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Citations for Millipore Sigma :
9851 - 9900 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The separated proteins were transferred from the gel to the polyvinyl difluoride (PVDF) membrane (Immobilon-P, 0.45 µm, Merck Millipore Ltd, Ireland) at 200 mA for 1.5 hours ...
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bioRxiv - Cell Biology 2023Quote: ... The unbound portion of this capture step was kept as HIS-free eGFP protein and conjugates and concentrated once on a 10K Amicon column (Millipore Sigma) by spinning for 6-8 minutes at 6000x g ...
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bioRxiv - Cell Biology 2023Quote: ... The peroxidase activity of 3-Amino-9-ethylcarbazole (AEC) was used to visualize the protein expression and the sections were mounted using glycerol gelatin (Sigma-Aldrich). See Table 5 for details on the antibodies used for immunofluorescence ...
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bioRxiv - Developmental Biology 2023Quote: ... All proteins were separated in 5-22% gradient SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (PVDF, Millipore, Billerica, MA) using a semi-dry transfer apparatus (Hoeffer) ...
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bioRxiv - Bioengineering 2023Quote: The purified proteins were concentrated to a concentration of 20 mg/mL using a 10 kDa Amicon® tube (Millipore, UFC9010), and the buffer was exchanged to PBS ...
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bioRxiv - Biophysics 2023Quote: ... The purified proteins were then buffer-exchanged into 20 mM CAPS pH 11 storage buffer using 3K Amicon Ultra centrifugal filters (Millipore, USA). Small aliquots of the protein were frozen in liquid nitrogen at a protein concentration of approximately 1 mM.
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation of nicks were carried out according to standard ChIP protocols using Protein G-coupled Dynabeads coated with monoclonal anti-biotin antibodies (Sigma-Aldrich) antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified protein was then polished by a size exclusion chromatography step using a Superdex ® 200 Increase 10/300 GL (Sigma) in storage buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitates were washed three times with RIPA buffer and Flag-tagged proteins were eluted by two consecutive elution steps with 400 µg/ml Flag-peptide (Sigma-Aldrich) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... each sample with equal amounts of protein was subjected to SDS–PAGE and transferred to a PVDF membrane (Millipore, Burlington, MA). The membranes were incubated with the primary antibody at 4 ℃ overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Equivalent amounts of denatured protein homogenate were resolved by SDS-PAGE on 10% polyacrylamide gels and transferred to a polyvinylidene difluoride membrane (Millipore, USA). After blocking at room temperature for 2 h ...
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bioRxiv - Immunology 2023Quote: ... Protein was further purified by size exclusion chromatography using gel-filtration column (Superose 6 increase 10/300 GL, Cytiva, Sigma-Aldrich) in a buffer containing 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... Aggregation of mutant was carried out with a protein concentration of 100 µM in 10 mM phosphate buffer (Sigma-Aldrich, USA), pH 7.4 at 37ºC without agitation.
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bioRxiv - Cell Biology 2022Quote: ... Selected fraction with pure fusion protein was concentrated and buffer exchanged using an Amicon® Ultra Centrifugal Filter Unit 50MWCO (Millipore) into Extract Compatible Buffer (10 mM HEPES pH7.8 ...
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bioRxiv - Cell Biology 2022Quote: ... Hxk2-3V5 and associated proteins were immunoprecipitated from glass bead extracts (250 μg total protein) using 20 μL of agarose conjugated anti-V5 antibody (Sigma; A7345). The extract and beads were incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were eluted from the beads by 25 µL 1x NuPAGE LDS sample buffer supplemented to 100 mM DTT (Sigma-Aldrich) and heated for 10 min at 70 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... An equal amount of protein (10μg) per sample was separated on 10% SDS polyacrylamide gels and then transferred onto PVDF membranes (Millipore, MA, USA). The membrane was blocked with 4% non-fat milk in TBST buffer at room temperature for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were separated using a 10% sodium dodecyl sulfate polyacrylamide gel and transferred to immobilon polyvinylidene difluoride membranes (EMD Millipore). After blocking for one hour at room temperature in odyssey blocking buffer (LI-COR Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Thirty μg of protein lysates were separated using SDS–polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Immobilion-P, Millipore, IPVH00010). After blocking the membranes in 5% BSA in TBST (1× TBS (Cell Signalling) ...
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 1.5 mg of proteins from each sample was reduced with TCEP (5 mM, room temperature for 10 min, Cat# 580561, Millipore Sigma) then alkylated with chloroacetamide (20 mM ...
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bioRxiv - Cell Biology 2023Quote: ... elegans ChIP reaction or from 50% of a control extract (1 mg protein) were de-crosslinked at 65 °C for at least 4 h with 150 μg/ml Proteinase K (Sigma, #3115887001). DNA from each ChIP reaction or from control extract was isolated using the Qiagen PCR purification kit and diluted to a final volume of 200 μl with (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... The samples for mass spectrometry (MS) analysis were incubated with Dynabeads protein G that were precoupled with the M2-Flag-antibody (Sigma, F1804) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... MBP- and GST-tagged proteins were purified by gel filtration on a Superdex 200 column (Sigma-Aldrich Cat# GE17-5175-01) and concentrated using a 30K Amicon filter.
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bioRxiv - Genomics 2023Quote: ... total cellular ERAP2 protein was enriched by immunoprecipitation and incubated with L-Arginine-7-amido-4-methylcoumarin hydrochloride (R-AMC, A2027, Sigma-Aldrich). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... the column was washed with 20 column volumes of the lysis buffer and the protein was eluted with 3 mM desthiobiotin (Sigma, D1411).
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bioRxiv - Biochemistry 2023Quote: ... One half was used for protein extraction and the other half used for UA quantification using a UA Assay Kit (MAK077, Sigma-Aldrich). Cells were disrupted mechanically ...
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bioRxiv - Physiology 2023Quote: Proteins of interest were labeled with well-validated custom and commercial antibodies: Connexin43 (Cx43; mouse monoclonal antibody, Cat. #: MAB3067, EMD Millipore Corp. ...
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bioRxiv - Cancer Biology 2022Quote: Polyacrylamide gel slices containing bEV and sEV protein lysates were resolved in tris (2-carboxyethyl) phosphine (10 × 10-1 M; Sigma- Aldrich) with ammonium bicarbonate (50 × 10-1 M ...
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bioRxiv - Microbiology 2023Quote: ... and the amount of total protein in each sample was calculated using a 1 mg/ml standard sample of bovine serum albumin (Sigma-Aldrich). To separate the soluble and aggregated proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was filtered with a 0.22 µm filter and concentrated using Amicon Ultra-15 centrifugal filter units (Millipore Sigma Cat#UFC901008). Protein was then loaded onto a Superdex 200 column and 5 mL fractions were collected ...
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bioRxiv - Cancer Biology 2023Quote: ... 80 µg of equivalent proteins from sample were treated with 5mM tris (2-carboxyethyl) phosphine hydrochloride (Sigma-Aldrich Cat No: C4706) for 15 minutes at 37°C to reduce the disulfide bonds ...
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bioRxiv - Immunology 2023Quote: ... The capture matrix was washed three times with PBS and incubated over night with 1:5 diluted plasma samples in a low protein binding MultiScreen® filter plate (Millipore). Depleted plasma was recovered by centrifugation of the filter plate ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane (Immobilion-P, Millipore, Schwalbach, Germany) by semi-dry blotting ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... and SKR-2-containing protein complexes were eluted overnight in 100 µl of the lysis buffer containing 2 mg/mL of 3xFlag peptide (Sigma F4799). Eluted protein samples were digested with trypsin and analyzed by MudPIT in the Mass Spectrometry and Proteomics Facility at Johns Hopkins School of Medicine.
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bioRxiv - Molecular Biology 2023Quote: The dox-inducible A375P cells were treated with doxycycline to express MITF WT and MITF mutant proteins for 24 hours and then treated with 40 µg/ml cycloheximide (Sigma #66819), in presence or absence of 200nM TPA ...
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bioRxiv - Biophysics 2023Quote: ... and the protein of interest was concentrated to 1 mL using a 50 kDa molecular weight cut-off (MWCO) centrifugal filter (Millipore, #UFC505024). Finally ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant proteins were denatured at 95℃ for 10 min and then alkylated with 40 mM IAA (final concentration, Sigma-Alrich) in dark at room temperature for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Pull down of Ago proteins (Ago 1-4) was performed by incubating 500 μg of Flag-GST-T6B peptide 111 (Sigma #M8823) with 80 μl of anti-Flag M2 Magnetic beads (Sigma #M8823 ...
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bioRxiv - Plant Biology 2023Quote: ... protein thiol groups were blocked by a final concentration of freshly balanced 20 mM N-ethylmaleimide (NEM, Sigma 128-53-0) directly added to the lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... produced in house from pAB101 hybridoma cell culture and purified on protein A Sepharose (GE)) or Ab416 (for JCPyV LT, EMD Millipore) primary antibody diluted to 0.2μg/mL in TBST for 4h at RT ...
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bioRxiv - Microbiology 2023Quote: ... from Barcelona (Spain) was filtered through low protein binding 0.22 µm pore size polyethersulfone (PES) membrane filters (Millex-GP, Millipore, Bedford, Massachusetts) to remove bacteria ...
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bioRxiv - Neuroscience 2023Quote: ... the expression of truncated tau protein was induced by cultivating cells in a medium without doxycycline (Sigma-Aldrich, St. Louis, MO) for three days before cell seeding into co-culture ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein quantification was performed using the BCA protein quantification assay (QPro BCA kit, Cyanagen) against a bovine serum albumin (BSA) calibration curve (Sigma Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... Expression of the different FLAG-SET8 proteins in U2OS cells were induced by the addition of 2 µg/ml of tetracyclin (TET) (Sigma-Aldrich) into cell culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... Then 10 mg of protein per sample was separated on 12% SDS-PAGE gels (Sangon Biotech) and subsequently transferred to PVDF membrane (Millipore, Temecula) using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques ...
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bioRxiv - Neuroscience 2023Quote: ... solubilized GluA2 or GluA2-γ2 (1 nmol) and membrane scaffold proteins (50 μL, final concentration 1 mg/mL) (MSP1E3D1, Sigma‒ Aldrich) were added ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were concentrated by centrifugation in 4 mL of Amicon filter concentrators with a 10 kDa cutoff (Millipore Sigma; Burlington, MA). TCEP (1 mM ...
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bioRxiv - Genomics 2023Quote: ... Samples were separated on SDS-PAGE (stacking 4% and resolving (8% top and 15% bottom)) and the protein-resolved gel was transferred onto a PVDF membrane (Millipore, #IPVH00010) in a 1X transfer buffer (25 mM Tris ...
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bioRxiv - Genetics 2023Quote: ... Equal amounts of protein were separated on a SDS-PAGE gel and transferred to an Immobilon-P polyvinylidene difluoride (PDVF) membrane (Millipore, IPVH00010). Membranes were blocked in PBS with 3% milk powder (Sigma ...