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Citations for Millipore Sigma :
9751 - 9800 of 10000+ citations for Recombinant Mouse Fgf18 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 hr at room temperature with mouse antibody against α-tubulin (1:500; Sigma Aldrich, T6199), rabbit antibody against Arl13b (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies (at the indicated dilution) were used for this publication: monoclonal mouse anti-Flag (1:2,000) (M2, Sigma F1804), and monoclonal mouse anti-α-tubulin (1:5,000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the slides were incubated for 1 h at room temperature with the appropriate biotin-conjugated secondary antibody: goat anti-mouse (21538, Merck-Millipore) for CD3 and goat anti-rat (BP-9400 ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred to nitrocellulose membrane and probed with primary antibodies against HA (HA-7 mouse monoclonal, 1:2000 dilution, Sigma; or rabbit polyclonal, 1:1000 dilution, Sigma), α-tubulin (mouse monoclonalDM1A ...
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bioRxiv - Physiology 2021Quote: ... Samples were analyzed using a rat insulin radioimmunoassay that has 100% cross-reactivity for mouse insulin (Millipore-Sigma #ri-13k). Insulin content was measured after acid-ethanol extraction using an insulin ELISA (Stellux Rodent Insulin ELISA ...
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bioRxiv - Physiology 2021Quote: ... Samples were analyzed using a rat insulin radioimmunoassay that has 100% cross-reactivity for mouse insulin (Millipore-Sigma #ri-13k). Insulin content was measured after acid-ethanol extraction using an insulin ELISA (Stellux Rodent Insulin ELISA ...
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bioRxiv - Physiology 2020Quote: The membrane was washed in TBST and incubated with a peroxidase-conjugated secondary antibody (goat, anti-mouse IgG, Sigma-Aldrich) diluted 1 ...
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bioRxiv - Cell Biology 2021Quote: ... The blot was blocked in 5% Milk in tris-buffered saline with 0.1% Tween 20 (TBST) and then incubated in 5% Milk TBST containing mouse anti-DPYD (Sigma, WH0001806M1) and rabbit anti-β-actin (Cell Signaling ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were submitted to the UIC Flow Cytometry Core in order to carry out a 32-plex cytokine/chemokine analysis using the 32-plex Milliplex Map Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore). Results were compared between WT and Δrgg2 or WT and Δrgg3 by applying a student’s t test (p=0.05).
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bioRxiv - Microbiology 2020Quote: ... Serial dilutions of mouse sera were tested for binding to the biotinylated proteins and the bound antibodies detected using HRP-conjugated anti-mouse IgG A4416 (Sigma) and TMB substrate (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... All purified proteins were analyzed by SDS-PAGE followed by Coomassie blue staining as well as by Western blot using a mouse monoclonal antibody against the 6xHis-Tag (Novagen). Protein aliquots were stored at −80⁰C in 50% glycerol (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation reactions were performed using anti-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, F1804, Sigma-Aldrich). The complex of chromatin antibody was captured with protein G beads (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C with the following primary antibodies: mouse anti-tyrosine hydroxylase (TH, 1:1000, MAB318, Millipore), rabbit anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed multiple times in TBS-T for 1h before adding secondary antibody (goat anti-mouse alkaline phosphatase conjugate; Sigma) as above at 1:2500 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to well-plates for exposure to two primary antibodies (RFP antibody 5F8 rat for mCherry, RRID: AB_2336064, Chromotek, 1:1000; and mouse-monoclonal anti-CaMKIIα, RRID: AB_309787, Merck Millipore, 1:300) which were added to 0.1 M PBS-Tx 0.2% containing 5% normal goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... a mixture of primary antibodies in PBS-XD overnight (1 μg/ml rabbit anti-GAD67/65, Frontier; 1:4000 mouse anti-glutamate, ImmunoStar; 1:500 goat anti-ChAT, Millipore), and a mixture of secondary antibodies in PBX-XD for two hours (donkey anti-goat ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Insulin concentration of the supernatants was measured with a mouse/rat insulin ELISA (EZRMI-13K, EMD Millipore, Billerica, MA, USA).
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bioRxiv - Physiology 2020Quote: ... and each circulating hormones were determined using a commercially available ELISA kit for total ghrelin (Rat/Mouse Total Ghrelin ELISA kit, EZRGRT-91K, Millipore), and active ghrelin (Rat/Mouse Total Ghrelin ELISA kit ...
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bioRxiv - Molecular Biology 2020Quote: ... ice cold methanol fixed and 4% dried milk blocked cilia pellets and deciliated cell membranes (excised from Transwell inserts) were labelled for 1 hour at room temperature with a mouse anti-alpha-tubulin antibody (T9026, Sigma) diluted 1:500 in PBST ...
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bioRxiv - Neuroscience 2021Quote: ... and probed with the following antibodies overnight at 4°C: mouse anti-PSD95 (1:2000, Millipore, Cat# MAB1596, RRID: AB_2092365), mouse anti-synaptophysin (1:2000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting plasma supernatant was transferred to a sterile Eppendorf tube and stored at −80 °C until the cytokines were measured using a Milliplex xMAP Mouse High Sensitivity TCell Magnetic Bead Panel kit MHSTCMAG-70K (Millipore) following the kit protocol on a Luminex 200 system with Xponent (v2.7) ...
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bioRxiv - Cancer Biology 2020Quote: ... in 5% BSA-TTBS overnight at 4°C and then HRP-conjugated goat anti-rabbit IgG (Jackson) or goat anti-mouse IgG(Sigma) secondary antibodies for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... on top of 3T3-J2 mouse embryonic fibroblast feeder cells inactivated by mitomycin-C treatment (0.4 μg/ml, Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... Bacteria were washed with PBS and opsonized for 30 minutes at room temperature in a 1:1 solution of mouse serum (Sigma) and cell culture media (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... and resuspended in culture medium (Neurobasal A, B27, Glutamax, 10% FBS, 100 ng/mL neural growth factor; mouse NGF 7.0s, Millipore Sigma). DRG neurons were then seeded on each corresponding PAA hydrogel at approximately 30,000 cells per 250 mm2 growth area to achieve single cell density ...
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bioRxiv - Biophysics 2020Quote: ... these were washed three times with PBS and incubated for 2h with the anti-mouse IgG (whole molecule)-FITC secondary antibody (Sigma). Myonemes ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.1% sodium azide) and incubated with the following primary antibodies: mouse anti-α-tubulin (DM1α) 1:500 (Millipore Sigma) for one hour at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: ... 1:100 mouse anti-ASCL1, Guillemot lab; 1:800 rat anti-BrdU, Abcam ab6326; 1:800 chicken anti-GFAP, Millipore AB5541 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in high-salt TBS + 2% BSA + 0.1% Triton X-100 at 1:4000 (mouse anti-a-tubulin, Sigma #T6199) and 2 µg/mL (rabbit anti-CENP-A ...
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bioRxiv - Cell Biology 2022Quote: ... Islet architecture was analyzed using isolated mouse islets fixed in 4% PFA overnight followed by a 20 min incubation with methylene-blue solution (Sigma) and OCT embedding ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for 24-40h at 4°C in mouse anti-GAD67 (1:1000; Sigma Millipore, catalog #MAB5406), rabbit anti-NeuN (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for 24-40h at 4°C in mouse anti-GAD67 (1:1000; Sigma Millipore, catalog #MAB5406), rabbit anti-NeuN (1:500 ...
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bioRxiv - Immunology 2022Quote: ... the different mouse groups were killed 1 hour after retro-orbital injection of 100 μl of 2% Evans blue (EB) dye (Sigma) and were perfused intracardially with 15 ml of PBS ...
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bioRxiv - Neuroscience 2022Quote: Cytokine levels were measured in spinal cord homogenates by Luminex multiplex bead-based analysis (MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel; Millipore) using the Bio-Plex 200 system (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... Mouse monoclonal antibodies against MAP2 (M4403, IC: 1:100) and mouse monoclonal antibodies against GAPDH (WB: 1:1000) were from Sigma. Mouse monoclonal antibodies against GAD65 (GAD-6 supernatant ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Microbiology 2022Quote: ... Nitrocellulose membrane was washed in TBST for 1h before adding secondary antibody (goat anti-mouse alkaline phosphatase conjugate; catalogue number A3562) (Sigma,) in 5% skim milk TBST at 1:2500 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed as before and 100μl/well rabbit anti-mouse IgG1 or IgG2a conjugated to HRP (Sigma-Aldrich, Germany) diluted to 1 ...
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bioRxiv - Microbiology 2022Quote: ... cells were then incubated in a 1:5000 dilution of a horseradish peroxidase (HRP) conjugated goat anti-mouse IgG (Sigma). Titration of RUBV was performed similarly but with a polyclonal anti-RUBV goat IgG at 1:4000 (LifeSpan BioSciences ...
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bioRxiv - Biophysics 2022Quote: ... followed by incubation in LI-COR blocking buffer containing rabbit polyclonal anti-P2X2 (#APR-003, Alomone labs; 1:2000) and mouse anti-Na+/K+-ATPase (05-369; EMD Merck Millipore) at 4 °C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The sample was then washed twice with 2X SSC and digested by 3 incubations (10 minutes for mouse and 5 min for human) with digestion buffer containing 1% (w/v) sodium-dodecyl-sulfate (SDS, Sigma), 20 mM Tris HCl (Thermo) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were the resolved in a 12% SDS-PAGE gel and transferred on a 0.2 µm nitrocellulose membrane and then immunobloted using mouse FlagM2 antibody (Sigma-Aldrich) diluted at 1/500.
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bioRxiv - Biophysics 2022Quote: ... Secondary antibodies were goat anti-rabbit and anti-mouse Alexa 488 and Alexa 555 conjugated fluorescence at 1:500 (Millipore). The spinal cord sections were directly coverslipped with Dapi mounting media (Vector ...
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bioRxiv - Biophysics 2022Quote: ... Phagosomes in each chamber were incubated with one of the following mouse monoclonal antibodies against kinesin-1 (MAB1614, EMD Millipore), kinesin-2 (ab24626 ...
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bioRxiv - Cell Biology 2022Quote: ... 2013), rabbit anti-Cep97 (Dobbelaere et al., 2020), rabbit anti-Cnn (Dobbelaere et al., 2008) and mouse anti-Actin (Sigma); all primary antibodies were used at 1:500 dilution in blocking solution (Aydogan et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... Smith’s laboratory) mouse ESC lines were routinely cultured without feeders on gelatin-coated plates (0.2% gelatin, Sigma-Aldrich, cat. G1890). Media were changed every 2 days and cells were passaged when approaching confluency (every 2-3 days) ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight at 4 °C with the primary antibodies: mouse anti-Syntaxin-1 (1:500, Sigma, # S0664), chicken anti-GFP (1:3000 ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed three times with Tris-buffered saline and Tween-20 followed by incubation with the peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (both from Millipore) for 1 h at room temperature.
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bioRxiv - Biochemistry 2022Quote: ... 0.3% Triton X-100 in PBS) and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804 ...
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bioRxiv - Cell Biology 2022Quote: ... each strip was incubated with 99 μL PBSBT and 1 μL mouse anti-CNB antibody (Sigma-Aldrich, C0581, 1:100), on the nutator for five hours at room temperature ...