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Citations for Millipore Sigma :
401 - 450 of 731 citations for Canine Distemper Virus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Harvested media containing the desired virus were filtered through Millex-HV 0.45-µm PVDF filters (Millipore, SLHV033RS) and further concentrated with 100,000 NMWL Amicon Ultra-15 centrifugal filter units (Amicon ...
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bioRxiv - Cell Biology 2021Quote: ... supernatant containing propagated virus was filtered through an Amicon Ultra 15 (100 kDa) centrifugal filter (Millipore Sigma) at ~4000 rpm for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... virus was purified using centrifugal filter devices (Centricon Plus-70 and Biomax 100.000, Millipore Corp., Bedford, MA) and stocks with a titre between 3×109 and 3×1010 viral genome equivalents (vge ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa or U2OS cells were transduced with virus for 48 h with 10 μg/ml polybrene (Sigma), then optimized for protein expression via fluorescence sorting or puromycin selection.
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bioRxiv - Immunology 2022Quote: ... Virus was absorbed for 1 hour at 37 degrees C before 1% (w/v) methylcellulose (Sigma-Aldrich) (10 g methylcellulose in 1L MEM (Corning) ...
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bioRxiv - Microbiology 2022Quote: ... virus was preincubated for 1 hr at 4 °C with 100 μg/mL soluble H (Sigma H4784), HS (Sigma H7640) ...
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bioRxiv - Systems Biology 2022Quote: ... The virus was collected both 2 days and 3 days later and 0.45 μm syringe filters (Millipore) were used to eliminate cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Virus containing media were harvested 48-64 h later and filtered by 0.45 μm Steriflip filter (Millipore). HepG2 and Huh7 cells were infected with 1 ml virus containing medium with 8 μg/ml polybrene for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was replaced with virus-containing medium supplemented with 8 µg/mL of polybrene (Sigma-Aldrich). Approximately 24 hours post-viral treatment ...
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bioRxiv - Immunology 2022Quote: ... The supernatant containing virus particles was collected after 48h and filtered via 0.45 μm filter (Roth/Millipore). Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor ...
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bioRxiv - Microbiology 2023Quote: ... Filtered virus-containing medium was mixed 1:1 with fresh HaCaT media and 1:1000 polybrene (Sigma), and used to transduce HaCaT cells overnight [75] ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15-Cas9 cells were infected with pooled CRISPR library virus using polybrene (8 µg/ml, Sigma) using a low multiplicity of infection (MOI = 0.75 ...
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bioRxiv - Cell Biology 2023Quote: ... 2×106 cells were combined with 300 µL of virus and 0.8 µL/mL polybrene (Millipore, #TR1003G) in the well of a 12-well plate (final volume of 2 mL) ...
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bioRxiv - Microbiology 2023Quote: ... RNA from the supernatant of cell culture infected with the virus was isolated using the Trizol (Sigma) method described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... NF1-MET cells were infected with 500 μL of 0.45 μm filtered virus with polybrene (EMD Millipore), and 48 hours post infection transduced cells were selected for by 2 μg/mL puromycin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus was removed and replaced with normal growth medium containing 1 mg/ml puromycin from Sigma. The puromycin selection was used to kill off any uninfected control cells ...
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bioRxiv - Microbiology 2023Quote: ... the virus suspension was replaced with 1mL/well of overlay medium (OM) containing 1% methylcellulose (Sigma, USA) in MM ...
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bioRxiv - Microbiology 2024Quote: ... 100 TCID50 of the virus in Intesticult media supplemented with 500 μM sodium glycochenodeoxycholate (GCDCA; Sigma, G0759) was added to 5-day differentiated HIE monolayers in triplicate on a 96-well plate ...
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bioRxiv - Molecular Biology 2021Quote: ... The antigen-antibody complexes were visualized using a secondary horseradish peroxidase-conjugated goat anti-mouse IgM antibody (Sigma-Aldrich) at a dilution of 1:10,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... For staining on paraffin sections antigen retrieval was performed by either 1mg/ml trypsin (Sigma, T7168, St. Louis, MO) or 10 μg/ml Proteinase K for 20 minutes at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Antigen retrieval was performed by treatment of tissue sections with porcine trypsin tablets (Cat No. T7168-20TAB, Sigma-Aldrich) for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Labeled antigens were sterilized by filtering through 0.22 µm pore size Spin-X centrifuge tube filters (CLS8160, Millipore Sigma) and stored at 4°C until use ...
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bioRxiv - Immunology 2019Quote: ... scFv secretion levels were analyzed by ELISA after coating with HEL antigen (Sigma-Aldrich, 62971, 4 μg ml-1) in PBS (Thermo ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor proliferation in the R26-GB2/MMTV-PyMT animals was assessed by PCNA (proliferating cell nuclear antigen) immunostaining using the MAB424R antibody (1:10,000; clone p10, Millipore). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... was used to detect the different antigens with anti-rabbit secondary antibodies conjugated to horseradish peroxidase (1:25,000; catalog # A0545, Sigma). Either photographic film or a ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections were deparaffinized and antigen retrieval was achieved by use of heat-induced epitope retrieval with pH 6.0 citrate buffer (Sigma). Tissue sections were stained with H&E ...
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bioRxiv - Immunology 2020Quote: ... were cultured in 24 well plates and stimulated with inactivated SARS-COV-2 antigen (1.2 μg/ml) or PMA (25 ng/ml, cat # P8139; Sigma) and Ionomycin (1 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Pathology 2022Quote: ... Enzymatic antigen retrieval was performed for 10 minutes in a 37°C water bath using pepsin (Sigma-Aldrich, P7000). After rinses ...
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bioRxiv - Bioengineering 2023Quote: ... Sections (5 μm) were deparaffinized and rehydrated and subjected to antigen retrieval using citrate buffer (0.0126 M citric acid, Sigma cat ...
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bioRxiv - Microbiology 2023Quote: Sections were deparaffinized in xylene and rehydrated in a descending ethanol series prior to heat-induced antigen unmasking in Citrate Buffer pH 6.0 (Sigma). After blocking of nonspecific binding in PBS-T containing 10% goat serum and 1% BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... 5μm E14.5 paraffine sections underwent deparaffinization and microwave antigen retrieval in pH 6.0 sodium citrate with 0.05% Tween-20 solution (Sigma-Aldrich), followed by gradual chilling ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue cross-sections were deparaffinized and rehydrated and subjected to antigen retrieval using citrate buffer (0.0126 M citric acid, Cat. No. C-0759, Sigma; 0.0874 M sodium citrate ...
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bioRxiv - Bioengineering 2023Quote: ... The antigen-retrieval buffer used for live-cell lysis was 2× Tris-glycine buffer (Sigma Aldrich, St. Louis, MO) supplemented with 1% SDS and 12 M urea (Sigma Aldrich ...
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bioRxiv - Genetics 2023Quote: Rehydrated histological sections underwent antigen retrieval by incubation with 1 mg/mL pepsin in 0.5 M acetic acid (Millipore) at 37 °C for 30 min ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... UM cells were transduced with virus supplemented with 8 µg/ml polybrene (Sigma Aldrich, Saint Louis, Missouri, USA) and 24 h after transduction ...
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bioRxiv - Cell Biology 2020Quote: ... McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich). After 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... Complementary DNA was reverse transcribed from 1 μg total RNA with Moloney murine leukemia virus reverse transcriptase (Sigma) using dNTP (Promega ...
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bioRxiv - Immunology 2021Quote: ... The swabs were placed into 2ml of virus transport medium (VTM) comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2020Quote: ... the cell culture supernatants were pooled to concentrate the virus 20 times using 10,000 MWCO spin columns (Millipore), and aliquots were stored at −70°C [14] ...
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bioRxiv - Bioengineering 2020Quote: ... virus was collected after 120 hours from both cell lysates and media and purified over iodixanol (Optiprep, Sigma).
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bioRxiv - Biochemistry 2020Quote: ... P2 virus was then harvested by pelleting cells by centrifugation at 4000g and decanting into 5% FBS (Sigma), and then stored in the dark at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2 μg/ml Puromycin (Sigma–Aldrich). After 7 days of selection ...
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bioRxiv - Cell Biology 2022Quote: ... Infectious media containing virus was collected 40 hrs later and filtered with a 0.45μm PES membrane filter (Millipore). Live filtered virus was used to transduce cells with polybrene (10μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Thiol concentration at the same intervals as cells infected with virus was determined by Ellman’s reagent (5,5’-dithio-bis-2-nitrobenzoic acid, DTNB, Sigma) by reading the optical density at 412 nm in a plate reader (Biotek Cytation 5 ...
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bioRxiv - Cancer Biology 2022Quote: ... the virus-containing supernatant was centrifuged 500 x g for 5min and filtered with 0.45um PES filters (Millipore), followed by ultracentrifugation (25,000rpm for 2h) ...
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bioRxiv - Immunology 2022Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...