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Citations for Millipore Sigma :
801 - 850 of 7677 citations for CEA Calibrator Grade Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 1uM each of gRNA (IDT) were mixed with spCas9 at 3:2 ratio in embryo grade water (W1503, Sigma) and injected into wild type C57BL6 zygotes at one-cell stage ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was discarded and proteins were digested by resuspension in 100 μL 100 mM ammonium acetate with 10 mM dithiothreitol and 0.5 μg trypsin (Proteomics grade, Sigma), and incubation at 37 °C with shaking for 16 h.
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bioRxiv - Neuroscience 2021Quote: ... The sample was then diluted 1:1 with HPLC-grade water and filtered using a Millex LG 0.2 μm syringe filter (Millipore) into an amber HPLC vial ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We added 1 ml methanol (HPLC grade) containing 0.01 mg of the internal standard digitoxin (Sigma-Aldrich, Taufkirchen, Germany) to freeze-dried specimens of L ...
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bioRxiv - Plant Biology 2021Quote: ... were homogenized in liquid nitrogen using mortar and pestle and 1,400 µl of 100% ice-cold methanol (HPLC grade, Sigma) was added ...
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bioRxiv - Biochemistry 2020Quote: ... A buffer of 50 mM NH4HCO3 containing 0.02 µg/µL of sequencing grade porcine trypsin (Sigma-Aldrich, MO, USA) was prepared and within 3 min of preparation 50 µL was added to the resuspended pellets ...
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bioRxiv - Plant Biology 2021Quote: ... acetonitrile and 0.1% (v/v) formic acid in HPLC grade water followed by a filtering step using 0.22 μm centrifugal filters (Millipore). Purified peptide suspensions (2 μL each ...
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bioRxiv - Microbiology 2020Quote: ... while for the plaque assay the infected cell monolayer was overlaid with Leibovitz-15 (L15) media supplemented with 0.9% agarose (DNA grade, Sigma), antibiotics and 2 μg mL−1 TPCK Trypsin ...
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bioRxiv - Biophysics 2020Quote: ... Hearts were subsequently digested for 11 minutes with the same solution supplemented with 0.08 mg/mL Liberase TM Research Grade (Sigma). Following digestion ...
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bioRxiv - Neuroscience 2022Quote: ... bound and free ligands were separated by rapid filtration over Whatman brand Grade GF/B glass filters (Sigma-Aldrich) using a sampling vacuum manifold (MilliporeSigma) ...
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bioRxiv - Microbiology 2020Quote: ... Incubations were stopped by fixing 2 ml of the culture with 2X EM grade glutaraldehyde (2.5% final concentration, Sigma) and subsequently storing at 4 °C until the sorting analysis ...
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bioRxiv - Biophysics 2021Quote: ... Cis-9-tricosene as well as chloroform and methanol (pro analysis grade) were obtained from Sigma-Aldrich (Vienna, Austria). Stock solutions of lipid (10 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... The digest was diluted 5-fold with 25 mM TEAB and digested with 0.7 ug of sequencing-grade trypsin (Sigma) overnight at 30℃ ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were resuspended in 100 μL 100 mM ammonium acetate and 10 mM dithiothreitol with 0.5 μg trypsin (Proteomics grade, Sigma) and digested at 37 °C with shaking for 16 h ...
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bioRxiv - Cell Biology 2019Quote: ... all female animals were selected and sensitized by subsequent i.p injections of 0.1 ml of OVA grade VI (1000 μg, Sigma-Aldrich) absorbed to 1 mg of imject Alum (Pierce Rockford ...
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bioRxiv - Molecular Biology 2019Quote: ... exendin-4 (E7144) and RIA-grade bovine serum albumin (A7888) were purchased from Sigma Aldrich (St. Louis, MO, USA). Anti-insulin antibodies (Insulin + Proinsulin Antibody ...
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bioRxiv - Synthetic Biology 2019Quote: ... PDYA was prepared using various laboratory grade reagents (per 0.5 L; 20 g glucose, Sigma; 10 g starch from potato, Sigma; 7.5 g Yeast Extract ...
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bioRxiv - Microbiology 2019Quote: ... each RNA sample was supplemented with 1 unit of DNase I (from the DNA amplification grade Kit of Sigma) per µg of RNA and incubated at room temperature for 10 min ...
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bioRxiv - Biophysics 2020Quote: All reagents were at least of analytical purity grade and were purchased from Sigma-Aldrich (St. Louis, MO, USA), Thermo Fisher Scientific (Waltham ...
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bioRxiv - Immunology 2020Quote: Cells were suspended in cell staining buffer (CSB - CyTOF-grade PBS, 0.625% BSA, and 25mg sodium azide; all from Sigma). 100μM cisplatin was used for viability staining (Fluidigm ...
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bioRxiv - Biochemistry 2021Quote: ... The MIDAS screening pools were prepared in LC-MS grade 150 mM ammonium acetate pH 7.4 (Sigma-Aldrich 73594) and pH-adjusted with ammonium hydroxide (Sigma-Aldrich 338818) ...
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bioRxiv - Biochemistry 2021Quote: ... Solvents and reagents used in peptide synthesis were sourced as follows: DMF and DCM (ACS grade) from Sigma-Aldrich; piperidine (99% ...
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bioRxiv - Biochemistry 2021Quote: ... 3-trimethylsilyl propionic acid (TSP) and 2,2,2-trifluoroethanol (TFE-d3) of analytical grade were obtained from Sigma-Aldrich (Ireland).
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bioRxiv - Bioengineering 2020Quote: ... according to the manufacturer’s protocol and the cDNA was diluted 1:2 in molecular biology grade water (Sigma,UK). Gene expression analysis was performed by quantitative real time PCR on a RotorGene 6000 cycler (Corbett Research ...
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bioRxiv - Cancer Biology 2020Quote: ... metabolites were resuspended in 100 μL LC-MS grade water and run on a SeQuant ZIC-pHILIC column (2.1×150 mm, 5 μm, Millipore-Sigma) on a Dionex UltiMate 3000 UPLC using a 0 to 80% gradient of 20 mM ammonium carbonate and 0.1% ammonium hydroxide ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Surface hydrocarbons were extracted from individual eggs and larvae using 10µl of n-pentane (≥99%, HPLC grade, Sigma-Aldrich) for 2 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Precipitated proteins were resuspended in 100 μL of 100 mM ammonium acetate and 10 mM DTT with 0.5 μg trypsin (Proteomics grade, Sigma) and digested at 37 °C for 16 h with shaking.
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bioRxiv - Biochemistry 2022Quote: HPLC grade methanol (34860-4X4L-R) and methoxyamine hydrochloride (226904-25G) were purchased from Sigma Aldrich (Burlington, MA, USA). Silylation Reagents ...
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bioRxiv - Physiology 2022Quote: WT mice on vivarium chow were gavaged with either pharma grade linoleic acid (catalog no. 39269-10G, Millipore Sigma) or water for 3 days using disposable animal feeding needles (catalog no ...
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bioRxiv - Cell Biology 2022Quote: ... that were spiked with 0.1% (w/v) 2,6-Di-tert-butyl-4-methylphenol (GC grade, Sigma-Aldrich, B1378-100G).
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bioRxiv - Molecular Biology 2023Quote: ... was diluted to 100 ng/μL in 6 mL nuclease-free molecular grade water (Sigma-Aldrich, St. Louis, MO) in a 15 mL tube with 300 g (50 mg/mL ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were plated on gelatin-coated (0.1% v/v) Nunc 10-cm dishes in serum-free ESGRO Complete Clonal Grade Medium (Millipore) to which was added 1,000 U/ml LIF ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were plated with high density (1.5 × 105 cells/cm2) in serum-free ES- GRO Complete Clonal Grade Medium (Millipore) to which 1,000 U/ml LIF was added ...
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bioRxiv - Cell Biology 2023Quote: ... The equivalent of 2 OD595 was quenched by direct injection into 100% -80°C LCMS-grade methanol (Sigma Aldrich). Polar metabolite extraction was adapted from a protocol described in (Doppler et al. ...
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bioRxiv - Biochemistry 2023Quote: All buffers were prepared with high purity premium grade reagents (e.g. Honeywell TraceSelect, Sigma BioXtra or EMD Millipore EmSure) to minimize introduction of extraneous metals ...
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bioRxiv - Biochemistry 2023Quote: All buffers were prepared with high purity premium grade reagents (e.g. Honeywell TraceSelect, Sigma BioXtra or EMD Millipore EmSure) to minimize introduction of extraneous metals ...
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bioRxiv - Molecular Biology 2023Quote: ZZZ3 and its interactors were released from the magnetic beads by pre-digestion in 100 µl of digestion buffer (100 mM Tris-HCl pH 8.5, 0.1% Triton X-100, 400 ng trypsin Proteomics Grade (Sigma-Aldrich) for 15 min at 37°C with shaking (500 rpm) ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracted metabolites are then resuspended in HPLC-grade water containing 5 μM 1,4-piperazinediethanesulfonic acid (PIPES; P6757, Sigma-Aldrich) as an internal standard.
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bioRxiv - Neuroscience 2023Quote: ... methyl-tert butyl ether and ammonium acetate (LC-MS grade) were purchased from Sigma Aldrich (St. Louis, MO, USA), internal standard mixture SPLASH® LIPIDOMIX® was obtained from Avanti Polar Lipids (Alabaster ...
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bioRxiv - Plant Biology 2023Quote: ... (2) addition of 15 μL of derivatization grade N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA; Sigma-Aldrich; Catalog Number 394866) and incubation at 37°C for 30 min with agitation at 750 RPM ...
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bioRxiv - Systems Biology 2023Quote: ... Mobile phase A and B were 10 mM ammonium formate (Sigma-Aldrich, >99/0%, LC-MS grade, 70221-100GF), pH 10 and 20% 10mM ammonium formate (pH 10)/80% methanol (Optima LC/MS grade ...
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bioRxiv - Physiology 2023Quote: ... 2 µL 10mM dNTPs (Thermo, R0181), 0.2 µL RevertAid reverse transcriptase (Thermo, EPO442) and 2.3 µL molecular grade water (Sigma, W4502) per well ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were flash-frozen by manipulating the cryomolds in partially frozen molecular biology grade isopentane (Sigma-Aldrich, M32631). The frozen blocks of samples may be kept at -80°C for long-term storage ...
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bioRxiv - Biophysics 2024Quote: ... Mice were decapitated and the nose was dissected en bloc and embedded in 3% low-grade agar (A7002, Sigma) prepared with Ringer’s saline solution containing (in mM) ...
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bioRxiv - Cell Biology 2024Quote: ... All solvents used were either HPLC or LC/MS grade and purchased from Sigma-Aldrich (St Louis, MO, USA). Splash Lipidomix stadndards were purchased from Avanti (Alabaster ...
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bioRxiv - Cancer Biology 2024Quote: ... 25 mM HEPES and 5 mg/ml cell culture grade BSA (Sigma-Aldrich, product number A1470; St. Louis, MO). Antibiotics used for selection included G418 ...
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bioRxiv - Cell Biology 2024Quote: The solvents used were either HPLC or LC/MS grade and purchased from Sigma-Aldrich (St Louis, MO, USA). Splash Lipidomix stadndards were purchased from Avanti (Alabaster ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected by Western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-Gsp1 from rabbit (Makhnevych et al. ...
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bioRxiv - Immunology 2020Quote: ... Protein extracts were then precleared with protein G beads (EZview™ Red Protein G Affinity Gel, Sigma-Aldrich, Israel), following incubation for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 100 mg of protein was incubated with the indicated antibodies overnight and then mixed with protein A or protein G-agarose beads (Millipore), respectively ...