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Citations for Millipore Sigma :
9651 - 9700 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 80 µg protein were loaded into a 10 % SDS gel and stained with anti-Tau (clone Tau46, T9450, mouse, 1:1000, Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... proteins were extracted from 2 million cells unstimulated or stimulated with LPS or MSUc supplemented with protease inhibitors (Sigma Aldrich, #11697498001) Protein concentration was measured using the BCA reagent (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... lysates were incubated with 10 ul bed volume per 1 mg protein of anti-myc 9E10-conjugated agarose beads (Sigma Aldrich). Beads were then centrifuged and washed 3X with lysis buffer supplemented with 300mM NaCl ...
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bioRxiv - Biophysics 2021Quote: CoA levels in the protein solution after proteinase K digestion were quantified using a commercial CoA assay kit according to manufacturer’s protocol (Sigma-Aldrich; MAK034). CoA concentration is determined by an enzymatic assay ...
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bioRxiv - Biophysics 2020Quote: The C85A/L194A and C85A/L194A/H234C mutant CLC-ec1 protein crystals were grown in 29% (w/v) PEG400 (Sigma-Aldrich), 200 mM NaCl (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was concentrated to 2-3 mg ml-1 using an Amicon ultracel-100 centrifugal filter unit (EMD Millipore) for amphipol exchange or nanodisc reconstitution ...
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bioRxiv - Biophysics 2020Quote: ... The native samples were then concentrated to a final protein concentration of 0.23 mg mL−1 using 3K MWC Amicon® Ultra-15 Centrifugal Filter Units (Millipore Sigma) (Amicon Filter) ...
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bioRxiv - Cell Biology 2021Quote: ... hCMEC/D3 cells were transduced first by lentiviral particles expressing Cas9 and blue fluorescent protein (BFP) as a transduction marker (LVCAS9BST, Sigma-Aldrich). Cas9-expressing cells were then selected with blasticidin antibiotic at 50 μg/mL for 6 days (Life technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mg of the soluble protein fraction were then transferred into fresh tubes and incubated with 30 μL of monoclonal antiHA agarose beads (Sigma, A2095). Samples were incubated for 2 h with end-over-end rotation at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... surviving colonies were pooled together and resulting cultures were analysed for the expression of target protein following induction with increasing amounts of tetracycline hydrochloride (T3383, Sigma-Aldrich). To generate cells stably expressing nucleus–restricted CDKL5 ...
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bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
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bioRxiv - Cancer Biology 2021Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Puromycin-labeled proteins were detected either by Western blotting or by indirect fluorescence microscopy using a monoclonal anti-puromycin antibody (12D10; Sigma Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... Each recombinant protein was eluted twice in 250 µl of EB containing 1 mM DTT and 250 µg/ml 3xFLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... The fraction of proteins >1 kDa was obtained by dialysis using the Mini Dialysis Kit 1 kDa (Sigma #GE80-6483-94). All kits were applied according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: ... The residual proteins bound to exDNA were digested by 2-h incubation with 0.85 g L−1 proteinase K (Sigma-Aldrich, UK) at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The final proteins were concentrated in a prewashed Amicon Ultra-15 Centrifugal filter (molecular weight cut off 3 kDa; EMD Millipore), flash-frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Cancer Biology 2022Quote: ... and the supernatant containing the sheared chromatin was pre-cleared with Protein G plus-Agarose beads (Cat No. IP04-1.5ML, Merck Millipore, Burlington, USA), which were pre-coated with bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein loading was measured using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000; AC-15, Sigma-Aldrich), nucleolin (1:5000 ...
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bioRxiv - Neuroscience 2022Quote: Each hemibrain was weighed prior to homogenizing in tissue protein extraction reagent (T-PER ThermoScientific; 1mL per 100mg of tissue weight) supplemented with protease and phosphatase inhibitors cocktail (Sigma-Aldrich). Total protein concentration was measured using bicinchoninic acid (BCA ...
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bioRxiv - Systems Biology 2022Quote: ... The proteins were washed twice with acetone and subsequently solubilized using 100 mM ammonium bicarbonate (ABC) (Merck Sigma, Cat. No. 09830). Alternatively ...
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bioRxiv - Physiology 2022Quote: ... Membranes was incubated with the primary antibodies against target proteins: rabbit polyclonal anti-TSG 101 (T5701, Sigma-Aldrich Co., 1:200), rabbit polyclonal anti-CD63 (SAB4301607 ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged proteins were probed with immunoblotting using monoclonal anti-Flag M2-Peroxidase (HRP) antibody (Sigma-Aldrich, Saint Louis, MO, USA) (1/50,000X dilution ...
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bioRxiv - Microbiology 2022Quote: ... Epitope-tagged 3xFLAG proteins were detected using primary antibody anti-FLAG M2 from mouse (diluted 1:4,000, catalogue number F3165; Sigma Aldrich, Germany) and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein (30 μg) were separated in a 10% SDS-polyacrylamide gel and then transferred to PVDF membranes (Millipore, USA). The membranes were blocked in 5% w/v bovine serum albumin before incubation with the primary antibodies at 4°C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... As control for iodide uptake and protein-binding cells were also treated with 30mM sodium perchlorate (Nis inhibitor; NaClO4, Sigma-Aldrich)) and 2 mM methimazole (TPO inhibitor ...
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bioRxiv - Cancer Biology 2022Quote: ... Cysteine containing phosphopeptide was conjugated to carrier protein Limulus hemocyanin using hetero-bifunctional crosslinker m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester (Sigma# 803227). This conjugate was used to immunize New Zealand white rabbits (Charles River Laboratories) ...
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bioRxiv - Biophysics 2022Quote: ... The E protein was cleaved from the EGFP-8xHis fusion by incubation of the beads with 150 units of thrombin (Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Inhibition of further protein biosynthesis and determination of protein half-lives was performed by the replacement of complete medium with complete medium containing 50 µg/ml Cycloheximide (Sigma-Aldrich) 24 h after transfection and the collection of lysates ...
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bioRxiv - Cancer Biology 2022Quote: ... All purified proteins were analyzed by analytical size exclusion chromatography (SEC) (Cytiva) and compared to commercial molecular weight standards (Sigma Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... 40 μg total proteins were loaded to the 8% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gels and transferred onto PVDF membrane (Millipore, IPFL00010) by electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... media was collected and used to measure secreted Cxcl1 protein on an ELISA plate according to the KC/Cxcl1 ELISA kit manufacturer’s instructions (Sigma-Aldrich #RAB0117). Absorbance values at 450 nm were measured with a Tecan plate reader ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were transferred onto nitrocellulose membrane using iBlot2 Gel Transfer Device and immunostained using Neurexin-1 antibody (Millipore ABN161-I) and Tuj1 (Biolegend 801201 ...
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bioRxiv - Microbiology 2020Quote: ... HTF-tagged LsaA and FLAG3-tagged VgaALC proteins were detected using anti-Flag M2 primary (Sigma-Aldrich, F1804; 1:10,000 dilution) antibodies combined with anti-mouse-HRP secondary (Rockland ...
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bioRxiv - Microbiology 2020Quote: ... The collected supernatant was kept on ice until measurements of protein concentrations using Direct Detect® Spectrometer (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Microbiology 2020Quote: ... Cultures were then centrifuged at 2,500 × g for 10 min at 4°C and proteins from the supernatant were precipitated using 10% tricholoroacetic acid (TCA) with acetone (Sigma-Aldrich). 1,700 μl of TCA-acetone was added to 300 μl of culture supernatant and incubated at −20°C for one hour ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Immunology 2020Quote: ... Equal amounts of SG core was subject to immunoprecipitation using anti-G3BP1 antibody (1ug) and isotype specific control antibody and protein A-sepharose beads (Sigma-Aldrich). Samples were incubated at 4⁰C overnight on a rotator and immune complexes recovered by centrifugation and five washes in buffer ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was then added to the Protein G sepharose beads coupled to either HA 12CA5 mouse monoclonal IgG (Sigma Aldrich) or anti-Ty1 (BB2 ...
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bioRxiv - Biochemistry 2020Quote: ... although the gel filtration step and second lyophilization were omitted and the temperature needed to elute the protein from Avicel (Sigma-Aldrich) ranged from 50 to 70 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Unbound dye was removed by 2 washes with 100X excess of protein storage buffer followed by centrifugation in Amicon® Ultra-4 Centrifugal Filter Units (SIGMA MilliPORE). LLPS of dyed and undyed protein was compared as quality control and results were similar (data not shown).
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bioRxiv - Biochemistry 2020Quote: ... The proteins were eluted with phosphate buffered saline containing 300 mM imidazole and 0.1% (v/v) protease inhibitor cocktail (Sigma, P-8849). The proteins were further purified using size-exclusion chromatography ...
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bioRxiv - Systems Biology 2020Quote: ... Urea concentration was subsequently reduced to 2M and proteins were digested overnight at 37°C after the addition of trypsin (Sigma-Aldrich) at a 1:100 ratio ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to each sample and protein disulphide bonds were reduced with 5 µL of 200 mM Tris(2carboxyethyl) phosphine (TCEP, Sigma-Aldrich) solution and incubated at room temperature for 1.5 hours ...
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bioRxiv - Microbiology 2022Quote: ... Proteins in cell lysates were separated by 10% SDS-PAGE and transferred to Immobilon NC membranes (Millipore Merck KGaA, Darmstadt, Germany). The membranes were blocked by incubation with 5% nonfat dry milk in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Isotopically labelled proteins were expressed using Escherichia coli BL21 DE3 cells in M9 minimal media supplemented with 15NH4Cl (Sigma-Aldrich ISOTEC). Chaetomium thermophilum Naa5082-289 containing a C-terminal His-tag was expressed using E ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein (40 μg) were resolved by SDS-PAGE and transferred to a PVDF membrane (#IPVH00010, Millipore, MA, USA). Membranes were blocked with 1×TBST containing 5% dry milk for 2-hr at room temperature and probed with the following antibodies ...
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bioRxiv - Immunology 2022Quote: ... the following: 30 µg of each protein (PopB/PcrH, OprF/I, or both) mixed with curdlan (150 µg/dose) (Sigma-Aldrich) and suspended in saline ...