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Citations for Millipore Sigma :
9501 - 9550 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The proteins were collected using 800 μL elution buffer (20 mM Na2PO4, 300 mM NaCl, 300 mM imidazole; Sigma Aldrich) and dialyzed against MOPS buffer (30 mM MOPS (3-morpholinopropane-1-sulfonic acid) ...
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bioRxiv - Cancer Biology 2024Quote: ... The target protein mixed with sumo protease and tag were then passed through a second 1 mL Ni-column and the flow-through solution containing target protein was collected and concentrated with an Amicon Ultra-15 centrifugal filter unit (10,000 MWCO) (EMD Millipore) at 4℃ ...
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bioRxiv - Cell Biology 2024Quote: ... and following centrifugation immunoprecipitations were done using supernatant (I mg protein) and anti-FLAG antibody (Sigma #F1804; dilution 1:1000) and Protein G agarose beads (60 µL ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration in the remaining supernatant was assessed using a Bradford reagent assay according to manufacturer’s protocol (Sigma-Aldrich - B6916), and normalized on collected fecal weight and on animal weight.
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bioRxiv - Cell Biology 2024Quote: ... HA-tagged proteins were probed with a 1:1000 dilution of Roche rat anti-HA monoclonal 3F10 primary (Sigma 11867423001) and a 1:5000 dilution of donkey anti-rat DyLight800 (Invitrogen Life Technologies SA5-10032) ...
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bioRxiv - Microbiology 2024Quote: ... mice were intranasally administered with 3 µg of the RSP protein and 5 μg of cholera toxin (CT; Sigma-Aldrich) as immunological adjuvant ...
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bioRxiv - Microbiology 2024Quote: ... The protein was finally eluted with 50 μL of 300 ng/μL 3x FLAG peptide (Sigma-Aldrich, St. Louis, MO) for 45 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Overexpression of the fusion protein was induced by adding 1 mM isopropyl-D-thiogalactopyranoside (IPTG; Sigma Aldrich; St. Louis, MO) to the transformed cell culture ...
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bioRxiv - Microbiology 2024Quote: ... 5 mg of total protein per sample was combined with 100 μL of ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich) and mixed on the end-to-end rotator for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... and the fractions with the highest concentrations of mutant PGA proteins were concentrated into PBS using Amicon Ultra-4 Centrifugal Filters with a 10 kDa molecular weight cutoff (Merck Millipore). This process resulted in a protein with a purity exceeding 70 % and a concentration of approximately 1 mg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... The isolation of nuclear proteins was achieved with the CelLytic™ NuCLEAR™ extraction kit (Sigma Aldrich, St. Louis, MO) using the manufacturer’s recommended protocol ...
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bioRxiv - Biophysics 2024Quote: ... The protein was then concentrated to about 100 μM (Amicon Ultra-15, PLQK Ultracel-PL Membrane, 50 kDa, Merck, Millipore). After size-exclusion chromatography with a Superdex 200 Increase10/300 GL column (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... The resultant protein with an N-terminal His6-tag was concentrated using a 10 kDa Amicon Ultra-15 concentrator (Millipore). Protein concentrations were determined from the absorbance at 280 nm using a molar absorptivity constant (ε280 = 22,920 M-1 cm-1 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (EMD Millipore, IPVH00010), then blocked in Tween 0.1% Tris-buffered saline containing 5% bovine serum albumin (Tocris 5217 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 500 µl of 2 mg/ml proteins were incubated with washed anti-FLAG magnetic beads (Cat. No. M8823, Millipore) at 4 °C for 3 h on rotating wheel ...
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bioRxiv - Molecular Biology 2024Quote: Bl6 mice were immunized with 50 µg of the His-tagged LC purified protein in PBS and TiterMax (Sigma-Aldrich) adjuvant (1:10 ratio ...
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bioRxiv - Cell Biology 2024Quote: ... and screened for expression of the fusion proteins by Western Blotting with an α-FLAG M2-HRP antibody (Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... The eluted protein was concentrated with Amicon Ultra 15 centrifugal filters (Regenerated cellulose 10 000 NMWL; Merck Millipore, Darmstadt, Germany), aliquoted and flash-frozen on dry ice and stored at −80°C until use ...
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bioRxiv - Neuroscience 2024Quote: ... ck1α expression was induced for 16 hours prior to treatment with CHX to stop protein synthesis (10µg/ml) (Sigma-Aldrich). Cells were then harvested and lysed with EB2 supplemented with 5mM EDTA pH 8.0 at the times indicated after CHX addition ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 µg of protein was diluted to a concentration of 1 µg/µl and 2 µg of anti-FLAG M2 antibody (Sigma) was added and incubated overnight at 4°C for IP ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
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bioRxiv - Cell Biology 2024Quote: ... For 3D samples (Figs. 5f, g), protein was extracted using SDS buffer (5% SDS, 50 mM triethylammonium bicarbonate (TEABC; T7408, Sigma)) in combination with mechanical tissue disruption ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then blocked in 6% donkey serum and immunolabeled for protein gene product 9.5 (PGP9.5, 1:500, Millipore-Sigma, SAB4503057-100UG) as a pan-neuronal marker or rabbit-IgG (Abcam ...
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bioRxiv - Biophysics 2024Quote: The NF protein sequences used were adapted from Rattus norvegicus (UniProt accession P19527, P12839, F1LRZ7) and cloned into pET28a(+) (Novagen). A cysteine was introduced near the N-terminus of each sequence to enable the grafting chemistry ...
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bioRxiv - Biochemistry 2024Quote: For the ISD sample preparation each 50 μL aliquot of human CSF was resuspended in 0.2% RapiGest SF (Waters) in 50 mM ammonium bicarbonate 5 with 800 ng of yeast enolase protein (Sigma-Aldrich) added as an internal protein control ...
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bioRxiv - Biochemistry 2024Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was measured using the bichinchoninic acid (BCA, ThermoFischer #23225) method with bovine serum albumin (BSA, Sigma-Aldrich #P0834) as standard ...
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bioRxiv - Synthetic Biology 2024Quote: ... proteins (50 µg from each sample) were first reduced with 10 mM dithiothreitol (DTT) (Sigma-Aldrich, St. Louis, MO, USA) and subsequently alkylated using 50 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: 50 μg of HpARI proteins were incubated with 50 μg of Heparan Sulphate (Heparan sulfate sodium salt from bovine kidney, Sigma) in PBS for 30 min hour at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Fractions that were supposed to contain the protein of the expected size were concentrated by ultrafiltration (10 kDa, UFC9010, Millipore), and aliquots were frozen at -20 °C until use.
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bioRxiv - Developmental Biology 2024Quote: ... and contained 800 μl culture media (Leibovitz’s L15 Medium, no phenol red, Thermo Fisher 21083027; 50 ng/μl Protein A; 0.01% Methyl Cellulose, Sigma 274429). Surface conditions were critical to successful cultures ...
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bioRxiv - Plant Biology 2024Quote: ... 2.5–40 μg of total protein extracts were separated on SDS-PAGE gels and blotted onto PVDF Immobilon-P membrane (Millipore). GFP was detected using the anti-GFP antibody (Mouse monoclonal ...
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bioRxiv - Plant Biology 2020Quote: ... 1 ml of transfected protoplasts were incubated for 12 h for protein expression in the presence of 1 μM DSF (Sigma, USA), and were treated with or without 1 μM flg22 (MoonBiochem ...
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bioRxiv - Plant Biology 2019Quote: ... pulled-down proteins were separated on SDS-PAGE and analyzed by immunoblotting with anti-Flag and anti-Myc antibodies (Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2021Quote: ... Extracts were then clarified at 4’000 × g for 10 min and concentrated to ~4 mg/mL total protein concentration using 3 kDa molecular weight cutoff (MWCO) Centriprep concentrators (EMD Millipore) to account for dilution during preparation ...
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bioRxiv - Cancer Biology 2021Quote: ... and nascent puromycylated proteins was detected by Western blot analysis as above using a mouse anti-puromycin antibody 12D10 (1:3000)(MABE343, Sigma-Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... Free glycans were separated from protein with a 10 kDa molecular weight cutoff centrifugal filter unit (Millipore Amicon Ultra-0.5 #UFC5010). The glycan-containing filtrate was desalted with a PD minitrap G-10 column (Cytiva ...
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bioRxiv - Cell Biology 2019Quote: ... Protein translation was analyzed on the WES automated western blotting system (ProteinSimple) using anti-Puromycin antibody (1:50; Sigma-Aldrich, MABE343).
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bioRxiv - Cell Biology 2020Quote: ... Proteins in the media fraction were precipitated by adding 0.15 ml of 100% trichloroacetic acid (TCA) (Sigma Chemical, St Louis, MO) and incubated on ice for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... and permeabilized with 0.1 % triton X-100 for 10 mins for intracellular staining with a mouse or rabbit monoclonal antibody against the target protein (1h) followed by a secondary antibody conjugated with FITC- or Alexa Fluor 647 (Sigma-Aldrich) (1.100 ...
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bioRxiv - Cell Biology 2020Quote: ... 3 mg of protein was subjected to a pre-clearing step by incubating samples with prewashed salmon sperm DNA/protein-A agarose beads (Millipore Sigma) for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 30 μg of protein from each sample were subjected to SDS-PAGE electrophoresis and transferred to PVDF membrane (Merck Millipore, USA). Then the membrane was blocked in 5% w/v bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... and 20 µg total protein was denatured for 5 min at 70°C in 1X Laemmli Buffer (Sigma Cat # S3401-10VL), resolved on SDS-acrylamide gels and electroblotted to nitrocellulose ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to an Immobilon polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA). The membranes were incubated with primary antibodies overnight at 4⁰C ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged SMOCtail and IFT38 protein fusions were expressed in Rosetta2(DE3)-pLysS cells grown in 2xYT medium (Millipore Sigma, Y2627) at 37°C until the optical density (OD ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 mg of total proteins from whole cell extracts were immunoprecipitated overnight at 4°C using GFP-conjugated magnetic beads (Sigma-Aldrich), then beads were washed three times with immunoprecipitation (IP ...
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bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Cell Biology 2020Quote: ... 1-5ug of antibody was incubated overnight with chromatin and the complex antibody-chromatin was then captured with G protein sepharose beads (Sigma Aldrich) 1h at 4°C ...
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bioRxiv - Genetics 2021Quote: ... purified as previously described18) or HiFi-3xNLS-SpCas9 protein (100 pmol) and sgRNA (300 pmol, IDT) with glycerol (2% of final concentration, Sigma, G2025) and incubating for 15 min at room temperature immediately before electroporation ...
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bioRxiv - Immunology 2021Quote: Egg white immunoglobulins (300 μl) were purified using a commercially available protein A affinity chromatography kit (PURE-1A, Sigma Aldrich Co.). The manufacturer’s instructions were followed ...