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Citations for Millipore Sigma :
301 - 350 of 731 citations for Canine Distemper Virus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus (400 µL) was added together with 5 ug/mL of polybrene (Sigma-Aldrich) and cells were centrifuged at 2,000 rpm for 2 h at 30°C ...
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bioRxiv - Cell Biology 2024Quote: ... The virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were transduced with a mix of supernatant containing virus and polybrene (Sigma H9268 suspended at 4mg/ml in sterile water ...
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bioRxiv - Cell Biology 2020Quote: ... Antigen retrieval was carried out with 0.2 U/mL Chondroitinase ABC (C2905, Sigma, Merck, Darmstadt, Germany) at 37 °C for two hours for DCN ...
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bioRxiv - Microbiology 2020Quote: ... was used to detect the different antigens with anti-rabbit secondary antibodies (1:25000) (Sigma-Aldrich).
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bioRxiv - Physiology 2020Quote: ... sections were dewaxed and rehydrated prior to enzymatic antigen retrieval (trypsin from porcine pancreas (T7168, Sigma)) ...
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bioRxiv - Neuroscience 2021Quote: ... Heat-mediated antigen retrieval was performed by placing slides in 1x sodium citrate buffer (PHR1416, Sigma), pH 6.0 ...
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bioRxiv - Immunology 2023Quote: ... TUNEL staining was conducted following antigen retrieval using the In Situ Cell Death Detection Kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Diphtheria antigen samples were prepared by desorption with sodium citrate (Sigma-Aldrich Chemie GmbH Taufkirchen, Germany) [23] ...
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bioRxiv - Developmental Biology 2023Quote: ... antigen retrieval was performed in 10 mM sodium citrate buffer (pH 6.0; S1804-500G, Sigma-Aldrich) for 12 min at in a microwave at circa 97°C
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed by submerging slides in boiling 10 mM sodium citrate buffer (Sigma-Aldrich), before washing with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated with an alcohol series and incubated in Tris-EDTA antigen retrieval buffer (#E1161, Sigma-Aldrich) for 20 minutes in a pressure cooker at 95°C ...
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bioRxiv - Neuroscience 2023Quote: ... The seven target proteins and antibodies for the sFANSeq panel included Neuronal Nuclei Antigen (NeuN, Millipore Cat# ABN91 ...
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bioRxiv - Physiology 2024Quote: ... and distilled water followed by heat-induced antigen retrieval in citrate buffer (cat# C9999, Sigma-Aldrich) using a 2100 Retriever (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... The filtered virus was mixed with fresh media (30% vol/vol) that included polybrene (Sigma) at a 6 μg/ml final concentration ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 µL of virus was added per well with 10 µg/mL of polybrene (Sigma) in 100 µL of EMEM without antibiotics or serum overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer extension was conducted with 4 units of Avian Myeloblastosis Virus (AMV) reverse transcriptase (Sigma) for 1 hour at 15°C ...
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bioRxiv - Cancer Biology 2019Quote: ... concentrated virus was added to the cells along with 8 ng/mL polybrene (Sigma-Aldrich) and the cells were incubated at 37°C plus 5% CO2 in humidified air ...
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bioRxiv - Cancer Biology 2021Quote: ... virus was washed out and replaced with fresh complete medium with 10μg/ml blasticidin (Sigma). After 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5 mL of freshly thawed virus soup was mixed with polybrene (Cat# H9268, Sigma-Aldrich) to a final concentration of 8 μg/mL and used to infect A549N and HT-1080N cell lines ...
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bioRxiv - Microbiology 2021Quote: ... Virus lytic replication was induced via addition of 2 µg/mL doxycycline hyclate (Sigma-Aldrich). All cells were cultured at 37 °C at 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: Virus titres were quantified by plaque assay in 12-well plates (Millipore Sigma, Cat# Z707775). For the quantification of FCV ...
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bioRxiv - Neuroscience 2019Quote: ... The virus was subsequently concentrated by mixing the filtered supernatant with 50% PEG-8000 (Sigma) in a ratio of 4:1 and placed at 4°C over night ...
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bioRxiv - Immunology 2020Quote: ... sucrose gradient-purified AD169r-GFP virus was transferred to a 100,000 kDa Amicon filter (Millipore), then buffer exchanged with 1× PBS ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The virus particles were then concentrated to 300µl using Amicon Ultra Centrifugal Filters (UFC910024 SIGMA) by spinning at 1000g at 4°C for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... harvested virus was added onto target cells in the presence of 8μg/ml polybrene (Millipore). For in vivo experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were infected with virus-containing media using Polybrene reagent (TR-1003-G; EMD Millipore) according to the manufacturer’s instructions and selected for 48 hr in 2 μg/mL of puromycin.
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bioRxiv - Immunology 2020Quote: ... We generated HAdV-lactoferrin complexes by incubating the virus with 40 μg lactoferrin (Sigma-Aldrich) for 30 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Unabsorbed virus was removed and 2 ml of culture medium supplemented with 1% methylcellulose (Sigma) were added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... Virus-containing supernatant was concentrated using Amicon Ultra-15 100kDa MWCO centrifugal filters (Millipore, UFC910024). For siRNA knockdown experiments ...
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bioRxiv - Cell Biology 2022Quote: ... Undiluted virus was added dropwise to cells with 10 μg/mL polybrene (Sigma-Aldrich; TR1003G), and incubated at 37°C for 24 hrs ...
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free DMEM supplemented with .3% BSA (Sigma), 100U/ml pen/strep (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free MEM supplemented with 3% BSA (Sigma), 100U/mL of penicillin and 100ug/mL of streptomycin mixture (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... virus stocks were concentrated on a 100 KDa cellulose membrane (Amicon Ultra-15, Merck Millipore) and rinsed three times successively with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... virus at the MOI of 50 was applied with 8 μg/ml Polybrene (Merck Millipore). Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... the virus-containing supernatant was filtered through a 0.45-μm pore size PVDF filter (Millipore), and lentiviral particles were concentrated using the Lenti-X Concentrator (Takara ...
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bioRxiv - Immunology 2022Quote: ... were coated with respective antigen for 2h at RT and then blocked with 5% BSA (Sigma-Aldrich). 50 μl 1:50 diluted plasma sample or monoclonal Abs was added to the wells and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... MEFs were immortalized with SV40 large T-antigen by transfection using GeneJuice transfection reagent (Merck Millipore 70967).
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bioRxiv - Cell Biology 2020Quote: ... Antigen retrieval was performed by microwaving sections in preheated 0.01 M citrate buffer pH 6 (Sigma Aldrich) for 5 min.
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bioRxiv - Biochemistry 2020Quote: ... the antigen was detected using mouse-anti-polyHis conjugated with horseradish peroxidase (HRP) (1:20000, A7058, Sigma) for His-tagged antigens ...
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bioRxiv - Biochemistry 2020Quote: ... for mFc tagged antigen versions or goat-anti-hIgG(Fc) conjugated with HRP (1:70000, A0170, Sigma) if hFc-tagged antigens had to be detected ...
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bioRxiv - Molecular Biology 2022Quote: ... Antigen-antibody complexes were identified using goat anti-mouse AP (1:10,000 dilution of A2179, Sigma-Aldrich) and CDP-Star™ Substrate (T2146 ...
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bioRxiv - Developmental Biology 2022Quote: ... antigen unmasking was performed either in 10 mM sodium citrate buffer (pH 6.0; S1804-500G, Sigma-Aldrich) for 12 min at 97°C ...
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bioRxiv - Neuroscience 2023Quote: ... underwent antigen retrieval for 30 minutes at 98°C in citrate buffer (10 mM citrate, Sigma-Aldrich) 0.05% Tween 20 (pH 6.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... antigen retrieval was performed by boiling the sections in 10 mM sodium citrate and 0.05% Tween20 (Sigma) for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... the antigen-serum-bead mixture was transferred into a 96-well filter HTS plate (Millipore, Bedford, MA). The plate was washed 8 times with Buffer A followed by twice with PBS on a vacuum manifold as described previously [26] ...
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bioRxiv - Microbiology 2023Quote: ... rodentium antigens were produced by homogenization of cultured bacterial pellet in lysis buffer (CellLytic B, Sigma-Aldrich) followed by centrifugation and harvesting of the soluble protein antigens ...
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bioRxiv - Cell Biology 2020Quote: ... Virus stock was filtered by centrifugation using Amicon Ultra-15 Centrifugal filter unit (Sigma, Cat # UFC910096) and resuspended in viral propagation media ...