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Citations for Millipore Sigma :
51 - 100 of 731 citations for Canine Distemper Virus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Virus propagation was performed in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... for the limiting antigen condition and 2 µg/ml (4000 molecules/μm2) for the high antigen condition) and streptavidin (Sigma-Aldrich) were added to the flow chambers with or without 0.5 µg/ml unlabeled histidine-tagged ICAM-1 ...
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bioRxiv - Microbiology 2019Quote: ... with eight bi-directional PHW2000 plasmids containing the appropriate influenza A virus segments and coculture in Madin-Darby Canine Kidney (MDCK) cells (ATCC) with addition of 2μg ml−1 of TPCK treated Trypsin (Sigma-Aldrich). Rescued viruses were passaged once in embryonated hen’s eggs to generate working stocks ...
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bioRxiv - Microbiology 2021Quote: Madin-Darby canine kidney (MDCK) and human embryonic kidney 293T (293T) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich) with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: MDCK (Madin-Darby canine kidney, obtained from ATCC) were grown at 37°C in 5% CO2 in MEM medium (Sigma) containing 5% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2021Quote: Madin–Darby canine kidney (MDCK) cells were cultured in minimum essential medium (MEM; Sigma-Aldrich Co. LLC, St. Louis, MO) supplemented with 5% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... was grown in Madin-Darby canine kidney (MDCK) cells with serum-free MEM (HyClone, Cytiva) containing 1 IU/mL of trypsin (Sigma), 1 μg/mL EDTA (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen retrieval was performed in Trilogy reagent (920P, Sigma) for 10 minutes in the pressure cooker ...
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bioRxiv - Bioengineering 2022Quote: ... and Proliferating cell nuclear antigen (PCNA, Sigma Aldrich-MABE288) diluted 1 ...
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bioRxiv - Microbiology 2020Quote: ... Antigens were emulsified in complete Freund’s adjuvant (Sigma-Aldrich) in 1:1 ratio before subcutaneous injection in the animals ...
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bioRxiv - Immunology 2021Quote: ... Antigen binding was visualized with avidin-peroxidase (Sigma-Aldrich) and developing solution containing phenylenediamine (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2020Quote: ... Antigen retrieval was enzyme mediated (chondroitinase ABC, Sigma-Aldrich). Slides were blocked with Innovex Background Buster and incubated with primary antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... antigen retrieval was performed using in citrate buffer (Sigma) and sections were permeabilised and blocked 1% Triton X-100 ...
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bioRxiv - Immunology 2020Quote: Commercial antigens were double stranded DNA (cat. D3664, SIGMA), insulin (I9278 ...
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bioRxiv - Immunology 2020Quote: ... polygyrus antigen or 2 μg/ml concanavalin A (Sigma) at 37 0C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... different concentrations of antigen (Ag; DNP-HSA, Sigma, #A6661), Mastoparan (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... and heat-induced antigen retrieval in EDTA buffer (Sigma) at pH 8.5 (for Ki67 ...
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bioRxiv - Genetics 2023Quote: ... After unmasking antigens in boiling citrate buffer (Sigma, C9999) with pH 6.0 for 40 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and antigen retrieval was performed with Trilogy (Sigma-Aldrich). Slides were then stained as described above using the following antibodies ...
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bioRxiv - Molecular Biology 2021Quote: Retroviral infection was conducted in 6-well plates using either 1 mL of unconcentrated virus or 200 μL of concentrated virus in 20 mM HEPES pH 7.4 and 8 μg/mL polybrene (Sigma-Aldrich). If selection was desired ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma) for 8-12 hours ...
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bioRxiv - Neuroscience 2020Quote: Virus was produced by polyethylenimine (PEI; Sigma-Aldrich) transfection of 89-90% confluent HEK293T cells with the viral vector together with the lentiviral packaging plasmids ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... or an empty vector control virus (Sigma SHC001V) followed by puromycin (1 μg/mL ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the infected cells were fixed with cold acetone and tested using CPV-2-specific canine antibodies and rabbit anti-dog IgG conjugated with fluorescein isothiocyanate (Sigma Aldrich). Viral titre ...
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bioRxiv - Microbiology 2023Quote: Madin-Darby canine kidney (MDCK) cells were grown in Roswell Park Memorial Institute (RPMI)-1640 medium (Sigma Aldrich, Castle Hill, Australia) supplemented with 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2021Quote: ... To transduce cells with virus cells were treated with both virus (MOI 0.5-1.0) and 5 μg / mL Polybrene infection reaction (EMD Millipore, TR-1003). For pLKO.1 vectors ...
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bioRxiv - Immunology 2021Quote: ... Concentrations were determined in duplicate by fluorescent-coded magnetic beads (MagPlex-C; MILLIPLEX. MAP Kit, Canine Cytokine Magnetic Bead Panel, 96-Well Plate Assay, CCYTO-90K, Millipore, Billerica, MA), based on the Luminex xMAP technology (Luminex 200 ...
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bioRxiv - Microbiology 2022Quote: ... Madin-Darby canine kidney (MDCK) II cells (Institute of Virology, WWU Muenster, Germany) were cultured in minimum essential medium (MEM, Sigma-Aldrich, M4655) supplemented with 10 % fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2023Quote: The human megakaryoblastoid cells UT7/Epo and the canine MDCK II cells were cultured in Eagle’s minimal essential medium (MEM) (Sigma, St. Louis, MO) containing 5% fetal calf serum (FCS) ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Neural/Glial antigen 2 (NG2 - Millipore AB5320, 1:100); anti-PDGFRβ (R&D AF385 ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-flavivirus group antigen (1:800, MAB10216, Merck Millipore) and goat anti-Iba1 (1:300 ...
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bioRxiv - Microbiology 2021Quote: ... Antigen retrieval performed with sodium citrate buffer (Sigma-Aldrich C9999) and a steamer ...
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bioRxiv - Microbiology 2020Quote: ... Antigens were emulsified in Freund’s Complete Adjuvant (Sigma-Aldrich, F5881) for the first immunization or Freund’s Incomplete Adjuvant (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Antigens were retrieved using 0.1% trypsin (Sigma-Aldrich, MO, USA) and non-specific staining was blocked using 10% fetal bovine serum/neonatal goat serum for 1 hr prior 90 min incubation with either biotinylated anti-laminin antibody (Novus Biologicals ...
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bioRxiv - Immunology 2022Quote: ... Antigens were retrieved using citrate buffer (pH 6.0, Sigma-Aldrich) in a KOS MicrowaveStation (Milestone SRL) ...
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bioRxiv - Cancer Biology 2022Quote: ... The tested antigens included Cardiolipin (50 μg/mL, Sigma C0563), KLH (5 μg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... Antigen retrieval was performed using 10X Citrate Buffer (Sigma-Aldrich) in a capped coplin jar microwaved for ∼45 seconds followed by 15-minutes incubation in microwave ...
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bioRxiv - Neuroscience 2022Quote: ... neural/glial antigen 2 (NG2; OPCs; Millipore; AB5320; 1:200), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cell Biology 2024Quote: ... Antigen retrieval was performed in 1x citrate buffer (Sigma, C9999) with a pressure cooker for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... a human nuclear-specific antigen [(EMD Millipore; MAB1281 (1:1000)] ...
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bioRxiv - Molecular Biology 2022Quote: ... For each 10 cm dish, 1 ml virus inoculum was prepared in virus growth media (VGM; containing DMEM, 0.2% bovine serum albumin (Sigma; Cat no. #A8412), 25 mM N-(2-hydroxyethyl ...
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bioRxiv - Microbiology 2020Quote: ... Aggregated virus was removed by a 0.22µm filter (Millipore). Sub-confluent monolayer of MEFs cultured in DMEM ...
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bioRxiv - Physiology 2020Quote: ... Virus was filtered with a 0.45mm OVDF filter (Millipore). Supernatants were collected and purified using PEG-it (System Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... supernatant containing virus was filtered with 0.22μm filters (Millipore) and store at −80◻ in aliquots.
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bioRxiv - Cancer Biology 2023Quote: ... adherent cells were infected with COL7A1 virus (Sigma Aldrich) (1–5 MOI (multiplicity of infection) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the virus was concentrated using polyethylglycol (Sigma, P4338). Cells were infected in medium containing 5 μg ml−1 polybrene (Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... Antigen retrieval was performed boiling in pH6 Citrate buffer (Sigma-Aldrich) for 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Antigen retrieval was followed by permeabilization with 0.5% Triton-X100 (Sigma). EdU incorporation was followed by EdU Click-IT chemistry according to manufacturer’s instructions (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse-anti-flavivirus group antigen (Millipore: MAB10216; RRID: AB_827205; 1:500), pan-flavivirus hyperimmune mouse acites fluid (HMAF ...
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bioRxiv - Cell Biology 2021Quote: ... SV40 T Antigen (Ab-2) (Merck Millipore; DP02; 5 μg/ml). Secondary antibodies (all from Thermo Fisher Scientific ...