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Citations for Millipore Sigma :
951 - 1000 of 7677 citations for CEA Calibrator Grade Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... the protein extract was incubated with 30 μL Protein A Agarose beads (Millipore), 10 μL anti-SIZ1 antibody with rotation at 4°C for 4 h.
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bioRxiv - Microbiology 2023Quote: ... The protein was purified using a Protein A agarose gravity column (Millipore Sigma) followed by SEC using a S200 column.
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bioRxiv - Cancer Biology 2023Quote: Oxidized proteins were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma, S7150) and following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were determined with the BCA protein assay kit from Sigma-Aldrich.
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified using bicinchoninic acid (BCA) Protein assay (Sigma-Aldrich). Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham ...
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bioRxiv - Genetics 2024Quote: ... protein concentration was measured by use of a BCA Protein Assay (SIGMA Aldrich) and samples stored at -80°C.
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bioRxiv - Genomics 2024Quote: ... Protein transfer and total protein content was assessed by Ponceau Staining (Sigma Aldrich). Ponceau Stain was washed out with 1x TBST ...
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bioRxiv - Molecular Biology 2024Quote: The protein mixtures were filtered using a low protein binding filter (Millipore Sigma) and purified on a SourceQ15 anion exchange column (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: Protein-G (Protein G Sepharose® 4 Fast, cat# GE17-0618-01) or Protein-A (ProteinA-Sepharose® 4, cat#P9424 Millipore) beads were washed twice with 1x PBS and twice with IP100 buffer (25 mM Tris-HCl 7.9 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Steidle and Dettner, 1993) in 70 μl hexane (company, GC/MS analytical grade) containing 150 ng/μl n-octadecane (Sigma Aldrich) as an internal standard ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were maintained and cultured using cell culture-grade plastic flasks coated for 2 hours with a mixture consisting of 40 μg/cm2 chondroitin sulfate (Sigma-Aldrich), 40 ng/ cm2 laminin (L4544 ...
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bioRxiv - Molecular Biology 2019Quote: ... The digested and purified RNA was finally dried using an Eppendorf Vacufuge and reconstituted with 70% acetonitrile (LC-MS grade; Sigma Aldrich) to a final concentration of 0.5 μg/μl ...
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bioRxiv - Genomics 2020Quote: ... overlaid them with one drop (approx. 25μl) of mineral oil (light mineral oil, BioReagent grade for molecular biology, Sigma Aldrich, USA), and stored them at −80°C until WGA ...
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bioRxiv - Bioengineering 2021Quote: ... Phosphate-buffered saline (PBS, P4417) in tablets and a Grade I Glutaraldehyde solution (G5882) specifically purified for EM were purchased from Sigma-Aldrich. If not stated otherwise ...
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bioRxiv - Bioengineering 2020Quote: ... Gels were removed from the freezer and a 1 mL solution of equal volumes HPLC-grade methanol (Fisher Chemical) and acetone (Sigma Aldrich) was added to each sample ...
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bioRxiv - Plant Biology 2020Quote: ... with complete immersion of the caryopsis in a 50% sulphuric acid solution (ACS reagent grade H2SO4, Sigma-Aldrich, St Louis, USA) for an optimal interval allowing for the weakening of floret structures without reducing germination potential ...
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bioRxiv - Microbiology 2021Quote: ... of a nucleic acid extract of each water type as well as into reagent-grade water (Milli-Q, Water Purification System, Millipore, Ontario) to serve as a positive control and subject to PCR as described above ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were obtained by centrifugation at 4000 g for 5 mins and resuspended into cold extraction buffer (acetonitrile/MeOH/H20 – 50:40:10; LC-MS grade) adding purified c-di-GMP (Sigma Aldrich) as an internal standard ...
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bioRxiv - Cancer Biology 2021Quote: ... the remainder of the solution following removal of aqueous phase was mixed with 300ul of Back extraction buffer (4M Guanidine Thiocyanate, 50mM Sodium Citrate dihydrate, 1M Tris free base, and molecular grade water. All reagents from Sigma Aldrich) per 1ml of triazol ...
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bioRxiv - Neuroscience 2022Quote: ... At the end of the incubation period (1h at RT) bound and free ligands were separated by rapid filtration over Whatman brand Grade GF/C glass filters (Sigma-Aldrich) using a sampling vacuum manifold (MilliporeSigma) ...
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bioRxiv - Plant Biology 2021Quote: The glass fibre filter papers (GFFPs) used to collect each sample were submerged in 7-mL volumes of HPLC-grade methanol (>99.9%, Millipore-Sigma Canada) in 20-mL scintillation vials (Chromatographic Specialties Inc. ...
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bioRxiv - Immunology 2020Quote: ... Protein pellets were obtained by centrifugation at 12,000 rpm for 15 minutes at 4 °C and subjected to trypsin digestion with sequencing grade trypsin (1:20) (Sigma Aldrich) overnight at 37 °C.
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bioRxiv - Immunology 2021Quote: Mice were sensitized to 20 g ovalbumin (OVA) (Grade V, 2.25 mg alum in saline, pH 7.4; Sigma, St. Louis, MO) by intraperitoneal (i.p. ...
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bioRxiv - Microbiology 2022Quote: ... and 20 uL of 120 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (Pierce; dissolved in 47% ACN, 47% water and 6% HPLC-grade pyridine (Sigma Aldrich)) were added ...
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bioRxiv - Cancer Biology 2019Quote: ... (Vienna, Austria) and 950 µl extraction solvent were added (80% MeOH, 20% H2O, both LC-MS-grade (Sigma Aldrich, Vienna, Austria)) ...
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bioRxiv - Cancer Biology 2020Quote: ... with/without anti-Flag antibody then incubated (1h at RT) with nanogold-coupled secondary antibody and finally fixed with 2.5% glutaraldehyde EM grade (Sigma Aldrich, #16210) for 1 h ...
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bioRxiv - Systems Biology 2020Quote: ... and sonic disruption were precipitated with trichloroacetic acid.69 The precipitated protein was then resolubilized in urea and treated with dithiothreitol and iodoacetamide to reduce and block disulfide bonds prior to digestion with sequencing-grade trypsin (Sigma-Aldrich). Following two-rounds of proteolysis ...
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bioRxiv - Molecular Biology 2019Quote: To the remaining 75 µL aliquot, 225 µL of ice-cold MeOH (LC-grade, Honeywell) containing the following internal standards (all from Sigma Aldrich): Heptadecanoic acid (5 ppm) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was chopped into small pieces and transferred into a 15 mL plastic tube containing 10 mL of extraction solvent consisting of isopropanol (LC/MS grade, Carlo Erba) with N-decane (purity 99%, Sigma-Aldrich) (50 μg/mL ...
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bioRxiv - Synthetic Biology 2021Quote: ... Samples were digested for 4 h on a filter with 1:50 Lys-C (Wako) and thereafter overnight with 1:50 proteomics-grade dimethylated trypsin (Sigma-Aldrich) as described for the filter-aided sample preparation protocol 42 ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... a polytetrafluoroethylene mold made by milling was used to cast 36 hemispherical wells on a thin layer of 2% agarose (Cell culture grade, Sigma-Aldrich) in ddH2O in a 35×10mm petri dish ...
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bioRxiv - Microbiology 2021Quote: ... and Acetonitrile (ACN; #34851) in High performance liquid chromatography (HPLC)/analytical grade and Phosphate-buffered saline (PBS; #P5493) were purchased from Sigma-Aldrich.
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bioRxiv - Immunology 2021Quote: ... iCD4TGFBR2 and WT C57Bl/6J mice were sensitized by i.p.-injections of 20 μg ovalbumin (OVA; grade V; Sigma-Aldrich, USA) in phosphate buffered saline (PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was pipetted to a new 1.7 mL centrifuge tube and the acetonitrile was evaporated with warm nitrogen gas in an AutoVap (Zymark) at 70° C and then reconstituted in enough HPLC grade water (Sigma-Aldrich) for a 1:5 dilution and vortexed for 20 s.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed twice with PBS and extracted with mass spectrometry grade 80% methanol (Fisher, A456-1) and 20% water (Fisher, W6500) supplemented with 1 nmol DL-Norvaline (Sigma, N7502). Flash frozen tumors/tissues were pulverized with liquid nitrogen in a mortar and pestle on dry ice ...
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bioRxiv - Cancer Biology 2022Quote: ... 400 µg protein equivalents of tissue were extracted with mass spectrometry grade 80% methanol (Fisher, A456-1) and 20% water (Fisher, W6500) supplemented with 1 nmol DL-Norvaline (Sigma, N7502). Tissue or Insoluble material from cell extracts were used to calculate protein equivalents by resuspension in 0.2 M NaOH ...
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bioRxiv - Bioengineering 2022Quote: ... 0.2 μL of reverse primer (both at 10 μmol L- 1) and 7.6 μL of molecular grade water (Sigma Aldrich, USA). Three qPCR technical replicates were prepared for each sample ...
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bioRxiv - Plant Biology 2022Quote: Chitin powder from shrimp shells (practical grade, product code: C7170) and chitosan (medium molecular weight, product code: 448877) were purchased from Sigma Aldrich, US ...
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bioRxiv - Microbiology 2022Quote: ... Samples were first incubated for 15 min at room temperature with Glutaraldehyde 50% (electron microscopy grade, Sigma-Aldrich, cat No. 340855) at 0.1% (final concentration) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CFP was cut into small pieces and transferred into a 15 mL plastic tube containing 10 mL of extraction solvent consisting of isopropanol (LC/MS grade, Carlo Erba) with N-decane (purity 99%, Sigma-Aldrich) (50 μg/mL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Beetles were then freeze-killed at −20 °C and shaken in 65 μL ice-cold acetone (LC-MS grade, Sigma-Aldrich) containing 0.05 mg mL-1 octadecane (98.5% ...
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bioRxiv - Zoology 2022Quote: ... Cuticular compounds were therefore extracted by immersing 10 living flat female ticks in 200 μl of pentane (HPLC grade, Sigma-Aldrich) in glass vials ...
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bioRxiv - Cell Biology 2023Quote: ... The fixed samples were washed on a 100 µl drop of PBS and fixed again on a 50 µl drop of 1% glutaraldehyde in PBS (electron microscopy grade, Sigma‒Aldrich) for 5 min ...
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bioRxiv - Genomics 2023Quote: ... On-bead digestion was performed by resuspending beads in 100 μL of ABC containing 1 µg of sequencing grade trypsin (T6567, Sigma-Aldrich). Samples were gently mixed at 37 °C overnight ...
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bioRxiv - Physiology 2023Quote: ... butyric acid (≥99.5% purity) and propionic acid (≥99.5% purity) were of analytical grade purchased from Sigma-Aldrich (St. Louis, MO, USA). Ammonium formate (≥99.995% purity) ...
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bioRxiv - Neuroscience 2023Quote: ... were blocked with 5% blotting grade nonfat milk (Lab Scientific, #M0841) in tris buffered saline buffer with 0.1% tween 20 (Sigma-Aldrich, #P1379). The following primary antibodies (company ...
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bioRxiv - Cell Biology 2023Quote: ... on ice in LCMS-grade acetonitrile/methanol/water (2:2:1 v/v) with 1nmol scyllo-inositol internal standard per sample (Sigma Aldrich). Sample debris was then removed via centrifugation and polar metabolite extracts were dried using a SpeedVac Vacuum Concentrator ...
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bioRxiv - Genetics 2023Quote: ... 10-200 mg tissue were digested in 1000 µl 70% trace-metal-grade nitric acid (Fisher) at 65°C for two hours then diluted 25-fold in MilliQ water (Millipore Sigma) and analyzed by ICPES (ThermoScientific iCAP 7400 DUO ...
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bioRxiv - Biophysics 2023Quote: ... The glass nanopore was then immersed in a similar buffer with the addition of 50% (w/v) Polyethylene Glycol (PEG) 35 kDa (ultrapure grade, Sigma Aldrich). The notation of the 50% PEG in the text refers to the utilization of 50% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... molecular grade), and anti-solvent, chloroform (anhydrous, ≥99%, containing 0.5-1.0% ethanol as stabilizer) were all purchased from Sigma-Aldrich (Burlington, MA).