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Citations for Millipore Sigma :
851 - 900 of 7677 citations for CEA Calibrator Grade Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... For pulldown of DNA-protein complex, protein-A Sepharose (G.E healthcare, 17078001) beads were precoated with anti-protein A antibodies (Sigma, P3775) and anti-HA antibodies (Roche ...
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Immunology 2022Quote: ... The concentration of protein was determined by BCA protein assay kit (Millipore). The heated protein samples were then analyzed by SDS-PAGE using 10% acrylamide/bis-acrylamide and western blot ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein concentration was determined by BCA protein assay kit (Sigma, USA). Equal amounts of the extracts were subjected to 8% SDS-PAGE ...
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bioRxiv - Cancer Biology 2019Quote: ... following by adding Protein A-Sepharose or protein G-Sepharose beads (Sigma) overnight ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein concentrations were measured using a BCA protein assay kit (Sigma) and normalized when needed by adding extra PE buffer ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA Protein Assay Kit (Sigma-Aldrich, 1001491004) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were stained using SYPRO Red Protein Gel Stain (Sigma Aldrich) according to the manufacturers’ protocols.
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested by trypsin/lysC (protein: enzyme = 25:1, Millipore) at 37°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was measured using Bradford protein assay (Sigma, Missouri, USA). The total protein extract was separated via SDS-PAGE and then transferred onto the 0.22 µm or 0.45 µm PVDF membrane (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein concentration was determined using Bradford protein assay (Sigma, Missouri, USA). The total protein was then resolved on SDS-PAGE (8-12% ...
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bioRxiv - Immunology 2023Quote: ... and immunoprecipitated with protein A and protein G sepharose beads (Millipore Sigma). After thoroughly washing beads with SLBA buffer (0.15M NaCl ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitated protein-DNA complexes were captured on protein A-Sepharose beads (Sigma) and eluted in elution buffer (1% SDS and 100 mM NaHCO3 ...
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bioRxiv - Plant Biology 2024Quote: ... After determining the protein concentration with Bradford protein assay reagents (Sigma B6916), the protein extract at 5 mg mL-1 was split into two LoBind 1.5 mL tubes and incubated with 100 µM DAPIA or equal volume of DMSO (1% v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined by BCA assay (Novagen BCA Protein Assay Kit) on a NanoDrop Microvolume Spectrophotometer (Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-HA rabbit antibody (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-FLAG mouse antibody (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... This was then followed by genomic DNA removal and cleaning using the RNase-free DNase I Amplification Grade Kit (Sigma). All RNA samples were assessed for quality using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The TAN concentrations per experimental aquaria were achieved by adding analytical grade ammonium bicarbonate solutions (NH4HCO3, Sigma-Aldrich, Barcelona, Spain). These solutions were dispensed with automatic pipettes after water changes ...
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bioRxiv - Cell Biology 2020Quote: ... Primary osteoblast precursors were isolated from the calvarias of newborn mice by sequential digestion (0.2 % dispase Grade II (Roche, Sigma-Aldrich, /collagenase Typ Ia ...
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bioRxiv - Developmental Biology 2020Quote: ... Tissue dissociation was induced through addition of an equivalent volume of Papain solution (1 ml - 700 μl reagent grade water, 100 μl fresh 50 mM L-Cysteine (Sigma), 100 μl 10 mM EDTA ...
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bioRxiv - Molecular Biology 2019Quote: Tamoxifen was administered to all experimental and control mice via intraperitoneal injections at (75 mg/kg) using pharmaceutical-grade tamoxifen (Sigma) dissolved in corn oil (Sigma) ...
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bioRxiv - Developmental Biology 2019Quote: ... Tamoxifen was administered to Rosa26-MEK1 × SKA-MCM and Rosa26-MEK1 controls via intraperitoneal injections at 2 months of age for 5 consecutive days (75 mg/kg) using pharmaceutical-grade tamoxifen (Sigma) dissolved in corn oil (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... were fixed for 15 min at 4° C in the dark with 25% electron microscopy (EM) grade glutaraldehyde (0.5% final concentration; Sigma-Aldrich), flash-frozen in liquid nitrogen and stored at −80° C until analysis.
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bioRxiv - Microbiology 2019Quote: ... The cultures were incubated for an additional 4 h at 37°C and the media was replaced with microscopy-grade media (Sigma DMEM without Phenol Red ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Frozen brain tissue was ground with a motorized pestle for 30 s in 100 µl of chilled (4:1) analytical grade methanol:ddH2O with 1 mM norluecine (Sigma Aldrich) standard ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted as per manufacturer instruction and genomic DNA was removed using the RNase-free DNase I Amplification Grade Kit (Sigma). All RNA samples were assessed for quality using an Agilent 2100 Bioanalyzer ...
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... the dried metabolite pellets were resuspended in 90 μl of the LC-MS-grade water (Milli-Q 7000 equipped with an LC-Pak and a Millipak filter, Millipore). Then ...
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bioRxiv - Neuroscience 2020Quote: ... The glass extraction chamber and gas-washing bottle were washed (Babyganics fragrance-free dish soap and DI water) and rinsed with methanol (HPLC-grade, ≥99.9%, Sigma Aldrich) and hexane (≥99.8% for GC-MS ...
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bioRxiv - Plant Biology 2021Quote: ... The compounds that were not available as analytical grade substance were purchased as the purest available substances from Sigma Aldrich: pelargonic acid (CAS 112-05-0 ...
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bioRxiv - Molecular Biology 2020Quote: ... All the chemicals and standards are analytical grade or higher and were obtained commercially from Sigma Aldrich (St. Louise, MO). Nucleotide standards were at least 98% pure.
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bioRxiv - Molecular Biology 2022Quote: ... The sample was filtered through a 0.22 μm centrifugal filter and loaded onto a HiLoad 16/60 Superdex 200 prep grade size exclusion column (Millipore Sigma) equilibrated in 25 mM Tris (pH 7.5) ...
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bioRxiv - Neuroscience 2022Quote: Headless larvae (7 dpf) were fixed for three days at 4 °C in EM fixation buffer [2% EM-grade glutaraldehyde (Sigma), 2% PFA (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: Chemicals and reagents used in this study were of cell-culture and analytical grade and were purchased from Sigma-Aldrich/Merck KGaA (Darmstadt ...
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bioRxiv - Plant Biology 2021Quote: The glass fibre filter papers (GFFPs) used to collect each sample were submerged in 7-mL volumes of HPLC-grade methanol (>99.9%, Millipore-Sigma Canada) in 20-mL scintillation vials (Chromatographic Specialties Inc. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... exendin-4 (E7144) and radioimmunoassay (RIA)-grade bovine serum albumin (A7888) were purchased from Sigma Aldrich (St. Louis, MO, USA). Anti-insulin antibodies (Insulin + Proinsulin Antibody ...
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bioRxiv - Microbiology 2021Quote: ... Frozen samples were thawed on ice for 2 min and 1 mL of HPLC grade methanol (Sigma, 34860-4L-R) added to the pellets ...
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bioRxiv - Biophysics 2021Quote: All chemicals were reagent grade and all buffers were prepared in high-quality deionized water from a Milli-Q ultrapure water purification system (Millipore) having resistivity of >18.2 MΩ.cm and were filtered again on a 0.20 μm filter before use ...
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bioRxiv - Microbiology 2022Quote: ... The slurry was centrifuged at 2,350 × g for 30 seconds at 4°C and 250 µL of the supernatant was removed to a septum-topped glass vial and acidified with 20µL HPLC grade 37% HCl (Sigma Aldrich). Diethyl ether (500 µL ...
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bioRxiv - Microbiology 2022Quote: ... 20% HPLC-grade acetonitrile (ACN; Fisher) and labelled isotopes of each SCFA measured (2.5 uM d3-acetic acid (Sigma Aldrich), 1 uM propionic-3,3,3-d3 acid (CDN Isotopes) ...
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bioRxiv - Cell Biology 2022Quote: Chemicals and solvents (analytical grade) were purchased from the following sources: α-Cyano-4-hydroxycinnamic acid 98% (CHCA) (Sigma Aldrich), 1,5-Diaminonaphthalene 97% (DAN ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated using RNAzol RT (Astral Scientific) per manufacturer’s instructions with the use of molecular grade isopropanol and ethanol (Sigma Aldrich) for RNA precipitation ...
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bioRxiv - Biophysics 2019Quote: ... The slab was then immediately immersed in a 5% (v/v) solution of APTES in HPLC-grade ethanol (Sigma, 459828) for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... One of the two dried metabolite pellets of each sample was re-suspended in 20 µl of the LC-MS-grade waters (Milli-Q 7000 equipped with an LC-Pak and a Millipak filter, Millipore). The re-suspended sample was mixed with 10 µl of 100 mM sodium carbonate (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... dichloromethane extracts were concentrated in vacuo and placed in a vial with 150 µL of dichloromethane and 2 µL of 26.7 ppb of toluene (GC grade, Sigma-Aldrich) (internal standard) ...
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bioRxiv - Microbiology 2021Quote: ... The sample was then passed through a 0.22 µm centrifugal filter and loaded onto a HiLoad 16/60 Superdex 200 prep grade size exclusion column (Millipore Sigma) equilibrated in 25 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: ... purified and its apo form prepared as described previously.41 Unless otherwise stated all chemicals were of the highest analytical grade and were purchased from Sigma, Merck or Fisher.
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bioRxiv - Cancer Biology 2021Quote: ... containing 150 μg/mL Liberase™ TL Research Grade (Cat# 5401020001, Merk) and 150 μg/mL DNase (DN25, Sigma Aldrich), for 30 min at 37°C with 400 rpm agitation ...
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bioRxiv - Microbiology 2019Quote: ... The cultures were incubated for an additional 4 h at 37°C and the media was replaced with microscopy-grade media (Sigma DMEM without Phenol Red ...