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Citations for Millipore Sigma :
951 - 1000 of 2392 citations for bta mir 486 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Blots were stripped for 15 min at RT in ReBlot Plus Strong solution (Millipore #2504), blocked for 30 min in 5% blotto ...
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Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibersbioRxiv - Cell Biology 2020Quote: ... followed by incubation for 30 min at RT with anti-laminin (1:100; Sigma L9393). Secondary AlexaFluor antibodies (1:200 ...
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bioRxiv - Biophysics 2019Quote: ... in the medium for 10 min at RT and quenched with 2.5 M Glycine (Sigma) for 2 min at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... and cross-linked for 10 min at RT by the addition of formaldehyde (F1635, SIGMA) to a final concentration of 1.2% ...
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bioRxiv - Neuroscience 2021Quote: ... Blocking was performed at RT with 5% bovine serum albumin (BSA) (Sigma-Aldrich, MO, USA) in PBS ...
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bioRxiv - Immunology 2021Quote: ... blocked for 1 hr at room temperature (RT) in 5% Skim Milk Powder (Sigma-Aldrich) in TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... sections were subjected to 0.5 % hydrogen peroxide solution at RT for 20 mins (H1009, Sigma) in methanol to quench endogenous peroxidases ...
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bioRxiv - Developmental Biology 2022Quote: ... were fixed for 15 min at RT in 4% (w/v) paraformaldehyde (Sigma, Cat. #158127) and permeabilized with 0.1% Triton-X-100 (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Splenic B cells and bone marrow tissue cells were treated with RNAzol RT (Sigma-Aldrich), and total RNA was isolated according to the manufacturer’s instruction and saved at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... Sections were incubated in primary antibody for 1 hour at RT: rabbit Anti-GLUT1 (Millipore), chicken Anti-GFP (Aves) ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were further washed at RT for 5min with 1mL 100µM Ammonium bicarbonate (Sigma-Aldrich), 10mM DTT and then with 1mL 100µM Ammonium bicarbonate ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR was performed with SYBR Green Quantitative RT-qPCR Kit (Sigma-Aldrich; Cat. No. QR0100) using GAPDH as a housekeeping gene ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated overnight at room temperature (RT) with anti-VRK3 (1/1000 #HPA056489, Sigma) and anti-Phalloidine (1/200 #8940S ...
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bioRxiv - Neuroscience 2023Quote: ... Cryosections were stabilized at RT for 5 min and blocked with 10% donkey serum (Sigma) + 0.3% Triton X-100 (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by 10 min fixation at RT by adding 20 μL of 4% paraformaldehyde (Sigma) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated for 2 h at RT in casein blocking buffer (Sigma Aldrich), then overnight at +4°C with rabbit anti-CtBP2 antibody (Abcam #ab128871 ...
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bioRxiv - Cell Biology 2023Quote: Macrophages were fixed with 4% formaldehyde for 20 min at RT in the dark (Sigma). After fixation ...
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bioRxiv - Immunology 2024Quote: ... and incubated for 1h at RT with 1% BSA + 0.3% TritonX-100 (Sigma Aldrich T9284) in DPBS ...
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bioRxiv - Plant Biology 2020Quote: RNA extraction was performed using 100 mg of frozen ground tissue (leaves) and treated with RNase-Free DNase by the On-Column DNase I Digestion Set (Sigma-Aldrich). For the subsequent analysis only RNA samples with integrity greater than 8.0 were used ...
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bioRxiv - Biophysics 2022Quote: ... A bulk calibration data was initially set up by measuring the fluorescence intensity of 41-μm-thick channels passivated with 1% Pluronic F127 (Sigma-Aldrich) and filled with antibody solutions at concentrations of 3.75 ...
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bioRxiv - Neuroscience 2021Quote: ... parental crosses to obtain experimental animals were set up on standard medium supplemented with 400 μM all-trans-retinal (ATR, Sigma #R2500) food or on a standard medium mixed with 100% ethanol for no ATR controls ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% of pre-cleared chromatin was set aside for input and 5 µg Monoclonal ANTI-FLAG® M2 antibody (to capture FLAG-tagged dCas9) or IgG (Sigma) was added to the remaining volume and then incubated at 4°C on a nutator overnight ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted using the RNeasy Mini Kit and the RNase-Free DNase Set according to the manufacturer’s protocol (Sigma-Aldrich, Switzerland). Total RNA was reverse transcribed using all-in-one RT-PCR (BioTool ...
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... The flow rate was set to 400 μL/min using a buffer system consisting of buffer A (10 mM ammonium formate (Sigma-Aldrich), 0.15% formic acid (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: Mature oocytes mounted in oil on a 22 by 40mm coverslip and set up under the microscope were treated with one or two drops of 10 µg/ml cytochalasin-D (Sigma-Aldrich) or 5% 1,6 hexanediol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... was placed above the platform to support 2 to 3 drops of high concentrated glucose on the CS+ numerical set stimuli (1.5M, Sigma Aldrich G8270) and 2 to 3 drops of EVIAN water on the CS-numerical set condition ...
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bioRxiv - Biophysics 2020Quote: ... The lipid mixture was extruded through a polycarbonate membrane with 100 nm pore size using an extruder set with holder/heating block (610000-1EA, Sigma-Aldrich). The lipid vesicle solution was stored at 4 °C and used within 2 weeks.
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bioRxiv - Bioengineering 2019Quote: ... The molecular mass of detected peaks was estimated by comparison to the elution times of PEG analytical standard (ReadyCal Set, #02393, Sigma-Aldrich) and of commercial HA with known molecular mass (2-2.2 MDa ...
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bioRxiv - Plant Biology 2020Quote: ... As negative control one set of samples was pre-incubated with 20 μM E-64 (Sigma-Aldrich, St. louis, Mississippi, USA). Subsequently ...
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bioRxiv - Synthetic Biology 2021Quote: ... all chemicals were used as by Sun et al.33 except for amino acids (L-amino acids set, Sigma #LAA21-1KT).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed and washed with FoxP3-staining-buffer-set (eBioscience) and then incubated with DNase at 37°C for 30 mins (1mg/ml in D-PBS, Sigma Aldrich), DNase-solution 30ml DNase-stock + 960ml D-PBS + 10ml MgCl2) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 520-550nm emission) and 470 nm for chlorophyll autofluorescence (filter set 50, 665-715nm emission) with cells in poly-L-lysine (Sigma Aldrich) coated 8-well μ-slides (Ibidi) ...
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bioRxiv - Microbiology 2021Quote: ... was set aside for RNA-seq analysis while 200 μL was digested with 300 U/mL micrococcal nuclease S7 (MNase; Sigma Aldrich) for 30 mins at 37°C to generate ribosome-protected fragments (RPFs) ...
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bioRxiv - Immunology 2022Quote: ... Spleens were transferred to a pre-wet cell strainer (70 μm) set in a 50-mL tube pre-coated with Sigmacote (Sigma Aldrich). Spleens were crushed using the flat end of a syringe plunger ...
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bioRxiv - Bioengineering 2022Quote: The fabrication and subsequent characterization of the dopamine sensor were based on a protocol previously published by Niagi et al.66 Carbon electrodes of a thick-film electrode set-up with an integrated silver reference electrode (S1PE, MicruX Technologies) were coated with 15 μL of a tyrosinase (Sigma Aldrich), chitosan (1% ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated for 4-16 h at 37°C with 100 μL/well of virus-specific FISH probe sets (Supplementary Table 4) and primary antibodies in hybridization buffer (10% deionized formamide, 10% dextran sulfate [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was washed and subjected to Anti-DIG-AP antibody by using DIG Wash and Block Buffer Set (Sigma, #11585762001) and immonological detection of the membrane was conducted by CDP-Star chemiluminescent substrate included in the northern blotting kit.
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bioRxiv - Cancer Biology 2022Quote: ... C2C12 cell line differentiation condition was set up by supplementing the C2C12 cells with 10% Horse Serum (Sigma Aldrich, H1138-100ML) rather than FBS for 5 days ...
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bioRxiv - Bioengineering 2022Quote: ... The mix for the tumour mass was then set into 96-well plates (Corning® Costar®, Sigma-Aldrich, Dorset, UK) and incubated in 37°C for 15 min to allow crosslinking ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted using the GenElute Mammalian Total RNA Miniprep Kit and the On-Column DNase I Digestion Set (both from Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Samples of this amniotic fluid supernatant were submitted to the ONPRC/OHSU Endocrine Technologies Core (ETC) to set up bead-based multiplex assays to analyze multiple cytokine and chemokine biomarkers using the Luminex technology (Millipore Sigma).
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bioRxiv - Genetics 2023Quote: ... each probe set was added (final concentration of 500 nM) to 20mM VRC and hybridization buffer (1% Dextran sulfate [EMD Millipore] ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then scrapped in cold PBS supplemented with protease inhibitors (Protease inhibitor cocktail set III, EDTA-free, Merck/Sigma-Aldrich) supplemented with glycine and isolated by centrifugation ...
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bioRxiv - Cancer Biology 2024Quote: ... according to manufacturer’s instructions with an additional step of DNA digestion using the On-Column DNAse I digestion set according to manufacturer’s instructions (Sigma/Merck, DNASE70). cDNA was synthesized from 500 ng of total RNA using the BIORAD iScript cDNA cDNA synthesis kit (BIORAD ...
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bioRxiv - Genomics 2023Quote: A kidney biopsy core was thawed on ice and 3-4 mm of the core were chopped with a razor blade in a petri dish on ice and homogenized using a Dounce homogenizer (D8938-1 SET, Sigma-Aldrich) in 200 µl ice-cold lysis solution and incubated on ice for 20 min with additional 3.8 ml of ice-cold lysis solution ...
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bioRxiv - Physiology 2021Quote: ... Oligo-nucleotide primers were ordered either desalted or PAGE-purified (Sigma-Aldrich, St. Louis, MO, USA) and are listed in Table S3 ...
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bioRxiv - Systems Biology 2021Quote: Single-stranded primer deoxyribonucleotides with a final concentration of 100 μM were ordered from Sigma Aldrich or Eurofins Genomics ...
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bioRxiv - Immunology 2020Quote: ... single cells were FACS sorted into 384-well plates containing 384 primers and Mineral oil (Sigma). After sorting ...
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bioRxiv - Plant Biology 2023Quote: ... At least two primer pairs were designed for each gene of interest and obtained from Sigma. Only primer pairs with amplification efficiencies over 90-100% were selected for qRT-PCR analysis (Table S1) ...
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bioRxiv - Cancer Biology 2022Quote: ... All the primers used for mRNA expression were obtained from Sigma Aldrich (St. Louis, MO, USA).Enhanced chemiluminescent (ECL ...