tag antibody at a 1:50 dilution (Cell Signalling Technology) ...
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bioRxiv - Immunology 2022Quote: 5–7-weeks old C57BL/6 mice bearing GL261 or CT-2A tumors were treated with KDM6B inhibitor (GSK-J4; Sigma-Aldrich); 1mg per mouse ...
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bioRxiv - Neuroscience 2023Quote: ... The spinal column was removed and 4-6 lumbar level DRG were dissected and placed onto coverslips coated with Poly-L-Lysine (Sigma, P8920). DRG were allowed to recover for 8 minutes (P4 ...
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bioRxiv - Neuroscience 2023Quote: ... Western Blotting experiments on viral particles were carried out on the collected culture media concentrated with Vivaspin 6 MWCO 30 kDa membranes (Sigma Aldrich). Samples were then reduced and denatured by boiling in 4X Laemmli sample buffer at 95°C for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... cell colonies were grown on Matrigel-coated 6-well plates (Matrigel: Cat No. 354277, Corning; Plates: Cat No. CLS3516-50EA, Sigma Aldrich) in mTeSR Plus for at least three passages before being dissociated for downstream differentiation protocols ...
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bioRxiv - Neuroscience 2023Quote: ... the SCG neurons of postnatal day 1–2 NMRI strain mice or DRG neurons from E16 embryos were grown 6 DIV on poly-L-ornithine-laminin (P3655 and CC095, Sigma-Aldrich) coated dishes with 30 ng/ml of mouse NGF (G5141 ...
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bioRxiv - Plant Biology 2023Quote: ... followed by wash with 100% ethanol twice and sown on 1X MS medium (PT011, HiMedia Laboratories, India) containing 0.025% ethanol (Mock) or 6 μM dexamethasone (DEX) (D4902, Sigma-Aldrich, USA). After sowing ...
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bioRxiv - Neuroscience 2023Quote: Induced microglia-like cells were treated for 6 and 24h with 100ng/ml of LPS from Escherichia coli (E. coli) O55:B5 (Sigma L2880) that was diluted first in DPBS and then added to the iMG media ...
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bioRxiv - Immunology 2023Quote: ... Each group (n = 6) was immunized intraperitoneally with peptides-KLH (10 μg/mouse) emulsified in Incomplete Freund’s Adjuvant (Sigma, Cat.#F5506), and a control group (n = 6 ...
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bioRxiv - Immunology 2023Quote: ... IDE12 cells were also grown to confluency in T25 flasks and either seeded at 1x106 cells/well in 6-well plates (Millipore Sigma) or 3x105 cells/well in 24-well plates (Corning) ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... motoneurons grown for 6 DIV on laminin-111-coated glass coverslips were treated with 10 µg/mL puromycin (P8833, Sigma-Aldrich) for 10 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Protein was further purified by size exclusion chromatography using gel-filtration column (Superose 6 increase 10/300 GL, Cytiva, Sigma-Aldrich) in a buffer containing 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... The remaining exposed surface was blocked for 5 minutes with 6% Pluronic F-127 (w/v) (Sigma P2443. MW: 12.5 kDa) and 15mg/ml of Bovine Serum Albumin (BSA ...
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bioRxiv - Biochemistry 2022Quote: ... Pellets were resuspended in lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM MgSO4, 5 mM 6-aminocaproic acid (Sigma, cat. #A2504), 5 mM benzamidine (Sigma ...
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bioRxiv - Biochemistry 2022Quote: EMSAs for the SAP-domain were performed with DNA such that the single strand or one of the two strands in dsDNA was 5’-labeled with fluorescein (6-FAM, Sigma-Aldrich) (Table 1) ...