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Citations for Millipore Sigma :
9901 - 9950 of 10000+ citations for Recombinant Mouse Fgf18 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was incubated overnight at 4 °C with the first antibody (rabbit anti-Tc-Zen1 and anti-Tc-Zen2 as for immunohistochemistry, 1:1,000; mouse anti-Tubulin [Sigma-Aldrich #T7451 ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich) in PBST ...
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bioRxiv - Biochemistry 2021Quote: Antibodies used for Western Blot include mouse monoclonal anti β-actin (clone AC-15, Sigma, St. Louis, MO, United States) at 0.13 μg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... samples were boiled and resolved by SDS-PAGE followed by western blotting with mouse FLAG (1:2,500; F3165, Sigma-Aldrich), rabbit MYC (1:2,000 ...
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bioRxiv - Biochemistry 2021Quote: ... Inputs and eluates were separated by SDS-PAGE and transferred to nitrocellulose for Western blotting with mouse anti-FLAG (1:2,500; Sigma F3165) and rabbit anti-calnexin (1:3,000 ADI-SPA-865 ...
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bioRxiv - Immunology 2021Quote: ... Cytokines and chemokines in the homogenates were quantified using Luminex technology with a MILLIPLEX Map Mouse Cytokine/Chemokine Magnetic Bead Panel 25-plex Immunology Multiplex Assay (Cat. No. MCYTOMAG-70K-PMX, Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... standard Western blot was performed using anti-SST and anti-α-Tubulin (1:8,000, B-5-1-2, mouse monoclonal, Sigma).
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bioRxiv - Pathology 2021Quote: ... Splenocytes were isolated from wild-type mouse spleens and cultured overnight with 100ng/ml LPS and 5ug/ml PHA (Sigma). The cells were then labeled with cell trace violet (CTV ...
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bioRxiv - Cell Biology 2020Quote: ... slices from mouse brains 10 months (300 days) of age or older were stained with Thioflavine S (Thio-S, Sigma). Slices were washed with 0.1 M PB three times ...
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bioRxiv - Cell Biology 2021Quote: ... a mouse monoclonal antibody against a highly conserved epitope of chicken gizzard actin (clone C4) from EMD Millipore (Burlington, MA), and a mouse monoclonal antibody against the Ser19 phosphorylated form of the myosin II regulatory light chain (P-MyoRLC ...
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bioRxiv - Cell Biology 2020Quote: ... electroporated parasites were mixed immediately with 100 μl of reticulocyte-rich blood from a naïve mouse treated with phenylhydrazine (6 mg/ml) (Sigma-Aldrich), incubated at 37°C for 20 min and then injected intraperitoneally into another mouse ...
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bioRxiv - Cell Biology 2020Quote: ... Glycaemia was measured using a glucometer and insulin levels using the Milliplex Map Kit (Mouse Metabolic Magnetic Bead Panel. MMHMAG-44K, Millipore) Milliplex Map Kit ...
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bioRxiv - Cell Biology 2020Quote: ... were washed with 100 μl worm protein lysis buffer and incubated with monoclonal M2 mouse anti-Flag antibody (Sigma, F3165) or monoclonal rabbit anti-c-Myc antibody (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... after which bone marrow cells were collected and labeled with monoclonal anti-mouse CD3 (Sigma, catalog no. SAB4700050, 1: 1000), CD19 (Sigma ...
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bioRxiv - Genetics 2021Quote: ... were superovulated by serial Pregnant Mare Serum Gonadotropin (5 IU per mouse, Prospec Protein Specialists) and human chorionic gonadotropin (5 IU, Millipore) injections 46 h apart ...
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bioRxiv - Immunology 2020Quote: ... then binding was detected with specific alkaline phosphatase-conjugated secondary antibodies (anti-mouse total IgG and anti-monkey total IgG from Sigma and anti-mouse IgG1 and IgG2a from BD Biosciences) ...
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bioRxiv - Neuroscience 2020Quote: ... The mouse anti-GFP-20 antibody was used in GRASP and synaptotagmin experiments (1:100 in PBST-NGS; Sigma-Aldrich); the mouse nc82 antibody was used in synaptotagmin::GFP and DenMark experiments ...
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bioRxiv - Microbiology 2020Quote: ... From these fixed cells the manufacturer’s Duolink PLA Immunofluorescence protocol was followed closely using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Millipore Sigma). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... lysates were pre-cleared for 1 hour (h) with mouse immunoglobulin G (IgG) agarose or rabbit immunoglobulin G (IgG) agarose (Sigma), dependent on the species of antibody used in IP ...
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bioRxiv - Immunology 2020Quote: ... 8-µm-thick tissue sections were rehydrated and Fc-blocked with normal mouse and rat serums in the presence of 0.3% Triton X100 (Sigma-Aldrich). Tissues were stained with F4/80-APC (Clone BM8 ...
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bioRxiv - Molecular Biology 2021Quote: Total splenocytes were isolated from C57BL/6 and mFICD-/- mouse spleens and then treated for 3 days with 20 μg/ml LPS (Sigma), 100 µg/ml heparan sulfate or 2.5 μM thapsigargin (Enzo Life Sciences) ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated with mouse monoclonal Alexa Fluor-488 conjugated antibody against NeuN (1:200, MAB377X, Merck Millipore, MA, USA) or the rabbit polyclonal primary antibody against DISC1 (1:250 ...
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bioRxiv - Immunology 2020Quote: ... Ear homogenate supernatants were assayed for chemokines and cytokines with the Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel kit (Millipore). Samples were collected and analyzed using BioPlex 200 reader (BioRad).
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bioRxiv - Physiology 2020Quote: ... The presence of LPL in the conditioned media was confirmed through Western blotting using a mouse antibody against the FLAG tag (1:5000; Sigma). LPL activity was assessed through an LPL activity assay (see below).
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bioRxiv - Immunology 2021Quote: ... and PBS-treated (n ≥ 3) control human gut xenografts and mouse jejunum were purified using GenElute Mammalian Total RNA kit (Sigma) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... for 1h at RT or addition of AP-conjugated goat anti-mouse IgM or IgA (Abcam and Sigma-Aldrich, respectively) and addition of pNPP substrate (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: Total RNA from human hippocampal and prefrontal cortex tissue and mouse hippocampal tissue was extracted with TRIzol reagent (Sigma-Aldrich) and 1 μg was reverse-transcribed using PrimeScriptTM RT Reagent Kit (perfect Real Time ...
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bioRxiv - Neuroscience 2020Quote: ... SI and protein extracts from human and mouse samples were resolved in SDS-PAGE transferred to Immobilon-P PVDF membranes (Millipore). Membranes were activated with methanol and blocked with 4 % bovine serum albumin (BSA ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated with rabbit anti-TMEM43 polyclonal antibody (1:100, NBP1-84132, Novus) (Ab1-2) and mouse anti-FLAG (1:500, F1804, Sigma) for overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Commercial antibodies used for immunofluorescence included mouse anti-neurofilament (1:200, clone SMI-312, #837904) and mouse anti-βIII-tubulin (1:100, clone TU-20, #MAB1637) from Millipore-Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The flowthrough containing the mouse MLKL(1-158) construct was concentrated via centrifugal ultrafiltration (10 kDa molecular weight cut-off; Millipore) and loaded onto a Superdex 75 Increase 10/300 GL size exclusion column (Cytiva ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were placed in a light-protected humidity-controlled tray and incubated in primary antibody (mouse-anti-Neurofilament 200; clone N52; Sigma) at 1:500 in blocking buffer overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and with 10mM EDTA then incubated with Goat anti plexin-A2-APC (RαD systems) for 40 min in the dark at 25 °C for staining plexin-A2 or with Mouse anti-FLAG (Sigma) for 40 min at 4°C followed by Goat anti mouse IgG H&L (Alexa Fluor®647)(abcam ...
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bioRxiv - Genetics 2020Quote: ... 4 RIMBP1 WT and 4 RIMBP1 KO cerebelli were cut parasagittal (30 um) and stained with antibodies anti-GAD65 (1:1000, mouse, DSHB) and anti-calbindin (1:1000, rabbit, Millipore). Synaptic puncta were imaged using a Nikon confocal microscope (A1RSi+ ...
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bioRxiv - Genetics 2020Quote: Western blotting of RPB-9 in the rpb-9::ollas line (Figure S4B) was performed using a mouse anti-tubulin (clone DM1A, SIGMA) antibody and a rat anti-ollas (NBP1-06713 ...
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bioRxiv - Cell Biology 2019Quote: Samples were assayed for insulin or proinsulin content using ELISA according to the instructions of the manufacturers (Rat/Mouse insulin ELISA, Millipore, #EZRMI-13K ...
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used for immunoblotting: The HA epitope was recognised with a primary mouse monoclonal anti-HA (1:1000; Sigma); the Myc epitope was recognised with a primary mouse monoclonal anti-Myc (1:5000 ...
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bioRxiv - Biophysics 2021Quote: ... The following day (day 0) the medium was changed to mouse embryonic fibroblasts (MEF) medium containing doxycycline (2 ng/ml, Sigma) to initiate the expression of the transgenes and thus ...
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bioRxiv - Cell Biology 2021Quote: ... Immunohistochemistry of frozen sections was carried out via standard techniques with α-Tubulin (T5168, mouse monoclonal, Sigma; diluted 1:1000) and a secondary conjugated antibody at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... Hippocampi were isolated from mouse embryos (E18) or newborn pups (P0) and digested with 0.25% trypsin-EDTA (1x) (Sigma, T4549) at 37°C for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: Primary cultures of mouse astrocytes were grown in vitro in the plates coated with 5 μg/mL fibronectin (Sigma, F2006) for 4-5 days before they were infected with lentivirus ...
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bioRxiv - Immunology 2022Quote: ... and the secondary antibody goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 were obtained from Millipore Sigma. Monoclonal Rac1 antibodies (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 μg of total protein samples were made up to 500 μl total volume with 1% NP-40 buffer and incubated overnight at 4°C with 1 μl of mouse anti-Flag primary antibody (#F1804; Sigma). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse anti-γ-H2AX JBW301 (1:1000 for IB, 1:2500 for IF, 1:5000 for FACS; Millipore, 05-636), mouse anti-γ-H2AX-Alexa Fluor 647 (1:1000 for FACS ...
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bioRxiv - Cancer Biology 2022Quote: ... The primary antibodies used were as follows: mouse anti-phospho-Histone H2A.X (Ser139; clone JBW301; Sigma, 05-636; 1/1,000), mouse tubulin (clone B-5-1-2 ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted 1:1000 in casein and incubated overnight at 4 degrees Celsius with shaking OR Monoclonal Anti-Beta-Actin mouse antibody (Sigma) diluted 1:5000 in casein and incubated at room temperature for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... the acini were incubated with the primary antibody mouse anti-podocalyxin (EMD Millipore, clone 3F2:D8, working dilution 1:200), mouse β1 integrin (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... The pre-cleared lysates were then incubated with 100 µL of antibody/protein A complexed beads (anti-FLAG [mouse, Sigma, F1804] or anti-HA [mouse ...
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bioRxiv - Cell Biology 2022Quote: ... U2OS and SYF (src -/- yes -/- fyn -/-) mouse embryonic fibroblasts (Klinghoffer et al., 1999) were cultured at 37 °C with 5% CO2 in DMEM (Sigma) with 4.5 g/l L-glucose ...
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bioRxiv - Cell Biology 2022Quote: Proximity ligation assay (PLA (53)) was performed using the In Situ Red Starter Kit Mouse/Rabbit (Duolink DUO92101, Sigma Aldrich). The conditions specified by the manufacturer’s instructions were optimized by incubating sections with primary antibodies overnight at 4℃ and increasing incubation time with probes and amplification reagents to 2 hours as previously described (54) ...