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Citations for Millipore Sigma :
901 - 950 of 2392 citations for bta mir 486 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... using gene-specific primers (Table S2) and FastStart Universal SYBR Green master mix (Millipore-Sigma). Reactions were run in duplicate and relative Ct values were normalized and calculated independently using the –ΔΔ Ct method for the expression of the housekeeping genes HPRT1 and RPL13A (all primers are listed in Table S1).
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bioRxiv - Cancer Biology 2022Quote: ... using gene-specific primers (Table S2) and FastStart Universal SYBR Green master mix (Millipore-Sigma). Reactions were run in duplicate and relative Ct values were normalized and calculated independently using the –ΔΔ Ct method for the expression of the housekeeping genes HPRT1 and RPL13A (all primers are listed in Table S1).
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bioRxiv - Microbiology 2023Quote: ... Primers for human H19 were used as a positive control for CTCF binding (Merck Millipore).
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bioRxiv - Immunology 2024Quote: ... The primers were used at a final concentration of 0.5 μM (Guaranteed OligosTM, Sigma-Aldrich) and the PCR program consisted of 40 cycles of denaturation at 95°C for 10 sec ...
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bioRxiv - Neuroscience 2021Quote: ... One set of slices were stained with primary antibodies for mouse monoclonal anti-GFAP (G3893; Sigma-Aldrich; 1:1,000 dilution) and rabbit anti-Iba1 (019-19741 ...
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bioRxiv - Microbiology 2019Quote: ... 50 mM Tris pH7.4 and 0.75 μL Protease Inhibitor Cocktail Set III (EMD Millipore, Pacific Center Court San Diego, USA). The samples were analyzed via western analyses ...
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bioRxiv - Biochemistry 2020Quote: ... An identical sample set was prepared at 5µM in a solution containing 35mg/mL recombinant human albumin (#A9731, Sigma-Aldrich) and incubated at 37°C for either 24hr or 48hr with gentle rotation ...
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bioRxiv - Neuroscience 2021Quote: Synaptosomes were prepared from the striatum or cortex of VGLUT1venus or DAT-Cre eGFP-expressing mice by homogenization in 1ml of ice-cold isosmolar buffer (0.32M sucrose, 4 mM HEPES pH7.4, protease inhibitor cocktail Set 3 EDTA-free (EMD Millipore Corp.)) ...
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bioRxiv - Neuroscience 2019Quote: ... The first set was stained for a marker of neuronal dendrites (MAP2 1:250, Sigma Aldrich m1408, St Louis, MO), a striatal neuron marker (DARPP-32 1:400 ...
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bioRxiv - Developmental Biology 2022Quote: ... Probe sets complementary to cycB (CG3510; 48 oligos) or egfp (32 oligos) were conjugated to Atto 647N dye (Sigma-Aldrich) or Atto 565 dye (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... Normalization of the data was performed using the set of phosphopeptide standards (added to the sample prior phosphoenrichment step; MS PhosphoMix 1, 2, 3 Light, Sigma) and by non-phosphorylated peptides identified in direct analyses.
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bioRxiv - Cell Biology 2020Quote: ... The flow rate was set to 100 µL/min using a buffer system consisted of buffer A (10 mM ammonium formate (Sigma), 0.15% formic acid (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... Normalization of the data was performed using a set of phosphopeptide standards (added to the sample prior phosphoenrichment step; MS PhosphoMix 1, 2, 3 Light, Sigma) in combination with summed peak area of non-phosphorylated peptides identified in analyses of samples without phosphoenrichment ...
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bioRxiv - Cell Biology 2020Quote: ... For ZO-1 images cultures were fixed in 4% PFA for 20 min at room temperature, followed by permeabilization (Permeabilization Buffer set, Ebioscience) and blocking with 2% BSA solution (Sigma). Cells were stained with anti-ZO-1 mouse mAb (Thermo-Fischer ...
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bioRxiv - Immunology 2021Quote: ... Samples were tested by the Cytokine Reference Laboratory (University of Minnesota) using the magnetic bead set PRCYTOMAG-40K (EMD Millipore). Samples were analyzed for Non-Human Primate (NHP)-specific TNFα ...
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bioRxiv - Cell Biology 2022Quote: ... The flow rate was set to 400 µl/min using a binary buffer system consisting of buffer A (10 mM ammonium formate (Sigma), 0.15% [v/v] formic acid (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... The flow rate was set to 500 µl/min using a quaternary buffer system consisting of buffer A (5 mM ammonium acetate, Sigma) in LC-MS-grade water (Optima-Grade ...
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bioRxiv - Neuroscience 2022Quote: ... The flow rate was set to 400 μl/min using a binary buffer system consisting of buffer A (10 mM ammonium formate (Sigma), 0.15% [v/v] formic acid (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... in 100µl of binding/wash buffer supplemented with protease inhibitor with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...
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bioRxiv - Biochemistry 2022Quote: ... cells were harvested by centrifugation and were resuspended in SET Buffer comprising 200 mM tris(hydroxymethyl)aminomethane (Tris, Sigma Aldrich), pH 8.00 ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using GenEluteTM Mammalian Total RNA Miniprep Kit and On-Column DNase I Digestion Set (both Sigma-Aldrich) and a total of 9 samples fulfilling the submission requirements (0.5-1.0μg total RNA in nuclease free dH2O with A260/A280 and A60/A230 ratios above 1.8 ...
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bioRxiv - Physiology 2023Quote: ... The flow rate was set to 400 µL/min using a binary buffer system consisting of buffer A (10 mM ammonium formate (Sigma), 0.15% [v/v] formic acid (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The flow rate of 400 µl/min was set using a buffer system consisted of buffer A (10 mM ammonium formate (Sigma), 0.15% formic acid (Sigma ...
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bioRxiv - Immunology 2024Quote: ... Iron free media was supplemented with set concentrations of human holotransferrin (R&D systems, 2914-HT-001G/Sigma Aldrich, T0665) ranging from 0.0002-0.625 mg/mL ...
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bioRxiv - Plant Biology 2024Quote: ... in accordance with the manufacturer’s protocol and treated with genomic DNAse using DNASE 70-On Column DNase I Digestion set (Sigma-Aldrich) to eliminate DNA contamination ...
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bioRxiv - Neuroscience 2024Quote: ... The flow rate was set to 400 µl/min using a binary buffer system consisting of buffer A (10 mM ammonium formate (Sigma), 0.15% [v/v] formic acid (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... whole cell extracts were obtained by resuspending cell pellets in cell extract buffer (20 mM Tris [pH 7.0], 1× Protease Inhibitor Cocktail Set I (Sigma-Aldrich)) and transferred to sterile screw cap tubes containing silica beads ...
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bioRxiv - Cancer Biology 2021Quote: ... FastStart PCR Kit (Sigma-Aldrich) was used for PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... KAPA SYBR PCR kit (Sigma) was used for QPCR (Light Cycler 480 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1×PCR buffer (Sigma P2192), 400 nM of primers (Table S.6 ...
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bioRxiv - Microbiology 2023Quote: ... 1× PCR Buffer (Sigma-Aldrich), 3 mM MgCl2 ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were purified using GenElute™ PCR Clean-Up Kit (Sigma-Aldrich, USA) followed by cloning of the same using pGEM-T Easy Vector System I ...
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bioRxiv - Developmental Biology 2022Quote: ... Several probes were produced by PCR amplification (KAPA2G Fast ReadyMix PCR kit, Sigma Israel, KK5101). cDNA templates were synthesized by RNA precipitation followed by reverse transcription PCR ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... PCR products were purified with the High Pure PCR Product Purification Kit (Sigma Aldrich, 11732676001) and cloned into the pcDNA3.1 Hygro+ mammalian expression vector (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5% w/v Alizarin Red S (Sigma-Aldrich, St. Louis, USA, 10 min incubation, RT) in distilled water was used to stain the mineralized extracellular matrix (ECM) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were blocked for 30 min at RT in 1% BSA (Millipore Sigma, #A-4503) in PBS ...
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bioRxiv - Immunology 2019Quote: ... Eluted A20 was stabilized by incubation with iodoacetamide (Sigma; 30 mM, 30 min at RT). The reaction was terminated by addition of an equivalent amount of β-mercaptoethanol ...
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bioRxiv - Pathology 2019Quote: ... Cells were washed 3x 5min each at RT in PBS-T (0.1% Tween20 (Sigma P9416). Secondary was performed at RT in the dark for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... at RT under agitation followed by quenching by addition of 0.125 M Glycine (Sigma G8898) for 5 min under agitation ...
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bioRxiv - Neuroscience 2021Quote: ... Blocking was performed for 1h at RT using 20% normal goat serum (S26, Sigma-Aldrich) in Tris-NaCl blocking buffer (TNB) ...
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bioRxiv - Neuroscience 2020Quote: ... then washed overnight at RT with PBS containing 1% (v/v) Triton-X 100 (Sigma) and then with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 h each) at RT and then incubated in 66% dichloromethane (DCM, Sigma Aldrich)/33% MetOH overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were stripped for 15 min at RT in ReBlot Plus Strong Solution (Millipore #2504), blocked for 30 min in 5% BLOTTO ...
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bioRxiv - Cell Biology 2021Quote: Coverslips were coated at RT with 5ug/mL Poly-L-lysine solution (Sigma-Aldrich, P4707) for 30 minutes ...
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bioRxiv - Systems Biology 2020Quote: RT-qPCR was carried out using FastStart Universal SYBR Green Master Mix (Rox) (Sigma Aldrich) in an Applied Biosystems QuantStudio 6 ...
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bioRxiv - Microbiology 2020Quote: ... Mannose inhibition: biotinylated HHL was incubated with methyl mannose (200 mM; 30 min, RT; Sigma) prior to HHL staining.
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bioRxiv - Immunology 2021Quote: ... RT overnight) and trimethylsilylation (20μl of N,O-bis(trimetylsilyl)trifluoroacetamide + 1% trimethylchlorosilane) (Sigma, 33148) for ≥ 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... washed with 3x PBS at RT and stained with alizarin red S (Sigma Aldrich, A5533) 1% in ddH2O ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 h each) at RT and then incubated in 66% dichloromethane (DCM, Sigma Aldrich)/33% MetOH overnight ...
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bioRxiv - Immunology 2019Quote: ... RT overnight) and trimethylsilylation (20μl of N,O-bis(trimetylsilyl)trifluoroacetamide + 1% trimethylchlorosilane) (Sigma, 33148) for ≥ 1 h ...