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Citations for Millipore Sigma :
9801 - 9850 of 10000+ citations for Recombinant Mouse Fgf18 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... A mouse anti-myosin heavy chain antibody was used as primary antibody (1:200; Sigma Aldrich, St. Louis, MO, USA) and Alexa 488 donkey anti-mouse IgG antibody as secondary antibody (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... shRNA knockdown vectors for mouse Mdm2 (TRNC0000302276) or human MDM2 (TRCN0000355726, TRCN0000355727, TRCN0000355728, TRCN0000003378 and TRCN0000003380) were from Sigma Aldrich. The pBabe-puro expression vector for CA-AMPK has been described53.
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bioRxiv - Cell Biology 2022Quote: The mouse monoclonal anti-GABAA α1 subunit antibody (clone BD24) (catalog #: MAB339) and mouse monoclonal anti-GABAA β2/β3 antibody (catalog #: 05-474) were obtained from Millipore. The rabbit monoclonal anti-GABAA α1 subunit antibody (catalog # ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.1 % Triton X-100 for 60 min at room temperature and incubated with mouse monoclonal anti-TUBB3 (Sigma; #T8660) (1:1000) ...
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bioRxiv - Neuroscience 2020Quote: Live TTX-treated WT and Fmr1R138Q hippocampal neurons (14-15 DIV) were incubated with a mouse monoclonal anti-N-terminal GluA1 antibody (1:100; Merk-Millipore) for 10 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500 ...
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bioRxiv - Immunology 2020Quote: ... fixed antibodies were detected with horseradish peroxidase-conjugated goat anti-mouse IgA (100 µL / well - 1.5 µg/mL; Sigma-Aldrich) for 1h30 at 37°C and the reaction revealed with of 3,3’-5,5’-tetramethylbenzidine peroxidase substrate (100 µL/well - KPL ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... Slides were placed in a light-protected humidity-controlled tray and incubated in primary antibody (mouse-anti-Neurofilament 200; clone N52; MAB5266; Sigma) at 1:500 in blocking buffer overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were stained with rabbit anti-Actin or rabbit anti-TnI (1:1000; a gift from A. Ferrus) or mouse anti-Tubulin (1:1000, Sigma) and washed with TBS-Triton X (0.1%) ...
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bioRxiv - Neuroscience 2020Quote: ... The proximity ligation reaction and amplification of signal were performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for mouse and rabbit antibodies (Duolink, Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was collected in clean microcentrifuge tube and incubated 2 h at 4°C with a-flag mouse antibody (Sigma), following 1 h incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were then incubated for 1 h with secondary antibody a-mouse HRP conjugate 1:10000 (Cell Signalling) and chemiluminescent substrate ultra (Millipore) was applied for 5 min before camera detection in Sapphire Biomolecular Imager (Azure Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805 ...
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bioRxiv - Immunology 2020Quote: ... the viral supernatant was harvested and used to transduce 25,000 mouse primary keratinocytes seeded in 48-well plates in the presence of 8 µg/ml polybrene (Sigma Aldrich). The next day ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated overnight at 4°C with the following primary antibodies: mouse anti-RELN G10 (MAB5364, Millipore 1:2000) or 142 (for human RELN ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were isolated from a single mouse hippocampus in 2 ml of pre-chilled EZ PREP lysis buffer (NUC-101, Sigma) using a glass Dounce tissue grinder (D8938 ...
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bioRxiv - Neuroscience 2021Quote: ... and APC rat anti-mouse 4D4 (marking resident microglia; 1:1000) in blocking buffer containing 0.2% bovine serum albumin (BSA, Sigma-Aldrich) in HBSS ...
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bioRxiv - Neuroscience 2021Quote: ... Isolated nuclei were labelled in suspension in 1 ml PBS buffer with 1:500 anti-NeuN antibody (Millipore, MAB377, mouse) and 1:250 anti-Sox10 antibody (R&D ...
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bioRxiv - Neuroscience 2021Quote: ... then the Duolink In Situ PLA Probe Anti-Rabbit PLUS and Duolink In Situ PLA Probe Anti-Mouse MINUS PLA probes (Sigma) diluted in Duolink Antibody Diluent were applied ...
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bioRxiv - Cancer Biology 2021Quote: ... The PLA was performed using reagents supplied in the Duolink in situ Red PLA Mouse/Rabbit kit (Sigma-Aldrich, DUO92101) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Isolated nuclei were labelled in suspension in 1 ml PBS buffer with 1:500 anti-NeuN antibody (Millipore, MAB377, mouse) and 1:250 anti-Sox10 antibody (R&D ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were incubated with OXT and AVP primary antibodies (mouse anti-oxytoxin, Gainer Lab, NIH, PS3845,60 and rabbit anti-vasopressin, Millipore PC234L) diluted in 0.1 % saponin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate, Merck Millipore). Digital images were acquired on a ChemiDoc XRS System (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were blocked with 5% bovine serum albumin (BSA) and then probed with the following primary antibodies - mouse anti-GluN2A (1:1000, Millipore), rabbit anti-GluN2B (1:1000 ...
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bioRxiv - Microbiology 2021Quote: Western blot analyses of 3xFLAG-tagged proteins or IgaA were carried out as described previously (Pontel and Soncini, 2009; Pérez Audero et al., 2010) with mouse anti-FLAG monoclonal (Sigma-Aldrich) or rabbit polyclonal anti-IgaA antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... Bound scFv were detected using murine mAb 9E10 which recognizes the C-terminal c-myc tag (1:50 diluted in 2% MPBST) and a goat anti-mouse serum conjugated with horseradish peroxidase (HRP) (A0168, Sigma) (1:42000 dilution in 2% MPBST) ...
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bioRxiv - Cancer Biology 2021Quote: ... The Millipore Mouse Cytokine 32-plex kit was used to measure the serum concentration of 32 cytokines (Cat# MCYTMAG-70K-PX32, Millipore). xPONENT (Luminex ...
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bioRxiv - Bioengineering 2020Quote: ... or mouse monoclonal β-actin antibody (1:1000 dilution; catalog no. catalog no. MAB 1501; Merck Millipore, Burlington, MA, USA) at room temperature for 1 hour ...
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bioRxiv - Genetics 2022Quote: ... The slices then incubated with primary antibodies with denoted dilutions overnight at 4°C (anti-Iba1 goat polyclonal, Abcam ab5076, 1:200; anti-HNA mouse monoclonal, Millipore MAB1281 ...
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bioRxiv - Neuroscience 2022Quote: All samples were immunostained for fluorescence activated cell sorting (FACS) with mouse anti-NeuN conjugated to PE (EMD Millipore, FCMAB317PE) at a dilution of 1:500 with incubation for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were then pre-cleared using 25 μL of Protein A agarose beads (Pierce) and then incubated with 25 μL of mouse anti-V5 agarose beads (Sigma) and washed with RIPA buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Horseradish peroxidase conjugated secondary antibodies were added to the membranes (anti-rabbit, Invitrogen; 31460 and anti-mouse EMD Millipore; AP308P). Membranes were developed with ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-mouse IgG (H+L) HRP conjugated (Chemicon International AP308P) and Anti-Rabbit IgG (whole molecule)–Peroxidase (Sigma Aldrich A9169). Chemiluminescence detection was done with SuperSignal West Pico or Femto (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... R26-CreERT2/R26-Confetti mice received intraperitoneal injections of tamoxifen (TAM; 4mg/25 grams mouse weight, Sigma, St. Louis, MO) dissolved in 10% ethanol/corn oil (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking in 5% BSA, membranes were incubated with Anti-FASN rabbit antibody (#3189, CST) antibody or mouse anti-tubulin antibody (Sigma). Protein was next incubated with HRP-conjugated secondary antibodies and was detected using chemiluminescence.
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bioRxiv - Plant Biology 2022Quote: ... A 1–month–old ICR mouse (SLAC laboratory animal) was injected with 50 μg GST–CAR9 protein emulsified with Complete Freund’s adjuvant (F5881, Sigma–Aldrich). Two weeks later ...
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bioRxiv - Neuroscience 2019Quote: ... and or Alexa Flour 488 anti-mouse IgG (1:800, A11001, Life) at room temperature for 1h and mounted with DAPI (F6057, Sigma). The sections were observed using fluorescence microscope (Olympus BX35 ...
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bioRxiv - Biochemistry 2019Quote: The custom designed CompoZr® Zinc Finger Nuclease Plasmid targeted to exon 3 of the mouse ECH1 gene on chromosome 7 was obtained from Sigma. In-vitro transcription of the ZFN mRNA was performed following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... containing 4% PFA (EM Sciences) and 0.1% Triton X-100 and stained with a mouse antibody to α-tubulin (DM1α; SIGMA) and a secondary donkey DyLight 594-conjugated F(ab’)2 mouse IgGs (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Plant Biology 2020Quote: ... Sections were blocked with 5 % bovine serum albumin (BSA) in PBS before their incubation with an anti-HA mouse monoclonal antibody (Sigma) for 2 hours at room temperature ...
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bioRxiv - Genetics 2019Quote: ... Monoubiquitin forms of 8.5 kDa were detected using a mouse monoclonal anti-ubiquitin primary antibody (Sigma, U0508, 1:3000 dilution) and a peroxidase-conjugated AffiniPure goat anti-mouse (Jackson Immuno Research ...
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bioRxiv - Microbiology 2020Quote: ... the Duolink in situ PLA probe anti-mouse MINUS and the in situ detection reagent Green (Sigma-Aldrich, Milan, Italy) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Each slide was then incubated overnight at 4° with a 1:100 dilution of mouse monoclonal anti-a-tubulin primary antibody (clone DM1A, Sigma) in blocking buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes with transferred proteins were blocked/permeabilized for 1 hour in 5% BSA in TBS and incubated with different antibodies (mouse anti-pMLKL, Millipore, rabbit anti-MLKL ...
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bioRxiv - Plant Biology 2019Quote: ... Sections were blocked with 5 % bovine serum albumin (BSA) in PBS and then incubated with 1:50 anti-HA mouse monoclonal antibody (Sigma) in PBS at room temperature for 2 h ...
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bioRxiv - Plant Biology 2019Quote: ... Primary antibody was washed three times with PBS for 15 min and subsequently incubated with 1:40 Alexa 594 - conjugated anti-mouse rabbit monoclonal antibody (Sigma) in PBS at room temperature for 1 h ...
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bioRxiv - Genomics 2019Quote: ... we blocked coverslips with 5% BSA in PBT for 1 hour at room temperature and then incubated coverslips with properly diluted primary antibody (mouse M2 monoclonal ANTI FLAG, 1:800, F1804, Sigma; rabbit polyclonal Tom20 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were blocked for 1 h at RT in 5% skimmed milk powder and 0.2% Tween in PBS before incubating overnight at 4◦C with the primary antibodies mouse anti-TH (1:1000, Millipore) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... Coverslips were blocked for 30 min in 4% Bovine Serum Albumin then incubated overnight with a mouse anti-puromycin monoclonal antibody (Millipore) (1:1000 in 4% BSA) ...